Restriction map for
pFN21AE5637
(Circular) (Six-base) MAPSORT of: pFN21AE5637.seq Check: 2106 from: 1 to: 6217
pFN21AE5637 6217 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 3560 1937
Size: 328 1295 4594
Fragments arranged by size:
4594 1295 328
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2544 3022 3428 4280 5664 5932 1632
Size: 912 478 406 852 1384 268 1917
Fragments arranged by size:
1917 1384 912 852 478 406 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 2354 2997 3339 5116 6093 1203
Size: 1151 643 342 1777 977 1327
Fragments arranged by size:
1777 1327 1151 977 643 342
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 3426 1166
Size: 2260 3957
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 2795 3960 5532 2795
Size: 1165 1572 3480
Fragments arranged by size:
3480 1572 1165
ApaI G_GGCC'C
Cuts at: 3035 3035
Size: 6217
ApaLI G'TGCA_C
Cuts at: 2778 4274 5430 2778
Size: 1496 1156 3565
Fragments arranged by size:
3565 1496 1156
ApoI r'AATT_y
Cuts at: 1493 2472 2537 2551 3526 3752 3904 6013 1493
Size: 979 65 14 975 226 152 2109 1697
Fragments arranged by size:
2109 1697 979 975 226 152 65 14
AseI AT'TA_AT
Cuts at: 160 1032 3128 4771 160
Size: 872 2096 1643 1606
Fragments arranged by size:
2096 1643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 3542 1870
Size: 73 1599 4545
Fragments arranged by size:
4545 1599 73
AvrII C'CTAG_G
Cuts at: 3407 3407
Size: 6217
BamHI G'GATC_C
Cuts at: 2503 3403 3547 3914 2503
Size: 900 144 367 4806
Fragments arranged by size:
4806 900 367 144
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3179 3538 4993 618
Size: 325 217 627 1392 359 1455 1842
Fragments arranged by size:
1842 1455 1392 627 359 325 217
BanII G_rGCy'C
Cuts at: 1391 3035 3536 1391
Size: 1644 501 4072
Fragments arranged by size:
4072 1644 501
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2402 2604 928
Size: 971 503 202 4541
Fragments arranged by size:
4541 971 503 202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3213 3247 4408 4442 1903
Size: 34 1276 34 1161 34 3678
Fragments arranged by size:
3678 1276 1161 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 3154 1548
Size: 1606 4611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4828 136
Size: 107 122 71 631 605 3156 1525
Fragments arranged by size:
3156 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3045 6212 3045
Size: 3167 3050
BlpI GC'TnA_GC
Cuts at: 3634 3634
Size: 6217
Bme1580I G_kGCm'C
Cuts at: 1790 2782 3035 4278 5434 1790
Size: 992 253 1243 1156 2573
Fragments arranged by size:
2573 1243 1156 992 253
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3948 4906 442
Size: 416 53 83 399 24 2531 958 1753
Fragments arranged by size:
2531 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2369 1056
Size: 1313 4904
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2292 4877 1239
Size: 1053 2585 2579
Fragments arranged by size:
2585 2579 1053
Bpu10I CC'TnA_GC
Cuts at: 2703 2703
Size: 6217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4342 5207 5505 4342
Size: 865 298 5054
Fragments arranged by size:
5054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4880 882
Size: 617 3381 2219
Fragments arranged by size:
3381 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4024 4406 5041 275
Size: 53 83 186 936 2491 382 635 1451
Fragments arranged by size:
2491 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5992
Fragments arranged by size:
5992 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6217
BseYI C'CCAG_C
Cuts at: 5420 5916 5420
Size: 496 5721
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2433 2808 1163
Size: 573 697 375 4572
Fragments arranged by size:
4572 697 573 375
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2782 4278 4363 5434 2782
Size: 1496 85 1071 3565
Fragments arranged by size:
3565 1496 1071 85
BsmI GAATG_Cn'
Cuts at: 2095 2461 3728 3821 2095
Size: 366 1267 93 4491
Fragments arranged by size:
4491 1267 366 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2782 3035 3536 4278 4363 5434 1391
Size: 399 992 253 501 742 85 1071 2174
Fragments arranged by size:
2174 1071 992 742 501 399 253 85
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 3021 4106 5836 3021
Size: 1085 1730 3402
Fragments arranged by size:
3402 1730 1085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3572 844
Size: 2728 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4104 1394
Size: 429 2281 3507
Fragments arranged by size:
3507 2281 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2065 2514 4712 4886 65
Size: 1183 817 449 2198 174 1396
Fragments arranged by size:
2198 1396 1183 817 449 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4861 5048 1700
Size: 110 3051 187 2869
Fragments arranged by size:
3051 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2786 2795 794
Size: 1992 9 4216
Fragments arranged by size:
4216 1992 9
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstXI CCAn_nnnn'nTGG
Cuts at: 3400 3400
Size: 6217
BstYI r'GATC_y
Cuts at: 1972 2503 3045 3403 3547 3914 4299 4316 5768
Size: 531 542 358 144 367 385 17 1452
Cuts at: 5768 5854 5866 6212 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 542 531 385 367 358 346
144 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4726
Fragments arranged by size:
4726 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 3733 4527 4547 3733
Size: 794 20 5403
Fragments arranged by size:
5403 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 2245 2542 2713 3521 2245
Size: 297 171 808 4941
Fragments arranged by size:
4941 808 297 171
DraIII CAC_nnn'GTG
Cuts at: 2786 2786
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2581 2758 3390
Size: 54 1425 50 275 18 751 177 632
Cuts at: 3390 3664 4553 6182 8
Size: 274 889 1629 43
Fragments arranged by size:
1629 1425 889 751 632 275 274 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 2187 4147 1353
Size: 720 114 1960 3423
Fragments arranged by size:
3423 1960 720 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4804 5178 5324 387
Size: 1064 138 3215 374 146 1280
Fragments arranged by size:
3215 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2292 2544 3022 3428 4280 4877 5664
Size: 393 660 252 478 406 852 597 787
Cuts at: 5664 5932 1239
Size: 268 1524
Fragments arranged by size:
1524 852 787 660 597 478 406 393
268 252
EcoO109I rG'GnC_Cy
Cuts at: 3032 3032
Size: 6217
EcoRI G'AATT_C
Cuts at: 2472 3526 3904 2472
Size: 1054 378 4785
Fragments arranged by size:
4785 1054 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2636 2636
Size: 6217
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 5364 1678
Size: 3686 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2717 2749 4858
Size: 32 135 32 614 32 499 32 2109
Cuts at: 4858 4890 4932 4964 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2109 614 499 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2926 3561 3807 669
Size: 988 281 988 635 246 3079
Fragments arranged by size:
3079 988 988 635 281 246
HindIII A'AGCT_T
Cuts at: 748 2258 748
Size: 1510 4707
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 2305 2372 2919 5331 5515 1305
Size: 798 202 67 547 2412 184 2007
Fragments arranged by size:
2412 2007 798 547 202 184 67
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2760 3392 10
Size: 54 1425 343 928 632 2835
Fragments arranged by size:
2835 1425 928 632 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2841 3026 3426 4175 4534 4693 518
Size: 648 1675 185 400 749 359 159 2042
Fragments arranged by size:
2042 1675 749 648 400 359 185 159
MspA1I CmG'CkG
Cuts at: 1606 3633 4310 5458 5703 1606
Size: 2027 677 1148 245 2120
Fragments arranged by size:
2120 2027 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4726
Fragments arranged by size:
4726 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 2365 1052
Size: 1313 4904
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 2237 2358 3001 3343 3575 5120 1132
Size: 97 1008 121 643 342 232 1545 2229
Fragments arranged by size:
2229 1545 1008 643 342 232 121 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 2354 2997 3339 5116 2354
Size: 643 342 1777 3455
Fragments arranged by size:
3455 1777 643 342
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2483 3388 1108
Size: 1375 905 3937
Fragments arranged by size:
3937 1375 905
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3445 1613
Size: 1832 4385
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 3031 3031
Size: 6217
PstI C_TGCA'G
Cuts at: 830 3569 830
Size: 2739 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 3565 4700 5381 5572 826
Size: 219 2520 1135 681 191 1471
Fragments arranged by size:
2520 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4321 5222 5484 820
Size: 197 926 2378 901 262 1553
Fragments arranged by size:
2378 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 2146 152
Size: 1994 4223
SphI G_CATG'C
Cuts at: 1132 2237 3575 1132
Size: 1105 1338 3774
Fragments arranged by size:
3774 1338 1105
SspI AAT'ATT
Cuts at: 5 52 2140 4141 5
Size: 47 2088 2001 2081
Fragments arranged by size:
2088 2081 2001 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6217
StyI C'CwwG_G
Cuts at: 513 1065 2004 2020 3407 3656 513
Size: 552 939 16 1387 249 3074
Fragments arranged by size:
3074 1387 939 552 249 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2305 3416 4244 4261 4414 4599 5044
Size: 1255 725 1111 828 17 153 185 445
Cuts at: 5044 325
Size: 1498
Fragments arranged by size:
1498 1255 1111 828 725 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4463 6175 96
Size: 274 80 33 51 1443 2486 1712 138
Fragments arranged by size:
2486 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2791 2823 6177 6209 2791
Size: 32 3354 32 2799
Fragments arranged by size:
3354 2799 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 2619 4346 2619
Size: 1727 4490
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI