Restriction map for
pFN21AE5667
(Circular) (Six-base) MAPSORT of: pFN21AE5667.seq Check: 774 from: 1 to: 6274
pFN21AE5667 6274 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4084 278
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3617 1937
Size: 1680 4594
Acc65I G'GTAC_C
Cuts at: 3595 3595
Size: 6274
AclI AA'CG_TT
Cuts at: 1563 4401 4774 1563
Size: 2838 373 3063
Fragments arranged by size:
3063 2838 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 3207 3305 4337 5721 5989 1632
Size: 443 1132 98 1032 1384 268 1917
Fragments arranged by size:
1917 1384 1132 1032 443 268 98
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6077
AflIII A'CryG_T
Cuts at: 1203 5173 6150 1203
Size: 3970 977 1327
Fragments arranged by size:
3970 1327 977
AgeI A'CCGG_T
Cuts at: 5105 5105
Size: 6274
AhdI GACnn_n'nnGTC
Cuts at: 5003 5003
Size: 6274
AleI CACnn'nnGTG
Cuts at: 1166 2210 1166
Size: 1044 5230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6274
AlwNI CAG_nnn'CTG
Cuts at: 2332 4017 5589 2332
Size: 1685 1572 3017
Fragments arranged by size:
3017 1685 1572
ApaLI G'TGCA_C
Cuts at: 4331 5487 4331
Size: 1156 5118
ApoI r'AATT_y
Cuts at: 1493 2762 2921 3024 3275 3441 3476 3583 3809
Size: 1269 159 103 251 166 35 107 226
Cuts at: 3809 3961 6070 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1269 251 226 166 159 152
107 103 35
AseI AT'TA_AT
Cuts at: 160 1032 2520 3171 4828 160
Size: 872 1488 651 1657 1606
Fragments arranged by size:
1657 1606 1488 872 651
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
AvaI C'yCGr_G
Cuts at: 1870 1943 3269 3599 1870
Size: 73 1326 330 4545
Fragments arranged by size:
4545 1326 330 73
BamHI G'GATC_C
Cuts at: 3604 3971 3604
Size: 367 5907
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2025 3595 5050 618
Size: 325 217 627 238 1570 1455 1842
Fragments arranged by size:
1842 1570 1455 627 325 238 217
BanII G_rGCy'C
Cuts at: 1391 3593 1391
Size: 2202 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2499 2881 3312 928
Size: 971 600 382 431 3890
Fragments arranged by size:
3890 971 600 431 382
BbvCI CC'TCA_GC
Cuts at: 2010 2010
Size: 6274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4465 4499 1903
Size: 34 2528 34 3678
Fragments arranged by size:
3678 2528 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2594 4166 5376 2594
Size: 1572 1210 3492
Fragments arranged by size:
3492 1572 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6274
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4885 136
Size: 107 122 71 631 605 3213 1525
Fragments arranged by size:
3213 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6269 6269
Size: 6274
BlpI GC'TnA_GC
Cuts at: 3691 3691
Size: 6274
Bme1580I G_kGCm'C
Cuts at: 1790 4335 5491 1790
Size: 2545 1156 2573
Fragments arranged by size:
2573 2545 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6274
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 2578 4005
Size: 416 53 83 399 24 897 264 1427
Cuts at: 4005 4963 442
Size: 958 1753
Fragments arranged by size:
1753 1427 958 897 416 399 264 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2796 3530 4934 1239
Size: 1557 734 1404 2579
Fragments arranged by size:
2579 1557 1404 734
Bpu10I CC'TnA_GC
Cuts at: 2010 2010
Size: 6274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3437 3453 4399 5264 5562 3437
Size: 16 946 865 298 4149
Fragments arranged by size:
4149 946 865 298 16
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4937 882
Size: 617 3438 2219
Fragments arranged by size:
3438 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4065 493
Size: 711 2861 2702
Fragments arranged by size:
2861 2702 711
BsaBI GATnn'nnATC
Cuts at: 6268 6268
Size: 6274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 4081 4463 5098
Size: 53 83 186 936 482 2066 382 635
Cuts at: 5098 275
Size: 1451
Fragments arranged by size:
2066 1451 936 635 482 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4707 5105 5379 5526 5937 4707
Size: 398 274 147 411 5044
Fragments arranged by size:
5044 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6049
Fragments arranged by size:
6049 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2807 1186
Size: 1621 4653
BseYI C'CCAG_C
Cuts at: 5477 5973 5477
Size: 496 5778
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5701
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3724 4485 5513 1540
Size: 275 186 1723 761 1028 2301
Fragments arranged by size:
2301 1723 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4335 4420 5491 4335
Size: 85 1071 5118
Fragments arranged by size:
5118 1071 85
BsmI GAATG_Cn'
Cuts at: 3785 3878 3785
Size: 93 6181
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3593 4335 4420 5491 1391
Size: 399 1803 742 85 1071 2174
Fragments arranged by size:
2174 1803 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5937 5937
Size: 6274
BspHI T'CATG_A
Cuts at: 4163 5893 4163
Size: 1730 4544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3629 844
Size: 2785 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4161 1394
Size: 429 2338 3507
Fragments arranged by size:
3507 2338 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4769 4943 65
Size: 1183 3521 174 1396
Fragments arranged by size:
3521 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4918 5105 1700
Size: 110 3108 187 2869
Fragments arranged by size:
3108 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3019 96
Size: 2923 3351
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6274
BssSI C'ACGA_G
Cuts at: 4334 5346 4334
Size: 1012 5262
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6274
BstBI TT'CG_AA
Cuts at: 2042 5078 2042
Size: 3036 3238
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6274
BstYI r'GATC_y
Cuts at: 1972 3604 3971 4356 4373 5825 5911 5923 6269
Size: 1632 367 385 17 1452 86 12 346
Cuts at: 6269 1972
Size: 1977
Fragments arranged by size:
1977 1632 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4783
Fragments arranged by size:
4783 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5535
Fragments arranged by size:
5535 683 56
BtsI GCAGTG_nn'
Cuts at: 3790 4584 4604 3790
Size: 794 20 5460
Fragments arranged by size:
5460 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6239
DraI TTT'AAA
Cuts at: 2343 3578 2343
Size: 1235 5039
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5281 809
Size: 507 3965 1802
Fragments arranged by size:
3965 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3721 4610 6239
Size: 54 1425 50 275 18 1891 889 1629
Cuts at: 6239 8
Size: 43
Fragments arranged by size:
1891 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3721 1537
Size: 275 1909 4090
Fragments arranged by size:
4090 1909 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3346 4204 1353
Size: 1993 858 3423
Fragments arranged by size:
3423 1993 858
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4861 5235 5381 387
Size: 1064 138 3272 374 146 1280
Fragments arranged by size:
3272 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2796 3207 3305 3530 4337 4934
Size: 393 443 721 411 98 225 807 597
Cuts at: 4934 5721 5989 1239
Size: 787 268 1524
Fragments arranged by size:
1524 807 787 721 597 443 411 393
268 225 98
EcoO109I rG'GnC_Cy
Cuts at: 2282 2282
Size: 6274
EcoRI G'AATT_C
Cuts at: 2762 3583 3961 2762
Size: 821 378 5075
Fragments arranged by size:
5075 821 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2303 3431 2303
Size: 1128 5146
FspI TGC'GCA
Cuts at: 4780 4780
Size: 6274
HaeII r_GCGC'y
Cuts at: 1678 5421 1678
Size: 3743 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4915 4947 4989 5021 1373
Size: 32 135 32 3343 32 42 32 2626
Fragments arranged by size:
3343 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2402 3618 3864 669
Size: 988 281 464 1216 246 3079
Fragments arranged by size:
3079 1216 988 464 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6274
HpaI GTT'AAC
Cuts at: 3864 3864
Size: 6274
KpnI G_GTAC'C
Cuts at: 3599 3599
Size: 6274
MfeI C'AATT_G
Cuts at: 3873 3873
Size: 6274
MluI A'CGCG_T
Cuts at: 6150 6150
Size: 6274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5388 5572 1305
Size: 4083 184 2007
Fragments arranged by size:
4083 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4452
Fragments arranged by size:
4452 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 4232 4591 4750 518
Size: 648 1044 2022 359 159 2042
Fragments arranged by size:
2042 2022 1044 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2332 2670 3046 3327 3690 4367 5515 5760
Size: 726 338 376 281 363 677 1148 245
Cuts at: 5760 1606
Size: 2120
Fragments arranged by size:
2120 1148 726 677 376 363 338 281
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4783
Fragments arranged by size:
4783 939 552
NdeI CA'TA_TG
Cuts at: 387 2163 387
Size: 1776 4498
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6274
NotI GC'GGCC_GC
Cuts at: 3721 3721
Size: 6274
NspI r_CATG'y
Cuts at: 1132 1229 2427 3093 3632 5177 1132
Size: 97 1198 666 539 1545 2229
Fragments arranged by size:
2229 1545 1198 666 539 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6274
PciI A'CATG_T
Cuts at: 5173 5173
Size: 6274
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6274
PmeI GTTT'AAAC
Cuts at: 3578 3578
Size: 6274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 5856 5888 4343
Size: 32 1481 32 4729
Fragments arranged by size:
4729 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2282 2282
Size: 6274
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6274
PsiI TTA'TAA
Cuts at: 3844 3844
Size: 6274
PstI C_TGCA'G
Cuts at: 830 3626 830
Size: 2796 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6274
PvuII CAG'CTG
Cuts at: 2332 2670 3046 2332
Size: 338 376 5560
Fragments arranged by size:
5560 376 338
SalI G'TCGA_C
Cuts at: 1936 3616 1936
Size: 1680 4594
SbfI CC_TGCA'GG
Cuts at: 3626 3626
Size: 6274
ScaI AGT'ACT
Cuts at: 3058 4522 6234 3058
Size: 1464 1712 3098
Fragments arranged by size:
3098 1712 1464
SfcI C'TryA_G
Cuts at: 826 1045 3622 4757 5438 5629 826
Size: 219 2577 1135 681 191 1471
Fragments arranged by size:
2577 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6274
SmaI CCC'GGG
Cuts at: 1872 3601 1872
Size: 1729 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3269 3416 3468 4378 5279 5541
Size: 197 926 1326 147 52 910 901 262
Cuts at: 5541 820
Size: 1553
Fragments arranged by size:
1553 1326 926 910 901 262 197 147
52
SnaBI TAC'GTA
Cuts at: 493 4065 493
Size: 3572 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6274
SphI G_CATG'C
Cuts at: 1132 3093 3632 1132
Size: 1961 539 3774
Fragments arranged by size:
3774 1961 539
SspI AAT'ATT
Cuts at: 5 52 3068 4198 5
Size: 47 3016 1130 2081
Fragments arranged by size:
3016 2081 1130 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6274
StyI C'CwwG_G
Cuts at: 513 1065 2004 2561 3713 513
Size: 552 939 557 1152 3074
Fragments arranged by size:
3074 1152 939 557 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4301 4318 4471 4656 5101 325
Size: 1255 2721 17 153 185 445 1498
Fragments arranged by size:
2721 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 3019 3056
Size: 274 80 33 51 1443 324 718 37
Cuts at: 3056 4520 6232 96
Size: 1464 1712 138
Fragments arranged by size:
1712 1464 1443 718 324 274 138 80
51 37 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6234 6266 6234
Size: 32 6242
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6274
XbaI T'CTAG_A
Cuts at: 3610 3610
Size: 6274
XhoI C'TCGA_G
Cuts at: 1943 3269 1943
Size: 1326 4948
XmaI C'CCGG_G
Cuts at: 1870 3599 1870
Size: 1729 4545
XmnI GAAnn'nnTTC
Cuts at: 3155 3477 3562 4403 3155
Size: 322 85 841 5026
Fragments arranged by size:
5026 841 322 85
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4082 276
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI