Restriction map for
pFN21AE5617
(Circular) (Six-base) MAPSORT of: pFN21AE5617.seq Check: 941 from: 1 to: 6343
pFN21AE5617 6343 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4153 278
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2178 3686 1937
Size: 241 1508 4594
Fragments arranged by size:
4594 1508 241
Acc65I G'GTAC_C
Cuts at: 3664 3664
Size: 6343
AclI AA'CG_TT
Cuts at: 1563 4470 4843 1563
Size: 2907 373 3063
Fragments arranged by size:
3063 2907 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2346 2715 2928 3618 4406 5790 6058 1632
Size: 714 369 213 690 788 1384 268 1917
Fragments arranged by size:
1917 1384 788 714 690 369 268 213
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6146
AflIII A'CryG_T
Cuts at: 1203 3087 5242 6219 1203
Size: 1884 2155 977 1327
Fragments arranged by size:
2155 1884 1327 977
AgeI A'CCGG_T
Cuts at: 5174 5174
Size: 6343
AhdI GACnn_n'nnGTC
Cuts at: 5072 5072
Size: 6343
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6343
AlwNI CAG_nnn'CTG
Cuts at: 2579 2738 3454 4086 5658 2579
Size: 159 716 632 1572 3264
Fragments arranged by size:
3264 1572 716 632 159
ApaI G_GGCC'C
Cuts at: 2507 2507
Size: 6343
ApaLI G'TGCA_C
Cuts at: 4400 5556 4400
Size: 1156 5187
ApoI r'AATT_y
Cuts at: 1493 3305 3652 3878 4030 6139 1493
Size: 1812 347 226 152 2109 1697
Fragments arranged by size:
2109 1812 1697 347 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4897 160
Size: 872 3865 1606
Fragments arranged by size:
3865 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
AvaI C'yCGr_G
Cuts at: 1870 1943 2751 3668 1870
Size: 73 808 917 4545
Fragments arranged by size:
4545 917 808 73
BamHI G'GATC_C
Cuts at: 3673 4040 3673
Size: 367 5976
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2060 2944 3457 3664
Size: 325 217 627 222 51 884 513 207
Cuts at: 3664 5119 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 884 627 513 325 222 217
207 51
BanII G_rGCy'C
Cuts at: 1391 2507 3543 3662 1391
Size: 1116 1036 119 4072
Fragments arranged by size:
4072 1116 1036 119
BbeI G_GCGC'C
Cuts at: 2064 2064
Size: 6343
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2826 928
Size: 971 927 4445
Fragments arranged by size:
4445 971 927
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4534 4568 1903
Size: 34 2597 34 3678
Fragments arranged by size:
3678 2597 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4235 5445 4235
Size: 1210 5133
BclI T'GATC_A
Cuts at: 1548 3097 1548
Size: 1549 4794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2300 4954 136
Size: 107 122 71 631 605 628 2654 1525
Fragments arranged by size:
2654 1525 631 628 605 122 107 71
BglII A'GATC_T
Cuts at: 6338 6338
Size: 6343
BlpI GC'TnA_GC
Cuts at: 3760 3760
Size: 6343
Bme1580I G_kGCm'C
Cuts at: 1790 2507 4404 5560 1790
Size: 717 1897 1156 2573
Fragments arranged by size:
2573 1897 1156 717
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6343
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4074 5032 442
Size: 416 53 83 399 24 2657 958 1753
Fragments arranged by size:
2657 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6343
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2992 3598 2992
Size: 606 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2544 3178 3527 5003 1239
Size: 1305 634 349 1476 2579
Fragments arranged by size:
2579 1476 1305 634 349
Bpu10I CC'TnA_GC
Cuts at: 2145 2145
Size: 6343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4468 5333 5631 4468
Size: 865 298 5180
Fragments arranged by size:
5180 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5006 882
Size: 617 3507 2219
Fragments arranged by size:
3507 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4134 493
Size: 711 2930 2702
Fragments arranged by size:
2930 2702 711
BsaBI GATnn'nnATC
Cuts at: 6337 6337
Size: 6343
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2310 4150 4532
Size: 53 83 186 936 528 249 1840 382
Cuts at: 4532 5167 275
Size: 635 1451
Fragments arranged by size:
1840 1451 936 635 528 382 249 186
83 53
BsaWI w'CCGG_w
Cuts at: 4776 5174 5448 5595 6006 4776
Size: 398 274 147 411 5113
Fragments arranged by size:
5113 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 3398 3428 1178
Size: 30 165 30 672 30 1293 30 4093
Fragments arranged by size:
4093 1293 672 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 2879 2915 2981 3587 1186
Size: 1252 441 36 66 606 3942
Fragments arranged by size:
3942 1252 606 441 66 36
BseYI C'CCAG_C
Cuts at: 2022 2714 5546 6042 2022
Size: 692 2832 496 2323
Fragments arranged by size:
2832 2323 692 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2415 1163
Size: 573 679 5091
Fragments arranged by size:
5091 679 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3793 4554 5582 1540
Size: 275 186 1792 761 1028 2301
Fragments arranged by size:
2301 1792 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2045 3543 3582 4404 4489 5560 2045
Size: 1498 39 822 85 1071 2828
Fragments arranged by size:
2828 1498 1071 822 85 39
BsmI GAATG_Cn'
Cuts at: 2697 2837 3145 3854 3947 2697
Size: 140 308 709 93 5093
Fragments arranged by size:
5093 709 308 140 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2119 3633 2119
Size: 1514 4829
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2507 3543 3582 3662 4404 4489
Size: 399 255 462 1036 39 80 742 85
Cuts at: 4489 5560 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1036 742 462 399 255 85
80 39
BspEI T'CCGG_A
Cuts at: 6006 6006
Size: 6343
BspHI T'CATG_A
Cuts at: 4232 5962 4232
Size: 1730 4613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3243 3509 3698 844
Size: 2399 266 189 3489
Fragments arranged by size:
3489 2399 266 189
BsrBI CCG'CTC
Cuts at: 1394 1823 4230 1394
Size: 429 2407 3507
Fragments arranged by size:
3507 2407 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3315 4838 5012 65
Size: 1183 2067 1523 174 1396
Fragments arranged by size:
2067 1523 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4987 5174 1700
Size: 110 3177 187 2869
Fragments arranged by size:
3177 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6343
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6343
BssSI C'ACGA_G
Cuts at: 4403 5415 4403
Size: 1012 5331
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2354 794
Size: 1560 4783
BstBI TT'CG_AA
Cuts at: 5147 5147
Size: 6343
BstEII G'GTnAC_C
Cuts at: 1184 3154 1184
Size: 1970 4373
BstYI r'GATC_y
Cuts at: 1972 3673 4040 4425 4442 5894 5980 5992 6338
Size: 1701 367 385 17 1452 86 12 346
Cuts at: 6338 1972
Size: 1977
Fragments arranged by size:
1977 1701 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2580 3243 2580
Size: 663 5680
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 3159 513
Size: 552 939 114 1041 3697
Fragments arranged by size:
3697 1041 939 552 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5604
Fragments arranged by size:
5604 683 56
BtsI GCAGTG_nn'
Cuts at: 2432 2866 3859 4653 4673 2432
Size: 434 993 794 20 4102
Fragments arranged by size:
4102 993 794 434 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6343
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2979 3014 529
Size: 35 2415 35 3858
Fragments arranged by size:
3858 2415 35 35
DraI TTT'AAA
Cuts at: 3647 3647
Size: 6343
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5350 809
Size: 507 4034 1802
Fragments arranged by size:
4034 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3790 4679 6308
Size: 54 1425 50 275 18 1960 889 1629
Cuts at: 6308 8
Size: 43
Fragments arranged by size:
1960 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3790 1537
Size: 275 1978 4090
Fragments arranged by size:
4090 1978 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2439 2460 2734 2808 2838 2904 2928 3429
Size: 1086 21 274 74 30 66 24 501
Cuts at: 3429 4273 1353
Size: 844 3423
Fragments arranged by size:
3423 1086 844 501 274 74 66 30
24 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2083 2819 4930 5304 5450 387
Size: 1064 138 494 736 2111 374 146 1280
Fragments arranged by size:
2111 1280 1064 736 494 374 146 138
EcoICRI GAG'CTC
Cuts at: 3541 3541
Size: 6343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2346 2544 2715 2928 3178 3527 3618
Size: 393 714 198 171 213 250 349 91
Cuts at: 3618 4406 5003 5790 6058 1239
Size: 788 597 787 268 1524
Fragments arranged by size:
1524 788 787 714 597 393 349 268
250 213 198 171 91
EcoO109I rG'GnC_Cy
Cuts at: 2503 2504 2550 2557 2576 3181 2503
Size: 1 46 7 19 605 5665
Fragments arranged by size:
5665 605 46 19 7 1
EcoRI G'AATT_C
Cuts at: 3305 3652 4030 3305
Size: 347 378 5618
Fragments arranged by size:
5618 378 347
EcoRV GAT'ATC
Cuts at: 2236 2236
Size: 6343
FspI TGC'GCA
Cuts at: 4849 4849
Size: 6343
HaeII r_GCGC'y
Cuts at: 1678 2064 5490 1678
Size: 386 3426 2531
Fragments arranged by size:
3426 2531 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2777 2809 4984 5016 5058
Size: 32 135 32 1205 32 2175 32 42
Cuts at: 5058 5090 1373
Size: 32 2626
Fragments arranged by size:
2626 2175 1205 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3175 3687 3933 669
Size: 988 281 1237 512 246 3079
Fragments arranged by size:
3079 1237 988 512 281 246
HindIII A'AGCT_T
Cuts at: 748 3078 748
Size: 2330 4013
HpaI GTT'AAC
Cuts at: 3933 3933
Size: 6343
KasI G'GCGC_C
Cuts at: 2060 2060
Size: 6343
KpnI G_GTAC'C
Cuts at: 3668 3668
Size: 6343
MfeI C'AATT_G
Cuts at: 3942 3942
Size: 6343
MluI A'CGCG_T
Cuts at: 6219 6219
Size: 6343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2676 5457 5641 1305
Size: 1371 2781 184 2007
Fragments arranged by size:
2781 2007 1371 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4521
Fragments arranged by size:
4521 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3278 4301 4660 4819 518
Size: 648 2112 1023 359 159 2042
Fragments arranged by size:
2112 2042 1023 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2996 3759 4436 5584 5829 1606
Size: 471 919 763 677 1148 245 2120
Fragments arranged by size:
2120 1148 919 763 677 471 245
NarI GG'CG_CC
Cuts at: 2061 2061
Size: 6343
NcoI C'CATG_G
Cuts at: 513 1065 2004 2118 3159 513
Size: 552 939 114 1041 3697
Fragments arranged by size:
3697 1041 939 552 114
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6343
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6343
NotI GC'GGCC_GC
Cuts at: 3790 3790
Size: 6343
NspI r_CATG'y
Cuts at: 1132 1229 3091 3701 5246 1132
Size: 97 1862 610 1545 2229
Fragments arranged by size:
2229 1862 1545 610 97
PasI CC'CwG_GG
Cuts at: 1348 2554 1348
Size: 1206 5137
PciI A'CATG_T
Cuts at: 3087 5242 3087
Size: 2155 4188
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3554 1108
Size: 2446 3897
PmeI GTTT'AAAC
Cuts at: 3647 3647
Size: 6343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 5925 5957 4412
Size: 32 1481 32 4798
Fragments arranged by size:
4798 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2550 2576 2550
Size: 26 6317
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6343
PsiI TTA'TAA
Cuts at: 3913 3913
Size: 6343
PspOMI G'GGCC_C
Cuts at: 2503 2503
Size: 6343
PspXI vC'TCGA_Gb
Cuts at: 2751 2751
Size: 6343
PstI C_TGCA'G
Cuts at: 830 3695 830
Size: 2865 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6343
SacI G_AGCT'C
Cuts at: 3543 3543
Size: 6343
SalI G'TCGA_C
Cuts at: 1936 3685 1936
Size: 1749 4594
SanDI GG'GwC_CC
Cuts at: 2550 2550
Size: 6343
SapI GCTCTTCn'nnn_
Cuts at: 2460 2734 3429 2460
Size: 274 695 5374
Fragments arranged by size:
5374 695 274
SbfI CC_TGCA'GG
Cuts at: 3695 3695
Size: 6343
ScaI AGT'ACT
Cuts at: 4591 6303 4591
Size: 1712 4631
SfcI C'TryA_G
Cuts at: 826 1045 2273 2854 3691 4826 5507 5698 826
Size: 219 1228 581 837 1135 681 191 1471
Fragments arranged by size:
1471 1228 1135 837 681 581 219 191
SfoI GGC'GCC
Cuts at: 2062 2062
Size: 6343
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6343
SmaI CCC'GGG
Cuts at: 1872 3670 1872
Size: 1798 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2751 4447 5348 5610 820
Size: 197 926 808 1696 901 262 1553
Fragments arranged by size:
1696 1553 926 901 808 262 197
SnaBI TAC'GTA
Cuts at: 493 4134 493
Size: 3641 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6343
SphI G_CATG'C
Cuts at: 1132 3701 1132
Size: 2569 3774
SspI AAT'ATT
Cuts at: 5 52 4267 5
Size: 47 4215 2081
Fragments arranged by size:
4215 2081 47
StuI AGG'CCT
Cuts at: 1517 2255 2633 1517
Size: 738 378 5227
Fragments arranged by size:
5227 738 378
StyI C'CwwG_G
Cuts at: 513 1065 2004 2118 2628 3159 3782 513
Size: 552 939 114 510 531 623 3074
Fragments arranged by size:
3074 939 623 552 531 510 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 4370 4387 4540 4725 5170 325
Size: 1255 417 2373 17 153 185 445 1498
Fragments arranged by size:
2373 1498 1255 445 417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 4589 6301
Size: 274 80 33 51 1443 234 2378 1712
Cuts at: 6301 96
Size: 138
Fragments arranged by size:
2378 1712 1443 274 234 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6303 6335 6303
Size: 32 6311
Tth111I GACn'n_nGTC
Cuts at: 1316 2054 1316
Size: 738 5605
XbaI T'CTAG_A
Cuts at: 3679 3679
Size: 6343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2519 2561 3551 2519
Size: 42 990 5311
Fragments arranged by size:
5311 990 42
XhoI C'TCGA_G
Cuts at: 1943 2751 1943
Size: 808 5535
XmaI C'CCGG_G
Cuts at: 1870 3668 1870
Size: 1798 4545
XmnI GAAnn'nnTTC
Cuts at: 4472 4472
Size: 6343
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4151 276
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstXI BstZ17I DraIII EcoNI FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI
PvuII RsrII SacII SexAI SfiI SrfI SwaI