Restriction map for
pFN21AE5632
(Circular) (Six-base) MAPSORT of: pFN21AE5632.seq Check: 4596 from: 1 to: 6175
pFN21AE5632 6175 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3985 278
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3470 3518 1937
Size: 1533 48 4594
Fragments arranged by size:
4594 1533 48
Acc65I G'GTAC_C
Cuts at: 3109 3496 3109
Size: 387 5788
AclI AA'CG_TT
Cuts at: 1563 4302 4675 1563
Size: 2739 373 3063
Fragments arranged by size:
3063 2739 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4238 5622 5890 1632
Size: 2606 1384 268 1917
Fragments arranged by size:
2606 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5978
AflIII A'CryG_T
Cuts at: 1203 5074 6051 1203
Size: 3871 977 1327
Fragments arranged by size:
3871 1327 977
AgeI A'CCGG_T
Cuts at: 5006 5006
Size: 6175
AhdI GACnn_n'nnGTC
Cuts at: 4904 4904
Size: 6175
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6175
AlwNI CAG_nnn'CTG
Cuts at: 2549 3242 3266 3918 5490 2549
Size: 693 24 652 1572 3234
Fragments arranged by size:
3234 1572 693 652 24
ApaLI G'TGCA_C
Cuts at: 4232 5388 4232
Size: 1156 5019
ApoI r'AATT_y
Cuts at: 1493 2220 2569 2887 3484 3710 3862 5971 1493
Size: 727 349 318 597 226 152 2109 1697
Fragments arranged by size:
2109 1697 727 597 349 318 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4729 160
Size: 872 3697 1606
Fragments arranged by size:
3697 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 3500 1870
Size: 73 1557 4545
Fragments arranged by size:
4545 1557 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2976 3009 2976
Size: 33 6142
BamHI G'GATC_C
Cuts at: 3505 3872 3505
Size: 367 5808
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2968 3109 3496 4951 618
Size: 325 217 627 1181 141 387 1455 1842
Fragments arranged by size:
1842 1455 1181 627 387 325 217 141
BanII G_rGCy'C
Cuts at: 1391 3494 1391
Size: 2103 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 2942 3096 3131 928
Size: 971 126 917 154 35 3972
Fragments arranged by size:
3972 971 917 154 126 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4366 4400 1903
Size: 34 2429 34 3678
Fragments arranged by size:
3678 2429 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4067 5277 4067
Size: 1210 4965
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4786 136
Size: 107 122 71 631 605 3114 1525
Fragments arranged by size:
3114 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2834 6170 2834
Size: 3336 2839
BlpI GC'TnA_GC
Cuts at: 3592 3592
Size: 6175
Bme1580I G_kGCm'C
Cuts at: 1790 4236 5392 1790
Size: 2446 1156 2573
Fragments arranged by size:
2573 2446 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3460 3906 4864
Size: 416 53 83 399 24 2043 446 958
Cuts at: 4864 442
Size: 1753
Fragments arranged by size:
2043 1753 958 446 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3381 3381
Size: 6175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 3114 3165 4835 1239
Size: 813 1062 51 1670 2579
Fragments arranged by size:
2579 1670 1062 813 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4300 5165 5463 4300
Size: 865 298 5012
Fragments arranged by size:
5012 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3239 3263 4838 882
Size: 617 1740 24 1575 2219
Fragments arranged by size:
2219 1740 1575 617 24
BsaAI yAC'GTr
Cuts at: 493 1204 2648 3966 493
Size: 711 1444 1318 2702
Fragments arranged by size:
2702 1444 1318 711
BsaBI GATnn'nnATC
Cuts at: 6169 6169
Size: 6175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3982 4364 4999 275
Size: 53 83 186 936 2449 382 635 1451
Fragments arranged by size:
2449 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4608 5006 5280 5427 5838 4608
Size: 398 274 147 411 4945
Fragments arranged by size:
4945 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2050 2080 1178
Size: 30 165 30 647 30 5273
Fragments arranged by size:
5273 647 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6175
BseYI C'CCAG_C
Cuts at: 3200 3340 5378 5874 3200
Size: 140 2038 496 3501
Fragments arranged by size:
3501 2038 496 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2766 1163
Size: 573 1030 4572
Fragments arranged by size:
4572 1030 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3474 3625 4386 5414 1540
Size: 275 186 1473 151 761 1028 2301
Fragments arranged by size:
2301 1473 1028 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 2679 4236 4321 5392 2679
Size: 1557 85 1071 3462
Fragments arranged by size:
3462 1557 1071 85
BsmI GAATG_Cn'
Cuts at: 2670 3686 3779 2670
Size: 1016 93 5066
Fragments arranged by size:
5066 1016 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2679 3494 4236 4321 5392 1391
Size: 399 889 815 742 85 1071 2174
Fragments arranged by size:
2174 1071 889 815 742 399 85
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 4064 5794 4064
Size: 1730 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3530 844
Size: 2686 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4062 1394
Size: 429 2239 3507
Fragments arranged by size:
3507 2239 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2140 4670 4844 65
Size: 1183 892 2530 174 1396
Fragments arranged by size:
2530 1396 1183 892 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4819 5006 1700
Size: 110 3009 187 2869
Fragments arranged by size:
3009 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6175
BssSI C'ACGA_G
Cuts at: 3210 4235 5247 3210
Size: 1025 1012 4138
Fragments arranged by size:
4138 1025 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6175
BstBI TT'CG_AA
Cuts at: 4979 4979
Size: 6175
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6175
BstXI CCAn_nnnn'nTGG
Cuts at: 2736 2736
Size: 6175
BstYI r'GATC_y
Cuts at: 1972 2834 3505 3872 4257 4274 5726 5812 5824
Size: 862 671 367 385 17 1452 86 12
Cuts at: 5824 6170 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 862 671 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4684
Fragments arranged by size:
4684 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5436
Fragments arranged by size:
5436 683 56
BtsI GCAGTG_nn'
Cuts at: 2127 2644 3324 3691 4485 4505 2127
Size: 517 680 367 794 20 3797
Fragments arranged by size:
3797 794 680 517 367 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6140
DraI TTT'AAA
Cuts at: 2358 3479 2358
Size: 1121 5054
DraIII CAC_nnn'GTG
Cuts at: 3212 3212
Size: 6175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5182 809
Size: 507 3866 1802
Fragments arranged by size:
3866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3622 4511 6140
Size: 54 1425 50 275 18 1792 889 1629
Cuts at: 6140 8
Size: 43
Fragments arranged by size:
1792 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3622 1537
Size: 275 1810 4090
Fragments arranged by size:
4090 1810 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2872 2912 3306 4105 1353
Size: 1519 40 394 799 3423
Fragments arranged by size:
3423 1519 799 394 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2432 4762 5136 5282 387
Size: 1064 138 843 2330 374 146 1280
Fragments arranged by size:
2330 1280 1064 843 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 3114 3165 4238 4835 5622 5890
Size: 393 420 1062 51 1073 597 787 268
Cuts at: 5890 1239
Size: 1524
Fragments arranged by size:
1524 1073 1062 787 597 420 393 268
51
EcoRI G'AATT_C
Cuts at: 3484 3862 3484
Size: 378 5797
EcoRV GAT'ATC
Cuts at: 2287 2287
Size: 6175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2649 3246 2649
Size: 597 5578
FspI TGC'GCA
Cuts at: 4681 4681
Size: 6175
HaeII r_GCGC'y
Cuts at: 1678 5322 1678
Size: 3644 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2242 2274 3181
Size: 32 135 32 431 32 207 32 907
Cuts at: 3181 3213 4816 4848 4890 4922 1373
Size: 32 1603 32 42 32 2626
Fragments arranged by size:
2626 1603 907 431 207 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3471 3519 3765 669
Size: 988 281 1533 48 246 3079
Fragments arranged by size:
3079 1533 988 281 246 48
HindIII A'AGCT_T
Cuts at: 748 2351 3420 748
Size: 1603 1069 3503
Fragments arranged by size:
3503 1603 1069
HpaI GTT'AAC
Cuts at: 3765 3765
Size: 6175
KpnI G_GTAC'C
Cuts at: 3113 3500 3113
Size: 387 5788
MfeI C'AATT_G
Cuts at: 2812 3774 2812
Size: 962 5213
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2641 5289 5473 1305
Size: 1336 2648 184 2007
Fragments arranged by size:
2648 2007 1336 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4353
Fragments arranged by size:
4353 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4133 4492 4651 518
Size: 648 2967 359 159 2042
Fragments arranged by size:
2967 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2072 2095 3106 3591 4268 5416 5661 1606
Size: 466 23 1011 485 677 1148 245 2120
Fragments arranged by size:
2120 1148 1011 677 485 466 245 23
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4684
Fragments arranged by size:
4684 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6175
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6175
NotI GC'GGCC_GC
Cuts at: 3622 3622
Size: 6175
NspI r_CATG'y
Cuts at: 1132 1229 3164 3533 5078 1132
Size: 97 1935 369 1545 2229
Fragments arranged by size:
2229 1935 1545 369 97
PasI CC'CwG_GG
Cuts at: 1348 3275 1348
Size: 1927 4248
PciI A'CATG_T
Cuts at: 5074 5074
Size: 6175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3032 3441 1108
Size: 1924 409 3842
Fragments arranged by size:
3842 1924 409
PfoI T'CCnGG_A
Cuts at: 2150 2150
Size: 6175
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 5757 5789 4244
Size: 32 1481 32 4630
Fragments arranged by size:
4630 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6175
PsiI TTA'TAA
Cuts at: 3745 3745
Size: 6175
PstI C_TGCA'G
Cuts at: 830 2041 3262 3527 830
Size: 1211 1221 265 3478
Fragments arranged by size:
3478 1221 1211 265
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
PvuII CAG'CTG
Cuts at: 2095 3106 2095
Size: 1011 5164
SalI G'TCGA_C
Cuts at: 1936 3469 3517 1936
Size: 1533 48 4594
Fragments arranged by size:
4594 1533 48
SbfI CC_TGCA'GG
Cuts at: 3527 3527
Size: 6175
ScaI AGT'ACT
Cuts at: 4423 6135 4423
Size: 1712 4463
SfcI C'TryA_G
Cuts at: 826 1045 2037 2988 3258 3523 4658 5339 5530
Size: 219 992 951 270 265 1135 681 191
Cuts at: 5530 826
Size: 1471
Fragments arranged by size:
1471 1135 992 951 681 270 265 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6175
SmaI CCC'GGG
Cuts at: 1872 3502 1872
Size: 1630 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4279 5180 5442 820
Size: 197 926 2336 901 262 1553
Fragments arranged by size:
2336 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2648 3966 493
Size: 2155 1318 2702
Fragments arranged by size:
2702 2155 1318
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 3533 1132
Size: 2401 3774
SspI AAT'ATT
Cuts at: 5 52 4099 5
Size: 47 4047 2081
Fragments arranged by size:
4047 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6175
StyI C'CwwG_G
Cuts at: 513 1065 2004 3614 513
Size: 552 939 1610 3074
Fragments arranged by size:
3074 1610 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4202 4219 4372 4557 5002 325
Size: 1255 2622 17 153 185 445 1498
Fragments arranged by size:
2622 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4421 6133 96
Size: 274 80 33 51 1443 2444 1712 138
Fragments arranged by size:
2444 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2050 2082 6135 6167 2050
Size: 32 4053 32 2058
Fragments arranged by size:
4053 2058 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6175
XbaI T'CTAG_A
Cuts at: 3511 3511
Size: 6175
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6175
XmaI C'CCGG_G
Cuts at: 1870 3500 1870
Size: 1630 4545
XmnI GAAnn'nnTTC
Cuts at: 3421 4304 3421
Size: 883 5292
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3983 276
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI