Restriction map for
pFN21AE5644
(Circular) (Six-base) MAPSORT of: pFN21AE5644.seq Check: 7467 from: 1 to: 6136
pFN21AE5644 6136 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 1937
Size: 1542 4594
Acc65I G'GTAC_C
Cuts at: 2128 3457 2128
Size: 1329 4807
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4199 5583 5851 1632
Size: 2567 1384 268 1917
Fragments arranged by size:
2567 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2248 820
Size: 197 1231 4708
Fragments arranged by size:
4708 1231 197
AflIII A'CryG_T
Cuts at: 1203 5035 6012 1203
Size: 3832 977 1327
Fragments arranged by size:
3832 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 4865 4865
Size: 6136
AleI CACnn'nnGTG
Cuts at: 1166 2513 1166
Size: 1347 4789
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6136
AlwNI CAG_nnn'CTG
Cuts at: 2213 2791 2845 3879 5451 2213
Size: 578 54 1034 1572 2898
Fragments arranged by size:
2898 1572 1034 578 54
ApaLI G'TGCA_C
Cuts at: 4193 5349 4193
Size: 1156 4980
ApoI r'AATT_y
Cuts at: 1493 3128 3445 3671 3823 5932 1493
Size: 1635 317 226 152 2109 1697
Fragments arranged by size:
2109 1697 1635 317 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 3461 1870
Size: 73 1518 4545
Fragments arranged by size:
4545 1518 73
BamHI G'GATC_C
Cuts at: 2597 2757 2993 3466 3833 2597
Size: 160 236 473 367 4900
Fragments arranged by size:
4900 473 367 236 160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 3457 4912 618
Size: 325 217 627 341 1329 1455 1842
Fragments arranged by size:
1842 1455 1329 627 341 325 217
BanII G_rGCy'C
Cuts at: 1391 3258 3455 1391
Size: 1867 197 4072
Fragments arranged by size:
4072 1867 197
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 928
Size: 971 429 4736
Fragments arranged by size:
4736 971 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2507 2541 4327 4361 1903
Size: 34 570 34 1786 34 3678
Fragments arranged by size:
3678 1786 570 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 2500 1548
Size: 952 5184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4747 136
Size: 107 122 71 631 605 3075 1525
Fragments arranged by size:
3075 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6131 6131
Size: 6136
BlpI GC'TnA_GC
Cuts at: 3553 3553
Size: 6136
Bme1580I G_kGCm'C
Cuts at: 1790 2044 4197 5353 1790
Size: 254 2153 1156 2573
Fragments arranged by size:
2573 2153 1156 254
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6136
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2756 2855 3867
Size: 416 53 83 399 24 1339 99 1012
Cuts at: 3867 4825 442
Size: 958 1753
Fragments arranged by size:
1753 1339 1012 958 416 399 99 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2819 2941 3301 3353 4796 1239
Size: 1580 122 360 52 1443 2579
Fragments arranged by size:
2579 1580 1443 360 122 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2124 2256 2280 2582 2853 3323 4261 5126 5424
Size: 132 24 302 271 470 938 865 298
Cuts at: 5424 2124
Size: 2836
Fragments arranged by size:
2836 938 865 470 302 298 271 132
24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 4799 882
Size: 617 1041 2259 2219
Fragments arranged by size:
2259 2219 1041 617
BsaAI yAC'GTr
Cuts at: 493 1204 3927 493
Size: 711 2723 2702
Fragments arranged by size:
2723 2702 711
BsaBI GATnn'nnATC
Cuts at: 6130 6130
Size: 6136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3943 4325 4960 275
Size: 53 83 186 936 2410 382 635 1451
Fragments arranged by size:
2410 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4569 4967 5241 5388 5799 4569
Size: 398 274 147 411 4906
Fragments arranged by size:
4906 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2112 2142 2727 2757 3299
Size: 30 165 30 709 30 585 30 542
Cuts at: 3299 3329 1178
Size: 30 3985
Fragments arranged by size:
3985 709 585 542 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2558 1186
Size: 952 420 4764
Fragments arranged by size:
4764 952 420
BseYI C'CCAG_C
Cuts at: 2043 3364 5339 5835 2043
Size: 1321 1975 496 2344
Fragments arranged by size:
2344 1975 1321 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2199 2537 1163
Size: 573 463 338 4762
Fragments arranged by size:
4762 573 463 338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3586 4347 5375 1540
Size: 275 186 1585 761 1028 2301
Fragments arranged by size:
2301 1585 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 4197 4282 5353 2313
Size: 1884 85 1071 3096
Fragments arranged by size:
3096 1884 1071 85
BsmI GAATG_Cn'
Cuts at: 2889 3647 3740 2889
Size: 758 93 5285
Fragments arranged by size:
5285 758 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2313 3258 3455 4197 4282 5353
Size: 399 254 269 945 197 742 85 1071
Cuts at: 5353 1391
Size: 2174
Fragments arranged by size:
2174 1071 945 742 399 269 254 197
85
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 2811 4025 5755 2811
Size: 1214 1730 3192
Fragments arranged by size:
3192 1730 1214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3491 844
Size: 2647 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3375 4023 1394
Size: 429 1552 648 3507
Fragments arranged by size:
3507 1552 648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4631 4805 65
Size: 1183 3383 174 1396
Fragments arranged by size:
3383 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4780 4967 1700
Size: 110 2970 187 2869
Fragments arranged by size:
2970 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6136
BssSI C'ACGA_G
Cuts at: 2748 4196 5208 2748
Size: 1448 1012 3676
Fragments arranged by size:
3676 1448 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6136
BstBI TT'CG_AA
Cuts at: 4940 4940
Size: 6136
BstEII G'GTnAC_C
Cuts at: 1184 2624 1184
Size: 1440 4696
BstXI CCAn_nnnn'nTGG
Cuts at: 2490 3287 3325 2490
Size: 797 38 5301
Fragments arranged by size:
5301 797 38
BstYI r'GATC_y
Cuts at: 1972 2009 2209 2597 2757 2936 2993 3466 3833
Size: 37 200 388 160 179 57 473 367
Cuts at: 3833 4218 4235 5687 5773 5785 6131 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 473 388 385 367 346 200
179 160 86 57 37 17 12
Bsu36I CC'TnA_GG
Cuts at: 2541 2541
Size: 6136
BtgI C'CryG_G
Cuts at: 513 1065 2004 3417 513
Size: 552 939 1413 3232
Fragments arranged by size:
3232 1413 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5397
Fragments arranged by size:
5397 683 56
BtsI GCAGTG_nn'
Cuts at: 3652 4446 4466 3652
Size: 794 20 5322
Fragments arranged by size:
5322 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3133 3440 3133
Size: 307 5829
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5143 809
Size: 507 3827 1802
Fragments arranged by size:
3827 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2636 3583 4472
Size: 54 1425 50 275 18 806 947 889
Cuts at: 4472 6101 8
Size: 1629 43
Fragments arranged by size:
1629 1425 947 889 806 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3583 1537
Size: 275 1771 4090
Fragments arranged by size:
4090 1771 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3018 3246 4066 1353
Size: 1665 228 820 3423
Fragments arranged by size:
3423 1665 820 228
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4723 5097 5243 387
Size: 1064 138 3134 374 146 1280
Fragments arranged by size:
3134 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2819 2941 3301 3353 4199 4796 5583
Size: 393 1187 122 360 52 846 597 787
Cuts at: 5583 5851 1239
Size: 268 1524
Fragments arranged by size:
1524 1187 846 787 597 393 360 268
122 52
EcoNI CCTnn'n_nnAGG
Cuts at: 2664 3278 2664
Size: 614 5522
EcoO109I rG'GnC_Cy
Cuts at: 3116 3228 3116
Size: 112 6024
EcoRI G'AATT_C
Cuts at: 3445 3823 3445
Size: 378 5758
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 3425 5283 1678
Size: 1747 1858 2531
Fragments arranged by size:
2531 1858 1747
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 2589 2621 2851
Size: 32 135 32 905 32 80 32 230
Cuts at: 2851 2883 3299 3331 4777 4809 4851 4883 1373
Size: 32 416 32 1446 32 42 32 2626
Fragments arranged by size:
2626 1446 905 416 230 135 80 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2767 2905 3480 3726 669
Size: 988 281 829 138 575 246 3079
Fragments arranged by size:
3079 988 829 575 281 246 138
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6136
HpaI GTT'AAC
Cuts at: 2905 3726 2905
Size: 821 5315
KpnI G_GTAC'C
Cuts at: 2132 3461 2132
Size: 1329 4807
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3009 5250 5434 1305
Size: 1704 2241 184 2007
Fragments arranged by size:
2241 2007 1704 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2638 10
Size: 54 1425 343 806 3508
Fragments arranged by size:
3508 1425 806 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 4094 4453 4612 518
Size: 648 1347 1581 359 159 2042
Fragments arranged by size:
2042 1581 1347 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3552 4229 5377 5622 1606
Size: 1946 677 1148 245 2120
Fragments arranged by size:
2120 1946 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4645
Fragments arranged by size:
4645 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 2773 3494 5039 1132
Size: 97 1544 721 1545 2229
Fragments arranged by size:
2229 1545 1544 721 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6136
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2435 2607 2845 1108
Size: 1327 172 238 4399
Fragments arranged by size:
4399 1327 238 172
PfoI T'CCnGG_A
Cuts at: 2784 2784
Size: 6136
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6136
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PstI C_TGCA'G
Cuts at: 830 2837 3387 3488 830
Size: 2007 550 101 3478
Fragments arranged by size:
3478 2007 550 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SapI GCTCTTCn'nnn_
Cuts at: 3246 3246
Size: 6136
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 4384 6096 4384
Size: 1712 4424
SfcI C'TryA_G
Cuts at: 826 1045 2833 3383 3484 4619 5300 5491 826
Size: 219 1788 550 101 1135 681 191 1471
Fragments arranged by size:
1788 1471 1135 681 550 219 191 101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 3463 1872
Size: 1591 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2139 2248 2271 2295 2561 2868
Size: 197 926 196 109 23 24 266 307
Cuts at: 2868 3302 4240 5141 5403 820
Size: 434 938 901 262 1553
Fragments arranged by size:
1553 938 926 901 434 307 266 262
197 196 109 24 23
SnaBI TAC'GTA
Cuts at: 493 3927 493
Size: 3434 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 2675 4060 5
Size: 47 2623 1385 2081
Fragments arranged by size:
2623 2081 1385 47
StuI AGG'CCT
Cuts at: 1517 3152 1517
Size: 1635 4501
StyI C'CwwG_G
Cuts at: 513 1065 2004 3153 3575 513
Size: 552 939 1149 422 3074
Fragments arranged by size:
3074 1149 939 552 422
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2340 4163 4180 4333 4518 4963 325
Size: 1255 760 1823 17 153 185 445 1498
Fragments arranged by size:
1823 1498 1255 760 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4382 6094 96
Size: 274 80 33 51 1443 2405 1712 138
Fragments arranged by size:
2405 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6096 6128 6096
Size: 32 6104
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6136
XbaI T'CTAG_A
Cuts at: 3472 3472
Size: 6136
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6136
XmaI C'CCGG_G
Cuts at: 1870 3461 1870
Size: 1591 4545
XmnI GAAnn'nnTTC
Cuts at: 2676 4265 2676
Size: 1589 4547
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI