Restriction map for
pFN21AE5665
(Circular) (Six-base) MAPSORT of: pFN21AE5665.seq Check: 7921 from: 1 to: 6289
pFN21AE5665 6289 bp
With 182 enzymes: SgfI *
October 12, 2018 18:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4099 278
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3632 1937
Size: 1695 4594
Acc65I G'GTAC_C
Cuts at: 3610 3610
Size: 6289
AclI AA'CG_TT
Cuts at: 1563 4416 4789 1563
Size: 2853 373 3063
Fragments arranged by size:
3063 2853 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4352 5736 6004 1632
Size: 2720 1384 268 1917
Fragments arranged by size:
2720 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6092
AflIII A'CryG_T
Cuts at: 1203 2679 3225 3258 5188 6165 1203
Size: 1476 546 33 1930 977 1327
Fragments arranged by size:
1930 1476 1327 977 546 33
AgeI A'CCGG_T
Cuts at: 5120 5120
Size: 6289
AhdI GACnn_n'nnGTC
Cuts at: 5018 5018
Size: 6289
AleI CACnn'nnGTG
Cuts at: 1166 2510 2787 1166
Size: 1344 277 4668
Fragments arranged by size:
4668 1344 277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2805 3543 1184
Size: 1621 738 3930
Fragments arranged by size:
3930 1621 738
AlwNI CAG_nnn'CTG
Cuts at: 2576 2705 2942 4032 5604 2576
Size: 129 237 1090 1572 3261
Fragments arranged by size:
3261 1572 1090 237 129
ApaI G_GGCC'C
Cuts at: 2016 2016
Size: 6289
ApaLI G'TGCA_C
Cuts at: 4346 5502 4346
Size: 1156 5133
ApoI r'AATT_y
Cuts at: 1493 3598 3824 3976 6085 1493
Size: 2105 226 152 2109 1697
Fragments arranged by size:
2109 2105 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4843 160
Size: 872 3811 1606
Fragments arranged by size:
3811 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
AvaI C'yCGr_G
Cuts at: 1870 1943 3250 3614 1870
Size: 73 1307 364 4545
Fragments arranged by size:
4545 1307 364 73
BamHI G'GATC_C
Cuts at: 2220 3619 3986 2220
Size: 1399 367 4523
Fragments arranged by size:
4523 1399 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2197 2326 2534 2607 2789
Size: 325 217 627 410 129 208 73 182
Cuts at: 2789 3334 3490 3610 5065 618
Size: 545 156 120 1455 1842
Fragments arranged by size:
1842 1455 627 545 410 325 217 208
182 156 129 120 73
BanII G_rGCy'C
Cuts at: 1391 2016 2820 2874 3179 3311 3471 3608 1391
Size: 625 804 54 305 132 160 137 4072
Fragments arranged by size:
4072 804 625 305 160 137 132 54
BbeI G_GCGC'C
Cuts at: 3494 3494
Size: 6289
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3136 3372 928
Size: 971 1237 236 3845
Fragments arranged by size:
3845 1237 971 236
BbvCI CC'TCA_GC
Cuts at: 2073 2112 2590 2073
Size: 39 478 5772
Fragments arranged by size:
5772 478 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4480 4514 1903
Size: 34 50 34 2459 34 3678
Fragments arranged by size:
3678 2459 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4181 5391 4181
Size: 1210 5079
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6289
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4900 136
Size: 107 122 71 631 605 3228 1525
Fragments arranged by size:
3228 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6284 6284
Size: 6289
BlpI GC'TnA_GC
Cuts at: 2957 3706 2957
Size: 749 5540
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2202 3339 4350 5506 1790
Size: 226 186 1137 1011 1156 2573
Fragments arranged by size:
2573 1156 1137 1011 226 186
BmgBI CAC'GTC
Cuts at: 1318 2653 3228 3286 3370 1318
Size: 1335 575 58 84 4237
Fragments arranged by size:
4237 1335 575 84 58
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2258 3518 4020
Size: 416 53 83 399 24 841 1260 502
Cuts at: 4020 4978 442
Size: 958 1753
Fragments arranged by size:
1753 1260 958 841 502 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 6289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3485 4949 1239
Size: 2246 1464 2579
Fragments arranged by size:
2579 2246 1464
Bpu10I CC'TnA_GC
Cuts at: 2073 2112 2590 3207 2073
Size: 39 478 617 5155
Fragments arranged by size:
5155 617 478 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2416 4414 5279 5577 2416
Size: 1998 865 298 3128
Fragments arranged by size:
3128 1998 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2895 3344 4952 882
Size: 617 1396 449 1608 2219
Fragments arranged by size:
2219 1608 1396 617 449
BsaAI yAC'GTr
Cuts at: 493 1204 3259 4080 493
Size: 711 2055 821 2702
Fragments arranged by size:
2702 2055 821 711
BsaBI GATnn'nnATC
Cuts at: 6283 6283
Size: 6289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2261 2828 3069
Size: 53 83 186 936 528 200 567 241
Cuts at: 3069 3491 3513 3575 4096 4478 5113 275
Size: 422 22 62 521 382 635 1451
Fragments arranged by size:
1451 936 635 567 528 521 422 382
241 200 186 83 62 53 22
BsaWI w'CCGG_w
Cuts at: 4722 5120 5394 5541 5952 4722
Size: 398 274 147 411 5059
Fragments arranged by size:
5059 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6064
Fragments arranged by size:
6064 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2093 3116 1186
Size: 904 3 1023 4359
Fragments arranged by size:
4359 1023 904 3
BseYI C'CCAG_C
Cuts at: 2032 2068 2713 3197 3568 5492 5988 2032
Size: 36 645 484 371 1924 496 2333
Fragments arranged by size:
2333 1924 645 496 484 371 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2421 2534 2783 3437 3487 1163
Size: 573 685 113 249 654 50 3965
Fragments arranged by size:
3965 685 654 573 249 113 50
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3001 3739 4500 5528 1540
Size: 275 186 1000 738 761 1028 2301
Fragments arranged by size:
2301 1028 1000 761 738 275 186
BsiHKAI G_wGCw'C
Cuts at: 2820 3179 4350 4435 5506 2820
Size: 359 1171 85 1071 3603
Fragments arranged by size:
3603 1171 1071 359 85
BsmI GAATG_Cn'
Cuts at: 3800 3893 3800
Size: 93 6196
BsmBI CGTCTCn'nnnn_
Cuts at: 2252 2252
Size: 6289
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2202 2820 2874 3179 3311 3339
Size: 399 226 186 618 54 305 132 28
Cuts at: 3339 3471 3608 4350 4435 5506 1391
Size: 132 137 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 618 399 305 226 186
137 132 132 85 54 28
BspEI T'CCGG_A
Cuts at: 5952 5952
Size: 6289
BspHI T'CATG_A
Cuts at: 3019 4178 5908 3019
Size: 1159 1730 3400
Fragments arranged by size:
3400 1730 1159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3644 844
Size: 2800 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3303 4176 1394
Size: 429 1480 873 3507
Fragments arranged by size:
3507 1480 873 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2446 3250 4784 4958 65
Size: 1183 1198 804 1534 174 1396
Fragments arranged by size:
1534 1396 1198 1183 804 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2531 4933 5120 1700
Size: 110 721 2402 187 2869
Fragments arranged by size:
2869 2402 721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6289
BssHII G'CGCG_C
Cuts at: 1925 3383 1925
Size: 1458 4831
BssSI C'ACGA_G
Cuts at: 4349 5361 4349
Size: 1012 5277
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3406 3415 794
Size: 2612 9 3668
Fragments arranged by size:
3668 2612 9
BstBI TT'CG_AA
Cuts at: 5093 5093
Size: 6289
BstEII G'GTnAC_C
Cuts at: 1184 2497 1184
Size: 1313 4976
BstYI r'GATC_y
Cuts at: 1972 2220 2892 3619 3986 4371 4388 5840 5926
Size: 248 672 727 367 385 17 1452 86
Cuts at: 5926 5938 6284 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 727 672 385 367 346 248
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2039 2039
Size: 6289
BtgI C'CryG_G
Cuts at: 513 1065 2202 2271 2349 2688 2971 3010 3355
Size: 552 1137 69 78 339 283 39 345
Cuts at: 3355 513
Size: 3447
Fragments arranged by size:
3447 1137 552 345 339 283 78 69
39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2199 2602 487
Size: 683 56 973 403 4174
Fragments arranged by size:
4174 973 683 403 56
BtsI GCAGTG_nn'
Cuts at: 3542 3805 4599 4619 3542
Size: 263 794 20 5212
Fragments arranged by size:
5212 794 263 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6254
DraI TTT'AAA
Cuts at: 3593 3593
Size: 6289
DraIII CAC_nnn'GTG
Cuts at: 2551 3406 2551
Size: 855 5434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5296 809
Size: 507 3980 1802
Fragments arranged by size:
3980 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 2998 3549
Size: 54 1425 50 275 18 438 730 551
Cuts at: 3549 3736 4625 6254 8
Size: 187 889 1629 43
Fragments arranged by size:
1629 1425 889 730 551 438 275 187
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2998 3736 1537
Size: 275 1186 738 4090
Fragments arranged by size:
4090 1186 738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2180 4219 1353
Size: 827 2039 3423
Fragments arranged by size:
3423 2039 827
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2679 4876 5250 5396 387
Size: 1064 138 1090 2197 374 146 1280
Fragments arranged by size:
2197 1280 1090 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2818 3177 2818
Size: 359 5930
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3485 4352 4949 5736 6004 1239
Size: 393 1853 867 597 787 268 1524
Fragments arranged by size:
1853 1524 867 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2762 2762
Size: 6289
EcoO109I rG'GnC_Cy
Cuts at: 2012 2013 2116 2252 2694 3555 2012
Size: 1 103 136 442 861 4746
Fragments arranged by size:
4746 861 442 136 103 1
EcoRI G'AATT_C
Cuts at: 3598 3976 3598
Size: 378 5911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 6289
FspI TGC'GCA
Cuts at: 4795 4795
Size: 6289
HaeII r_GCGC'y
Cuts at: 1678 3494 5436 1678
Size: 1816 1942 2531
Fragments arranged by size:
2531 1942 1816
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4930 4962 5004 5036 1373
Size: 32 135 32 3358 32 42 32 2626
Fragments arranged by size:
3358 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3633 3879 669
Size: 988 281 1695 246 3079
Fragments arranged by size:
3079 1695 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6289
HpaI GTT'AAC
Cuts at: 3879 3879
Size: 6289
KasI G'GCGC_C
Cuts at: 3490 3490
Size: 6289
KpnI G_GTAC'C
Cuts at: 3614 3614
Size: 6289
MfeI C'AATT_G
Cuts at: 3888 3888
Size: 6289
MluI A'CGCG_T
Cuts at: 6165 6165
Size: 6289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2661 5403 5587 1305
Size: 1356 2742 184 2007
Fragments arranged by size:
2742 2007 1356 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 10
Size: 54 1425 343 438 4029
Fragments arranged by size:
4029 1425 438 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 2510 2651 2787 3024 3138 4247
Size: 648 1287 57 141 136 237 114 1109
Cuts at: 4247 4606 4765 518
Size: 359 159 2042
Fragments arranged by size:
2042 1287 1109 648 359 237 159 141
136 114 57
MspA1I CmG'CkG
Cuts at: 1606 2072 2111 2717 2973 3128 3581 3705 4382
Size: 466 39 606 256 155 453 124 677
Cuts at: 4382 5530 5775 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 606 466 453 256 245
155 124 39
NaeI GCC'GGC
Cuts at: 2533 2533
Size: 6289
NarI GG'CG_CC
Cuts at: 3491 3491
Size: 6289
NcoI C'CATG_G
Cuts at: 513 1065 2271 2688 3010 513
Size: 552 1206 417 322 3792
Fragments arranged by size:
3792 1206 552 417 322
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6289
NgoMIV G'CCGG_C
Cuts at: 2531 2531
Size: 6289
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6289
NotI GC'GGCC_GC
Cuts at: 2998 3736 2998
Size: 738 5551
NspI r_CATG'y
Cuts at: 1132 1229 2584 2683 3443 3647 5192 1132
Size: 97 1355 99 760 204 1545 2229
Fragments arranged by size:
2229 1545 1355 760 204 99 97
PasI CC'CwG_GG
Cuts at: 1348 2016 2056 1348
Size: 668 40 5581
Fragments arranged by size:
5581 668 40
PciI A'CATG_T
Cuts at: 2679 5188 2679
Size: 2509 3780
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2857 1108
Size: 1749 4540
PmeI GTTT'AAAC
Cuts at: 3593 3593
Size: 6289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 5871 5903 4358
Size: 32 1481 32 4744
Fragments arranged by size:
4744 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2694 3555 2694
Size: 861 5428
PshAI GACnn'nnGTC
Cuts at: 1613 3012 3119 1613
Size: 1399 107 4783
Fragments arranged by size:
4783 1399 107
PsiI TTA'TAA
Cuts at: 3859 3859
Size: 6289
PspOMI G'GGCC_C
Cuts at: 2012 2012
Size: 6289
PstI C_TGCA'G
Cuts at: 830 2142 3641 830
Size: 1312 1499 3478
Fragments arranged by size:
3478 1499 1312
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6289
PvuII CAG'CTG
Cuts at: 2072 2111 2717 2072
Size: 39 606 5644
Fragments arranged by size:
5644 606 39
SacI G_AGCT'C
Cuts at: 2820 3179 2820
Size: 359 5930
SacII CC_GC'GG
Cuts at: 2974 2974
Size: 6289
SalI G'TCGA_C
Cuts at: 1936 3631 1936
Size: 1695 4594
SanDI GG'GwC_CC
Cuts at: 2694 3555 2694
Size: 861 5428
SbfI CC_TGCA'GG
Cuts at: 3641 3641
Size: 6289
ScaI AGT'ACT
Cuts at: 4537 6249 4537
Size: 1712 4577
SexAI A'CCwGG_T
Cuts at: 2757 3402 2757
Size: 645 5644
SfcI C'TryA_G
Cuts at: 826 1045 2045 2138 3637 4772 5453 5644 826
Size: 219 1000 93 1499 1135 681 191 1471
Fragments arranged by size:
1499 1471 1135 1000 681 219 191 93
SfoI GGC'GCC
Cuts at: 3492 3492
Size: 6289
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6289
SmaI CCC'GGG
Cuts at: 1872 3252 3616 1872
Size: 1380 364 4545
Fragments arranged by size:
4545 1380 364
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2395 4393 5294 5556 820
Size: 197 926 452 1998 901 262 1553
Fragments arranged by size:
1998 1553 926 901 452 262 197
SnaBI TAC'GTA
Cuts at: 493 4080 493
Size: 3587 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6289
SphI G_CATG'C
Cuts at: 1132 3647 1132
Size: 2515 3774
SrfI GCCC'GGGC
Cuts at: 3252 3252
Size: 6289
SspI AAT'ATT
Cuts at: 5 52 4213 5
Size: 47 4161 2081
Fragments arranged by size:
4161 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6289
StyI C'CwwG_G
Cuts at: 513 1065 2271 2659 2688 3010 3728 513
Size: 552 1206 388 29 322 718 3074
Fragments arranged by size:
3074 1206 718 552 388 322 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2008 3537 4316 4333 4486 4671 5116
Size: 1255 428 1529 779 17 153 185 445
Cuts at: 5116 325
Size: 1498
Fragments arranged by size:
1529 1498 1255 779 445 428 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2676 4535 6247
Size: 274 80 33 51 1443 699 1859 1712
Cuts at: 6247 96
Size: 138
Fragments arranged by size:
1859 1712 1443 699 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6249 6281 6249
Size: 32 6257
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6289
XbaI T'CTAG_A
Cuts at: 3625 3625
Size: 6289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3187 3187
Size: 6289
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6289
XmaI C'CCGG_G
Cuts at: 1870 3250 3614 1870
Size: 1380 364 4545
Fragments arranged by size:
4545 1380 364
XmnI GAAnn'nnTTC
Cuts at: 4418 4418
Size: 6289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4097 276
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstXI BstZ17I EcoRV FseI FspAI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SapI SfiI SwaI