Restriction map for
pFN21AE5626
(Circular) (Six-base) MAPSORT of: pFN21AE5626.seq Check: 4264 from: 1 to: 6154
pFN21AE5626 6154 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2415 2415
Size: 6154
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3964 278
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3497 1937
Size: 1560 4594
Acc65I G'GTAC_C
Cuts at: 3475 3475
Size: 6154
AclI AA'CG_TT
Cuts at: 1563 4281 4654 1563
Size: 2718 373 3063
Fragments arranged by size:
3063 2718 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3377 4217 5601 5869 1632
Size: 1745 840 1384 268 1917
Fragments arranged by size:
1917 1745 1384 840 268
AfeI AGC'GCT
Cuts at: 2669 2726 2669
Size: 57 6097
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5957
AflIII A'CryG_T
Cuts at: 1203 2368 2772 5053 6030 1203
Size: 1165 404 2281 977 1327
Fragments arranged by size:
2281 1327 1165 977 404
AgeI A'CCGG_T
Cuts at: 4985 4985
Size: 6154
AhdI GACnn_n'nnGTC
Cuts at: 4883 4883
Size: 6154
AleI CACnn'nnGTG
Cuts at: 1166 2084 3056 1166
Size: 918 972 4264
Fragments arranged by size:
4264 972 918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2822 1184
Size: 1638 4516
AlwNI CAG_nnn'CTG
Cuts at: 2194 2347 2551 2599 2794 3448 3897 5469 2194
Size: 153 204 48 195 654 449 1572 2879
Fragments arranged by size:
2879 1572 654 449 204 195 153 48
ApaLI G'TGCA_C
Cuts at: 3083 4211 5367 3083
Size: 1128 1156 3870
Fragments arranged by size:
3870 1156 1128
ApoI r'AATT_y
Cuts at: 1493 2813 3463 3689 3841 5950 1493
Size: 1320 650 226 152 2109 1697
Fragments arranged by size:
2109 1697 1320 650 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2931 2981 3479 1870
Size: 73 81 907 50 498 4545
Fragments arranged by size:
4545 907 498 81 73 50
BamHI G'GATC_C
Cuts at: 2203 2904 3484 3851 2203
Size: 701 580 367 4506
Fragments arranged by size:
4506 701 580 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2391 2680 3475 4930 618
Size: 325 217 627 604 289 795 1455 1842
Fragments arranged by size:
1842 1455 795 627 604 325 289 217
BanII G_rGCy'C
Cuts at: 1391 2025 2355 2988 3473 1391
Size: 634 330 633 485 4072
Fragments arranged by size:
4072 634 633 485 330
BbeI G_GCGC'C
Cuts at: 2395 2395
Size: 6154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2165 928
Size: 971 266 4917
Fragments arranged by size:
4917 971 266
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2299 2333 4345 4379 1903
Size: 34 362 34 2012 34 3678
Fragments arranged by size:
3678 2012 362 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4046 5256 4046
Size: 1210 4944
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2296 2522 4765
Size: 107 122 71 631 605 624 226 2243
Cuts at: 4765 136
Size: 1525
Fragments arranged by size:
2243 1525 631 624 605 226 122 107
71
BglII A'GATC_T
Cuts at: 2994 6149 2994
Size: 3155 2999
BlpI GC'TnA_GC
Cuts at: 3297 3571 3297
Size: 274 5880
Bme1580I G_kGCm'C
Cuts at: 1790 3087 4215 5371 1790
Size: 1297 1128 1156 2573
Fragments arranged by size:
2573 1297 1156 1128
BmgBI CAC'GTC
Cuts at: 1318 2923 1318
Size: 1605 4549
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3425 3885 4843
Size: 416 53 83 399 24 2008 460 958
Cuts at: 4843 442
Size: 1753
Fragments arranged by size:
2008 1753 958 460 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6154
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3142 3142
Size: 6154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2369 2531 3137 3221 3278 3299 4814
Size: 896 234 162 606 84 57 21 1515
Cuts at: 4814 1239
Size: 2579
Fragments arranged by size:
2579 1515 896 606 234 162 84 57
21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4279 5144 5442 4279
Size: 865 298 4991
Fragments arranged by size:
4991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4817 882
Size: 617 3318 2219
Fragments arranged by size:
3318 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2371 2775 3945 493
Size: 711 1167 404 1170 2702
Fragments arranged by size:
2702 1170 1167 711 404
BsaBI GATnn'nnATC
Cuts at: 2909 2999 6148 2909
Size: 90 3149 2915
Fragments arranged by size:
3149 2915 90
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2392 2700 3394 3961
Size: 53 83 186 936 859 308 694 567
Cuts at: 3961 4343 4978 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 859 694 635 567 382 308
186 83 53
BsaWI w'CCGG_w
Cuts at: 2901 4587 4985 5259 5406 5817 2901
Size: 1686 398 274 147 411 3238
Fragments arranged by size:
3238 1686 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2912 2942 1178
Size: 30 165 30 1509 30 4390
Fragments arranged by size:
4390 1509 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3110 3134 3362 1186
Size: 1924 24 228 3978
Fragments arranged by size:
3978 1924 228 24
BseYI C'CCAG_C
Cuts at: 2062 5357 5853 2062
Size: 3295 496 2363
Fragments arranged by size:
3295 2363 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 2473 2822 3048 1163
Size: 573 372 365 349 226 4269
Fragments arranged by size:
4269 573 372 365 349 226
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3016 3604 4365 5393 1540
Size: 275 186 1015 588 761 1028 2301
Fragments arranged by size:
2301 1028 1015 761 588 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2355 2648 2742 3087 4215 4300 5371 2025
Size: 330 293 94 345 1128 85 1071 2808
Fragments arranged by size:
2808 1128 1071 345 330 293 94 85
BsmI GAATG_Cn'
Cuts at: 3665 3758 3665
Size: 93 6061
BsmBI CGTCTCn'nnnn_
Cuts at: 3344 3344
Size: 6154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2355 2648 2742 2988 3087 3473
Size: 399 235 330 293 94 246 99 386
Cuts at: 3473 4215 4300 5371 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 386 330 293 246
235 99 94 85
BspEI T'CCGG_A
Cuts at: 5817 5817
Size: 6154
BspHI T'CATG_A
Cuts at: 4043 5773 4043
Size: 1730 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2415 2682 2792 3509 844
Size: 1571 267 110 717 3489
Fragments arranged by size:
3489 1571 717 267 110
BsrBI CCG'CTC
Cuts at: 1394 1823 4041 1394
Size: 429 2218 3507
Fragments arranged by size:
3507 2218 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4649 4823 65
Size: 1183 3401 174 1396
Fragments arranged by size:
3401 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2469 2533 3324 4798 4985 1700
Size: 110 659 64 791 1474 187 2869
Fragments arranged by size:
2869 1474 791 659 187 110 64
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6154
BssSI C'ACGA_G
Cuts at: 2042 2741 4214 5226 2042
Size: 699 1473 1012 2970
Fragments arranged by size:
2970 1473 1012 699
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2220 2551 2784 794
Size: 1426 331 233 4164
Fragments arranged by size:
4164 1426 331 233
BstBI TT'CG_AA
Cuts at: 4958 4958
Size: 6154
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6154
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 3055 2248
Size: 807 5347
BstYI r'GATC_y
Cuts at: 1972 2203 2439 2904 2994 3153 3484 3851 4236
Size: 231 236 465 90 159 331 367 385
Cuts at: 4236 4253 5705 5791 5803 6149 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 465 385 367 346 331 236
231 159 90 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2502 2649 2829 3129 513
Size: 552 939 498 147 180 300 3538
Fragments arranged by size:
3538 939 552 498 300 180 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2362 487
Size: 683 56 1136 4279
Fragments arranged by size:
4279 1136 683 56
BtsI GCAGTG_nn'
Cuts at: 2158 2549 3670 4464 4484 2158
Size: 391 1121 794 20 3828
Fragments arranged by size:
3828 1121 794 391 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2904 2939 529
Size: 35 2340 35 3744
Fragments arranged by size:
3744 2340 35 35
DraI TTT'AAA
Cuts at: 3458 3458
Size: 6154
DraIII CAC_nnn'GTG
Cuts at: 3082 3082
Size: 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5161 809
Size: 507 3845 1802
Fragments arranged by size:
3845 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2601 3013 3303
Size: 54 1425 50 275 18 771 412 290
Cuts at: 3303 3382 3601 4490 6119 8
Size: 79 219 889 1629 43
Fragments arranged by size:
1629 1425 889 771 412 290 275 219
79 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3013 3601 1537
Size: 275 1201 588 4090
Fragments arranged by size:
4090 1201 588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3129 3175 4084 1353
Size: 1776 46 909 3423
Fragments arranged by size:
3423 1776 909 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4741 5115 5261 387
Size: 1064 138 3152 374 146 1280
Fragments arranged by size:
3152 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2023 2353 2023
Size: 330 5824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2369 2531 3137 3221 3278 3299
Size: 393 503 234 162 606 84 57 21
Cuts at: 3299 3377 4217 4814 5601 5869 1239
Size: 78 840 597 787 268 1524
Fragments arranged by size:
1524 840 787 606 597 503 393 268
234 162 84 78 57 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3161 3161
Size: 6154
EcoO109I rG'GnC_Cy
Cuts at: 2278 2635 2935 3268 3417 3445 2278
Size: 357 300 333 149 28 4987
Fragments arranged by size:
4987 357 333 300 149 28
EcoRI G'AATT_C
Cuts at: 3463 3841 3463
Size: 378 5776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 6154
FspI TGC'GCA
Cuts at: 2411 2810 4660 2411
Size: 399 1850 3905
Fragments arranged by size:
3905 1850 399
HaeII r_GCGC'y
Cuts at: 1678 2348 2395 2565 2671 2728 3331 5301 1678
Size: 670 47 170 106 57 603 1970 2531
Fragments arranged by size:
2531 1970 670 603 170 106 57 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2735 2767 2912 2944 4795
Size: 32 135 32 1163 32 145 32 1851
Cuts at: 4795 4827 4869 4901 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1851 1163 145 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3169 3498 3744 669
Size: 988 281 1231 329 246 3079
Fragments arranged by size:
3079 1231 988 329 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6154
HpaI GTT'AAC
Cuts at: 3744 3744
Size: 6154
KasI G'GCGC_C
Cuts at: 2391 2391
Size: 6154
KpnI G_GTAC'C
Cuts at: 3479 3479
Size: 6154
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2442 5268 5452 1305
Size: 1137 2826 184 2007
Fragments arranged by size:
2826 2007 1137 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2603 3305 3384 10
Size: 54 1425 343 771 702 79 2780
Fragments arranged by size:
2780 1425 771 702 343 79 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2330 3056 4112 4471 4630 518
Size: 648 918 246 726 1056 359 159 2042
Fragments arranged by size:
2042 1056 918 726 648 359 246 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2659 3250 3570 4247 5395 5640 1606
Size: 456 597 591 320 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 597 591 456 320 245
NaeI GCC'GGC
Cuts at: 2535 2535
Size: 6154
NarI GG'CG_CC
Cuts at: 2392 2392
Size: 6154
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4663
Fragments arranged by size:
4663 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6154
NgoMIV G'CCGG_C
Cuts at: 2533 2533
Size: 6154
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6154
NotI GC'GGCC_GC
Cuts at: 3601 3601
Size: 6154
NspI r_CATG'y
Cuts at: 1132 1229 3512 5057 1132
Size: 97 2283 1545 2229
Fragments arranged by size:
2283 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3442 1348
Size: 2094 4060
PciI A'CATG_T
Cuts at: 5053 5053
Size: 6154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2347 3448 1108
Size: 1239 1101 3814
Fragments arranged by size:
3814 1239 1101
PfoI T'CCnGG_A
Cuts at: 2112 2583 2730 3255 2112
Size: 471 147 525 5011
Fragments arranged by size:
5011 525 471 147
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PmlI CAC'GTG
Cuts at: 2371 2775 2371
Size: 404 5750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4223 4255 5736 5768 4223
Size: 32 1481 32 4609
Fragments arranged by size:
4609 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2278 3268 3417 2278
Size: 990 149 5015
Fragments arranged by size:
5015 990 149
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6154
PsiI TTA'TAA
Cuts at: 3724 3724
Size: 6154
PstI C_TGCA'G
Cuts at: 830 2595 3506 830
Size: 1765 911 3478
Fragments arranged by size:
3478 1765 911
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
PvuII CAG'CTG
Cuts at: 2062 2659 3250 2062
Size: 597 591 4966
Fragments arranged by size:
4966 597 591
SacI G_AGCT'C
Cuts at: 2025 2355 2025
Size: 330 5824
SalI G'TCGA_C
Cuts at: 1936 3496 1936
Size: 1560 4594
SanDI GG'GwC_CC
Cuts at: 3417 3417
Size: 6154
SbfI CC_TGCA'GG
Cuts at: 3506 3506
Size: 6154
ScaI AGT'ACT
Cuts at: 4402 6114 4402
Size: 1712 4442
SexAI A'CCwGG_T
Cuts at: 2706 3078 2706
Size: 372 5782
SfcI C'TryA_G
Cuts at: 826 1045 2591 3149 3502 4637 5318 5509 826
Size: 219 1546 558 353 1135 681 191 1471
Fragments arranged by size:
1546 1471 1135 681 558 353 219 191
SfoI GGC'GCC
Cuts at: 2393 2393
Size: 6154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6154
SmaI CCC'GGG
Cuts at: 1872 2933 2983 3481 1872
Size: 1061 50 498 4545
Fragments arranged by size:
4545 1061 498 50
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4258 5159 5421 820
Size: 197 926 2315 901 262 1553
Fragments arranged by size:
2315 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6154
SphI G_CATG'C
Cuts at: 1132 3512 1132
Size: 2380 3774
SspI AAT'ATT
Cuts at: 5 52 4078 5
Size: 47 4026 2081
Fragments arranged by size:
4026 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6154
StyI C'CwwG_G
Cuts at: 513 1065 2004 3593 513
Size: 552 939 1589 3074
Fragments arranged by size:
3074 1589 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2251 2588 3415 4181 4198 4351 4536
Size: 1255 671 337 827 766 17 153 185
Cuts at: 4536 4981 325
Size: 445 1498
Fragments arranged by size:
1498 1255 827 766 671 445 337 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4400 6112 96
Size: 274 80 33 51 1443 2423 1712 138
Fragments arranged by size:
2423 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3010 3042 6114 6146 3010
Size: 32 3072 32 3018
Fragments arranged by size:
3072 3018 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3409 1316
Size: 2093 4061
XbaI T'CTAG_A
Cuts at: 3490 3490
Size: 6154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2245 2245
Size: 6154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6154
XmaI C'CCGG_G
Cuts at: 1870 2931 2981 3479 1870
Size: 1061 50 498 4545
Fragments arranged by size:
4545 1061 498 50
XmnI GAAnn'nnTTC
Cuts at: 4283 4283
Size: 6154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3962 276
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacII SapI SfiI
SrfI SwaI