Restriction map for
pFN21AE5643
(Circular) (Six-base) MAPSORT of: pFN21AE5643.seq Check: 3823 from: 1 to: 6121
pFN21AE5643 6121 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3464 1937
Size: 1527 4594
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2851 4184 5568 5836 1632
Size: 1219 1333 1384 268 1917
Fragments arranged by size:
1917 1384 1333 1219 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 2768 5020 5997 1203
Size: 1565 2252 977 1327
Fragments arranged by size:
2252 1565 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6121
AlwNI CAG_nnn'CTG
Cuts at: 2077 3864 5436 2077
Size: 1787 1572 2762
Fragments arranged by size:
2762 1787 1572
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 2486 2817 3069 3408 3430 3656 3808 5917
Size: 993 331 252 339 22 226 152 2109
Cuts at: 5917 1493
Size: 1697
Fragments arranged by size:
2109 1697 993 339 331 252 226 152
22
AseI AT'TA_AT
Cuts at: 160 1032 2805 4675 160
Size: 872 1773 1870 1606
Fragments arranged by size:
1870 1773 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3446 1870
Size: 73 1503 4545
Fragments arranged by size:
4545 1503 73
BamHI G'GATC_C
Cuts at: 3451 3818 3451
Size: 367 5754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3396 3442 4897 618
Size: 325 217 627 1609 46 1455 1842
Fragments arranged by size:
1842 1609 1455 627 325 217 46
BanII G_rGCy'C
Cuts at: 1391 3440 1391
Size: 2049 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 1903
Size: 34 2375 34 3678
Fragments arranged by size:
3678 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2447 4013 5223 2447
Size: 1566 1210 3345
Fragments arranged by size:
3345 1566 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BfrBI ATG'CAT
Cuts at: 2737 2737
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 136
Size: 107 122 71 631 605 3060 1525
Fragments arranged by size:
3060 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2965 6116 2965
Size: 3151 2970
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 4182 5338 1790
Size: 2392 1156 2573
Fragments arranged by size:
2573 2392 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2399 3087 3171
Size: 416 53 83 399 24 982 688 84
Cuts at: 3171 3852 4810 442
Size: 681 958 1753
Fragments arranged by size:
1753 982 958 688 681 416 399 84
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 3400 4781 1239
Size: 899 1262 1381 2579
Fragments arranged by size:
2579 1381 1262 899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2200 4246 5111 5409 2200
Size: 2046 865 298 2912
Fragments arranged by size:
2912 2046 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4784 882
Size: 617 3285 2219
Fragments arranged by size:
3285 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3912 493
Size: 711 2708 2702
Fragments arranged by size:
2708 2702 711
BsaBI GATnn'nnATC
Cuts at: 2640 6115 2640
Size: 3475 2646
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3928 4310 4945 275
Size: 53 83 186 936 2395 382 635 1451
Fragments arranged by size:
2395 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3291 4554 4952 5226 5373 5784 3291
Size: 1263 398 274 147 411 3628
Fragments arranged by size:
3628 1263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 1178
Size: 30 165 30 789 30 5077
Fragments arranged by size:
5077 789 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2578 1186
Size: 1392 4729
BseYI C'CCAG_C
Cuts at: 2227 5324 5820 2227
Size: 3097 496 2528
Fragments arranged by size:
3097 2528 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2262 1163
Size: 573 526 5022
Fragments arranged by size:
5022 573 526
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4182 4267 5338 4182
Size: 85 1071 4965
Fragments arranged by size:
4965 1071 85
BsmI GAATG_Cn'
Cuts at: 2612 3362 3632 3725 2612
Size: 750 270 93 5008
Fragments arranged by size:
5008 750 270 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3440 4182 4267 5338 1391
Size: 399 1650 742 85 1071 2174
Fragments arranged by size:
2174 1650 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 3291 5784 3291
Size: 2493 3628
BspHI T'CATG_A
Cuts at: 2809 3061 4010 5740 2809
Size: 252 949 1730 3190
Fragments arranged by size:
3190 1730 949 252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3476 844
Size: 2632 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4008 1394
Size: 429 2185 3507
Fragments arranged by size:
3507 2185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4616 4790 65
Size: 1183 3368 174 1396
Fragments arranged by size:
3368 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4765 4952 1700
Size: 110 2955 187 2869
Fragments arranged by size:
2955 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2100 2425 96
Size: 2004 325 3792
Fragments arranged by size:
3792 2004 325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6121
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6121
BstYI r'GATC_y
Cuts at: 1972 2369 2965 3451 3818 4203 4220 5672 5758
Size: 397 596 486 367 385 17 1452 86
Cuts at: 5758 5770 6116 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 596 486 397 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2561 2561
Size: 6121
BtgI C'CryG_G
Cuts at: 513 1065 2204 513
Size: 552 1139 4430
Fragments arranged by size:
4430 1139 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 3637 4431 4451 3637
Size: 794 20 5307
Fragments arranged by size:
5307 794 20
ClaI AT'CG_AT
Cuts at: 1554 3057 1554
Size: 1503 4618
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2236 3568 4457
Size: 54 1425 50 275 18 406 1332 889
Cuts at: 4457 6086 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1332 889 406 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2017 2211 2602 3077 4051 1353
Size: 664 194 391 475 974 3423
Fragments arranged by size:
3423 974 664 475 391 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2851 3400 4184 4781 5568 5836
Size: 393 506 713 549 784 597 787 268
Cuts at: 5836 1239
Size: 1524
Fragments arranged by size:
1524 787 784 713 597 549 506 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2667 3255 2667
Size: 588 5533
EcoRI G'AATT_C
Cuts at: 3408 3430 3808 3408
Size: 22 378 5721
Fragments arranged by size:
5721 378 22
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 5268 1678
Size: 3590 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2390 2422 4762 4794 4836
Size: 32 135 32 818 32 2340 32 42
Cuts at: 4836 4868 1373
Size: 32 2626
Fragments arranged by size:
2626 2340 818 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3465 3711 669
Size: 988 281 1527 246 3079
Fragments arranged by size:
3079 1527 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2630 748
Size: 1882 4239
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2166 2190 5235 5419 1305
Size: 861 24 3045 184 2007
Fragments arranged by size:
3045 2007 861 184 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4299
Fragments arranged by size:
4299 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2601 4079 4438 4597 518
Size: 648 1435 1478 359 159 2042
Fragments arranged by size:
2042 1478 1435 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 3537 4214 5362 5607 1606
Size: 468 1463 677 1148 245 2120
Fragments arranged by size:
2120 1463 1148 677 468 245
NcoI C'CATG_G
Cuts at: 513 1065 2204 513
Size: 552 1139 4430
Fragments arranged by size:
4430 1139 552
NdeI CA'TA_TG
Cuts at: 387 3020 387
Size: 2633 3488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NsiI A_TGCA'T
Cuts at: 2739 2739
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 2573 2772 3479 5024 1132
Size: 97 1344 199 707 1545 2229
Fragments arranged by size:
2229 1545 1344 707 199 97
PasI CC'CwG_GG
Cuts at: 1348 2017 2059 1348
Size: 669 42 5410
Fragments arranged by size:
5410 669 42
PciI A'CATG_T
Cuts at: 2768 5020 2768
Size: 2252 3869
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6121
PfoI T'CCnGG_A
Cuts at: 2786 2786
Size: 6121
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3255 3255
Size: 6121
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 3691 3691
Size: 6121
PstI C_TGCA'G
Cuts at: 830 3473 830
Size: 2643 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SapI GCTCTTCn'nnn_
Cuts at: 2602 2602
Size: 6121
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2518 3259 3469 4604 5285 5476 826
Size: 219 1473 741 210 1135 681 191 1471
Fragments arranged by size:
1473 1471 1135 741 681 219 210 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2179 4225 5126 5388 820
Size: 197 926 236 2046 901 262 1553
Fragments arranged by size:
2046 1553 926 901 262 236 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 2573 3479 1132
Size: 1441 906 3774
Fragments arranged by size:
3774 1441 906
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 3032 1517
Size: 1515 4606
StyI C'CwwG_G
Cuts at: 513 1065 2204 3560 513
Size: 552 1139 1356 3074
Fragments arranged by size:
3074 1356 1139 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 4148 4165 4318 4503 4948 325
Size: 1255 492 2076 17 153 185 445 1498
Fragments arranged by size:
2076 1498 1255 492 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2100 2425 4367
Size: 274 80 33 51 1443 123 325 1942
Cuts at: 4367 6079 96
Size: 1712 138
Fragments arranged by size:
1942 1712 1443 325 274 138 123 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6081 6113 6081
Size: 32 6089
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 2864 4250 2864
Size: 1386 4735
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI