Restriction map for
pFN21AE5638
(Circular) (Six-base) MAPSORT of: pFN21AE5638.seq Check: 6523 from: 1 to: 6346
pFN21AE5638 6346 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4156 278
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3689 1937
Size: 1752 4594
Acc65I G'GTAC_C
Cuts at: 3667 3667
Size: 6346
AclI AA'CG_TT
Cuts at: 1563 4473 4846 1563
Size: 2910 373 3063
Fragments arranged by size:
3063 2910 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2568 2805 3048 3605 4409 5793 6061 1632
Size: 936 237 243 557 804 1384 268 1917
Fragments arranged by size:
1917 1384 936 804 557 268 243 237
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6149
AflIII A'CryG_T
Cuts at: 1203 2317 5245 6222 1203
Size: 1114 2928 977 1327
Fragments arranged by size:
2928 1327 1114 977
AgeI A'CCGG_T
Cuts at: 5177 5177
Size: 6346
AhdI GACnn_n'nnGTC
Cuts at: 5075 5075
Size: 6346
AleI CACnn'nnGTG
Cuts at: 1166 2585 1166
Size: 1419 4927
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6346
AlwNI CAG_nnn'CTG
Cuts at: 2065 4089 5661 2065
Size: 2024 1572 2750
Fragments arranged by size:
2750 2024 1572
ApaI G_GGCC'C
Cuts at: 3004 3004
Size: 6346
ApaLI G'TGCA_C
Cuts at: 2198 4403 5559 2198
Size: 2205 1156 2985
Fragments arranged by size:
2985 2205 1156
ApoI r'AATT_y
Cuts at: 1493 2173 3500 3655 3881 4033 6142 1493
Size: 680 1327 155 226 152 2109 1697
Fragments arranged by size:
2109 1697 1327 680 226 155 152
AseI AT'TA_AT
Cuts at: 160 1032 4900 160
Size: 872 3868 1606
Fragments arranged by size:
3868 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
AvaI C'yCGr_G
Cuts at: 1870 1943 3671 1870
Size: 73 1728 4545
Fragments arranged by size:
4545 1728 73
AvrII C'CTAG_G
Cuts at: 3571 3571
Size: 6346
BamHI G'GATC_C
Cuts at: 3446 3676 4043 3446
Size: 230 367 5749
Fragments arranged by size:
5749 367 230
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3667 5122 618
Size: 325 217 627 1880 1455 1842
Fragments arranged by size:
1880 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2747 2799 3004 3665 1391
Size: 1356 52 205 661 4072
Fragments arranged by size:
4072 1356 661 205 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2634 2718 3101 928
Size: 971 735 84 383 4173
Fragments arranged by size:
4173 971 735 383 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4537 4571 1903
Size: 34 2600 34 3678
Fragments arranged by size:
3678 2600 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2197 4238 5448 2197
Size: 2041 1210 3095
Fragments arranged by size:
3095 2041 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6346
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3008 4957 136
Size: 107 122 71 631 605 1336 1949 1525
Fragments arranged by size:
1949 1525 1336 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6341 6341
Size: 6346
BlpI GC'TnA_GC
Cuts at: 3763 3763
Size: 6346
Bme1580I G_kGCm'C
Cuts at: 1790 2202 3004 3028 3259 4407 5563 1790
Size: 412 802 24 231 1148 1156 2573
Fragments arranged by size:
2573 1156 1148 802 412 231 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6346
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4077 5035 442
Size: 416 53 83 399 24 2660 958 1753
Fragments arranged by size:
2660 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 2757 5006 1239
Size: 1001 517 2249 2579
Fragments arranged by size:
2579 2249 1001 517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2703 3608 4471 5336 5634 2703
Size: 905 863 865 298 3415
Fragments arranged by size:
3415 905 865 863 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5009 882
Size: 617 3510 2219
Fragments arranged by size:
3510 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4137 493
Size: 711 2933 2702
Fragments arranged by size:
2933 2702 711
BsaBI GATnn'nnATC
Cuts at: 6340 6340
Size: 6346
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3098 4153 4535 5170
Size: 53 83 186 936 1565 1055 382 635
Cuts at: 5170 275
Size: 1451
Fragments arranged by size:
1565 1451 1055 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4779 5177 5451 5598 6009 4779
Size: 398 274 147 411 5116
Fragments arranged by size:
5116 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6121
Fragments arranged by size:
6121 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2642 3059 1186
Size: 1456 417 4473
Fragments arranged by size:
4473 1456 417
BseYI C'CCAG_C
Cuts at: 2065 2493 2525 2645 5549 6045 2065
Size: 428 32 120 2904 496 2366
Fragments arranged by size:
2904 2366 496 428 120 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2541 1163
Size: 573 805 4968
Fragments arranged by size:
4968 805 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3796 4557 5585 1540
Size: 275 186 1795 761 1028 2301
Fragments arranged by size:
2301 1795 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2202 4407 4492 5563 2202
Size: 2205 85 1071 2985
Fragments arranged by size:
2985 2205 1071 85
BsmI GAATG_Cn'
Cuts at: 2623 3462 3857 3950 2623
Size: 839 395 93 5019
Fragments arranged by size:
5019 839 395 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2747 2799 3004 3028 3259 3665
Size: 399 412 545 52 205 24 231 406
Cuts at: 3665 4407 4492 5563 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 545 412 406 399 231
205 85 52 24
BspEI T'CCGG_A
Cuts at: 6009 6009
Size: 6346
BspHI T'CATG_A
Cuts at: 3222 4235 5965 3222
Size: 1013 1730 3603
Fragments arranged by size:
3603 1730 1013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2280 3701 844
Size: 1436 1421 3489
Fragments arranged by size:
3489 1436 1421
BsrBI CCG'CTC
Cuts at: 1394 1823 4233 1394
Size: 429 2410 3507
Fragments arranged by size:
3507 2410 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4841 5015 65
Size: 1183 3593 174 1396
Fragments arranged by size:
3593 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4990 5177 1700
Size: 110 3180 187 2869
Fragments arranged by size:
3180 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2145 96
Size: 2049 4297
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6346
BssSI C'ACGA_G
Cuts at: 4406 5418 4406
Size: 1012 5334
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2053 794
Size: 1259 5087
BstBI TT'CG_AA
Cuts at: 5150 5150
Size: 6346
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6346
BstYI r'GATC_y
Cuts at: 1972 2508 3446 3676 4043 4428 4445 5897 5983
Size: 536 938 230 367 385 17 1452 86
Cuts at: 5983 5995 6341 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 938 536 385 367 346 230
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2025 2985 513
Size: 552 960 960 3874
Fragments arranged by size:
3874 960 960 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2517 487
Size: 683 56 1291 4316
Fragments arranged by size:
4316 1291 683 56
BtsI GCAGTG_nn'
Cuts at: 3862 4656 4676 3862
Size: 794 20 5532
Fragments arranged by size:
5532 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6311
DraI TTT'AAA
Cuts at: 3650 3650
Size: 6346
DraIII CAC_nnn'GTG
Cuts at: 2459 2459
Size: 6346
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5353 809
Size: 507 4037 1802
Fragments arranged by size:
4037 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2673 3793 4682
Size: 54 1425 50 275 18 843 1120 889
Cuts at: 4682 6311 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1120 889 843 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3793 1537
Size: 275 1981 4090
Fragments arranged by size:
4090 1981 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2025 2247 2631 2787 3337 3581 4276 1353
Size: 672 222 384 156 550 244 695 3423
Fragments arranged by size:
3423 695 672 550 384 244 222 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4933 5307 5453 387
Size: 1064 138 3344 374 146 1280
Fragments arranged by size:
3344 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2568 2757 2805 3048 3605 4409
Size: 393 608 328 189 48 243 557 804
Cuts at: 4409 5006 5793 6061 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 804 787 608 597 557 393 328
268 243 189 48
EcoO109I rG'GnC_Cy
Cuts at: 3000 3087 3000
Size: 87 6259
EcoRI G'AATT_C
Cuts at: 3655 4033 3655
Size: 378 5968
EcoRV GAT'ATC
Cuts at: 3298 3298
Size: 6346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2054 2598 2054
Size: 544 5802
FspI TGC'GCA
Cuts at: 4852 4852
Size: 6346
HaeII r_GCGC'y
Cuts at: 1678 2420 5493 1678
Size: 742 3073 2531
Fragments arranged by size:
3073 2531 742
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2904 2936 3585 3617 4987
Size: 32 135 32 1332 32 649 32 1370
Cuts at: 4987 5019 5061 5093 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1370 1332 649 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2604 2862 3489 3690 3936 669
Size: 988 281 666 258 627 201 246 3079
Fragments arranged by size:
3079 988 666 627 281 258 246 201
HindIII A'AGCT_T
Cuts at: 748 2308 748
Size: 1560 4786
HpaI GTT'AAC
Cuts at: 2604 3489 3936 2604
Size: 885 447 5014
Fragments arranged by size:
5014 885 447
KpnI G_GTAC'C
Cuts at: 3671 3671
Size: 6346
MfeI C'AATT_G
Cuts at: 3945 3945
Size: 6346
MluI A'CGCG_T
Cuts at: 6222 6222
Size: 6346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5460 5644 1305
Size: 4155 184 2007
Fragments arranged by size:
4155 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 10
Size: 54 1425 343 843 3681
Fragments arranged by size:
3681 1425 843 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 4304 4663 4822 518
Size: 648 1419 1719 359 159 2042
Fragments arranged by size:
2042 1719 1419 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3762 4439 5587 5832 1606
Size: 2156 677 1148 245 2120
Fragments arranged by size:
2156 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2025 513
Size: 552 960 4834
Fragments arranged by size:
4834 960 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6346
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6346
NotI GC'GGCC_GC
Cuts at: 3793 3793
Size: 6346
NspI r_CATG'y
Cuts at: 1132 1229 3704 5249 1132
Size: 97 2475 1545 2229
Fragments arranged by size:
2475 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6346
PciI A'CATG_T
Cuts at: 5245 5245
Size: 6346
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2065 3172 1108
Size: 957 1107 4282
Fragments arranged by size:
4282 1107 957
PfoI T'CCnGG_A
Cuts at: 2565 2565
Size: 6346
PmeI GTTT'AAAC
Cuts at: 3650 3650
Size: 6346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4415 4447 5928 5960 4415
Size: 32 1481 32 4801
Fragments arranged by size:
4801 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3087 3087
Size: 6346
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6346
PsiI TTA'TAA
Cuts at: 3916 3916
Size: 6346
PspOMI G'GGCC_C
Cuts at: 3000 3000
Size: 6346
PstI C_TGCA'G
Cuts at: 830 3698 830
Size: 2868 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6346
SalI G'TCGA_C
Cuts at: 1936 3688 1936
Size: 1752 4594
SapI GCTCTTCn'nnn_
Cuts at: 2787 2787
Size: 6346
SbfI CC_TGCA'GG
Cuts at: 3698 3698
Size: 6346
ScaI AGT'ACT
Cuts at: 4594 6306 4594
Size: 1712 4634
SfcI C'TryA_G
Cuts at: 826 1045 2167 3694 4829 5510 5701 826
Size: 219 1122 1527 1135 681 191 1471
Fragments arranged by size:
1527 1471 1135 1122 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6346
SmaI CCC'GGG
Cuts at: 1872 3673 1872
Size: 1801 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2718 3623 4450 5351 5613 820
Size: 197 926 775 905 827 901 262 1553
Fragments arranged by size:
1553 926 905 901 827 775 262 197
SnaBI TAC'GTA
Cuts at: 493 4137 493
Size: 3644 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6346
SphI G_CATG'C
Cuts at: 1132 3704 1132
Size: 2572 3774
SspI AAT'ATT
Cuts at: 5 52 3334 4270 5
Size: 47 3282 936 2081
Fragments arranged by size:
3282 2081 936 47
StuI AGG'CCT
Cuts at: 1517 2112 1517
Size: 595 5751
StyI C'CwwG_G
Cuts at: 513 1065 2025 2739 3571 3785 513
Size: 552 960 714 832 214 3074
Fragments arranged by size:
3074 960 832 714 552 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2228 4373 4390 4543 4728 5173 325
Size: 1255 648 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 648 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 3104 4592
Size: 274 80 33 51 1443 168 959 1488
Cuts at: 4592 6304 96
Size: 1712 138
Fragments arranged by size:
1712 1488 1443 959 274 168 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3438 3470 6306 6338 3438
Size: 32 2836 32 3446
Fragments arranged by size:
3446 2836 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2540 1316
Size: 1224 5122
XbaI T'CTAG_A
Cuts at: 2909 3682 2909
Size: 773 5573
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6346
XmaI C'CCGG_G
Cuts at: 1870 3671 1870
Size: 1801 4545
XmnI GAAnn'nnTTC
Cuts at: 2581 4475 2581
Size: 1894 4452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4154 276
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI