Restriction map for
pFN21AE5669
(Circular) (Six-base) MAPSORT of: pFN21AE5669.seq Check: 7301 from: 1 to: 6307
pFN21AE5669 6307 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4117 278
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3650 1937
Size: 1713 4594
Acc65I G'GTAC_C
Cuts at: 3628 3628
Size: 6307
AclI AA'CG_TT
Cuts at: 1563 4434 4807 1563
Size: 2871 373 3063
Fragments arranged by size:
3063 2871 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2089 2537 4370 5754 6022 1632
Size: 457 448 1833 1384 268 1917
Fragments arranged by size:
1917 1833 1384 457 448 268
AfeI AGC'GCT
Cuts at: 2714 2714
Size: 6307
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6110
AflIII A'CryG_T
Cuts at: 1203 5206 6183 1203
Size: 4003 977 1327
Fragments arranged by size:
4003 1327 977
AgeI A'CCGG_T
Cuts at: 5138 5138
Size: 6307
AhdI GACnn_n'nnGTC
Cuts at: 5036 5036
Size: 6307
AleI CACnn'nnGTG
Cuts at: 1166 2879 1166
Size: 1713 4594
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2236 1184
Size: 1052 5255
AlwNI CAG_nnn'CTG
Cuts at: 2464 2515 2938 3052 3363 4050 5622 2464
Size: 51 423 114 311 687 1572 3149
Fragments arranged by size:
3149 1572 687 423 311 114 51
ApaLI G'TGCA_C
Cuts at: 4364 5520 4364
Size: 1156 5151
ApoI r'AATT_y
Cuts at: 1493 3616 3842 3994 6103 1493
Size: 2123 226 152 2109 1697
Fragments arranged by size:
2123 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4861 160
Size: 872 3829 1606
Fragments arranged by size:
3829 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
AvaI C'yCGr_G
Cuts at: 1870 1943 2493 3340 3632 1870
Size: 73 550 847 292 4545
Fragments arranged by size:
4545 847 550 292 73
BamHI G'GATC_C
Cuts at: 3637 4004 3637
Size: 367 5940
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3628 5083 618
Size: 325 217 627 1841 1455 1842
Fragments arranged by size:
1842 1841 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2298 3626 1391
Size: 907 1328 4072
Fragments arranged by size:
4072 1328 907
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3036 928
Size: 971 1137 4199
Fragments arranged by size:
4199 1137 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4498 4532 1903
Size: 34 50 34 2477 34 3678
Fragments arranged by size:
3678 2477 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4199 5409 4199
Size: 1210 5097
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6307
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2288 3165
Size: 107 122 71 631 605 337 279 877
Cuts at: 3165 4918 136
Size: 1753 1525
Fragments arranged by size:
1753 1525 877 631 605 337 279 122
107 71
BglII A'GATC_T
Cuts at: 2481 6302 2481
Size: 3821 2486
BlpI GC'TnA_GC
Cuts at: 3724 3724
Size: 6307
Bme1580I G_kGCm'C
Cuts at: 1790 2755 3009 4368 5524 1790
Size: 965 254 1359 1156 2573
Fragments arranged by size:
2573 1359 1156 965 254
BmgBI CAC'GTC
Cuts at: 1318 2071 1318
Size: 753 5554
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4038 4996 442
Size: 416 53 83 399 24 2621 958 1753
Fragments arranged by size:
2621 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3146 4967 1239
Size: 1907 1821 2579
Fragments arranged by size:
2579 1907 1821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4432 5297 5595 4432
Size: 865 298 5144
Fragments arranged by size:
5144 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2336 4970 882
Size: 617 837 2634 2219
Fragments arranged by size:
2634 2219 837 617
BsaAI yAC'GTr
Cuts at: 493 1204 2626 3528 4098 493
Size: 711 1422 902 570 2702
Fragments arranged by size:
2702 1422 902 711 570
BsaBI GATnn'nnATC
Cuts at: 6301 6301
Size: 6307
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4114 4496 5131 275
Size: 53 83 186 936 2581 382 635 1451
Fragments arranged by size:
2581 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2423 4740 5138 5412 5559 5970 2423
Size: 2317 398 274 147 411 2760
Fragments arranged by size:
2760 2317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3517 3547 1178
Size: 30 165 30 2114 30 3938
Fragments arranged by size:
3938 2114 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2756 3052 1186
Size: 1570 296 4441
Fragments arranged by size:
4441 1570 296
BseYI C'CCAG_C
Cuts at: 3536 5510 6006 3536
Size: 1974 496 3837
Fragments arranged by size:
3837 1974 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 2261 3098 3249 1163
Size: 573 303 222 837 151 4221
Fragments arranged by size:
4221 837 573 303 222 151
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2283 3757 4518 5546 1540
Size: 275 186 282 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 282 275 186
BsiHKAI G_wGCw'C
Cuts at: 3576 4368 4453 5524 3576
Size: 792 85 1071 4359
Fragments arranged by size:
4359 1071 792 85
BsmI GAATG_Cn'
Cuts at: 3818 3911 3818
Size: 93 6214
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2755 3009 3576 3626 4368 4453
Size: 399 508 457 254 567 50 742 85
Cuts at: 4453 5524 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 567 508 457 399 254
85 50
BspEI T'CCGG_A
Cuts at: 2423 5970 2423
Size: 3547 2760
BspHI T'CATG_A
Cuts at: 4196 5926 4196
Size: 1730 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2514 3042 3662 844
Size: 1670 528 620 3489
Fragments arranged by size:
3489 1670 620 528
BsrBI CCG'CTC
Cuts at: 1394 1823 4194 1394
Size: 429 2371 3507
Fragments arranged by size:
3507 2371 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2688 4802 4976 65
Size: 1183 1440 2114 174 1396
Fragments arranged by size:
2114 1440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2223 4951 5138 1700
Size: 110 413 2728 187 2869
Fragments arranged by size:
2869 2728 413 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6307
BssHII G'CGCG_C
Cuts at: 1925 2015 2953 1925
Size: 90 938 5279
Fragments arranged by size:
5279 938 90
BssSI C'ACGA_G
Cuts at: 4367 5379 4367
Size: 1012 5295
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6307
BstBI TT'CG_AA
Cuts at: 5111 5111
Size: 6307
BstEII G'GTnAC_C
Cuts at: 1184 2584 1184
Size: 1400 4907
BstXI CCAn_nnnn'nTGG
Cuts at: 3243 3243
Size: 6307
BstYI r'GATC_y
Cuts at: 1972 2481 2973 3637 4004 4389 4406 5858 5944
Size: 509 492 664 367 385 17 1452 86
Cuts at: 5944 5956 6302 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 664 509 492 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3578 3578
Size: 6307
BtgI C'CryG_G
Cuts at: 513 1065 2283 2589 3093 513
Size: 552 1218 306 504 3727
Fragments arranged by size:
3727 1218 552 504 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2014 487
Size: 683 56 788 4780
Fragments arranged by size:
4780 788 683 56
BtsI GCAGTG_nn'
Cuts at: 2218 2811 3018 3382 3823 4617 4637 2218
Size: 593 207 364 441 794 20 3888
Fragments arranged by size:
3888 794 593 441 364 207 20
ClaI AT'CG_AT
Cuts at: 1554 2409 1554
Size: 855 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6272
DraI TTT'AAA
Cuts at: 3611 3611
Size: 6307
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5314 809
Size: 507 3998 1802
Fragments arranged by size:
3998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2280 2289
Size: 54 1425 50 275 18 327 123 9
Cuts at: 2289 2706 2784 2808 3198 3754 4643 6272 8
Size: 417 78 24 390 556 889 1629 43
Fragments arranged by size:
1629 1425 889 556 417 390 327 275
123 78 54 50 43 24 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2280 3754 1537
Size: 275 468 1474 4090
Fragments arranged by size:
4090 1474 468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3017 3191 4237 1353
Size: 1664 174 1046 3423
Fragments arranged by size:
3423 1664 1046 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3510 3581 4894 5268 5414 387
Size: 1064 138 1921 71 1313 374 146 1280
Fragments arranged by size:
1921 1313 1280 1064 374 146 138 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 2537 3146 4370 4967 5754 6022
Size: 393 457 448 609 1224 597 787 268
Cuts at: 6022 1239
Size: 1524
Fragments arranged by size:
1524 1224 787 609 597 457 448 393
268
EcoO109I rG'GnC_Cy
Cuts at: 3240 3240
Size: 6307
EcoRI G'AATT_C
Cuts at: 3616 3994 3616
Size: 378 5929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3053 3053
Size: 6307
FspI TGC'GCA
Cuts at: 4813 4813
Size: 6307
HaeII r_GCGC'y
Cuts at: 1678 2716 5454 1678
Size: 1038 2738 2531
Fragments arranged by size:
2738 2531 1038
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 4948 4980 5022
Size: 32 135 32 826 32 2518 32 42
Cuts at: 5022 5054 1373
Size: 32 2626
Fragments arranged by size:
2626 2518 826 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3651 3897 669
Size: 988 281 1713 246 3079
Fragments arranged by size:
3079 1713 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6307
HpaI GTT'AAC
Cuts at: 3897 3897
Size: 6307
KpnI G_GTAC'C
Cuts at: 3632 3632
Size: 6307
MfeI C'AATT_G
Cuts at: 3906 3906
Size: 6307
MluI A'CGCG_T
Cuts at: 6183 6183
Size: 6307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2634 3450 5421 5605 1305
Size: 1329 816 1971 184 2007
Fragments arranged by size:
2007 1971 1329 816 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2708 2810 10
Size: 54 1425 343 876 102 3507
Fragments arranged by size:
3507 1425 876 343 102 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 4265 4624 4783 518
Size: 648 1713 1386 359 159 2042
Fragments arranged by size:
2042 1713 1386 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2365 2789 3085 3723 4400 5548 5793 1606
Size: 759 424 296 638 677 1148 245 2120
Fragments arranged by size:
2120 1148 759 677 638 424 296 245
NaeI GCC'GGC
Cuts at: 2225 2225
Size: 6307
NcoI C'CATG_G
Cuts at: 513 1065 2589 3093 513
Size: 552 1524 504 3727
Fragments arranged by size:
3727 1524 552 504
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6307
NgoMIV G'CCGG_C
Cuts at: 2223 2223
Size: 6307
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6307
NotI GC'GGCC_GC
Cuts at: 3754 3754
Size: 6307
NspI r_CATG'y
Cuts at: 1132 1229 2217 3665 5210 1132
Size: 97 988 1448 1545 2229
Fragments arranged by size:
2229 1545 1448 988 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6307
PciI A'CATG_T
Cuts at: 5206 5206
Size: 6307
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6307
PfoI T'CCnGG_A
Cuts at: 2667 2667
Size: 6307
PmeI GTTT'AAAC
Cuts at: 3611 3611
Size: 6307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 5889 5921 4376
Size: 32 1481 32 4762
Fragments arranged by size:
4762 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6307
PsiI TTA'TAA
Cuts at: 3877 3877
Size: 6307
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3467 3499 3467
Size: 32 6275
PstI C_TGCA'G
Cuts at: 830 2088 2511 2997 3025 3368 3405 3659 830
Size: 1258 423 486 28 343 37 254 3478
Fragments arranged by size:
3478 1258 486 423 343 254 37 28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6307
PvuII CAG'CTG
Cuts at: 2365 3085 2365
Size: 720 5587
SalI G'TCGA_C
Cuts at: 1936 3649 1936
Size: 1713 4594
SbfI CC_TGCA'GG
Cuts at: 3659 3659
Size: 6307
ScaI AGT'ACT
Cuts at: 2633 4555 6267 2633
Size: 1922 1712 2673
Fragments arranged by size:
2673 1922 1712
SexAI A'CCwGG_T
Cuts at: 2202 2673 2202
Size: 471 5836
SfcI C'TryA_G
Cuts at: 826 1045 2084 2507 2635 2993 3021 3364 3370
Size: 219 1039 423 128 358 28 343 6
Cuts at: 3370 3401 3655 4790 5471 5662 826
Size: 31 254 1135 681 191 1471
Fragments arranged by size:
1471 1135 1039 681 423 358 343 254
219 191 128 31 28 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2288 2288
Size: 6307
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6307
SmaI CCC'GGG
Cuts at: 1872 2495 3634 1872
Size: 623 1139 4545
Fragments arranged by size:
4545 1139 623
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4411 5312 5574 820
Size: 197 926 2468 901 262 1553
Fragments arranged by size:
2468 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3528 4098 493
Size: 3035 570 2702
Fragments arranged by size:
3035 2702 570
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6307
SphI G_CATG'C
Cuts at: 1132 2217 3665 1132
Size: 1085 1448 3774
Fragments arranged by size:
3774 1448 1085
SspI AAT'ATT
Cuts at: 5 52 4231 5
Size: 47 4179 2081
Fragments arranged by size:
4179 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6307
StyI C'CwwG_G
Cuts at: 513 1065 2376 2589 3093 3178 3236 3746 513
Size: 552 1311 213 504 85 58 510 3074
Fragments arranged by size:
3074 1311 552 510 504 213 85 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4334 4351 4504 4689 5134 325
Size: 1255 2754 17 153 185 445 1498
Fragments arranged by size:
2754 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2370 2631 3444
Size: 274 80 33 51 1443 393 261 813
Cuts at: 3444 4553 6265 96
Size: 1109 1712 138
Fragments arranged by size:
1712 1443 1109 813 393 274 261 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2556 2588 6267 6299 2556
Size: 32 3679 32 2564
Fragments arranged by size:
3679 2564 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2675 1316
Size: 1359 4948
XbaI T'CTAG_A
Cuts at: 3643 3643
Size: 6307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2399 2725 2399
Size: 326 5981
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6307
XmaI C'CCGG_G
Cuts at: 1870 2493 3632 1870
Size: 623 1139 4545
Fragments arranged by size:
4545 1139 623
XmnI GAAnn'nnTTC
Cuts at: 3251 4436 3251
Size: 1185 5122
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4115 276
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII
SanDI SapI SfoI SrfI SwaI