Restriction map for
pFN21AE5639
(Circular) (Six-base) MAPSORT of: pFN21AE5639.seq Check: 2290 from: 1 to: 6391
pFN21AE5639 6391 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2530 4201 278
Size: 53 83 186 936 994 1671 2468
Fragments arranged by size:
2468 1671 994 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3734 1937
Size: 1797 4594
Acc65I G'GTAC_C
Cuts at: 3712 3712
Size: 6391
AclI AA'CG_TT
Cuts at: 1563 4518 4891 1563
Size: 2955 373 3063
Fragments arranged by size:
3063 2955 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2708 3090 3273 3324 4454 5838 6106 1632
Size: 1076 382 183 51 1130 1384 268 1917
Fragments arranged by size:
1917 1384 1130 1076 382 268 183 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6194
AflIII A'CryG_T
Cuts at: 1203 5290 6267 1203
Size: 4087 977 1327
Fragments arranged by size:
4087 1327 977
AgeI A'CCGG_T
Cuts at: 5222 5222
Size: 6391
AhdI GACnn_n'nnGTC
Cuts at: 5120 5120
Size: 6391
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6391
AlwNI CAG_nnn'CTG
Cuts at: 2156 2176 2395 2417 2647 2770 2854 2971 3001
Size: 20 219 22 230 123 84 117 30
Cuts at: 3001 3581 4134 5706 2156
Size: 580 553 1572 2841
Fragments arranged by size:
2841 1572 580 553 230 219 123 117
84 30 22 20
ApaI G_GGCC'C
Cuts at: 2024 2210 2267 3057 3079 2024
Size: 186 57 790 22 5336
Fragments arranged by size:
5336 790 186 57 22
ApaLI G'TGCA_C
Cuts at: 4448 5604 4448
Size: 1156 5235
ApoI r'AATT_y
Cuts at: 1493 3700 3926 4078 6187 1493
Size: 2207 226 152 2109 1697
Fragments arranged by size:
2207 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4945 160
Size: 872 3913 1606
Fragments arranged by size:
3913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
AvaI C'yCGr_G
Cuts at: 1870 1943 2465 3716 1870
Size: 73 522 1251 4545
Fragments arranged by size:
4545 1251 522 73
BamHI G'GATC_C
Cuts at: 3721 4088 3721
Size: 367 6024
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3475 3712 5167 618
Size: 325 217 627 1688 237 1455 1842
Fragments arranged by size:
1842 1688 1455 627 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2024 2210 2267 2380 2517 3057 3079 3406
Size: 633 186 57 113 137 540 22 327
Cuts at: 3406 3710 1391
Size: 304 4072
Fragments arranged by size:
4072 633 540 327 304 186 137 113
57 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2460 2696 928
Size: 971 561 236 4623
Fragments arranged by size:
4623 971 561 236
BbvCI CC'TCA_GC
Cuts at: 2418 2574 2418
Size: 156 6235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4582 4616 1903
Size: 34 2645 34 3678
Fragments arranged by size:
3678 2645 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4283 5493 4283
Size: 1210 5181
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6391
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2747 5002 136
Size: 107 122 71 631 605 1075 2255 1525
Fragments arranged by size:
2255 1525 1075 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2604 6386 2604
Size: 3782 2609
BlpI GC'TnA_GC
Cuts at: 2492 2516 3808 2492
Size: 24 1292 5075
Fragments arranged by size:
5075 1292 24
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2210 2267 3057 3079 4452 5608 1790
Size: 234 186 57 790 22 1373 1156 2573
Fragments arranged by size:
2573 1373 1156 790 234 186 57 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3494 4122 5080
Size: 416 53 83 399 24 2077 628 958
Cuts at: 5080 442
Size: 1753
Fragments arranged by size:
2077 1753 958 628 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2456 2522 2531 2699 2729 3029 3044
Size: 968 249 66 9 168 30 300 15
Cuts at: 3044 3420 3435 3446 3644 3659 3669 5051 1239
Size: 376 15 11 198 15 10 1382 2579
Fragments arranged by size:
2579 1382 968 376 300 249 198 168
66 30 15 15 15 11 10 9
Bpu10I CC'TnA_GC
Cuts at: 2418 2574 2418
Size: 156 6235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4516 5381 5679 4516
Size: 865 298 5228
Fragments arranged by size:
5228 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2354 3218 3436 5054 882
Size: 617 855 864 218 1618 2219
Fragments arranged by size:
2219 1618 864 855 617 218
BsaAI yAC'GTr
Cuts at: 493 1204 4182 493
Size: 711 2978 2702
Fragments arranged by size:
2978 2702 711
BsaBI GATnn'nnATC
Cuts at: 6385 6385
Size: 6391
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2527 4198 4580 5215
Size: 53 83 186 936 994 1671 382 635
Cuts at: 5215 275
Size: 1451
Fragments arranged by size:
1671 1451 994 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4824 5222 5496 5643 6054 4824
Size: 398 274 147 411 5161
Fragments arranged by size:
5161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6166
Fragments arranged by size:
6166 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2117 2372 2435 2591 2615 2687 2813
Size: 898 33 255 63 156 24 72 126
Cuts at: 2813 3041 3368 3523 1186
Size: 228 327 155 4054
Fragments arranged by size:
4054 898 327 255 228 156 155 126
72 63 33 24
BseYI C'CCAG_C
Cuts at: 2080 2388 2637 3078 3121 3259 3263 3574 5594
Size: 308 249 441 43 138 4 311 2020
Cuts at: 5594 6090 2080
Size: 496 2381
Fragments arranged by size:
2381 2020 496 441 311 308 249 138
43 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5818
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3841 4602 5630 1540
Size: 275 186 1840 761 1028 2301
Fragments arranged by size:
2301 1840 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2517 4452 4537 5608 2517
Size: 1935 85 1071 3300
Fragments arranged by size:
3300 1935 1071 85
BsmI GAATG_Cn'
Cuts at: 2091 2548 3902 3995 2091
Size: 457 1354 93 4487
Fragments arranged by size:
4487 1354 457 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2210 2267 2380 2517 3057 3079
Size: 399 234 186 57 113 137 540 22
Cuts at: 3079 3406 3710 4452 4537 5608 1391
Size: 327 304 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 540 399 327 304 234
186 137 113 85 57 22
BspEI T'CCGG_A
Cuts at: 6054 6054
Size: 6391
BspHI T'CATG_A
Cuts at: 4280 6010 4280
Size: 1730 4661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2019 2496 3454 3746 844
Size: 1175 477 958 292 3489
Fragments arranged by size:
3489 1175 958 477 292
BsrBI CCG'CTC
Cuts at: 1394 1823 2062 2824 4278 1394
Size: 429 239 762 1454 3507
Fragments arranged by size:
3507 1454 762 429 239
BsrDI GCAATG_nn'
Cuts at: 65 1248 4886 5060 65
Size: 1183 3638 174 1396
Fragments arranged by size:
3638 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2089 5035 5222 1700
Size: 110 279 2946 187 2869
Fragments arranged by size:
2946 2869 279 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6391
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6391
BssSI C'ACGA_G
Cuts at: 4451 5463 4451
Size: 1012 5379
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2647 794
Size: 1853 4538
BstBI TT'CG_AA
Cuts at: 2127 5195 2127
Size: 3068 3323
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6391
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2665 2707 3330 2245
Size: 420 42 623 5306
Fragments arranged by size:
5306 623 420 42
BstYI r'GATC_y
Cuts at: 1972 2604 3721 4088 4473 4490 5942 6028 6040
Size: 632 1117 367 385 17 1452 86 12
Cuts at: 6040 6386 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1117 632 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2016 2067 2268 2016
Size: 51 201 6139
Fragments arranged by size:
6139 201 51
BtgI C'CryG_G
Cuts at: 513 1065 2004 2664 2859 513
Size: 552 939 660 195 4045
Fragments arranged by size:
4045 939 660 552 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2961 3102 487
Size: 683 56 1735 141 3776
Fragments arranged by size:
3776 1735 683 141 56
BtsI GCAGTG_nn'
Cuts at: 2037 3209 3907 4701 4721 2037
Size: 1172 698 794 20 3707
Fragments arranged by size:
3707 1172 794 698 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6356
DraI TTT'AAA
Cuts at: 3695 3695
Size: 6391
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5398 809
Size: 507 4082 1802
Fragments arranged by size:
4082 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 2697 2856
Size: 54 1425 50 275 18 405 462 159
Cuts at: 2856 3838 4727 6356 8
Size: 982 889 1629 43
Fragments arranged by size:
1629 1425 982 889 462 405 275 159
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3838 1537
Size: 275 2026 4090
Fragments arranged by size:
4090 2026 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2349 3082 3337 3345 4321 1353
Size: 996 733 255 8 976 3423
Fragments arranged by size:
3423 996 976 733 255 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2765 4978 5352 5498 387
Size: 1064 138 1176 2213 374 146 1280
Fragments arranged by size:
2213 1280 1176 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2515 2515
Size: 6391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2456 2522 2531 2699 2708 2729
Size: 393 575 249 66 9 168 9 21
Cuts at: 2729 3029 3044 3090 3273 3324 3420 3435 3446
Size: 300 15 46 183 51 96 15 11
Cuts at: 3446 3644 3659 3669 4454 5051 5838 6106 1239
Size: 198 15 10 785 597 787 268 1524
Fragments arranged by size:
1524 787 785 597 575 393 300 268
249 198 183 168 96 66 51 46
21 15 15 15 11 10 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 2597 3306 2015
Size: 582 709 5100
Fragments arranged by size:
5100 709 582
EcoO109I rG'GnC_Cy
Cuts at: 2020 2206 2207 2263 2264 2506 2644 2683 3053
Size: 186 1 56 1 242 138 39 370
Cuts at: 3053 3075 3513 2020
Size: 22 438 4898
Fragments arranged by size:
4898 438 370 242 186 138 56 39
22 1 1
EcoRI G'AATT_C
Cuts at: 3700 4078 3700
Size: 378 6013
FspI TGC'GCA
Cuts at: 4897 4897
Size: 6391
HaeII r_GCGC'y
Cuts at: 1678 5538 1678
Size: 3860 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2567 2596 2599 2628 2773
Size: 32 135 32 995 29 3 29 145
Cuts at: 2773 2805 2910 2942 3233 3265 3562 3594 5032
Size: 32 105 32 291 32 297 32 1438
Cuts at: 5032 5064 5106 5138 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1438 995 297 291 145 135 105
42 32 32 32 32 32 32 32
32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2330 3336 3735 3981 669
Size: 988 281 392 1006 399 246 3079
Fragments arranged by size:
3079 1006 988 399 392 281 246
HindIII A'AGCT_T
Cuts at: 748 2130 748
Size: 1382 5009
HpaI GTT'AAC
Cuts at: 3981 3981
Size: 6391
KpnI G_GTAC'C
Cuts at: 3716 3716
Size: 6391
MfeI C'AATT_G
Cuts at: 3990 3990
Size: 6391
MluI A'CGCG_T
Cuts at: 6267 6267
Size: 6391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3204 5505 5689 1305
Size: 1899 2301 184 2007
Fragments arranged by size:
2301 2007 1899 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 2699 2858 10
Size: 54 1425 343 405 462 159 3543
Fragments arranged by size:
3543 1425 462 405 343 159 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2404 3328 4349 4708 4867 518
Size: 648 1238 924 1021 359 159 2042
Fragments arranged by size:
2042 1238 1021 924 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2483 2557 2573 3034 3082 3263 3544 3807
Size: 877 74 16 461 48 181 281 263
Cuts at: 3807 4484 5632 5877 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 877 677 461 281 263 245
181 74 48 16
NcoI C'CATG_G
Cuts at: 513 1065 2004 2664 2859 513
Size: 552 939 660 195 4045
Fragments arranged by size:
4045 939 660 552 195
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6391
NotI GC'GGCC_GC
Cuts at: 3838 3838
Size: 6391
NruI TCG'CGA
Cuts at: 2946 2946
Size: 6391
NspI r_CATG'y
Cuts at: 1132 1229 3749 5294 1132
Size: 97 2520 1545 2229
Fragments arranged by size:
2520 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6391
PciI A'CATG_T
Cuts at: 5290 5290
Size: 6391
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2176 2233 2971 1108
Size: 1068 57 738 4528
Fragments arranged by size:
4528 1068 738 57
PfoI T'CCnGG_A
Cuts at: 2055 2055
Size: 6391
PmeI GTTT'AAAC
Cuts at: 3695 3695
Size: 6391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4460 4492 5973 6005 4460
Size: 32 1481 32 4846
Fragments arranged by size:
4846 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2506 2683 2506
Size: 177 6214
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6391
PsiI TTA'TAA
Cuts at: 3961 3961
Size: 6391
PspOMI G'GGCC_C
Cuts at: 2020 2206 2263 3053 3075 2020
Size: 186 57 790 22 5336
Fragments arranged by size:
5336 790 186 57 22
PstI C_TGCA'G
Cuts at: 830 2556 2652 2796 3743 830
Size: 1726 96 144 947 3478
Fragments arranged by size:
3478 1726 947 144 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6391
PvuII CAG'CTG
Cuts at: 2483 2573 3034 3082 3263 2483
Size: 90 461 48 181 5611
Fragments arranged by size:
5611 461 181 90 48
SacI G_AGCT'C
Cuts at: 2517 2517
Size: 6391
SalI G'TCGA_C
Cuts at: 1936 3733 1936
Size: 1797 4594
SbfI CC_TGCA'GG
Cuts at: 3743 3743
Size: 6391
ScaI AGT'ACT
Cuts at: 4639 6351 4639
Size: 1712 4679
SfcI C'TryA_G
Cuts at: 826 1045 2552 2648 2792 3739 4874 5555 5746
Size: 219 1507 96 144 947 1135 681 191
Cuts at: 5746 826
Size: 1471
Fragments arranged by size:
1507 1471 1135 947 681 219 191 144
96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6391
SmaI CCC'GGG
Cuts at: 1872 3718 1872
Size: 1846 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4495 5396 5658 820
Size: 197 926 2552 901 262 1553
Fragments arranged by size:
2552 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4182 493
Size: 3689 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6391
SphI G_CATG'C
Cuts at: 1132 3749 1132
Size: 2617 3774
SspI AAT'ATT
Cuts at: 5 52 4315 5
Size: 47 4263 2081
Fragments arranged by size:
4263 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6391
StyI C'CwwG_G
Cuts at: 513 1065 2004 2593 2658 2664 2859 3830 513
Size: 552 939 589 65 6 195 971 3074
Fragments arranged by size:
3074 971 939 589 552 195 65 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2018 2651 3116 4418 4435 4588 4773
Size: 1255 438 633 465 1302 17 153 185
Cuts at: 4773 5218 325
Size: 445 1498
Fragments arranged by size:
1498 1302 1255 633 465 445 438 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4637 6349 96
Size: 274 80 33 51 1443 2660 1712 138
Fragments arranged by size:
2660 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3547 3579 6351 6383 3547
Size: 32 2772 32 3555
Fragments arranged by size:
3555 2772 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6391
XbaI T'CTAG_A
Cuts at: 3727 3727
Size: 6391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 3645 2281
Size: 1364 5027
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6391
XmaI C'CCGG_G
Cuts at: 1870 3716 1870
Size: 1846 4545
XmnI GAAnn'nnTTC
Cuts at: 4520 4520
Size: 6391
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2528 4199 276
Size: 53 83 186 936 994 1671 2468
Fragments arranged by size:
2468 1671 994 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI