Restriction map for
pFN21AE5648
(Circular) (Six-base) MAPSORT of: pFN21AE5648.seq Check: 6603 from: 1 to: 6160
pFN21AE5648 6160 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2037 2037
Size: 6160
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3503 1937
Size: 1566 4594
Acc65I G'GTAC_C
Cuts at: 3481 3481
Size: 6160
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2422 2730 2806 4223 5607 5875 1632
Size: 790 308 76 1417 1384 268 1917
Fragments arranged by size:
1917 1417 1384 790 308 268 76
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 2943 5059 6036 1203
Size: 1740 2116 977 1327
Fragments arranged by size:
2116 1740 1327 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6160
AlwNI CAG_nnn'CTG
Cuts at: 2031 2835 3903 5475 2031
Size: 804 1068 1572 2716
Fragments arranged by size:
2716 1572 1068 804
ApaLI G'TGCA_C
Cuts at: 4217 5373 4217
Size: 1156 5004
ApoI r'AATT_y
Cuts at: 1493 3469 3695 3847 5956 1493
Size: 1976 226 152 2109 1697
Fragments arranged by size:
2109 1976 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3359 4714 160
Size: 872 2327 1355 1606
Fragments arranged by size:
2327 1606 1355 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 2153 2183 2423 3485 1870
Size: 73 210 30 240 1062 4545
Fragments arranged by size:
4545 1062 240 210 73 30
AvrII C'CTAG_G
Cuts at: 2920 2920
Size: 6160
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2930 2963 2930
Size: 33 6127
BamHI G'GATC_C
Cuts at: 3490 3857 3490
Size: 367 5793
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2049 2286 2567 3481 4936
Size: 325 217 627 262 237 281 914 1455
Cuts at: 4936 618
Size: 1842
Fragments arranged by size:
1842 1455 914 627 325 281 262 237
217
BanII G_rGCy'C
Cuts at: 1391 2154 3479 1391
Size: 763 1325 4072
Fragments arranged by size:
4072 1325 763
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2711 928
Size: 971 812 4377
Fragments arranged by size:
4377 971 812
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4351 4385 1903
Size: 34 50 34 2330 34 3678
Fragments arranged by size:
3678 2330 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2958 4052 5262 2958
Size: 1094 1210 3856
Fragments arranged by size:
3856 1210 1094
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6160
BfrBI ATG'CAT
Cuts at: 3403 3403
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 2295 4771
Size: 107 122 71 631 605 355 268 2476
Cuts at: 4771 136
Size: 1525
Fragments arranged by size:
2476 1525 631 605 355 268 122 107
71
BglII A'GATC_T
Cuts at: 2217 6155 2217
Size: 3938 2222
BlpI GC'TnA_GC
Cuts at: 2261 3577 2261
Size: 1316 4844
Bme1580I G_kGCm'C
Cuts at: 1790 4221 5377 1790
Size: 2431 1156 2573
Fragments arranged by size:
2573 2431 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6160
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3891 4849 442
Size: 416 53 83 399 24 2474 958 1753
Fragments arranged by size:
2474 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2211 2768 4820 1239
Size: 972 557 2052 2579
Fragments arranged by size:
2579 2052 972 557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 4285 5150 5448 2537
Size: 1748 865 298 3249
Fragments arranged by size:
3249 1748 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4823 882
Size: 617 3324 2219
Fragments arranged by size:
3324 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3951 493
Size: 711 2747 2702
Fragments arranged by size:
2747 2702 711
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2391 3967 4349 4984
Size: 53 83 186 936 858 1576 382 635
Cuts at: 4984 275
Size: 1451
Fragments arranged by size:
1576 1451 936 858 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4593 4991 5265 5412 5823 4593
Size: 398 274 147 411 4930
Fragments arranged by size:
4930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2118 2148 1178
Size: 30 165 30 715 30 5190
Fragments arranged by size:
5190 715 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2724 1186
Size: 1538 4622
BseYI C'CCAG_C
Cuts at: 5363 5859 5363
Size: 496 5664
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5587
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3610 4371 5399 1540
Size: 275 186 1609 761 1028 2301
Fragments arranged by size:
2301 1609 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 2172 4221 4306 5377 2154
Size: 18 2049 85 1071 2937
Fragments arranged by size:
2937 2049 1071 85 18
BsmI GAATG_Cn'
Cuts at: 3671 3764 3671
Size: 93 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2172 3479 4221 4306 5377 1391
Size: 399 364 18 1307 742 85 1071 2174
Fragments arranged by size:
2174 1307 1071 742 399 364 85 18
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2037 2365 3515 844
Size: 1193 328 1150 3489
Fragments arranged by size:
3489 1193 1150 328
BsrBI CCG'CTC
Cuts at: 1394 1823 4047 1394
Size: 429 2224 3507
Fragments arranged by size:
3507 2224 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2962 4655 4829 65
Size: 1183 1714 1693 174 1396
Fragments arranged by size:
1714 1693 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4804 4991 1700
Size: 110 2994 187 2869
Fragments arranged by size:
2994 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6160
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6160
BssSI C'ACGA_G
Cuts at: 2013 4220 5232 2013
Size: 2207 1012 2941
Fragments arranged by size:
2941 2207 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6160
BstBI TT'CG_AA
Cuts at: 3293 4964 3293
Size: 1671 4489
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6160
BstYI r'GATC_y
Cuts at: 1972 2217 2596 3490 3857 4242 4259 5711 5797
Size: 245 379 894 367 385 17 1452 86
Cuts at: 5797 5809 6155 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 894 385 379 367 346 245
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5608
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5421
Fragments arranged by size:
5421 683 56
BtsI GCAGTG_nn'
Cuts at: 2836 3676 4470 4490 2836
Size: 840 794 20 4506
Fragments arranged by size:
4506 840 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6125
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3198 5167 809
Size: 507 1882 1969 1802
Fragments arranged by size:
1969 1882 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 2974 3607
Size: 54 1425 50 275 18 504 640 633
Cuts at: 3607 4496 6125 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 640 633 504 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3607 1537
Size: 275 1795 4090
Fragments arranged by size:
4090 1795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2280 3163 3323 4090 1353
Size: 927 883 160 767 3423
Fragments arranged by size:
3423 927 883 767 160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4747 5121 5267 387
Size: 1064 138 3158 374 146 1280
Fragments arranged by size:
3158 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2152 2152
Size: 6160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2422 2730 2768 2806 4223 4820
Size: 393 579 211 308 38 38 1417 597
Cuts at: 4820 5607 5875 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1417 787 597 579 393 308 268
211 38 38
EcoO109I rG'GnC_Cy
Cuts at: 2028 2411 3025 3173 2028
Size: 383 614 148 5015
Fragments arranged by size:
5015 614 383 148
EcoRI G'AATT_C
Cuts at: 3469 3847 3469
Size: 378 5782
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 2210 5307 1678
Size: 532 3097 2531
Fragments arranged by size:
3097 2531 532
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4801 4833 4875 4907 1373
Size: 32 135 32 3229 32 42 32 2626
Fragments arranged by size:
3229 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2103 3504 3750 669
Size: 988 281 165 1401 246 3079
Fragments arranged by size:
3079 1401 988 281 246 165
HindIII A'AGCT_T
Cuts at: 748 2712 748
Size: 1964 4196
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KpnI G_GTAC'C
Cuts at: 3485 3485
Size: 6160
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5274 5458 1305
Size: 3969 184 2007
Fragments arranged by size:
3969 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4338
Fragments arranged by size:
4338 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3386 4118 4477 4636 518
Size: 648 2220 732 359 159 2042
Fragments arranged by size:
2220 2042 732 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3070 3576 4253 5401 5646 1606
Size: 1464 506 677 1148 245 2120
Fragments arranged by size:
2120 1464 1148 677 506 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5608
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NsiI A_TGCA'T
Cuts at: 3405 3405
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 2135 2947 3043 3518 5063 1132
Size: 97 906 812 96 475 1545 2229
Fragments arranged by size:
2229 1545 906 812 475 97 96
PasI CC'CwG_GG
Cuts at: 1348 2886 1348
Size: 1538 4622
PciI A'CATG_T
Cuts at: 2943 5059 2943
Size: 2116 4044
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6160
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 5742 5774 4229
Size: 32 1481 32 4615
Fragments arranged by size:
4615 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3025 3025
Size: 6160
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6160
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PspXI vC'TCGA_Gb
Cuts at: 2153 2153
Size: 6160
PstI C_TGCA'G
Cuts at: 830 2048 2455 3512 830
Size: 1218 407 1057 3478
Fragments arranged by size:
3478 1218 1057 407
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
PvuII CAG'CTG
Cuts at: 3070 3070
Size: 6160
SacI G_AGCT'C
Cuts at: 2154 2154
Size: 6160
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 4408 6120 4408
Size: 1712 4448
SfcI C'TryA_G
Cuts at: 826 1045 2044 2451 3508 4643 5324 5515 826
Size: 219 999 407 1057 1135 681 191 1471
Fragments arranged by size:
1471 1135 1057 999 681 407 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6160
SmaI CCC'GGG
Cuts at: 1872 2185 2425 3487 1872
Size: 313 240 1062 4545
Fragments arranged by size:
4545 1062 313 240
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 2516 4264 5165 5427 820
Size: 197 926 210 363 1748 901 262 1553
Fragments arranged by size:
1748 1553 926 901 363 262 210 197
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 3043 3518 1132
Size: 1911 475 3774
Fragments arranged by size:
3774 1911 475
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6160
StyI C'CwwG_G
Cuts at: 513 1065 2307 2571 2920 3599 513
Size: 552 1242 264 349 679 3074
Fragments arranged by size:
3074 1242 679 552 349 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2187 2274 3048 4187 4204 4357 4542
Size: 1255 607 87 774 1139 17 153 185
Cuts at: 4542 4987 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1139 774 607 445 185 153
87 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4406 6118 96
Size: 274 80 33 51 1443 2429 1712 138
Fragments arranged by size:
2429 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2118 2150 6120 6152 2118
Size: 32 3970 32 2126
Fragments arranged by size:
3970 2126 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6160
XbaI T'CTAG_A
Cuts at: 3496 3496
Size: 6160
XhoI C'TCGA_G
Cuts at: 1943 2153 1943
Size: 210 5950
XmaI C'CCGG_G
Cuts at: 1870 2183 2423 3485 1870
Size: 313 240 1062 4545
Fragments arranged by size:
4545 1062 313 240
XmnI GAAnn'nnTTC
Cuts at: 4289 4289
Size: 6160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI