Restriction map for
pFN21AE5658
(Circular) (Six-base) MAPSORT of: pFN21AE5658.seq Check: 6660 from: 1 to: 6172
pFN21AE5658 6172 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2588 2588
Size: 6172
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3982 278
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2895 3515 1937
Size: 958 620 4594
Fragments arranged by size:
4594 958 620
Acc65I G'GTAC_C
Cuts at: 3493 3493
Size: 6172
AclI AA'CG_TT
Cuts at: 1563 4299 4672 1563
Size: 2736 373 3063
Fragments arranged by size:
3063 2736 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3410 4235 5619 5887 1632
Size: 1778 825 1384 268 1917
Fragments arranged by size:
1917 1778 1384 825 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5975
AflIII A'CryG_T
Cuts at: 1203 5071 6048 1203
Size: 3868 977 1327
Fragments arranged by size:
3868 1327 977
AgeI A'CCGG_T
Cuts at: 5003 5003
Size: 6172
AhdI GACnn_n'nnGTC
Cuts at: 2944 3282 4901 2944
Size: 338 1619 4215
Fragments arranged by size:
4215 1619 338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6172
AlwNI CAG_nnn'CTG
Cuts at: 2255 3433 3915 5487 2255
Size: 1178 482 1572 2940
Fragments arranged by size:
2940 1572 1178 482
ApaLI G'TGCA_C
Cuts at: 4229 5385 4229
Size: 1156 5016
ApoI r'AATT_y
Cuts at: 1493 3481 3707 3859 5968 1493
Size: 1988 226 152 2109 1697
Fragments arranged by size:
2109 1988 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4726 160
Size: 872 3694 1606
Fragments arranged by size:
3694 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 3348 3464 3497 1870
Size: 73 69 1336 116 33 4545
Fragments arranged by size:
4545 1336 116 73 69 33
BamHI G'GATC_C
Cuts at: 2916 3502 3869 2916
Size: 586 367 5219
Fragments arranged by size:
5219 586 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2543 3493 4948 618
Size: 325 217 627 756 950 1455 1842
Fragments arranged by size:
1842 1455 950 756 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 3491 1391
Size: 622 1478 4072
Fragments arranged by size:
4072 1478 622
BbeI G_GCGC'C
Cuts at: 2547 2547
Size: 6172
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5201
BbvCI CC'TCA_GC
Cuts at: 2374 2374
Size: 6172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3326 3360 4363 4397 1903
Size: 34 1389 34 1003 34 3678
Fragments arranged by size:
3678 1389 1003 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4064 5274 4064
Size: 1210 4962
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6172
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2432 3450 3467
Size: 107 122 71 631 605 760 1018 17
Cuts at: 3467 4783 136
Size: 1316 1525
Fragments arranged by size:
1525 1316 1018 760 631 605 122 107
71 17
BglII A'GATC_T
Cuts at: 2550 2784 2826 6167 2550
Size: 234 42 3341 2555
Fragments arranged by size:
3341 2555 234 42
BlpI GC'TnA_GC
Cuts at: 3440 3589 3440
Size: 149 6023
Bme1580I G_kGCm'C
Cuts at: 1790 2330 4233 5389 1790
Size: 540 1903 1156 2573
Fragments arranged by size:
2573 1903 1156 540
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6172
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3903 4861 442
Size: 416 53 83 399 24 2486 958 1753
Fragments arranged by size:
2486 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2068 2291 2371 2654 2839 3113 3308
Size: 800 29 223 80 283 185 274 195
Cuts at: 3308 4832 1239
Size: 1524 2579
Fragments arranged by size:
2579 1524 800 283 274 223 195 185
80 29
Bpu10I CC'TnA_GC
Cuts at: 2374 2374
Size: 6172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2197 2289 2972 4297 5162 5460 2197
Size: 92 683 1325 865 298 2909
Fragments arranged by size:
2909 1325 865 683 298 92
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2807 2863 3377 3412 4835 882
Size: 617 1308 56 514 35 1423 2219
Fragments arranged by size:
2219 1423 1308 617 514 56 35
BsaAI yAC'GTr
Cuts at: 493 1204 3963 493
Size: 711 2759 2702
Fragments arranged by size:
2759 2702 711
BsaBI GATnn'nnATC
Cuts at: 6166 6166
Size: 6172
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2544 3979 4361 4996
Size: 53 83 186 936 1011 1435 382 635
Cuts at: 4996 275
Size: 1451
Fragments arranged by size:
1451 1435 1011 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2913 4605 5003 5277 5424 5835 2913
Size: 1692 398 274 147 411 3250
Fragments arranged by size:
3250 1692 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5947
Fragments arranged by size:
5947 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6172
BseYI C'CCAG_C
Cuts at: 2422 2512 2517 3222 3387 5375 5871 2422
Size: 90 5 705 165 1988 496 2723
Fragments arranged by size:
2723 1988 705 496 165 90 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2981 3099 3132 1163
Size: 573 1245 118 33 4203
Fragments arranged by size:
4203 1245 573 118 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3622 4383 5411 1540
Size: 275 186 1621 761 1028 2301
Fragments arranged by size:
2301 1621 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2577 3441 4233 4318 5389 2025
Size: 552 864 792 85 1071 2808
Fragments arranged by size:
2808 1071 864 792 552 85
BsmI GAATG_Cn'
Cuts at: 3683 3776 3683
Size: 93 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2025 2330 2577 3441 3491 4233
Size: 399 223 12 305 247 864 50 742
Cuts at: 4233 4318 5389 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 864 742 399 305 247 223
85 50 12
BspEI T'CCGG_A
Cuts at: 5835 5835
Size: 6172
BspHI T'CATG_A
Cuts at: 4061 5791 4061
Size: 1730 4442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2588 2841 3346 3527 844
Size: 1744 253 505 181 3489
Fragments arranged by size:
3489 1744 505 253 181
BsrBI CCG'CTC
Cuts at: 1394 1823 4059 1394
Size: 429 2236 3507
Fragments arranged by size:
3507 2236 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4667 4841 65
Size: 1183 3419 174 1396
Fragments arranged by size:
3419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3135 4816 5003 1700
Size: 110 1325 1681 187 2869
Fragments arranged by size:
2869 1681 1325 187 110
BsrGI T'GTAC_A
Cuts at: 96 2366 96
Size: 2270 3902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6172
BssSI C'ACGA_G
Cuts at: 3314 4232 5244 3314
Size: 918 1012 4242
Fragments arranged by size:
4242 1012 918
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2029 794
Size: 1235 4937
BstBI TT'CG_AA
Cuts at: 4976 4976
Size: 6172
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6172
BstXI CCAn_nnnn'nTGG
Cuts at: 2269 2299 2343 2269
Size: 30 44 6098
Fragments arranged by size:
6098 44 30
BstYI r'GATC_y
Cuts at: 1972 2550 2784 2826 2916 3502 3869 4254 4271
Size: 578 234 42 90 586 367 385 17
Cuts at: 4271 5723 5809 5821 6167 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 586 578 385 367 346 234
90 86 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 3266 3266
Size: 6172
BtgI C'CryG_G
Cuts at: 513 1065 2004 2152 2412 2592 513
Size: 552 939 148 260 180 4093
Fragments arranged by size:
4093 939 552 260 180 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2741 2906 487
Size: 683 56 1515 165 3753
Fragments arranged by size:
3753 1515 683 165 56
BtsI GCAGTG_nn'
Cuts at: 2027 3149 3688 4482 4502 2027
Size: 1122 539 794 20 3697
Fragments arranged by size:
3697 1122 794 539 20
ClaI AT'CG_AT
Cuts at: 1554 3456 1554
Size: 1902 4270
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3151 3186 529
Size: 35 2587 35 3515
Fragments arranged by size:
3515 2587 35 35
DraI TTT'AAA
Cuts at: 3476 3476
Size: 6172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5179 809
Size: 507 3863 1802
Fragments arranged by size:
3863 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2114 2409 2664
Size: 54 1425 50 275 18 284 295 255
Cuts at: 2664 3619 4508 6137 8
Size: 955 889 1629 43
Fragments arranged by size:
1629 1425 955 889 295 284 275 255
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3619 1537
Size: 275 1807 4090
Fragments arranged by size:
4090 1807 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 4102 1353
Size: 1107 1642 3423
Fragments arranged by size:
3423 1642 1107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4759 5133 5279 387
Size: 1064 138 3170 374 146 1280
Fragments arranged by size:
3170 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2068 2291 2371 2654 2839 3113
Size: 393 407 29 223 80 283 185 274
Cuts at: 3113 3308 3410 4235 4832 5619 5887 1239
Size: 195 102 825 597 787 268 1524
Fragments arranged by size:
1524 825 787 597 407 393 283 274
268 223 195 185 102 80 29
EcoO109I rG'GnC_Cy
Cuts at: 2037 2234 2497 2769 2037
Size: 197 263 272 5440
Fragments arranged by size:
5440 272 263 197
EcoRI G'AATT_C
Cuts at: 3481 3859 3481
Size: 378 5794
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2665 2668 2728 2665
Size: 3 60 6109
Fragments arranged by size:
6109 60 3
FspI TGC'GCA
Cuts at: 2033 4678 2033
Size: 2645 3527
HaeII r_GCGC'y
Cuts at: 1678 2547 5319 1678
Size: 869 2772 2531
Fragments arranged by size:
2772 2531 869
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2930 2962 3268 3300 3376
Size: 32 135 32 1358 32 306 32 76
Cuts at: 3376 3408 4813 4845 4887 4919 1373
Size: 32 1405 32 42 32 2626
Fragments arranged by size:
2626 1405 1358 306 135 76 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3163 3516 3762 669
Size: 988 281 1225 353 246 3079
Fragments arranged by size:
3079 1225 988 353 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6172
HpaI GTT'AAC
Cuts at: 3762 3762
Size: 6172
KasI G'GCGC_C
Cuts at: 2543 2543
Size: 6172
KpnI G_GTAC'C
Cuts at: 3497 3497
Size: 6172
MfeI C'AATT_G
Cuts at: 3771 3771
Size: 6172
MluI A'CGCG_T
Cuts at: 6048 6048
Size: 6172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3441 5286 5470 1305
Size: 2136 1845 184 2007
Fragments arranged by size:
2136 2007 1845 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2116 2411 2666 10
Size: 54 1425 343 284 295 255 3516
Fragments arranged by size:
3516 1425 343 295 284 255 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2747 3128 3251 4130 4489 4648 518
Size: 648 1581 381 123 879 359 159 2042
Fragments arranged by size:
2042 1581 879 648 381 359 159 123
MspA1I CmG'CkG
Cuts at: 1606 2126 2252 2378 3588 4265 5413 5658 1606
Size: 520 126 126 1210 677 1148 245 2120
Fragments arranged by size:
2120 1210 1148 677 520 245 126 126
NarI GG'CG_CC
Cuts at: 2544 2544
Size: 6172
NcoI C'CATG_G
Cuts at: 513 1065 2004 2592 513
Size: 552 939 588 4093
Fragments arranged by size:
4093 939 588 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6172
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6172
NotI GC'GGCC_GC
Cuts at: 3619 3619
Size: 6172
NspI r_CATG'y
Cuts at: 1132 1229 2560 3530 5075 1132
Size: 97 1331 970 1545 2229
Fragments arranged by size:
2229 1545 1331 970 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6172
PciI A'CATG_T
Cuts at: 5071 5071
Size: 6172
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3055 1108
Size: 1947 4225
PfoI T'CCnGG_A
Cuts at: 2045 2045
Size: 6172
PmeI GTTT'AAAC
Cuts at: 3476 3476
Size: 6172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 5754 5786 4241
Size: 32 1481 32 4627
Fragments arranged by size:
4627 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2037 2234 2769 2037
Size: 197 535 5440
Fragments arranged by size:
5440 535 197
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6172
PsiI TTA'TAA
Cuts at: 3742 3742
Size: 6172
PstI C_TGCA'G
Cuts at: 830 2383 3524 830
Size: 1553 1141 3478
Fragments arranged by size:
3478 1553 1141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6172
PvuII CAG'CTG
Cuts at: 2126 2252 2378 2126
Size: 126 126 5920
Fragments arranged by size:
5920 126 126
SalI G'TCGA_C
Cuts at: 1936 3514 1936
Size: 1578 4594
SbfI CC_TGCA'GG
Cuts at: 3524 3524
Size: 6172
ScaI AGT'ACT
Cuts at: 4420 6132 4420
Size: 1712 4460
SexAI A'CCwGG_T
Cuts at: 2218 2218
Size: 6172
SfcI C'TryA_G
Cuts at: 826 1045 2379 2764 3520 4655 5336 5527 826
Size: 219 1334 385 756 1135 681 191 1471
Fragments arranged by size:
1471 1334 1135 756 681 385 219 191
SfoI GGC'GCC
Cuts at: 2545 2545
Size: 6172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6172
SmaI CCC'GGG
Cuts at: 1872 2014 3499 1872
Size: 142 1485 4545
Fragments arranged by size:
4545 1485 142
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2176 2304 2987 4276 5177 5439
Size: 197 926 233 128 683 1289 901 262
Cuts at: 5439 820
Size: 1553
Fragments arranged by size:
1553 1289 926 901 683 262 233 197
128
SnaBI TAC'GTA
Cuts at: 493 3963 493
Size: 3470 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6172
SphI G_CATG'C
Cuts at: 1132 3530 1132
Size: 2398 3774
SrfI GCCC'GGGC
Cuts at: 2014 2014
Size: 6172
SspI AAT'ATT
Cuts at: 5 52 4096 5
Size: 47 4044 2081
Fragments arranged by size:
4044 2081 47
StuI AGG'CCT
Cuts at: 1517 2435 2457 2473 2648 1517
Size: 918 22 16 175 5041
Fragments arranged by size:
5041 918 175 22 16
StyI C'CwwG_G
Cuts at: 513 1065 2004 2056 2298 2592 3611 513
Size: 552 939 52 242 294 1019 3074
Fragments arranged by size:
3074 1019 939 552 294 242 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3118 4199 4216 4369 4554 4999 325
Size: 1255 1538 1081 17 153 185 445 1498
Fragments arranged by size:
1538 1498 1255 1081 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2366 4418 6130
Size: 274 80 33 51 1443 389 2052 1712
Cuts at: 6130 96
Size: 138
Fragments arranged by size:
2052 1712 1443 389 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6132 6164 6132
Size: 32 6140
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6172
XbaI T'CTAG_A
Cuts at: 3508 3508
Size: 6172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2340 2340
Size: 6172
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6172
XmaI C'CCGG_G
Cuts at: 1870 2012 3497 1870
Size: 142 1485 4545
Fragments arranged by size:
4545 1485 142
XmnI GAAnn'nnTTC
Cuts at: 2648 4301 2648
Size: 1653 4519
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3980 276
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SwaI