Restriction map for
pFN21AE5657
(Circular) (Six-base) MAPSORT of: pFN21AE5657.seq Check: 2410 from: 1 to: 6154
pFN21AE5657 6154 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3964 278
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3497 1937
Size: 1560 4594
Acc65I G'GTAC_C
Cuts at: 3475 3475
Size: 6154
AclI AA'CG_TT
Cuts at: 1563 2968 4281 4654 1563
Size: 1405 1313 373 3063
Fragments arranged by size:
3063 1405 1313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2588 4217 5601 5869 1632
Size: 956 1629 1384 268 1917
Fragments arranged by size:
1917 1629 1384 956 268
AfeI AGC'GCT
Cuts at: 2134 2134
Size: 6154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5957
AflIII A'CryG_T
Cuts at: 1203 5053 6030 1203
Size: 3850 977 1327
Fragments arranged by size:
3850 1327 977
AgeI A'CCGG_T
Cuts at: 4985 4985
Size: 6154
AhdI GACnn_n'nnGTC
Cuts at: 4883 4883
Size: 6154
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6154
AlwNI CAG_nnn'CTG
Cuts at: 2287 2566 3897 5469 2287
Size: 279 1331 1572 2972
Fragments arranged by size:
2972 1572 1331 279
ApaI G_GGCC'C
Cuts at: 2048 2061 2048
Size: 13 6141
ApaLI G'TGCA_C
Cuts at: 4211 5367 4211
Size: 1156 4998
ApoI r'AATT_y
Cuts at: 1493 3463 3689 3841 5950 1493
Size: 1970 226 152 2109 1697
Fragments arranged by size:
2109 1970 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 2128 3479 1870
Size: 73 185 1351 4545
Fragments arranged by size:
4545 1351 185 73
AvrII C'CTAG_G
Cuts at: 2170 3116 2170
Size: 946 5208
BamHI G'GATC_C
Cuts at: 3484 3851 3484
Size: 367 5787
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2380 3475 4930 618
Size: 325 217 627 309 284 1095 1455 1842
Fragments arranged by size:
1842 1455 1095 627 325 309 284 217
BanII G_rGCy'C
Cuts at: 1391 2048 2061 2485 3473 1391
Size: 657 13 424 988 4072
Fragments arranged by size:
4072 988 657 424 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2396 2958 3441 928
Size: 971 497 562 483 3641
Fragments arranged by size:
3641 971 562 497 483
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4345 4379 1903
Size: 34 2408 34 3678
Fragments arranged by size:
3678 2408 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2635 4046 5256 2635
Size: 1411 1210 3533
Fragments arranged by size:
3533 1411 1210
BclI T'GATC_A
Cuts at: 1548 3421 1548
Size: 1873 4281
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 4765 136
Size: 107 122 71 631 605 380 2713 1525
Fragments arranged by size:
2713 1525 631 605 380 122 107 71
BglII A'GATC_T
Cuts at: 2885 3062 6149 2885
Size: 177 3087 2890
Fragments arranged by size:
3087 2890 177
BlpI GC'TnA_GC
Cuts at: 3571 3571
Size: 6154
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2061 4215 5371 1790
Size: 258 13 2154 1156 2573
Fragments arranged by size:
2573 2154 1156 258 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3885 4843 442
Size: 416 53 83 399 24 2468 958 1753
Fragments arranged by size:
2468 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2139 1056
Size: 1083 5071
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2799 2904 2799
Size: 105 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2544 4814 1239
Size: 1305 2270 2579
Fragments arranged by size:
2579 2270 1305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4279 5144 5442 4279
Size: 865 298 4991
Fragments arranged by size:
4991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2621 3087 4817 882
Size: 617 1122 466 1730 2219
Fragments arranged by size:
2219 1730 1122 617 466
BsaAI yAC'GTr
Cuts at: 493 1204 3945 493
Size: 711 2741 2702
Fragments arranged by size:
2741 2702 711
BsaBI GATnn'nnATC
Cuts at: 2758 3423 6148 2758
Size: 665 2725 2764
Fragments arranged by size:
2764 2725 665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3961 4343 4978 275
Size: 53 83 186 936 2428 382 635 1451
Fragments arranged by size:
2428 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4587 4985 5259 5406 5817 4587
Size: 398 274 147 411 4924
Fragments arranged by size:
4924 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3049 3079 1178
Size: 30 165 30 1646 30 4253
Fragments arranged by size:
4253 1646 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2788 2791 1186
Size: 1602 3 4549
Fragments arranged by size:
4549 1602 3
BseYI C'CCAG_C
Cuts at: 2027 2048 5357 5853 2027
Size: 21 3309 496 2328
Fragments arranged by size:
3309 2328 496 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2493 1163
Size: 573 757 4824
Fragments arranged by size:
4824 757 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3604 4365 5393 1540
Size: 275 186 1603 761 1028 2301
Fragments arranged by size:
2301 1603 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2924 4215 4300 5371 2924
Size: 1291 85 1071 3707
Fragments arranged by size:
3707 1291 1071 85
BsmI GAATG_Cn'
Cuts at: 2746 3665 3758 2746
Size: 919 93 5142
Fragments arranged by size:
5142 919 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2818 3025 2818
Size: 207 5947
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2061 2485 2924 3473 4215 4300
Size: 399 258 13 424 439 549 742 85
Cuts at: 4300 5371 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 549 439 424 399 258
85 13
BspEI T'CCGG_A
Cuts at: 5817 5817
Size: 6154
BspHI T'CATG_A
Cuts at: 3427 4043 5773 3427
Size: 616 1730 3808
Fragments arranged by size:
3808 1730 616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2027 2390 3509 844
Size: 1183 363 1119 3489
Fragments arranged by size:
3489 1183 1119 363
BsrBI CCG'CTC
Cuts at: 1394 1823 3105 4041 1394
Size: 429 1282 936 3507
Fragments arranged by size:
3507 1282 936 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4649 4823 65
Size: 1183 3401 174 1396
Fragments arranged by size:
3401 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4798 4985 1700
Size: 110 2988 187 2869
Fragments arranged by size:
2988 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6154
BssSI C'ACGA_G
Cuts at: 2232 4214 5226 2232
Size: 1982 1012 3160
Fragments arranged by size:
3160 1982 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6154
BstBI TT'CG_AA
Cuts at: 4958 4958
Size: 6154
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6154
BstYI r'GATC_y
Cuts at: 1972 2885 3062 3484 3851 4236 4253 5705 5791
Size: 913 177 422 367 385 17 1452 86
Cuts at: 5791 5803 6149 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 913 422 385 367 346 177
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5602
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5415
Fragments arranged by size:
5415 683 56
BtsI GCAGTG_nn'
Cuts at: 2031 2400 3670 4464 4484 2031
Size: 369 1270 794 20 3701
Fragments arranged by size:
3701 1270 794 369 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6119
DraI TTT'AAA
Cuts at: 3458 3458
Size: 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5161 809
Size: 507 3845 1802
Fragments arranged by size:
3845 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3601 4490 6119
Size: 54 1425 50 275 18 1771 889 1629
Cuts at: 6119 8
Size: 43
Fragments arranged by size:
1771 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3601 1537
Size: 275 1789 4090
Fragments arranged by size:
4090 1789 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2201 3197 3276 3315 4084 1353
Size: 812 36 996 79 39 769 3423
Fragments arranged by size:
3423 996 812 769 79 39 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2082 2100 2893 4741 5115 5261
Size: 1064 138 493 18 793 1848 374 146
Cuts at: 5261 387
Size: 1280
Fragments arranged by size:
1848 1280 1064 793 493 374 146 138
18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2544 2588 4217 4814 5601 5869 1239
Size: 393 912 44 1629 597 787 268 1524
Fragments arranged by size:
1629 1524 912 787 597 393 268 44
EcoO109I rG'GnC_Cy
Cuts at: 2045 2045
Size: 6154
EcoRI G'AATT_C
Cuts at: 3463 3841 3463
Size: 378 5776
FspI TGC'GCA
Cuts at: 4660 4660
Size: 6154
HaeII r_GCGC'y
Cuts at: 1678 2136 2460 5301 1678
Size: 458 324 2841 2531
Fragments arranged by size:
2841 2531 458 324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2140 2172 2249 2281 4795
Size: 32 135 32 568 32 77 32 2514
Cuts at: 4795 4827 4869 4901 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2514 568 135 77 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 3498 3744 669
Size: 988 281 370 1190 246 3079
Fragments arranged by size:
3079 1190 988 370 281 246
HindIII A'AGCT_T
Cuts at: 748 2364 2570 748
Size: 1616 206 4332
Fragments arranged by size:
4332 1616 206
HpaI GTT'AAC
Cuts at: 3744 3744
Size: 6154
KpnI G_GTAC'C
Cuts at: 3479 3479
Size: 6154
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5268 5452 1305
Size: 3963 184 2007
Fragments arranged by size:
3963 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4332
Fragments arranged by size:
4332 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3443 4112 4471 4630 518
Size: 648 2277 669 359 159 2042
Fragments arranged by size:
2277 2042 669 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 3570 4247 5395 5640 1606
Size: 446 1518 677 1148 245 2120
Fragments arranged by size:
2120 1518 1148 677 446 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5602
NdeI CA'TA_TG
Cuts at: 387 2332 387
Size: 1945 4209
NheI G'CTAG_C
Cuts at: 1052 2135 1052
Size: 1083 5071
NotI GC'GGCC_GC
Cuts at: 3601 3601
Size: 6154
NspI r_CATG'y
Cuts at: 1132 1229 3512 5057 1132
Size: 97 2283 1545 2229
Fragments arranged by size:
2283 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6154
PciI A'CATG_T
Cuts at: 5053 5053
Size: 6154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6154
PfoI T'CCnGG_A
Cuts at: 2521 2521
Size: 6154
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2178 2210 4223 4255 5736 5768 2178
Size: 32 2013 32 1481 32 2564
Fragments arranged by size:
2564 2013 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2120 2830 1613
Size: 507 710 4937
Fragments arranged by size:
4937 710 507
PsiI TTA'TAA
Cuts at: 3724 3724
Size: 6154
PspOMI G'GGCC_C
Cuts at: 2044 2057 2044
Size: 13 6141
PstI C_TGCA'G
Cuts at: 830 2038 2557 3506 830
Size: 1208 519 949 3478
Fragments arranged by size:
3478 1208 949 519
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
SalI G'TCGA_C
Cuts at: 1936 3496 1936
Size: 1560 4594
SbfI CC_TGCA'GG
Cuts at: 3506 3506
Size: 6154
ScaI AGT'ACT
Cuts at: 2581 4402 6114 2581
Size: 1821 1712 2621
Fragments arranged by size:
2621 1821 1712
SfcI C'TryA_G
Cuts at: 826 1045 2034 2553 2712 3502 4637 5318 5509
Size: 219 989 519 159 790 1135 681 191
Cuts at: 5509 826
Size: 1471
Fragments arranged by size:
1471 1135 989 790 681 519 219 191
159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6154
SmaI CCC'GGG
Cuts at: 1872 3481 1872
Size: 1609 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4258 5159 5421 820
Size: 197 926 2315 901 262 1553
Fragments arranged by size:
2315 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6154
SphI G_CATG'C
Cuts at: 1132 3512 1132
Size: 2380 3774
SspI AAT'ATT
Cuts at: 5 52 4078 5
Size: 47 4026 2081
Fragments arranged by size:
4026 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6154
StyI C'CwwG_G
Cuts at: 513 1065 2170 2873 3116 3593 513
Size: 552 1105 703 243 477 3074
Fragments arranged by size:
3074 1105 703 552 477 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3069 4181 4198 4351 4536 4981 325
Size: 1255 1489 1112 17 153 185 445 1498
Fragments arranged by size:
1498 1489 1255 1112 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2579 4400 6112
Size: 274 80 33 51 1443 602 1821 1712
Cuts at: 6112 96
Size: 138
Fragments arranged by size:
1821 1712 1443 602 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2023 2055 3049 3081 6114 6146 2023
Size: 32 994 32 3033 32 2031
Fragments arranged by size:
3033 2031 994 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6154
XbaI T'CTAG_A
Cuts at: 2795 3011 3490 2795
Size: 216 479 5459
Fragments arranged by size:
5459 479 216
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6154
XmaI C'CCGG_G
Cuts at: 1870 3479 1870
Size: 1609 4545
XmnI GAAnn'nnTTC
Cuts at: 2746 4283 2746
Size: 1537 4617
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3962 276
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI