Restriction map for
pFN21AE5619
(Circular) (Six-base) MAPSORT of: pFN21AE5619.seq Check: 6778 from: 1 to: 6148
pFN21AE5619 6148 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3491 1937
Size: 1554 4594
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2490 2539 4211 5595 5863 1632
Size: 858 49 1672 1384 268 1917
Fragments arranged by size:
1917 1672 1384 858 268 49
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 2194 3084 5047 6024 1203
Size: 991 890 1963 977 1327
Fragments arranged by size:
1963 1327 991 977 890
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 2343 1166
Size: 1177 4971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3219 1184
Size: 2035 4113
AlwNI CAG_nnn'CTG
Cuts at: 2147 3891 5463 2147
Size: 1744 1572 2832
Fragments arranged by size:
2832 1744 1572
ApaI G_GGCC'C
Cuts at: 2539 2539
Size: 6148
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 2134 2960 3457 3683 3835 5944 1493
Size: 641 826 497 226 152 2109 1697
Fragments arranged by size:
2109 1697 826 641 497 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2709 4702 160
Size: 872 1677 1993 1606
Fragments arranged by size:
1993 1677 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 3473 1870
Size: 73 1530 4545
Fragments arranged by size:
4545 1530 73
BamHI G'GATC_C
Cuts at: 3282 3478 3845 3282
Size: 196 367 5585
Fragments arranged by size:
5585 367 196
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2657 3469 4924 618
Size: 325 217 627 870 812 1455 1842
Fragments arranged by size:
1842 1455 870 812 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2539 2762 3467 1391
Size: 1148 223 705 4072
Fragments arranged by size:
4072 1148 705 223
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2197 3387 928
Size: 971 298 1190 3689
Fragments arranged by size:
3689 1190 971 298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 1903
Size: 34 2402 34 3678
Fragments arranged by size:
3678 2402 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2222 4040 5250 2222
Size: 1818 1210 3120
Fragments arranged by size:
3120 1818 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4759 136
Size: 107 122 71 631 605 3087 1525
Fragments arranged by size:
3087 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6143 6143
Size: 6148
BlpI GC'TnA_GC
Cuts at: 3565 3565
Size: 6148
Bme1580I G_kGCm'C
Cuts at: 1790 2539 4209 5365 1790
Size: 749 1670 1156 2573
Fragments arranged by size:
2573 1670 1156 749
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3879 4837 442
Size: 416 53 83 399 24 2462 958 1753
Fragments arranged by size:
2462 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2496 4808 1239
Size: 848 409 2312 2579
Fragments arranged by size:
2579 2312 848 409
Bpu10I CC'TnA_GC
Cuts at: 3024 3024
Size: 6148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2597 2721 4273 5138 5436 2597
Size: 124 1552 865 298 3309
Fragments arranged by size:
3309 1552 865 298 124
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4811 882
Size: 617 3312 2219
Fragments arranged by size:
3312 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2197 3299 3939 493
Size: 711 993 1102 640 2702
Fragments arranged by size:
2702 1102 993 711 640
BsaBI GATnn'nnATC
Cuts at: 2855 6142 2855
Size: 3287 2861
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3955 4337 4972 275
Size: 53 83 186 936 2422 382 635 1451
Fragments arranged by size:
2422 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4581 4979 5253 5400 5811 4581
Size: 398 274 147 411 4918
Fragments arranged by size:
4918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5923
Fragments arranged by size:
5923 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2465 1186
Size: 1279 4869
BseYI C'CCAG_C
Cuts at: 2223 5351 5847 2223
Size: 3128 496 2524
Fragments arranged by size:
3128 2524 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5575
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 4359 5387 1540
Size: 275 186 1597 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2182 3318 4209 4294 5365 2182
Size: 1136 891 85 1071 2965
Fragments arranged by size:
2965 1136 1071 891 85
BsmI GAATG_Cn'
Cuts at: 2822 3659 3752 2822
Size: 837 93 5218
Fragments arranged by size:
5218 837 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2182 2539 2762 3318 3467 4209 4294
Size: 399 392 357 223 556 149 742 85
Cuts at: 4294 5365 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 556 399 392 357 223
149 85
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3503 844
Size: 2659 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2581 4035 1394
Size: 429 758 1454 3507
Fragments arranged by size:
3507 1454 758 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4643 4817 65
Size: 1183 3395 174 1396
Fragments arranged by size:
3395 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4792 4979 1700
Size: 110 2982 187 2869
Fragments arranged by size:
2982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 2604 4208 5220 2604
Size: 1604 1012 3532
Fragments arranged by size:
3532 1604 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2077 794
Size: 1283 4865
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6148
BstYI r'GATC_y
Cuts at: 1972 2475 3006 3282 3478 3845 4230 4247 5699
Size: 503 531 276 196 367 385 17 1452
Cuts at: 5699 5785 5797 6143 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 531 503 385 367 346 276
196 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2628 513
Size: 552 939 624 4033
Fragments arranged by size:
4033 939 624 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 3664 4458 4478 3664
Size: 794 20 5334
Fragments arranged by size:
5334 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3038 3073 529
Size: 35 2474 35 3604
Fragments arranged by size:
3604 2474 35 35
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5155 809
Size: 507 3839 1802
Fragments arranged by size:
3839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2185 2815 2872
Size: 54 1425 50 275 18 355 630 57
Cuts at: 2872 3595 4484 6113 8
Size: 723 889 1629 43
Fragments arranged by size:
1629 1425 889 723 630 355 275 57
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 1537
Size: 275 1783 4090
Fragments arranged by size:
4090 1783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2268 2466 3404 4078 1353
Size: 915 198 938 674 3423
Fragments arranged by size:
3423 938 915 674 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2490 2496 2539 4211 4808 5595
Size: 393 455 403 6 43 1672 597 787
Cuts at: 5595 5863 1239
Size: 268 1524
Fragments arranged by size:
1672 1524 787 597 455 403 393 268
43 6
EcoO109I rG'GnC_Cy
Cuts at: 2536 2536
Size: 6148
EcoRI G'AATT_C
Cuts at: 3457 3835 3457
Size: 378 5770
FspI TGC'GCA
Cuts at: 3230 4654 3230
Size: 1424 4724
FspAI rTGC'GCAy
Cuts at: 3230 3230
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 5295 1678
Size: 3617 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2370 2387 2402 2419 4789
Size: 32 135 32 798 17 15 17 2370
Cuts at: 4789 4821 4863 4895 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2370 798 135 42 32 32 32
32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3424 3492 3738 669
Size: 988 281 1486 68 246 3079
Fragments arranged by size:
3079 1486 988 281 246 68
HindIII A'AGCT_T
Cuts at: 748 2551 748
Size: 1803 4345
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2355 5262 5446 1305
Size: 1050 2907 184 2007
Fragments arranged by size:
2907 2007 1050 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2817 2874 10
Size: 54 1425 343 985 57 3284
Fragments arranged by size:
3284 1425 985 343 57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 4106 4465 4624 518
Size: 648 1177 1763 359 159 2042
Fragments arranged by size:
2042 1763 1177 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2571 3564 4241 5389 5634 1606
Size: 965 993 677 1148 245 2120
Fragments arranged by size:
2120 1148 993 965 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2628 513
Size: 552 939 624 4033
Fragments arranged by size:
4033 939 624 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 3088 3506 5051 1132
Size: 97 1859 418 1545 2229
Fragments arranged by size:
2229 1859 1545 418 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6148
PciI A'CATG_T
Cuts at: 3084 5047 3084
Size: 1963 4185
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2881 3271 1108
Size: 1773 390 3985
Fragments arranged by size:
3985 1773 390
PfoI T'CCnGG_A
Cuts at: 3278 3278
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PspOMI G'GGCC_C
Cuts at: 2535 2535
Size: 6148
PstI C_TGCA'G
Cuts at: 830 3500 830
Size: 2670 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2571 2571
Size: 6148
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 4396 6108 4396
Size: 1712 4436
SfcI C'TryA_G
Cuts at: 826 1045 2541 2955 3496 4631 5312 5503 826
Size: 219 1496 414 541 1135 681 191 1471
Fragments arranged by size:
1496 1471 1135 681 541 414 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2576 2736 4252 5153 5415 820
Size: 197 926 633 160 1516 901 262 1553
Fragments arranged by size:
1553 1516 926 901 633 262 197 160
SnaBI TAC'GTA
Cuts at: 493 3299 3939 493
Size: 2806 640 2702
Fragments arranged by size:
2806 2702 640
SpeI A'CTAG_T
Cuts at: 152 2308 152
Size: 2156 3992
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 3037 4072 5
Size: 47 2985 1035 2081
Fragments arranged by size:
2985 2081 1035 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6148
StyI C'CwwG_G
Cuts at: 513 1065 2004 2414 2628 2875 3265 3587 513
Size: 552 939 410 214 247 390 322 3074
Fragments arranged by size:
3074 939 552 410 390 322 247 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2675 3333 4175 4192 4345 4530 4975
Size: 1255 1095 658 842 17 153 185 445
Cuts at: 4975 325
Size: 1498
Fragments arranged by size:
1498 1255 1095 842 658 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2740 4394 6106
Size: 274 80 33 51 1443 763 1654 1712
Cuts at: 6106 96
Size: 138
Fragments arranged by size:
1712 1654 1443 763 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3312 3344 6108 6140 3312
Size: 32 2764 32 3320
Fragments arranged by size:
3320 2764 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6148
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 4277 4277
Size: 6148
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI