Restriction map for
pFN21AE5660
(Circular) (Six-base) MAPSORT of: pFN21AE5660.seq Check: 478 from: 1 to: 6229
pFN21AE5660 6229 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2162 2162
Size: 6229
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4039 278
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2430 3572 1937
Size: 493 1142 4594
Fragments arranged by size:
4594 1142 493
Acc65I G'GTAC_C
Cuts at: 2693 2733 3550 2693
Size: 40 817 5372
Fragments arranged by size:
5372 817 40
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2149 3142 4292 5676 5944 1632
Size: 517 993 1150 1384 268 1917
Fragments arranged by size:
1917 1384 1150 993 517 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 5128 6105 1203
Size: 3925 977 1327
Fragments arranged by size:
3925 1327 977
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 4958 4958
Size: 6229
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6229
AlwNI CAG_nnn'CTG
Cuts at: 2213 3972 5544 2213
Size: 1759 1572 2898
Fragments arranged by size:
2898 1759 1572
ApaLI G'TGCA_C
Cuts at: 4286 5442 4286
Size: 1156 5073
ApoI r'AATT_y
Cuts at: 1493 2193 3538 3764 3916 6025 1493
Size: 700 1345 226 152 2109 1697
Fragments arranged by size:
2109 1697 1345 700 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4783 160
Size: 872 3751 1606
Fragments arranged by size:
3751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 2703 3554 1870
Size: 73 760 851 4545
Fragments arranged by size:
4545 851 760 73
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2505 2693 2733 3069 3313
Size: 325 217 627 718 188 40 336 244
Cuts at: 3313 3550 5005 618
Size: 237 1455 1842
Fragments arranged by size:
1842 1455 718 627 336 325 244 237
217 188 40
BanII G_rGCy'C
Cuts at: 1391 2769 3049 3548 1391
Size: 1378 280 499 4072
Fragments arranged by size:
4072 1378 499 280
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3247 3451 928
Size: 971 1348 204 3706
Fragments arranged by size:
3706 1348 971 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4420 4454 1903
Size: 34 2483 34 3678
Fragments arranged by size:
3678 2483 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4121 5331 4121
Size: 1210 5019
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2283 4840 136
Size: 107 122 71 631 605 611 2557 1525
Fragments arranged by size:
2557 1525 631 611 605 122 107 71
BglII A'GATC_T
Cuts at: 3196 6224 3196
Size: 3028 3201
BlpI GC'TnA_GC
Cuts at: 3646 3646
Size: 6229
Bme1580I G_kGCm'C
Cuts at: 1790 2344 2796 3316 4290 5446 1790
Size: 554 452 520 974 1156 2573
Fragments arranged by size:
2573 1156 974 554 520 452
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3395 3413 3960
Size: 416 53 83 399 24 1978 18 547
Cuts at: 3960 4918 442
Size: 958 1753
Fragments arranged by size:
1978 1753 958 547 416 399 83 53
24 18
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2235 2939 4889 1239
Size: 980 16 704 1950 2579
Fragments arranged by size:
2579 1950 980 704 16
Bpu10I CC'TnA_GC
Cuts at: 3048 3048
Size: 6229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2470 2927 4354 5219 5517 2470
Size: 457 1427 865 298 3182
Fragments arranged by size:
3182 1427 865 457 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2229 4892 882
Size: 617 730 2663 2219
Fragments arranged by size:
2663 2219 730 617
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 6223 6223
Size: 6229
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4036 4418 5053 275
Size: 53 83 186 936 2503 382 635 1451
Fragments arranged by size:
2503 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4662 5060 5334 5481 5892 4662
Size: 398 274 147 411 4999
Fragments arranged by size:
4999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2677 2707 2943 2973 1178
Size: 30 165 30 1274 30 236 30 4434
Fragments arranged by size:
4434 1274 236 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2609 2678 2951 1186
Size: 1423 69 273 4464
Fragments arranged by size:
4464 1423 273 69
BseYI C'CCAG_C
Cuts at: 2020 3450 5432 5928 2020
Size: 1430 1982 496 2321
Fragments arranged by size:
2321 1982 1430 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2803 3503 1163
Size: 573 1067 700 3889
Fragments arranged by size:
3889 1067 700 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3679 4440 5468 1540
Size: 275 186 1678 761 1028 2301
Fragments arranged by size:
2301 1678 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2417 3414 4290 4375 5446 2417
Size: 997 876 85 1071 3200
Fragments arranged by size:
3200 1071 997 876 85
BsmI GAATG_Cn'
Cuts at: 3444 3740 3833 3444
Size: 296 93 5840
Fragments arranged by size:
5840 296 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2344 2417 2769 2796 3049 3316 3414
Size: 399 554 73 352 27 253 267 98
Cuts at: 3414 3548 4290 4375 5446 1391
Size: 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 554 399 352 267 253
134 98 85 73 27
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2162 3445 3584 844
Size: 1318 1283 139 3489
Fragments arranged by size:
3489 1318 1283 139
BsrBI CCG'CTC
Cuts at: 1394 1823 2911 4116 1394
Size: 429 1088 1205 3507
Fragments arranged by size:
3507 1205 1088 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4724 4898 65
Size: 1183 3476 174 1396
Fragments arranged by size:
3476 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4873 5060 1700
Size: 110 3063 187 2869
Fragments arranged by size:
3063 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6229
BssSI C'ACGA_G
Cuts at: 4289 5301 4289
Size: 1012 5217
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6229
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6229
BstXI CCAn_nnnn'nTGG
Cuts at: 2231 2947 2231
Size: 716 5513
BstYI r'GATC_y
Cuts at: 1972 2598 3196 3559 3926 4311 4328 5780 5866
Size: 626 598 363 367 385 17 1452 86
Cuts at: 5866 5878 6224 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 626 598 385 367 363 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2880 513
Size: 552 939 876 3862
Fragments arranged by size:
3862 939 876 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5490
Fragments arranged by size:
5490 683 56
BtsI GCAGTG_nn'
Cuts at: 2344 3515 3745 4539 4559 2344
Size: 1171 230 794 20 4014
Fragments arranged by size:
4014 1171 794 230 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2515 2550 529
Size: 35 1951 35 4208
Fragments arranged by size:
4208 1951 35 35
DraI TTT'AAA
Cuts at: 2541 3533 2541
Size: 992 5237
DraIII CAC_nnn'GTG
Cuts at: 2803 2803
Size: 6229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2548 3676 4565
Size: 54 1425 50 275 18 718 1128 889
Cuts at: 4565 6194 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1128 889 718 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3676 1537
Size: 275 1864 4090
Fragments arranged by size:
4090 1864 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2068 2335 2369 3161 4159 1353
Size: 715 267 34 792 998 3423
Fragments arranged by size:
3423 998 792 715 267 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4816 5190 5336 387
Size: 1064 138 3227 374 146 1280
Fragments arranged by size:
3227 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2219 2235 2939 3142 4292 4889
Size: 393 517 70 16 704 203 1150 597
Cuts at: 4889 5676 5944 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1150 787 704 597 517 393 268
203 70 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2680 2680
Size: 6229
EcoO109I rG'GnC_Cy
Cuts at: 2210 2258 2969 2210
Size: 48 711 5470
Fragments arranged by size:
5470 711 48
EcoRI G'AATT_C
Cuts at: 3538 3916 3538
Size: 378 5851
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 5376 1678
Size: 3698 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2095 2127 4870 4902 4944
Size: 32 135 32 523 32 2743 32 42
Cuts at: 4944 4976 1373
Size: 32 2626
Fragments arranged by size:
2743 2626 523 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2431 3573 3819 669
Size: 988 281 493 1142 246 3079
Fragments arranged by size:
3079 1142 988 493 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KpnI G_GTAC'C
Cuts at: 2697 2737 3554 2697
Size: 40 817 5372
Fragments arranged by size:
5372 817 40
MfeI C'AATT_G
Cuts at: 2041 3828 2041
Size: 1787 4442
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3108 5343 5527 1305
Size: 1803 2235 184 2007
Fragments arranged by size:
2235 2007 1803 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2550 10
Size: 54 1425 343 718 3689
Fragments arranged by size:
3689 1425 718 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4187 4546 4705 518
Size: 648 3021 359 159 2042
Fragments arranged by size:
3021 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2654 3060 3287 3645 4322 5470 5715
Size: 478 570 406 227 358 677 1148 245
Cuts at: 5715 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 570 478 406 358 245
227
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4738
Fragments arranged by size:
4738 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 3587 5132 1132
Size: 97 2358 1545 2229
Fragments arranged by size:
2358 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6229
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2213 3469 1108
Size: 1105 1256 3868
Fragments arranged by size:
3868 1256 1105
PfoI T'CCnGG_A
Cuts at: 3462 3462
Size: 6229
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 5811 5843 4298
Size: 32 1481 32 4684
Fragments arranged by size:
4684 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2210 2969 2210
Size: 759 5470
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PstI C_TGCA'G
Cuts at: 830 3581 830
Size: 2751 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 2084 2654 3060 3287 2084
Size: 570 406 227 5026
Fragments arranged by size:
5026 570 406 227
SalI G'TCGA_C
Cuts at: 1936 2429 3571 1936
Size: 493 1142 4594
Fragments arranged by size:
4594 1142 493
SapI GCTCTTCn'nnn_
Cuts at: 2369 3161 2369
Size: 792 5437
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 3321 4477 6189 3321
Size: 1156 1712 3361
Fragments arranged by size:
3361 1712 1156
SexAI A'CCwGG_T
Cuts at: 3219 3219
Size: 6229
SfcI C'TryA_G
Cuts at: 826 1045 2010 3009 3577 4712 5393 5584 826
Size: 219 965 999 568 1135 681 191 1471
Fragments arranged by size:
1471 1135 999 965 681 568 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 2705 3556 1872
Size: 833 851 4545
Fragments arranged by size:
4545 851 833
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2449 2906 4333 5234 5496 820
Size: 197 926 506 457 1427 901 262 1553
Fragments arranged by size:
1553 1427 926 901 506 457 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3587 1132
Size: 2455 3774
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 3007 1517
Size: 1490 4739
StyI C'CwwG_G
Cuts at: 513 1065 2004 3169 3668 513
Size: 552 939 1165 499 3074
Fragments arranged by size:
3074 1165 939 552 499
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2048 2523 4256 4273 4426 4611 5056
Size: 1255 468 475 1733 17 153 185 445
Cuts at: 5056 325
Size: 1498
Fragments arranged by size:
1733 1498 1255 475 468 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3319 4475 6187
Size: 274 80 33 51 1443 1342 1156 1712
Cuts at: 6187 96
Size: 138
Fragments arranged by size:
1712 1443 1342 1156 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2016 2048 2429 2461 2943 2975 3021 3053 6189
Size: 32 381 32 482 32 46 32 3136
Cuts at: 6189 6221 2016
Size: 32 2024
Fragments arranged by size:
3136 2024 482 381 46 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3221 1316
Size: 1905 4324
XbaI T'CTAG_A
Cuts at: 3565 3565
Size: 6229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3211 3376 3491 3211
Size: 165 115 5949
Fragments arranged by size:
5949 165 115
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 2703 3554 1870
Size: 833 851 4545
Fragments arranged by size:
4545 851 833
XmnI GAAnn'nnTTC
Cuts at: 3254 4358 3254
Size: 1104 5125
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4037 276
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI