Restriction map for
pFN21AE5635
(Circular) (Six-base) MAPSORT of: pFN21AE5635.seq Check: 1991 from: 1 to: 6286
pFN21AE5635 6286 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2223 4096 278
Size: 53 83 186 936 687 1873 2468
Fragments arranged by size:
2468 1873 936 687 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2742 3629 1937
Size: 805 887 4594
Fragments arranged by size:
4594 887 805
Acc65I G'GTAC_C
Cuts at: 3607 3607
Size: 6286
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2098 2369 2552 2579 4349 5733 6001 1632
Size: 466 271 183 27 1770 1384 268 1917
Fragments arranged by size:
1917 1770 1384 466 271 268 183 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 2391 2701 2712 3177 3255 5185 6162 1203
Size: 1188 310 11 465 78 1930 977 1327
Fragments arranged by size:
1930 1327 1188 977 465 310 78 11
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 5015 5015
Size: 6286
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2328 1184
Size: 1144 5142
AlwNI CAG_nnn'CTG
Cuts at: 3028 4029 5601 3028
Size: 1001 1572 3713
Fragments arranged by size:
3713 1572 1001
ApaI G_GGCC'C
Cuts at: 2677 2677
Size: 6286
ApaLI G'TGCA_C
Cuts at: 4343 5499 4343
Size: 1156 5130
ApoI r'AATT_y
Cuts at: 1493 3107 3595 3821 3973 6082 1493
Size: 1614 488 226 152 2109 1697
Fragments arranged by size:
2109 1697 1614 488 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4840 160
Size: 872 3808 1606
Fragments arranged by size:
3808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 2670 2676 3390 3611 1870
Size: 73 727 6 714 221 4545
Fragments arranged by size:
4545 727 714 221 73 6
BamHI G'GATC_C
Cuts at: 3616 3983 3616
Size: 367 5919
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3607 5062 618
Size: 325 217 627 1820 1455 1842
Fragments arranged by size:
1842 1820 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2172 2481 2677 3605 1391
Size: 781 309 196 928 4072
Fragments arranged by size:
4072 928 781 309 196
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2205 928
Size: 971 306 5009
Fragments arranged by size:
5009 971 306
BbvCI CC'TCA_GC
Cuts at: 2645 2645
Size: 6286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4477 4511 1903
Size: 34 2540 34 3678
Fragments arranged by size:
3678 2540 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 2317 2938 3348 1548
Size: 769 621 410 4486
Fragments arranged by size:
4486 769 621 410
BfrBI ATG'CAT
Cuts at: 3271 3271
Size: 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4897 136
Size: 107 122 71 631 605 3225 1525
Fragments arranged by size:
3225 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2050 6281 2050
Size: 4231 2055
BlpI GC'TnA_GC
Cuts at: 2768 3703 2768
Size: 935 5351
Bme1580I G_kGCm'C
Cuts at: 1790 2677 3232 3358 4347 5503 1790
Size: 887 555 126 989 1156 2573
Fragments arranged by size:
2573 1156 989 887 555 126
BmgBI CAC'GTC
Cuts at: 1318 2185 2704 3397 1318
Size: 867 519 693 4207
Fragments arranged by size:
4207 867 693 519
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4017 4975 442
Size: 416 53 83 399 24 2600 958 1753
Fragments arranged by size:
2600 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2558 3031 3098 3332 3359 3534 4946 1239
Size: 1319 473 67 234 27 175 1412 2579
Fragments arranged by size:
2579 1412 1319 473 234 175 67 27
Bpu10I CC'TnA_GC
Cuts at: 2120 2645 2955 2120
Size: 525 310 5451
Fragments arranged by size:
5451 525 310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2642 2843 3122 4411 5276 5574 2642
Size: 201 279 1289 865 298 3354
Fragments arranged by size:
3354 1289 865 298 279 201
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2763 4949 882
Size: 617 1264 2186 2219
Fragments arranged by size:
2219 2186 1264 617
BsaAI yAC'GTr
Cuts at: 493 1204 2230 3256 4077 493
Size: 711 1026 1026 821 2702
Fragments arranged by size:
2702 1026 1026 821 711
BsaBI GATnn'nnATC
Cuts at: 2291 6280 2291
Size: 3989 2297
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2220 2454 4093 4475
Size: 53 83 186 936 687 234 1639 382
Cuts at: 4475 5110 275
Size: 635 1451
Fragments arranged by size:
1639 1451 936 687 635 382 234 186
83 53
BsaWI w'CCGG_w
Cuts at: 4719 5117 5391 5538 5949 4719
Size: 398 274 147 411 5056
Fragments arranged by size:
5056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6061
Fragments arranged by size:
6061 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2414 2832 1186
Size: 840 388 418 4640
Fragments arranged by size:
4640 840 418 388
BseYI C'CCAG_C
Cuts at: 2024 2243 2482 2969 5489 5985 2024
Size: 219 239 487 2520 496 2325
Fragments arranged by size:
2520 2325 496 487 239 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2044 2390 2671 1163
Size: 573 308 346 281 4778
Fragments arranged by size:
4778 573 346 308 281
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2106 3736 4497 5525 1540
Size: 275 186 105 1630 761 1028 2301
Fragments arranged by size:
2301 1630 1028 761 275 186 105
BsiHKAI G_wGCw'C
Cuts at: 2769 3492 3578 4347 4432 5503 2769
Size: 723 86 769 85 1071 3552
Fragments arranged by size:
3552 1071 769 723 86 85
BsmI GAATG_Cn'
Cuts at: 2215 3797 3890 2215
Size: 1582 93 4611
Fragments arranged by size:
4611 1582 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2481 2677 2769 3232 3358 3492
Size: 399 382 309 196 92 463 126 134
Cuts at: 3492 3578 3605 4347 4432 5503 1391
Size: 86 27 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 463 399 382 309 196
134 126 92 86 85 27
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 4175 5905 4175
Size: 1730 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3174 3641 844
Size: 2330 467 3489
Fragments arranged by size:
3489 2330 467
BsrBI CCG'CTC
Cuts at: 1394 1823 2469 4173 1394
Size: 429 646 1704 3507
Fragments arranged by size:
3507 1704 646 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2310 3043 4781 4955 65
Size: 1183 1062 733 1738 174 1396
Fragments arranged by size:
1738 1396 1183 1062 733 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3018 4930 5117 1700
Size: 110 1208 1912 187 2869
Fragments arranged by size:
2869 1912 1208 187 110
BsrGI T'GTAC_A
Cuts at: 96 3207 96
Size: 3111 3175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 4346 5358 4346
Size: 1012 5274
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3028 794
Size: 2234 4052
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6286
BstXI CCAn_nnnn'nTGG
Cuts at: 2977 2977
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 2050 2251 2403 3378 3501 3616 3983 4368
Size: 78 201 152 975 123 115 367 385
Cuts at: 4368 4385 5837 5923 5935 6281 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 975 385 367 346 201 152
123 115 86 78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2163 3297 513
Size: 552 1098 1134 3502
Fragments arranged by size:
3502 1134 1098 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5547
Fragments arranged by size:
5547 683 56
BtsI GCAGTG_nn'
Cuts at: 2320 3802 4596 4616 2320
Size: 1482 794 20 3990
Fragments arranged by size:
3990 1482 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2268 2602
Size: 54 1425 50 275 18 273 165 334
Cuts at: 2602 2886 2979 3303 3733 4622 6251 8
Size: 284 93 324 430 889 1629 43
Fragments arranged by size:
1629 1425 889 430 334 324 284 275
273 165 93 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2103 3733 1537
Size: 275 291 1630 4090
Fragments arranged by size:
4090 1630 291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4216 1353
Size: 2863 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4873 5247 5393 387
Size: 1064 138 3284 374 146 1280
Fragments arranged by size:
3284 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2369 2552 2558 2579 3031 3098
Size: 393 466 271 183 6 21 452 67
Cuts at: 3098 3332 3359 3534 4349 4946 5733 6001 1239
Size: 234 27 175 815 597 787 268 1524
Fragments arranged by size:
1524 815 787 597 466 452 393 271
268 234 183 175 67 27 21 6
EcoO109I rG'GnC_Cy
Cuts at: 2344 2641 3201 2344
Size: 297 560 5429
Fragments arranged by size:
5429 560 297
EcoRI G'AATT_C
Cuts at: 3595 3973 3595
Size: 378 5908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 6286
FspI TGC'GCA
Cuts at: 2291 4792 2291
Size: 2501 3785
FspAI rTGC'GCAy
Cuts at: 2291 2291
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 2127 2528 2697 3457 5433 1678
Size: 449 401 169 760 1976 2531
Fragments arranged by size:
2531 1976 760 449 401 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2948 2980 3084 3116 4927
Size: 32 135 32 1376 32 104 32 1811
Cuts at: 4927 4959 5001 5033 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1811 1376 135 104 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2224 3630 3876 669
Size: 988 281 286 1406 246 3079
Fragments arranged by size:
3079 1406 988 286 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6286
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 3611 3611
Size: 6286
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5400 5584 1305
Size: 4095 184 2007
Fragments arranged by size:
4095 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2981 10
Size: 54 1425 343 1149 3315
Fragments arranged by size:
3315 1425 1149 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2711 3581 4244 4603 4762 518
Size: 648 1545 870 663 359 159 2042
Fragments arranged by size:
2042 1545 870 663 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2280 3485 3702 4379 5527 5772 1606
Size: 674 1205 217 677 1148 245 2120
Fragments arranged by size:
2120 1205 1148 677 674 245 217
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5734
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NsiI A_TGCA'T
Cuts at: 3273 3273
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2395 2716 3181 3271 3644 5189 1132
Size: 97 1166 321 465 90 373 1545 2229
Fragments arranged by size:
2229 1545 1166 465 373 321 97 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 2391 2712 3177 5185 2391
Size: 321 465 2008 3492
Fragments arranged by size:
3492 2008 465 321
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2629 1108
Size: 1521 4765
PfoI T'CCnGG_A
Cuts at: 3171 3336 3566 3171
Size: 165 230 5891
Fragments arranged by size:
5891 230 165
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 5868 5900 4355
Size: 32 1481 32 4741
Fragments arranged by size:
4741 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2641 3201 2641
Size: 560 5726
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6286
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PspOMI G'GGCC_C
Cuts at: 2673 2673
Size: 6286
PstI C_TGCA'G
Cuts at: 830 2307 2442 3033 3638 830
Size: 1477 135 591 605 3478
Fragments arranged by size:
3478 1477 605 591 135
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
PvuII CAG'CTG
Cuts at: 3485 3485
Size: 6286
SalI G'TCGA_C
Cuts at: 1936 3628 1936
Size: 1692 4594
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 4534 6246 4534
Size: 1712 4574
SfcI C'TryA_G
Cuts at: 826 1045 2275 2303 2438 3029 3634 4769 5450
Size: 219 1230 28 135 591 605 1135 681
Cuts at: 5450 5641 826
Size: 191 1471
Fragments arranged by size:
1471 1230 1135 681 605 591 219 191
135 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 2672 2678 3392 3613 1872
Size: 800 6 714 221 4545
Fragments arranged by size:
4545 800 714 221 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2657 2858 3137 4390 5291 5553
Size: 197 926 714 201 279 1253 901 262
Cuts at: 5553 820
Size: 1553
Fragments arranged by size:
1553 1253 926 901 714 279 262 201
197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 3644 1132
Size: 2512 3774
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6286
StyI C'CwwG_G
Cuts at: 513 1065 3003 3462 3725 513
Size: 552 1938 459 263 3074
Fragments arranged by size:
3074 1938 552 459 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2491 3227 4313 4330 4483 4668 5113
Size: 1255 911 736 1086 17 153 185 445
Cuts at: 5113 325
Size: 1498
Fragments arranged by size:
1498 1255 1086 911 736 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2055 2898 3207
Size: 274 80 33 51 1443 78 843 309
Cuts at: 3207 4532 6244 96
Size: 1325 1712 138
Fragments arranged by size:
1712 1443 1325 843 309 274 138 80
78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6246 6278 6246
Size: 32 6254
Tth111I GACn'n_nGTC
Cuts at: 1316 2087 1316
Size: 771 5515
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2251 2335 2251
Size: 84 6202
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 2670 2676 3390 3611 1870
Size: 800 6 714 221 4545
Fragments arranged by size:
4545 800 714 221 6
XmnI GAAnn'nnTTC
Cuts at: 4415 4415
Size: 6286
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2221 4094 276
Size: 53 83 186 936 687 1873 2468
Fragments arranged by size:
2468 1873 936 687 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI