Restriction map for
pFN21AE5641
(Circular) (Six-base) MAPSORT of: pFN21AE5641.seq Check: 247 from: 1 to: 6454
pFN21AE5641 6454 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4264 278
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3797 1937
Size: 1860 4594
Acc65I G'GTAC_C
Cuts at: 3542 3775 3542
Size: 233 6221
AclI AA'CG_TT
Cuts at: 1563 4581 4954 1563
Size: 3018 373 3063
Fragments arranged by size:
3063 3018 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 2519 2612 3625 4517 5901 6169 1632
Size: 710 177 93 1013 892 1384 268 1917
Fragments arranged by size:
1917 1384 1013 892 710 268 177 93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6257
AflIII A'CryG_T
Cuts at: 1203 2347 2436 2809 5353 6330 1203
Size: 1144 89 373 2544 977 1327
Fragments arranged by size:
2544 1327 1144 977 373 89
AgeI A'CCGG_T
Cuts at: 5285 5285
Size: 6454
AhdI GACnn_n'nnGTC
Cuts at: 5183 5183
Size: 6454
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3099 1184
Size: 1915 4539
AlwNI CAG_nnn'CTG
Cuts at: 2365 2650 3451 4197 5769 2365
Size: 285 801 746 1572 3050
Fragments arranged by size:
3050 1572 801 746 285
ApaLI G'TGCA_C
Cuts at: 4511 5667 4511
Size: 1156 5298
ApoI r'AATT_y
Cuts at: 1493 2258 2713 3318 3763 3989 4141 6250 1493
Size: 765 455 605 445 226 152 2109 1697
Fragments arranged by size:
2109 1697 765 605 455 445 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5008 160
Size: 872 3976 1606
Fragments arranged by size:
3976 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
AvaI C'yCGr_G
Cuts at: 1870 1943 3779 1870
Size: 73 1836 4545
Fragments arranged by size:
4545 1836 73
BamHI G'GATC_C
Cuts at: 3243 3784 4151 3243
Size: 541 367 5546
Fragments arranged by size:
5546 541 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2545 3542 3590 3775
Size: 325 217 627 221 537 997 48 185
Cuts at: 3775 5230 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 997 627 537 325 221 217
185 48
BanII G_rGCy'C
Cuts at: 1391 3773 1391
Size: 2382 4072
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 6454
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2657 2904 928
Size: 971 758 247 4478
Fragments arranged by size:
4478 971 758 247
BbvCI CC'TCA_GC
Cuts at: 3400 3400
Size: 6454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4645 4679 1903
Size: 34 2708 34 3678
Fragments arranged by size:
3678 2708 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3727 4346 5556 3727
Size: 619 1210 4625
Fragments arranged by size:
4625 1210 619
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6454
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5065 136
Size: 107 122 71 631 605 3393 1525
Fragments arranged by size:
3393 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6449 6449
Size: 6454
BlpI GC'TnA_GC
Cuts at: 3871 3871
Size: 6454
Bme1580I G_kGCm'C
Cuts at: 1790 2548 2568 3494 4515 5671 1790
Size: 758 20 926 1021 1156 2573
Fragments arranged by size:
2573 1156 1021 926 758 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2806 4185 5143
Size: 416 53 83 399 24 1389 1379 958
Cuts at: 5143 442
Size: 1753
Fragments arranged by size:
1753 1389 1379 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2931 3599 3710 5114 1239
Size: 1692 668 111 1404 2579
Fragments arranged by size:
2579 1692 1404 668 111
Bpu10I CC'TnA_GC
Cuts at: 3400 3495 3657 3400
Size: 95 162 6197
Fragments arranged by size:
6197 162 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3391 3445 4579 5444 5742 3391
Size: 54 1134 865 298 4103
Fragments arranged by size:
4103 1134 865 298 54
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5117 882
Size: 617 3618 2219
Fragments arranged by size:
3618 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4245 493
Size: 711 3041 2702
Fragments arranged by size:
3041 2702 711
BsaBI GATnn'nnATC
Cuts at: 6448 6448
Size: 6454
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4261 4643 5278
Size: 53 83 186 936 476 2252 382 635
Cuts at: 5278 275
Size: 1451
Fragments arranged by size:
2252 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4887 5285 5559 5706 6117 4887
Size: 398 274 147 411 5224
Fragments arranged by size:
5224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3193 3223 1178
Size: 30 165 30 1790 30 4409
Fragments arranged by size:
4409 1790 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2045 2576 3596 1186
Size: 856 3 531 1020 4044
Fragments arranged by size:
4044 1020 856 531 3
BseYI C'CCAG_C
Cuts at: 5657 6153 5657
Size: 496 5958
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3543 1163
Size: 573 1807 4074
Fragments arranged by size:
4074 1807 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3904 4665 5693 1540
Size: 275 186 1903 761 1028 2301
Fragments arranged by size:
2301 1903 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 3475 4515 4600 5671 2151
Size: 1324 1040 85 1071 2934
Fragments arranged by size:
2934 1324 1071 1040 85
BsmI GAATG_Cn'
Cuts at: 3088 3965 4058 3088
Size: 877 93 5484
Fragments arranged by size:
5484 877 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2548 2568 3475 3494 3773 4515
Size: 399 361 397 20 907 19 279 742
Cuts at: 4515 4600 5671 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 907 742 399 397 361 279
85 20 19
BspEI T'CCGG_A
Cuts at: 6117 6117
Size: 6454
BspHI T'CATG_A
Cuts at: 2221 3171 4343 6073 2221
Size: 950 1172 1730 2602
Fragments arranged by size:
2602 1730 1172 950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2502 3741 3809 844
Size: 1658 1239 68 3489
Fragments arranged by size:
3489 1658 1239 68
BsrBI CCG'CTC
Cuts at: 1394 1823 4341 1394
Size: 429 2518 3507
Fragments arranged by size:
3507 2518 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2298 3514 4949 5123 65
Size: 1183 1050 1216 1435 174 1396
Fragments arranged by size:
1435 1396 1216 1183 1050 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5098 5285 1700
Size: 110 3288 187 2869
Fragments arranged by size:
3288 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6454
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6454
BssSI C'ACGA_G
Cuts at: 4514 5526 4514
Size: 1012 5442
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2650 794
Size: 1856 4598
BstBI TT'CG_AA
Cuts at: 5258 5258
Size: 6454
BstEII G'GTnAC_C
Cuts at: 1184 3352 1184
Size: 2168 4286
BstXI CCAn_nnnn'nTGG
Cuts at: 3061 3061
Size: 6454
BstYI r'GATC_y
Cuts at: 1972 2244 2470 3243 3784 4151 4536 4553 6005
Size: 272 226 773 541 367 385 17 1452
Cuts at: 6005 6091 6103 6449 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 773 541 385 367 346 272
226 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3741 513
Size: 552 939 1737 3226
Fragments arranged by size:
3226 1737 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5715
Fragments arranged by size:
5715 683 56
BtsI GCAGTG_nn'
Cuts at: 3494 3526 3970 4764 4784 3494
Size: 32 444 794 20 5164
Fragments arranged by size:
5164 794 444 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6454
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3166 3201 529
Size: 35 2602 35 3782
Fragments arranged by size:
3782 2602 35 35
DraI TTT'AAA
Cuts at: 3758 3758
Size: 6454
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3389 5461 809
Size: 507 2073 2072 1802
Fragments arranged by size:
2073 2072 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2740 3025 3211
Size: 54 1425 50 275 18 910 285 186
Cuts at: 3211 3377 3724 3738 3901 4790 6419 8
Size: 166 347 14 163 889 1629 43
Fragments arranged by size:
1629 1425 910 889 347 285 275 186
166 163 54 50 43 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 3901 1537
Size: 275 2089 4090
Fragments arranged by size:
4090 2089 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2238 4384 1353
Size: 699 186 2146 3423
Fragments arranged by size:
3423 2146 699 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5041 5415 5561 387
Size: 1064 138 3452 374 146 1280
Fragments arranged by size:
3452 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2519 2612 2931 3599 3625 3710
Size: 393 710 177 93 319 668 26 85
Cuts at: 3710 4517 5114 5901 6169 1239
Size: 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 710 668 597 393 319
268 177 93 85 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2498 2591 2729 2498
Size: 93 138 6223
Fragments arranged by size:
6223 138 93
EcoO109I rG'GnC_Cy
Cuts at: 2281 2819 3104 3627 3636 2281
Size: 538 285 523 9 5099
Fragments arranged by size:
5099 538 523 285 9
EcoRI G'AATT_C
Cuts at: 3763 4141 3763
Size: 378 6076
FspI TGC'GCA
Cuts at: 4960 4960
Size: 6454
HaeII r_GCGC'y
Cuts at: 1678 2012 5601 1678
Size: 334 3589 2531
Fragments arranged by size:
3589 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2786 2818 5095 5127 5169
Size: 32 135 32 1214 32 2277 32 42
Cuts at: 5169 5201 1373
Size: 32 2626
Fragments arranged by size:
2626 2277 1214 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3798 4044 669
Size: 988 281 1860 246 3079
Fragments arranged by size:
3079 1860 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6454
HpaI GTT'AAC
Cuts at: 4044 4044
Size: 6454
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 6454
KpnI G_GTAC'C
Cuts at: 3546 3779 3546
Size: 233 6221
MfeI C'AATT_G
Cuts at: 4053 4053
Size: 6454
MluI A'CGCG_T
Cuts at: 6330 6330
Size: 6454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2368 5568 5752 1305
Size: 1063 3200 184 2007
Fragments arranged by size:
3200 2007 1063 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3027 3213 3379 3726 10
Size: 54 1425 343 1195 186 166 347 2738
Fragments arranged by size:
2738 1425 1195 347 343 186 166 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2551 3519 4412 4771 4930 518
Size: 648 1385 968 893 359 159 2042
Fragments arranged by size:
2042 1385 968 893 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 3577 3870 4547 5695 5940 1606
Size: 492 1479 293 677 1148 245 2120
Fragments arranged by size:
2120 1479 1148 677 492 293 245
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 6454
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4963
Fragments arranged by size:
4963 939 552
NdeI CA'TA_TG
Cuts at: 387 2552 387
Size: 2165 4289
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6454
NotI GC'GGCC_GC
Cuts at: 3901 3901
Size: 6454
NspI r_CATG'y
Cuts at: 1132 1229 2351 2440 2813 3812 5357 1132
Size: 97 1122 89 373 999 1545 2229
Fragments arranged by size:
2229 1545 1122 999 373 97 89
PasI CC'CwG_GG
Cuts at: 1348 2655 1348
Size: 1307 5147
PciI A'CATG_T
Cuts at: 2347 2436 2809 5353 2347
Size: 89 373 2544 3448
Fragments arranged by size:
3448 2544 373 89
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 2845 3386 1108
Size: 1242 495 541 4176
Fragments arranged by size:
4176 1242 541 495
PfoI T'CCnGG_A
Cuts at: 2604 2947 3687 2604
Size: 343 740 5371
Fragments arranged by size:
5371 740 343
PmeI GTTT'AAAC
Cuts at: 3758 3758
Size: 6454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4523 4555 6036 6068 4523
Size: 32 1481 32 4909
Fragments arranged by size:
4909 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2281 2819 3104 3627 2281
Size: 538 285 523 5108
Fragments arranged by size:
5108 538 523 285
PshAI GACnn'nnGTC
Cuts at: 1613 2904 1613
Size: 1291 5163
PsiI TTA'TAA
Cuts at: 3300 4024 3300
Size: 724 5730
PstI C_TGCA'G
Cuts at: 830 3806 830
Size: 2976 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6454
PvuII CAG'CTG
Cuts at: 3577 3577
Size: 6454
SalI G'TCGA_C
Cuts at: 1936 3796 1936
Size: 1860 4594
SanDI GG'GwC_CC
Cuts at: 2281 2281
Size: 6454
SbfI CC_TGCA'GG
Cuts at: 3806 3806
Size: 6454
ScaI AGT'ACT
Cuts at: 3218 3665 4702 6414 3218
Size: 447 1037 1712 3258
Fragments arranged by size:
3258 1712 1037 447
SfcI C'TryA_G
Cuts at: 826 1045 2176 3802 4937 5618 5809 826
Size: 219 1131 1626 1135 681 191 1471
Fragments arranged by size:
1626 1471 1135 1131 681 219 191
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 6454
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6454
SmaI CCC'GGG
Cuts at: 1872 3781 1872
Size: 1909 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3370 3424 4558 5459 5721 820
Size: 197 926 1427 54 1134 901 262 1553
Fragments arranged by size:
1553 1427 1134 926 901 262 197 54
SnaBI TAC'GTA
Cuts at: 493 4245 493
Size: 3752 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6454
SphI G_CATG'C
Cuts at: 1132 3812 1132
Size: 2680 3774
SspI AAT'ATT
Cuts at: 5 52 2622 4378 5
Size: 47 2570 1756 2081
Fragments arranged by size:
2570 2081 1756 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6454
StyI C'CwwG_G
Cuts at: 513 1065 2004 3893 513
Size: 552 939 1889 3074
Fragments arranged by size:
3074 1889 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2563 2669 2760 3517 4481 4498 4651
Size: 1255 983 106 91 757 964 17 153
Cuts at: 4651 4836 5281 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 983 964 757 445 185 153
106 91 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 2806 3216
Size: 274 80 33 51 1443 105 724 410
Cuts at: 3216 3663 4700 6412 96
Size: 447 1037 1712 138
Fragments arranged by size:
1712 1443 1037 724 447 410 274 138
105 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3088 3120 3193 3225 6414 6446 3088
Size: 32 73 32 3189 32 3096
Fragments arranged by size:
3189 3096 73 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2600 3613 1316
Size: 1284 1013 4157
Fragments arranged by size:
4157 1284 1013
XbaI T'CTAG_A
Cuts at: 3790 3790
Size: 6454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2842 3277 2842
Size: 435 6019
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6454
XmaI C'CCGG_G
Cuts at: 1870 3779 1870
Size: 1909 4545
XmnI GAAnn'nnTTC
Cuts at: 2232 2901 4583 2232
Size: 669 1682 4103
Fragments arranged by size:
4103 1682 669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4262 276
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI