Restriction map for
pFN21AE5633
(Circular) (Six-base) MAPSORT of: pFN21AE5633.seq Check: 6423 from: 1 to: 6181
pFN21AE5633 6181 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2177 3370 2177
Size: 1193 4988
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3991 278
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3524 1937
Size: 1587 4594
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 2576 4308 4681 1563
Size: 1013 1732 373 3063
Fragments arranged by size:
3063 1732 1013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 2576 4244 5628 5896 1632
Size: 707 237 1668 1384 268 1917
Fragments arranged by size:
1917 1668 1384 707 268 237
AfeI AGC'GCT
Cuts at: 3449 3449
Size: 6181
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 5080 6057 1203
Size: 3877 977 1327
Fragments arranged by size:
3877 1327 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 4910 4910
Size: 6181
AleI CACnn'nnGTG
Cuts at: 1166 2963 1166
Size: 1797 4384
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6181
AlwNI CAG_nnn'CTG
Cuts at: 2306 3924 5496 2306
Size: 1618 1572 2991
Fragments arranged by size:
2991 1618 1572
ApaLI G'TGCA_C
Cuts at: 4238 5394 4238
Size: 1156 5025
ApoI r'AATT_y
Cuts at: 1493 2890 3490 3716 3868 5977 1493
Size: 1397 600 226 152 2109 1697
Fragments arranged by size:
2109 1697 1397 600 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4735 160
Size: 872 3703 1606
Fragments arranged by size:
3703 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 2949 3506 1870
Size: 73 1006 557 4545
Fragments arranged by size:
4545 1006 557 73
BamHI G'GATC_C
Cuts at: 3511 3878 3511
Size: 367 5814
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2165 2513 2729 2834
Size: 325 217 627 357 21 348 216 105
Cuts at: 2834 3502 4957 618
Size: 668 1455 1842
Fragments arranged by size:
1842 1455 668 627 357 348 325 217
216 105 21
BanII G_rGCy'C
Cuts at: 1391 3282 3500 1391
Size: 1891 218 4072
Fragments arranged by size:
4072 1891 218
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3103 928
Size: 971 1204 4006
Fragments arranged by size:
4006 1204 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4372 4406 1903
Size: 34 2435 34 3678
Fragments arranged by size:
3678 2435 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4073 5283 3410
Size: 663 1210 4308
Fragments arranged by size:
4308 1210 663
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6181
BfrBI ATG'CAT
Cuts at: 3242 3242
Size: 6181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2084 2163 4792
Size: 107 122 71 631 605 412 79 2629
Cuts at: 4792 136
Size: 1525
Fragments arranged by size:
2629 1525 631 605 412 122 107 79
71
BglII A'GATC_T
Cuts at: 3076 6176 3076
Size: 3100 3081
BlpI GC'TnA_GC
Cuts at: 3598 3598
Size: 6181
Bme1580I G_kGCm'C
Cuts at: 1790 2755 2837 2970 4242 5398 1790
Size: 965 82 133 1272 1156 2573
Fragments arranged by size:
2573 1272 1156 965 133 82
BmgBI CAC'GTC
Cuts at: 1318 2992 1318
Size: 1674 4507
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2231 2344 3241
Size: 416 53 83 399 24 814 113 897
Cuts at: 3241 3912 4870 442
Size: 671 958 1753
Fragments arranged by size:
1753 958 897 814 671 416 399 113
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2308 2499 2627 3143 3236 4841 1239
Size: 1069 191 128 516 93 1605 2579
Fragments arranged by size:
2579 1605 1069 516 191 128 93
Bpu10I CC'TnA_GC
Cuts at: 3009 3451 3009
Size: 442 5739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3321 4306 5171 5469 3321
Size: 985 865 298 4033
Fragments arranged by size:
4033 985 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4844 882
Size: 617 3345 2219
Fragments arranged by size:
3345 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2125 2320 3972 493
Size: 711 921 195 1652 2702
Fragments arranged by size:
2702 1652 921 711 195
BsaBI GATnn'nnATC
Cuts at: 6175 6175
Size: 6181
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3988 4370 5005 275
Size: 53 83 186 936 2455 382 635 1451
Fragments arranged by size:
2455 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4614 5012 5286 5433 5844 4614
Size: 398 274 147 411 4951
Fragments arranged by size:
4951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5956
Fragments arranged by size:
5956 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3164 3167 3434 1186
Size: 1978 3 267 3933
Fragments arranged by size:
3933 1978 267 3
BseYI C'CCAG_C
Cuts at: 2091 2095 2256 2268 2903 3295 5384 5880 2091
Size: 4 161 12 635 392 2089 496 2392
Fragments arranged by size:
2392 2089 635 496 392 161 12 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3071 1163
Size: 573 1335 4273
Fragments arranged by size:
4273 1335 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2489 3108 3631 4392 5420 1540
Size: 275 186 488 619 523 761 1028 2301
Fragments arranged by size:
2301 1028 761 619 523 488 275 186
BsiHKAI G_wGCw'C
Cuts at: 2409 2715 4242 4327 5398 2409
Size: 306 1527 85 1071 3192
Fragments arranged by size:
3192 1527 1071 306 85
BsmI GAATG_Cn'
Cuts at: 3692 3785 3692
Size: 93 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2409 2715 2755 2837 2970 3282 3500
Size: 399 619 306 40 82 133 312 218
Cuts at: 3500 4242 4327 5398 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 619 399 312 306 218
133 85 82 40
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 2652 4070 5800 2652
Size: 1418 1730 3033
Fragments arranged by size:
3033 1730 1418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2009 2177 2782 3370 3536 844
Size: 1165 168 605 588 166 3489
Fragments arranged by size:
3489 1165 605 588 168 166
BsrBI CCG'CTC
Cuts at: 1394 1823 4068 1394
Size: 429 2245 3507
Fragments arranged by size:
3507 2245 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2469 4676 4850 65
Size: 1183 1221 2207 174 1396
Fragments arranged by size:
2207 1396 1221 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2816 4825 5012 1700
Size: 110 1006 2009 187 2869
Fragments arranged by size:
2869 2009 1006 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6181
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6181
BssSI C'ACGA_G
Cuts at: 4241 5253 4241
Size: 1012 5169
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3385 794
Size: 2591 3590
BstBI TT'CG_AA
Cuts at: 4985 4985
Size: 6181
BstEII G'GTnAC_C
Cuts at: 1184 2794 1184
Size: 1610 4571
BstXI CCAn_nnnn'nTGG
Cuts at: 2599 2599
Size: 6181
BstYI r'GATC_y
Cuts at: 1972 2057 2934 3076 3511 3878 4263 4280 5732
Size: 85 877 142 435 367 385 17 1452
Cuts at: 5732 5818 5830 6176 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 877 435 385 367 346 142
86 85 17 12
Bsu36I CC'TnA_GG
Cuts at: 2741 3272 2741
Size: 531 5650
BtgI C'CryG_G
Cuts at: 513 1065 2004 2112 2674 2700 3169 3393 3465
Size: 552 939 108 562 26 469 224 72
Cuts at: 3465 513
Size: 3229
Fragments arranged by size:
3229 939 562 552 469 224 108 72
26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5442
Fragments arranged by size:
5442 683 56
BtsI GCAGTG_nn'
Cuts at: 3165 3697 4491 4511 3165
Size: 532 794 20 4835
Fragments arranged by size:
4835 794 532 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6146
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DraIII CAC_nnn'GTG
Cuts at: 2151 2151
Size: 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2366 5188 809
Size: 507 1050 2822 1802
Fragments arranged by size:
2822 1802 1050 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2486 2782
Size: 54 1425 50 275 18 279 377 296
Cuts at: 2782 3033 3628 4517 6146 8
Size: 251 595 889 1629 43
Fragments arranged by size:
1629 1425 889 595 377 296 279 275
251 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2486 3628 1537
Size: 275 674 1142 4090
Fragments arranged by size:
4090 1142 674 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3065 4111 1353
Size: 1712 1046 3423
Fragments arranged by size:
3423 1712 1046
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4768 5142 5288 387
Size: 1064 138 3179 374 146 1280
Fragments arranged by size:
3179 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2308 2339 2499 2576 2627 3143 3236
Size: 393 676 31 160 77 51 516 93
Cuts at: 3236 4244 4841 5628 5896 1239
Size: 1008 597 787 268 1524
Fragments arranged by size:
1524 1008 787 676 597 516 393 268
160 93 77 51 31
EcoO109I rG'GnC_Cy
Cuts at: 2155 3069 2155
Size: 914 5267
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
FspI TGC'GCA
Cuts at: 2660 3378 3437 4687 2660
Size: 718 59 1250 4154
Fragments arranged by size:
4154 1250 718 59
HaeII r_GCGC'y
Cuts at: 1678 3451 5328 1678
Size: 1773 1877 2531
Fragments arranged by size:
2531 1877 1773
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2217 2249 4822 4854 4896
Size: 32 135 32 645 32 2573 32 42
Cuts at: 4896 4928 1373
Size: 32 2626
Fragments arranged by size:
2626 2573 645 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 2401 3525 3771 669
Size: 988 281 238 225 1124 246 3079
Fragments arranged by size:
3079 1124 988 281 246 238 225
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6181
HpaI GTT'AAC
Cuts at: 3771 3771
Size: 6181
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5295 5479 1305
Size: 3990 184 2007
Fragments arranged by size:
3990 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2784 10
Size: 54 1425 343 952 3407
Fragments arranged by size:
3407 1425 952 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2651 2654 2963 3247 4139 4498 4657
Size: 648 1485 3 309 284 892 359 159
Cuts at: 4657 518
Size: 2042
Fragments arranged by size:
2042 1485 892 648 359 309 284 159
3
MspA1I CmG'CkG
Cuts at: 1606 2026 2095 2676 2788 3055 3289 3597 4274
Size: 420 69 581 112 267 234 308 677
Cuts at: 4274 5422 5667 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 581 420 308 267 245
234 112 69
NcoI C'CATG_G
Cuts at: 513 1065 2004 2700 513
Size: 552 939 696 3994
Fragments arranged by size:
3994 939 696 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6181
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6181
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NsiI A_TGCA'T
Cuts at: 3244 3244
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 3185 3414 3539 5084 1132
Size: 97 1956 229 125 1545 2229
Fragments arranged by size:
2229 1956 1545 229 125 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6181
PciI A'CATG_T
Cuts at: 5080 5080
Size: 6181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6181
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PmlI CAC'GTG
Cuts at: 2320 2320
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4250 4282 5763 5795 4250
Size: 32 1481 32 4636
Fragments arranged by size:
4636 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3069 3069
Size: 6181
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3207 3751 3207
Size: 544 5637
PstI C_TGCA'G
Cuts at: 830 2793 3533 830
Size: 1963 740 3478
Fragments arranged by size:
3478 1963 740
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
PvuII CAG'CTG
Cuts at: 2095 2788 3289 2095
Size: 693 501 4987
Fragments arranged by size:
4987 693 501
RsrII CG'GwC_CG
Cuts at: 3390 3390
Size: 6181
SacII CC_GC'GG
Cuts at: 2677 2677
Size: 6181
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SapI GCTCTTCn'nnn_
Cuts at: 3065 3065
Size: 6181
SbfI CC_TGCA'GG
Cuts at: 3533 3533
Size: 6181
ScaI AGT'ACT
Cuts at: 2375 4429 6141 2375
Size: 2054 1712 2415
Fragments arranged by size:
2415 2054 1712
SfcI C'TryA_G
Cuts at: 826 1045 2789 3529 4664 5345 5536 826
Size: 219 1744 740 1135 681 191 1471
Fragments arranged by size:
1744 1471 1135 740 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 2951 3508 1872
Size: 1079 557 4545
Fragments arranged by size:
4545 1079 557
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3336 4285 5186 5448 820
Size: 197 926 1393 949 901 262 1553
Fragments arranged by size:
1553 1393 949 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3972 493
Size: 3479 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 3539 1132
Size: 2407 3774
SspI AAT'ATT
Cuts at: 5 52 4105 5
Size: 47 4053 2081
Fragments arranged by size:
4053 2081 47
StuI AGG'CCT
Cuts at: 1517 2229 3456 1517
Size: 712 1227 4242
Fragments arranged by size:
4242 1227 712
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 2140 2517 2700 3310 3620
Size: 552 939 75 61 377 183 610 310
Cuts at: 3620 513
Size: 3074
Fragments arranged by size:
3074 939 610 552 377 310 183 75
61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3034 4208 4225 4378 4563 5008 325
Size: 1255 1454 1174 17 153 185 445 1498
Fragments arranged by size:
1498 1454 1255 1174 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2373 4427 6139
Size: 274 80 33 51 1443 396 2054 1712
Cuts at: 6139 96
Size: 138
Fragments arranged by size:
2054 1712 1443 396 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2091 2123 2309 2341 3346 3378 6141 6173 2091
Size: 32 186 32 1005 32 2763 32 2099
Fragments arranged by size:
2763 2099 1005 186 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2846 1316
Size: 1530 4651
XbaI T'CTAG_A
Cuts at: 3517 3517
Size: 6181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2989 3028 2989
Size: 39 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6181
XmaI C'CCGG_G
Cuts at: 1870 2949 3506 1870
Size: 1079 557 4545
Fragments arranged by size:
4545 1079 557
XmnI GAAnn'nnTTC
Cuts at: 2307 4310 2307
Size: 2003 4178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3989 276
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PspOMI PspXI PsrI SacI SanDI SexAI SfiI SfoI
SrfI SwaI