Restriction map for
pFN21AE5651
(Circular) (Six-base) MAPSORT of: pFN21AE5651.seq Check: 8090 from: 1 to: 6274
pFN21AE5651 6274 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2698 2698
Size: 6274
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4084 278
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3617 1937
Size: 1680 4594
Acc65I G'GTAC_C
Cuts at: 3595 3595
Size: 6274
AclI AA'CG_TT
Cuts at: 1563 4401 4774 1563
Size: 2838 373 3063
Fragments arranged by size:
3063 2838 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3026 4337 5721 5989 1632
Size: 1394 1311 1384 268 1917
Fragments arranged by size:
1917 1394 1384 1311 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6077
AflIII A'CryG_T
Cuts at: 1203 3412 5173 6150 1203
Size: 2209 1761 977 1327
Fragments arranged by size:
2209 1761 1327 977
AgeI A'CCGG_T
Cuts at: 5105 5105
Size: 6274
AhdI GACnn_n'nnGTC
Cuts at: 5003 5003
Size: 6274
AleI CACnn'nnGTG
Cuts at: 1166 2861 1166
Size: 1695 4579
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3369 1184
Size: 2185 4089
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 6242
AlwNI CAG_nnn'CTG
Cuts at: 2183 2656 4017 5589 2183
Size: 473 1361 1572 2868
Fragments arranged by size:
2868 1572 1361 473
ApaI G_GGCC'C
Cuts at: 2344 2344
Size: 6274
ApaLI G'TGCA_C
Cuts at: 4331 5487 4331
Size: 1156 5118
ApoI r'AATT_y
Cuts at: 1493 3036 3583 3809 3961 6070 1493
Size: 1543 547 226 152 2109 1697
Fragments arranged by size:
2109 1697 1543 547 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4828 160
Size: 872 3796 1606
Fragments arranged by size:
3796 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2265 3285 3599 1870
Size: 73 95 227 1020 314 4545
Fragments arranged by size:
4545 1020 314 227 95 73
BamHI G'GATC_C
Cuts at: 2042 2525 3604 3971 2042
Size: 483 1079 367 4345
Fragments arranged by size:
4345 1079 483 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3595 5050 618
Size: 325 217 627 1808 1455 1842
Fragments arranged by size:
1842 1808 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2344 3220 3292 3593 1391
Size: 953 876 72 301 4072
Fragments arranged by size:
4072 953 876 301 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4465 4499 1903
Size: 34 2528 34 3678
Fragments arranged by size:
3678 2528 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4166 5376 4166
Size: 1210 5064
BclI T'GATC_A
Cuts at: 1548 3504 1548
Size: 1956 4318
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2339 3281 4885
Size: 107 122 71 631 605 667 942 1604
Cuts at: 4885 136
Size: 1525
Fragments arranged by size:
1604 1525 942 667 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6269 6269
Size: 6274
BlpI GC'TnA_GC
Cuts at: 2310 3691 2310
Size: 1381 4893
Bme1580I G_kGCm'C
Cuts at: 1790 2261 2344 4335 5491 1790
Size: 471 83 1991 1156 2573
Fragments arranged by size:
2573 1991 1156 471 83
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6274
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2551 2578 4005
Size: 416 53 83 399 24 1134 27 1427
Cuts at: 4005 4963 442
Size: 958 1753
Fragments arranged by size:
1753 1427 1134 958 416 399 83 53
27 24
BmtI G_CTAG'C
Cuts at: 1056 2948 1056
Size: 1892 4382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 6274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2427 2734 4934 1239
Size: 1188 307 2200 2579
Fragments arranged by size:
2579 2200 1188 307
Bpu10I CC'TnA_GC
Cuts at: 2952 2952
Size: 6274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4399 5264 5562 4399
Size: 865 298 5111
Fragments arranged by size:
5111 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4937 882
Size: 617 3438 2219
Fragments arranged by size:
3438 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4065 493
Size: 711 2861 2702
Fragments arranged by size:
2861 2702 711
BsaBI GATnn'nnATC
Cuts at: 3019 6268 3019
Size: 3249 3025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4081 4463 5098 275
Size: 53 83 186 936 2548 382 635 1451
Fragments arranged by size:
2548 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4707 5105 5379 5526 5937 4707
Size: 398 274 147 411 5044
Fragments arranged by size:
5044 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2879 2909 3399 3429 1178
Size: 30 165 30 1476 30 490 30 4023
Fragments arranged by size:
4023 1476 490 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2253 2900 1186
Size: 1067 647 4560
Fragments arranged by size:
4560 1067 647
BseYI C'CCAG_C
Cuts at: 2396 2617 3148 3514 5477 5973 2396
Size: 221 531 366 1963 496 2697
Fragments arranged by size:
2697 1963 531 496 366 221
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3365 1163
Size: 573 1629 4072
Fragments arranged by size:
4072 1629 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3724 4485 5513 1540
Size: 275 186 1723 761 1028 2301
Fragments arranged by size:
2301 1723 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3189 3220 4335 4420 5491 3189
Size: 31 1115 85 1071 3972
Fragments arranged by size:
3972 1115 1071 85 31
BsmI GAATG_Cn'
Cuts at: 2247 3785 3878 2247
Size: 1538 93 4643
Fragments arranged by size:
4643 1538 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2438 2438
Size: 6274
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2344 3189 3220 3292 3593 4335
Size: 399 471 83 845 31 72 301 742
Cuts at: 4335 4420 5491 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 845 742 471 399 301 85
83 72 31
BspEI T'CCGG_A
Cuts at: 5937 5937
Size: 6274
BspHI T'CATG_A
Cuts at: 4163 5893 4163
Size: 1730 4544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2698 3168 3629 844
Size: 1854 470 461 3489
Fragments arranged by size:
3489 1854 470 461
BsrBI CCG'CTC
Cuts at: 1394 1823 2338 4161 1394
Size: 429 515 1823 3507
Fragments arranged by size:
3507 1823 515 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3401 4769 4943 65
Size: 1183 2153 1368 174 1396
Fragments arranged by size:
2153 1396 1368 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3063 3093 4918 5105 1700
Size: 110 1253 30 1825 187 2869
Fragments arranged by size:
2869 1825 1253 187 110 30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6274
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6274
BssSI C'ACGA_G
Cuts at: 4334 5346 4334
Size: 1012 5262
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6274
BstBI TT'CG_AA
Cuts at: 5078 5078
Size: 6274
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6274
BstYI r'GATC_y
Cuts at: 1972 2042 2525 2755 3496 3604 3971 4356 4373
Size: 70 483 230 741 108 367 385 17
Cuts at: 4373 5825 5911 5923 6269 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 741 483 385 367 346 230
108 86 70 17 12
Bsu36I CC'TnA_GG
Cuts at: 2385 2419 2448 2385
Size: 34 29 6211
Fragments arranged by size:
6211 34 29
BtgI C'CryG_G
Cuts at: 513 1065 2004 2019 2622 3098 513
Size: 552 939 15 603 476 3689
Fragments arranged by size:
3689 939 603 552 476 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5535
Fragments arranged by size:
5535 683 56
BtsI GCAGTG_nn'
Cuts at: 2088 3571 3790 4584 4604 2088
Size: 1483 219 794 20 3758
Fragments arranged by size:
3758 1483 794 219 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6239
DraI TTT'AAA
Cuts at: 3578 3578
Size: 6274
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5281 809
Size: 507 3965 1802
Fragments arranged by size:
3965 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 3095 3231
Size: 54 1425 50 275 18 186 1079 136
Cuts at: 3231 3721 4610 6239 8
Size: 490 889 1629 43
Fragments arranged by size:
1629 1425 1079 889 490 275 186 136
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3721 1537
Size: 275 1909 4090
Fragments arranged by size:
4090 1909 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2546 2877 4204 1353
Size: 1193 331 1327 3423
Fragments arranged by size:
3423 1327 1193 331
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2072 4861 5235 5381 387
Size: 1064 138 483 2789 374 146 1280
Fragments arranged by size:
2789 1280 1064 483 374 146 138
EcoICRI GAG'CTC
Cuts at: 3218 3218
Size: 6274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2427 2734 3026 4337 4934 5721 5989
Size: 393 795 307 292 1311 597 787 268
Cuts at: 5989 1239
Size: 1524
Fragments arranged by size:
1524 1311 795 787 597 393 307 292
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2348 2846 2348
Size: 498 5776
EcoO109I rG'GnC_Cy
Cuts at: 2341 2653 2341
Size: 312 5962
EcoRI G'AATT_C
Cuts at: 3036 3583 3961 3036
Size: 547 378 5349
Fragments arranged by size:
5349 547 378
FspI TGC'GCA
Cuts at: 4780 4780
Size: 6274
HaeII r_GCGC'y
Cuts at: 1678 5421 1678
Size: 3743 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2510 2517 2542
Size: 32 135 32 455 32 451 7 25
Cuts at: 2542 2549 2879 2911 2985 3017 4915 4947 4989
Size: 7 330 32 74 32 1898 32 42
Cuts at: 4989 5021 1373
Size: 32 2626
Fragments arranged by size:
2626 1898 455 451 330 135 74 42
32 32 32 32 32 32 32 25
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2498 3618 3864 669
Size: 988 281 560 1120 246 3079
Fragments arranged by size:
3079 1120 988 560 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6274
HpaI GTT'AAC
Cuts at: 3864 3864
Size: 6274
KpnI G_GTAC'C
Cuts at: 3599 3599
Size: 6274
MfeI C'AATT_G
Cuts at: 3873 3873
Size: 6274
MluI A'CGCG_T
Cuts at: 6150 6150
Size: 6274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2267 5388 5572 1305
Size: 962 3121 184 2007
Fragments arranged by size:
3121 2007 962 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3233 10
Size: 54 1425 343 1401 3051
Fragments arranged by size:
3051 1425 1401 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2861 3141 4232 4591 4750 518
Size: 648 1695 280 1091 359 159 2042
Fragments arranged by size:
2042 1695 1091 648 359 280 159
MspA1I CmG'CkG
Cuts at: 1606 2282 2405 3690 4367 5515 5760 1606
Size: 676 123 1285 677 1148 245 2120
Fragments arranged by size:
2120 1285 1148 677 676 245 123
NaeI GCC'GGC
Cuts at: 3065 3095 3065
Size: 30 6244
NcoI C'CATG_G
Cuts at: 513 1065 2004 2622 3098 513
Size: 552 939 618 476 3689
Fragments arranged by size:
3689 939 618 552 476
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6274
NgoMIV G'CCGG_C
Cuts at: 3063 3093 3063
Size: 30 6244
NheI G'CTAG_C
Cuts at: 1052 2944 1052
Size: 1892 4382
NotI GC'GGCC_GC
Cuts at: 3721 3721
Size: 6274
NruI TCG'CGA
Cuts at: 2031 2031
Size: 6274
NspI r_CATG'y
Cuts at: 1132 1229 2824 3416 3632 5177 1132
Size: 97 1595 592 216 1545 2229
Fragments arranged by size:
2229 1595 1545 592 216 97
PasI CC'CwG_GG
Cuts at: 1348 2069 2650 1348
Size: 721 581 4972
Fragments arranged by size:
4972 721 581
PciI A'CATG_T
Cuts at: 3412 5173 3412
Size: 1761 4513
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2183 2656 1108
Size: 1075 473 4726
Fragments arranged by size:
4726 1075 473
PfoI T'CCnGG_A
Cuts at: 2045 2045
Size: 6274
PmeI GTTT'AAAC
Cuts at: 3578 3578
Size: 6274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 5856 5888 4343
Size: 32 1481 32 4729
Fragments arranged by size:
4729 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6274
PsiI TTA'TAA
Cuts at: 3844 3844
Size: 6274
PspOMI G'GGCC_C
Cuts at: 2340 2340
Size: 6274
PspXI vC'TCGA_Gb
Cuts at: 3285 3285
Size: 6274
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3531 3563 3531
Size: 32 6242
PstI C_TGCA'G
Cuts at: 830 2118 2203 2643 3175 3626 830
Size: 1288 85 440 532 451 3478
Fragments arranged by size:
3478 1288 532 451 440 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6274
PvuII CAG'CTG
Cuts at: 2282 2405 2282
Size: 123 6151
SacI G_AGCT'C
Cuts at: 3220 3220
Size: 6274
SalI G'TCGA_C
Cuts at: 1936 3616 1936
Size: 1680 4594
SbfI CC_TGCA'GG
Cuts at: 3626 3626
Size: 6274
ScaI AGT'ACT
Cuts at: 2645 4522 6234 2645
Size: 1877 1712 2685
Fragments arranged by size:
2685 1877 1712
SexAI A'CCwGG_T
Cuts at: 3570 3570
Size: 6274
SfcI C'TryA_G
Cuts at: 826 1045 2114 2199 2639 3171 3622 4757 5438
Size: 219 1069 85 440 532 451 1135 681
Cuts at: 5438 5629 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1069 681 532 451 440 219
191 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 2339 2339
Size: 6274
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6274
SmaI CCC'GGG
Cuts at: 1872 3601 1872
Size: 1729 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3285 4378 5279 5541 820
Size: 197 926 1342 1093 901 262 1553
Fragments arranged by size:
1553 1342 1093 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4065 493
Size: 3572 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6274
SphI G_CATG'C
Cuts at: 1132 2824 3632 1132
Size: 1692 808 3774
Fragments arranged by size:
3774 1692 808
SspI AAT'ATT
Cuts at: 5 52 4198 5
Size: 47 4146 2081
Fragments arranged by size:
4146 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6274
StyI C'CwwG_G
Cuts at: 513 1065 2004 2622 3098 3201 3713 513
Size: 552 939 618 476 103 512 3074
Fragments arranged by size:
3074 939 618 552 512 476 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2631 4301 4318 4471 4656 5101 325
Size: 1255 1051 1670 17 153 185 445 1498
Fragments arranged by size:
1670 1498 1255 1051 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2559 2643 4520
Size: 274 80 33 51 1443 582 84 1877
Cuts at: 4520 6232 96
Size: 1712 138
Fragments arranged by size:
1877 1712 1443 582 274 138 84 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6234 6266 6234
Size: 32 6242
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6274
XbaI T'CTAG_A
Cuts at: 3610 3610
Size: 6274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3208 2011
Size: 1197 5077
XhoI C'TCGA_G
Cuts at: 1943 3285 1943
Size: 1342 4932
XmaI C'CCGG_G
Cuts at: 1870 3599 1870
Size: 1729 4545
XmnI GAAnn'nnTTC
Cuts at: 4403 4403
Size: 6274
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4082 276
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NarI NsiI PacI PmlI PpuMI RsrII SacII SanDI
SapI SfoI SrfI SwaI