Restriction map for
pFN21AE5630
(Circular) (Six-base) MAPSORT of: pFN21AE5630.seq Check: 5432 from: 1 to: 6247
pFN21AE5630 6247 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3222 3222
Size: 6247
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4057 278
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3590 1937
Size: 1653 4594
Acc65I G'GTAC_C
Cuts at: 2652 3568 2652
Size: 916 5331
AclI AA'CG_TT
Cuts at: 1563 4374 4747 1563
Size: 2811 373 3063
Fragments arranged by size:
3063 2811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2560 2642 2769 3401 4310 5694 5962
Size: 578 350 82 127 632 909 1384 268
Cuts at: 5962 1632
Size: 1917
Fragments arranged by size:
1917 1384 909 632 578 350 268 127
82
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 5146 6123 1203
Size: 3943 977 1327
Fragments arranged by size:
3943 1327 977
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 4976 4976
Size: 6247
AleI CACnn'nnGTG
Cuts at: 1166 2462 2789 1166
Size: 1296 327 4624
Fragments arranged by size:
4624 1296 327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6247
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 6215
AlwNI CAG_nnn'CTG
Cuts at: 2023 2071 2356 2992 3187 3430 3990 5562 2023
Size: 48 285 636 195 243 560 1572 2708
Fragments arranged by size:
2708 1572 636 560 285 243 195 48
ApaLI G'TGCA_C
Cuts at: 4304 5460 4304
Size: 1156 5091
ApoI r'AATT_y
Cuts at: 1493 2560 2593 2761 3434 3556 3782 3934 6043
Size: 1067 33 168 673 122 226 152 2109
Cuts at: 6043 1493
Size: 1697
Fragments arranged by size:
2109 1697 1067 673 226 168 152 122
33
AseI AT'TA_AT
Cuts at: 160 1032 4801 160
Size: 872 3769 1606
Fragments arranged by size:
3769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 2366 2372 3572 1870
Size: 73 423 6 1200 4545
Fragments arranged by size:
4545 1200 423 73 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2636 2669 2636
Size: 33 6214
BamHI G'GATC_C
Cuts at: 2917 3577 3944 2917
Size: 660 367 5220
Fragments arranged by size:
5220 660 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2652 3568 5023 618
Size: 325 217 627 865 916 1455 1842
Fragments arranged by size:
1842 1455 916 865 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2210 2373 2448 2482 3129 3566 1391
Size: 819 163 75 34 647 437 4072
Fragments arranged by size:
4072 819 647 437 163 75 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2407 2760 3304 3402 928
Size: 971 508 353 544 98 3773
Fragments arranged by size:
3773 971 544 508 353 98
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4438 4472 1903
Size: 34 2501 34 3678
Fragments arranged by size:
3678 2501 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4139 5349 4139
Size: 1210 5037
BclI T'GATC_A
Cuts at: 1548 2535 1548
Size: 987 5260
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4858 136
Size: 107 122 71 631 605 3186 1525
Fragments arranged by size:
3186 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6242 6242
Size: 6247
BlpI GC'TnA_GC
Cuts at: 3664 3664
Size: 6247
Bme1580I G_kGCm'C
Cuts at: 1790 3058 4308 5464 1790
Size: 1268 1250 1156 2573
Fragments arranged by size:
2573 1268 1250 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6247
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2980 3978 4936
Size: 416 53 83 399 24 1563 998 958
Cuts at: 4936 442
Size: 1753
Fragments arranged by size:
1753 1563 998 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2026 2828 3019 3305 4907 1239
Size: 787 802 191 286 1602 2579
Fragments arranged by size:
2579 1602 802 787 286 191
Bpu10I CC'TnA_GC
Cuts at: 3241 3241
Size: 6247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4372 5237 5535 4372
Size: 865 298 5084
Fragments arranged by size:
5084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3281 4910 882
Size: 617 1782 1629 2219
Fragments arranged by size:
2219 1782 1629 617
BsaAI yAC'GTr
Cuts at: 493 1204 4038 493
Size: 711 2834 2702
Fragments arranged by size:
2834 2702 711
BsaBI GATnn'nnATC
Cuts at: 6241 6241
Size: 6247
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4054 4436 5071 275
Size: 53 83 186 936 2521 382 635 1451
Fragments arranged by size:
2521 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3153 4680 5078 5352 5499 5910 3153
Size: 1527 398 274 147 411 3490
Fragments arranged by size:
3490 1527 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3017 3047 1178
Size: 30 165 30 1614 30 4378
Fragments arranged by size:
4378 1614 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 1186
Size: 955 5292
BseYI C'CCAG_C
Cuts at: 2505 5450 5946 2505
Size: 2945 496 2806
Fragments arranged by size:
2945 2806 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5674
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2925 3697 4458 5486 1540
Size: 275 186 924 772 761 1028 2301
Fragments arranged by size:
2301 1028 924 772 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2240 3239 4308 4393 5464 2240
Size: 999 1069 85 1071 3023
Fragments arranged by size:
3023 1071 1069 999 85
BsmI GAATG_Cn'
Cuts at: 3758 3851 3758
Size: 93 6154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2240 2373 2448 2482 3058 3129
Size: 399 420 30 133 75 34 576 71
Cuts at: 3129 3239 3566 4308 4393 5464 1391
Size: 110 327 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 576 420 399 327 133
110 85 75 71 34 30
BspEI T'CCGG_A
Cuts at: 3153 5910 3153
Size: 2757 3490
BspHI T'CATG_A
Cuts at: 4136 5866 4136
Size: 1730 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 2808 3222 3602 844
Size: 1520 444 414 380 3489
Fragments arranged by size:
3489 1520 444 414 380
BsrBI CCG'CTC
Cuts at: 1394 1823 4134 1394
Size: 429 2311 3507
Fragments arranged by size:
3507 2311 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4742 4916 65
Size: 1183 3494 174 1396
Fragments arranged by size:
3494 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4891 5078 1700
Size: 110 3081 187 2869
Fragments arranged by size:
3081 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6247
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 3057 4307 5319 3057
Size: 1250 1012 3985
Fragments arranged by size:
3985 1250 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3234 794
Size: 2440 3807
BstBI TT'CG_AA
Cuts at: 5051 5051
Size: 6247
BstEII G'GTnAC_C
Cuts at: 1184 2455 3089 1184
Size: 1271 634 4342
Fragments arranged by size:
4342 1271 634
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2794 2914 3203 2248
Size: 546 120 289 5292
Fragments arranged by size:
5292 546 289 120
BstYI r'GATC_y
Cuts at: 1972 2917 3255 3303 3474 3577 3944 4329 4346
Size: 945 338 48 171 103 367 385 17
Cuts at: 4346 5798 5884 5896 6242 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 945 385 367 346 338 171
103 86 48 17 12
Bsu36I CC'TnA_GG
Cuts at: 2381 2517 2381
Size: 136 6111
BtgI C'CryG_G
Cuts at: 513 1065 2004 2121 513
Size: 552 939 117 4639
Fragments arranged by size:
4639 939 552 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5508
Fragments arranged by size:
5508 683 56
BtsI GCAGTG_nn'
Cuts at: 3214 3763 4557 4577 3214
Size: 549 794 20 4884
Fragments arranged by size:
4884 794 549 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6212
DraI TTT'AAA
Cuts at: 3551 3551
Size: 6247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5254 809
Size: 507 3938 1802
Fragments arranged by size:
3938 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2609 2922 3694
Size: 54 1425 50 275 18 779 313 772
Cuts at: 3694 4583 6212 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 779 772 313 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2922 3694 1537
Size: 275 1110 772 4090
Fragments arranged by size:
4090 1110 772 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 2499 2618 3044 4177 1353
Size: 738 408 119 426 1133 3423
Fragments arranged by size:
3423 1133 738 426 408 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4834 5208 5354 387
Size: 1064 138 3245 374 146 1280
Fragments arranged by size:
3245 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2026 2210 2560 2642 2769 2828 3019
Size: 393 394 184 350 82 127 59 191
Cuts at: 3019 3305 3401 4310 4907 5694 5962 1239
Size: 286 96 909 597 787 268 1524
Fragments arranged by size:
1524 909 787 597 394 393 350 286
268 191 184 127 96 82 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2537 2996 2537
Size: 459 5788
EcoO109I rG'GnC_Cy
Cuts at: 2353 2377 2486 2676 2979 2353
Size: 24 109 190 303 5621
Fragments arranged by size:
5621 303 190 109 24
EcoRI G'AATT_C
Cuts at: 3434 3556 3934 3434
Size: 122 378 5747
Fragments arranged by size:
5747 378 122
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 5394 1678
Size: 3716 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2949 2981 4888 4920 4962
Size: 32 135 32 1377 32 1907 32 42
Cuts at: 4962 4994 1373
Size: 32 2626
Fragments arranged by size:
2626 1907 1377 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3591 3837 669
Size: 988 281 1653 246 3079
Fragments arranged by size:
3079 1653 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6247
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KpnI G_GTAC'C
Cuts at: 2656 3572 2656
Size: 916 5331
MfeI C'AATT_G
Cuts at: 3846 3846
Size: 6247
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2751 5361 5545 1305
Size: 1446 2610 184 2007
Fragments arranged by size:
2610 2007 1446 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4425
Fragments arranged by size:
4425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2310 2462 2789 2792 4205 4564 4723
Size: 648 1144 152 327 3 1413 359 159
Cuts at: 4723 518
Size: 2042
Fragments arranged by size:
2042 1413 1144 648 359 327 159 152
3
MspA1I CmG'CkG
Cuts at: 1606 3039 3430 3663 4340 5488 5733 1606
Size: 1433 391 233 677 1148 245 2120
Fragments arranged by size:
2120 1433 1148 677 391 245 233
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4756
Fragments arranged by size:
4756 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 3605 5150 1132
Size: 97 2376 1545 2229
Fragments arranged by size:
2376 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2482 1348
Size: 1134 5113
PciI A'CATG_T
Cuts at: 5146 5146
Size: 6247
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6247
PfoI T'CCnGG_A
Cuts at: 2286 2554 2805 2913 2286
Size: 268 251 108 5620
Fragments arranged by size:
5620 268 251 108
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3017 3049 4316 4348 5829 5861 3017
Size: 32 1267 32 1481 32 3403
Fragments arranged by size:
3403 1481 1267 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2353 2377 2676 2353
Size: 24 299 5924
Fragments arranged by size:
5924 299 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 3817 3817
Size: 6247
PspXI vC'TCGA_Gb
Cuts at: 2372 2372
Size: 6247
PstI C_TGCA'G
Cuts at: 830 2076 2589 3183 3456 3599 830
Size: 1246 513 594 273 143 3478
Fragments arranged by size:
3478 1246 594 513 273 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
PvuII CAG'CTG
Cuts at: 3039 3430 3039
Size: 391 5856
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 6247
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SapI GCTCTTCn'nnn_
Cuts at: 2618 2618
Size: 6247
SbfI CC_TGCA'GG
Cuts at: 3456 3599 3456
Size: 143 6104
ScaI AGT'ACT
Cuts at: 2843 4495 6207 2843
Size: 1652 1712 2883
Fragments arranged by size:
2883 1712 1652
SfcI C'TryA_G
Cuts at: 826 1045 2072 2473 2585 3179 3452 3595 4730
Size: 219 1027 401 112 594 273 143 1135
Cuts at: 4730 5411 5602 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 594 401 273 219
191 143 112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 3574 1872
Size: 1702 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2372 4351 5252 5514 820
Size: 197 926 429 1979 901 262 1553
Fragments arranged by size:
1979 1553 926 901 429 262 197
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6247
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 4171 5
Size: 47 4119 2081
Fragments arranged by size:
4119 2081 47
StuI AGG'CCT
Cuts at: 1517 2543 1517
Size: 1026 5221
StyI C'CwwG_G
Cuts at: 513 1065 2004 3439 3686 513
Size: 552 939 1435 247 3074
Fragments arranged by size:
3074 1435 939 552 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4274 4291 4444 4629 5074 325
Size: 1255 2694 17 153 185 445 1498
Fragments arranged by size:
2694 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 4493 6205
Size: 274 80 33 51 1443 864 1652 1712
Cuts at: 6205 96
Size: 138
Fragments arranged by size:
1712 1652 1443 864 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3048 3080 6207 6239 3048
Size: 32 3127 32 3056
Fragments arranged by size:
3127 3056 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6247
XbaI T'CTAG_A
Cuts at: 3583 3583
Size: 6247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2159 2938 2159
Size: 779 5468
XhoI C'TCGA_G
Cuts at: 1943 2372 1943
Size: 429 5818
XmaI C'CCGG_G
Cuts at: 1870 3572 1870
Size: 1702 4545
XmnI GAAnn'nnTTC
Cuts at: 4376 4376
Size: 6247
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4055 276
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PsrI SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI