Restriction map for
pFN21AE5654
(Circular) (Six-base) MAPSORT of: pFN21AE5654.seq Check: 2382 from: 1 to: 6334
pFN21AE5654 6334 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2118 4144 278
Size: 53 83 186 936 582 2026 2468
Fragments arranged by size:
2468 2026 936 582 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3303 3677 1937
Size: 1366 374 4594
Fragments arranged by size:
4594 1366 374
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2861 2873 4397 5781 6049 1632
Size: 1229 12 1524 1384 268 1917
Fragments arranged by size:
1917 1524 1384 1229 268 12
AflII C'TTAA_G
Cuts at: 820 1017 2693 820
Size: 197 1676 4461
Fragments arranged by size:
4461 1676 197
AflIII A'CryG_T
Cuts at: 1203 5233 6210 1203
Size: 4030 977 1327
Fragments arranged by size:
4030 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 5063 5063
Size: 6334
AleI CACnn'nnGTG
Cuts at: 1166 3254 1166
Size: 2088 4246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6334
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 2198
Size: 32 6302
AlwNI CAG_nnn'CTG
Cuts at: 4077 5649 4077
Size: 1572 4762
ApaLI G'TGCA_C
Cuts at: 4391 5547 4391
Size: 1156 5178
ApoI r'AATT_y
Cuts at: 1493 3643 3869 4021 6130 1493
Size: 2150 226 152 2109 1697
Fragments arranged by size:
2150 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 3659 1870
Size: 73 1716 4545
Fragments arranged by size:
4545 1716 73
BamHI G'GATC_C
Cuts at: 2393 3664 4031 2393
Size: 1271 367 4696
Fragments arranged by size:
4696 1271 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2291 3248 3655 5110
Size: 325 217 627 297 207 957 407 1455
Cuts at: 5110 618
Size: 1842
Fragments arranged by size:
1842 1455 957 627 407 325 297 217
207
BanII G_rGCy'C
Cuts at: 1391 2165 3653 1391
Size: 774 1488 4072
Fragments arranged by size:
4072 1488 774
BbeI G_GCGC'C
Cuts at: 2088 2088
Size: 6334
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5363
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3062 3096 4525 4559 1903
Size: 34 50 34 1041 34 1429 34 3678
Fragments arranged by size:
3678 1429 1041 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4226 5436 4226
Size: 1210 5124
BclI T'GATC_A
Cuts at: 1548 3517 1548
Size: 1969 4365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2198 4945 136
Size: 107 122 71 631 605 526 2747 1525
Fragments arranged by size:
2747 1525 631 605 526 122 107 71
BglII A'GATC_T
Cuts at: 6329 6329
Size: 6334
BlpI GC'TnA_GC
Cuts at: 3751 3751
Size: 6334
Bme1580I G_kGCm'C
Cuts at: 1790 3251 4395 5551 1790
Size: 1461 1144 1156 2573
Fragments arranged by size:
2573 1461 1156 1144
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3578 4065 5023
Size: 416 53 83 399 24 2161 487 958
Cuts at: 5023 442
Size: 1753
Fragments arranged by size:
2161 1753 958 487 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4994 1239
Size: 3755 2579
Bpu10I CC'TnA_GC
Cuts at: 2542 2542
Size: 6334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2349 2485 4459 5324 5622 2349
Size: 136 1974 865 298 3061
Fragments arranged by size:
3061 1974 865 298 136
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4997 882
Size: 617 3498 2219
Fragments arranged by size:
3498 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4125 493
Size: 711 2921 2702
Fragments arranged by size:
2921 2702 711
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2115 2190 4141
Size: 53 83 186 936 552 30 75 1951
Cuts at: 4141 4523 5158 275
Size: 382 635 1451
Fragments arranged by size:
1951 1451 936 635 552 382 186 83
75 53 30
BsaWI w'CCGG_w
Cuts at: 4767 5165 5439 5586 5997 4767
Size: 398 274 147 411 5104
Fragments arranged by size:
5104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 1178
Size: 30 165 30 1002 30 5077
Fragments arranged by size:
5077 1002 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2429 1186
Size: 1243 5091
BseYI C'CCAG_C
Cuts at: 2065 5537 6033 2065
Size: 3472 496 2366
Fragments arranged by size:
3472 2366 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 1163
Size: 573 399 5362
Fragments arranged by size:
5362 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3784 4545 5573 1540
Size: 275 186 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3002 4395 4480 5551 3002
Size: 1393 85 1071 3785
Fragments arranged by size:
3785 1393 1071 85
BsmI GAATG_Cn'
Cuts at: 3845 3938 3845
Size: 93 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 3002 3251 3653 4395 4480 5551
Size: 399 375 837 249 402 742 85 1071
Cuts at: 5551 1391
Size: 2174
Fragments arranged by size:
2174 1071 837 742 402 399 375 249
85
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 4223 5953 4223
Size: 1730 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2107 3689 844
Size: 1263 1582 3489
Fragments arranged by size:
3489 1582 1263
BsrBI CCG'CTC
Cuts at: 1394 1823 4221 1394
Size: 429 2398 3507
Fragments arranged by size:
3507 2398 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2680 3310 4829 5003 65
Size: 1183 1432 630 1519 174 1396
Fragments arranged by size:
1519 1432 1396 1183 630 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4978 5165 1700
Size: 110 3168 187 2869
Fragments arranged by size:
3168 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6334
BssSI C'ACGA_G
Cuts at: 4394 5406 4394
Size: 1012 5322
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6334
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 3399 1184
Size: 2215 4119
BstXI CCAn_nnnn'nTGG
Cuts at: 3491 3491
Size: 6334
BstYI r'GATC_y
Cuts at: 1972 2393 3664 4031 4416 4433 5885 5971 5983
Size: 421 1271 367 385 17 1452 86 12
Cuts at: 5983 6329 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1271 421 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3304 3304
Size: 6334
Bsu36I CC'TnA_GG
Cuts at: 2279 2279
Size: 6334
BtgI C'CryG_G
Cuts at: 513 1065 2202 2210 513
Size: 552 1137 8 4637
Fragments arranged by size:
4637 1137 552 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5595
Fragments arranged by size:
5595 683 56
BtsI GCAGTG_nn'
Cuts at: 2603 3850 4644 4664 2603
Size: 1247 794 20 4273
Fragments arranged by size:
4273 1247 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DraIII CAC_nnn'GTG
Cuts at: 2158 2158
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 809
Size: 507 4025 1802
Fragments arranged by size:
4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2253 3554
Size: 54 1425 50 275 18 369 54 1301
Cuts at: 3554 3781 4670 6299 8
Size: 227 889 1629 43
Fragments arranged by size:
1629 1425 1301 889 369 275 227 54
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2337 2644 2837 3231 4264 1353
Size: 984 307 193 394 1033 3423
Fragments arranged by size:
3423 1033 984 394 307 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3069 4921 5295 5441 387
Size: 1064 138 1480 1852 374 146 1280
Fragments arranged by size:
1852 1480 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2861 2873 4397 4994 5781 6049 1239
Size: 393 1229 12 1524 597 787 268 1524
Fragments arranged by size:
1524 1524 1229 787 597 393 268 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2696 2696
Size: 6334
EcoO109I rG'GnC_Cy
Cuts at: 2069 3455 2069
Size: 1386 4948
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2320 2791 2320
Size: 471 5863
FspI TGC'GCA
Cuts at: 2103 4840 2103
Size: 2737 3597
HaeII r_GCGC'y
Cuts at: 1678 2088 3062 5481 1678
Size: 410 974 2419 2531
Fragments arranged by size:
2531 2419 974 410
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2621 2653 4975 5007 5049
Size: 32 135 32 1049 32 2322 32 42
Cuts at: 5049 5081 1373
Size: 32 2626
Fragments arranged by size:
2626 2322 1049 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2371 3678 3924 669
Size: 988 281 433 1307 246 3079
Fragments arranged by size:
3079 1307 988 433 281 246
HindIII A'AGCT_T
Cuts at: 748 2240 3293 748
Size: 1492 1053 3789
Fragments arranged by size:
3789 1492 1053
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KasI G'GCGC_C
Cuts at: 2084 2084
Size: 6334
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 2450 2747 3933 2450
Size: 297 1186 4851
Fragments arranged by size:
4851 1186 297
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2409 3096 3508 5448 5632 1305
Size: 1104 687 412 1940 184 2007
Fragments arranged by size:
2007 1940 1104 687 412 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2255 10
Size: 54 1425 343 369 54 4089
Fragments arranged by size:
4089 1425 369 343 54 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3254 4292 4651 4810 518
Size: 648 2088 1038 359 159 2042
Fragments arranged by size:
2088 2042 1038 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2065 2149 2212 3086 3750 4427 5575
Size: 435 24 84 63 874 664 677 1148
Cuts at: 5575 5820 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 874 677 664 435 245 84
63 24
NarI GG'CG_CC
Cuts at: 2085 2085
Size: 6334
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5782
NdeI CA'TA_TG
Cuts at: 387 3046 387
Size: 2659 3675
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 3692 5237 1132
Size: 97 2463 1545 2229
Fragments arranged by size:
2463 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6334
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3184 1108
Size: 2076 4258
PfoI T'CCnGG_A
Cuts at: 2625 2625
Size: 6334
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5916 5948 4403
Size: 32 1481 32 4789
Fragments arranged by size:
4789 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2069 3455 2069
Size: 1386 4948
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2480 2512 2480
Size: 32 6302
PstI C_TGCA'G
Cuts at: 830 3686 830
Size: 2856 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 3086 3086
Size: 6334
SacII CC_GC'GG
Cuts at: 2213 2213
Size: 6334
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SanDI GG'GwC_CC
Cuts at: 2069 2069
Size: 6334
SapI GCTCTTCn'nnn_
Cuts at: 2644 2837 2644
Size: 193 6141
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 3392 4582 6294 3392
Size: 1190 1712 3432
Fragments arranged by size:
3432 1712 1190
SexAI A'CCwGG_T
Cuts at: 2154 2154
Size: 6334
SfcI C'TryA_G
Cuts at: 826 1045 2511 3682 4817 5498 5689 826
Size: 219 1466 1171 1135 681 191 1471
Fragments arranged by size:
1471 1466 1171 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2086 2086
Size: 6334
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 3661 1872
Size: 1789 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2364 2500 2693 4438 5339 5601
Size: 197 926 421 136 193 1745 901 262
Cuts at: 5601 820
Size: 1553
Fragments arranged by size:
1745 1553 926 901 421 262 197 193
136
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 3692 1132
Size: 2560 3774
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 3007 1517
Size: 1490 4844
StyI C'CwwG_G
Cuts at: 513 1065 2250 2643 3252 3332 3773 513
Size: 552 1185 393 609 80 441 3074
Fragments arranged by size:
3074 1185 609 552 441 393 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 4361 4378 4531 4716 5161 325
Size: 1255 573 2208 17 153 185 445 1498
Fragments arranged by size:
2208 1498 1255 573 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3390 4580 6292
Size: 274 80 33 51 1443 1413 1190 1712
Cuts at: 6292 96
Size: 138
Fragments arranged by size:
1712 1443 1413 1190 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3123 3155 3313 3345 6294 6326 3123
Size: 32 158 32 2949 32 3131
Fragments arranged by size:
3131 2949 158 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3259 3259
Size: 6334
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6334
XmaI C'CCGG_G
Cuts at: 1870 3659 1870
Size: 1789 4545
XmnI GAAnn'nnTTC
Cuts at: 2480 2846 2931 4463 2480
Size: 366 85 1532 4351
Fragments arranged by size:
4351 1532 366 85
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2116 4142 276
Size: 53 83 186 936 582 2026 2468
Fragments arranged by size:
2468 2026 936 582 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacI SfiI SrfI SwaI