Restriction map for
pFN21AE5616
(Circular) (Six-base) MAPSORT of: pFN21AE5616.seq Check: 5161 from: 1 to: 6211
pFN21AE5616 6211 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3451 3451
Size: 6211
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4021 278
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3172 3554 1937
Size: 1235 382 4594
Fragments arranged by size:
4594 1235 382
Acc65I G'GTAC_C
Cuts at: 2298 3532 2298
Size: 1234 4977
AclI AA'CG_TT
Cuts at: 1563 4338 4711 1563
Size: 2775 373 3063
Fragments arranged by size:
3063 2775 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2473 3269 3304 4274 5658 5926 1632
Size: 841 796 35 970 1384 268 1917
Fragments arranged by size:
1917 1384 970 841 796 268 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6014
AflIII A'CryG_T
Cuts at: 1203 5110 6087 1203
Size: 3907 977 1327
Fragments arranged by size:
3907 1327 977
AgeI A'CCGG_T
Cuts at: 5042 5042
Size: 6211
AhdI GACnn_n'nnGTC
Cuts at: 4940 4940
Size: 6211
AleI CACnn'nnGTG
Cuts at: 1166 2700 1166
Size: 1534 4677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3158 1184
Size: 1974 4237
AlwNI CAG_nnn'CTG
Cuts at: 3215 3337 3391 3954 5526 3215
Size: 122 54 563 1572 3900
Fragments arranged by size:
3900 1572 563 122 54
ApaI G_GGCC'C
Cuts at: 2503 2503
Size: 6211
ApaLI G'TGCA_C
Cuts at: 4268 5424 4268
Size: 1156 5055
ApoI r'AATT_y
Cuts at: 1493 3520 3746 3898 6007 1493
Size: 2027 226 152 2109 1697
Fragments arranged by size:
2109 2027 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4765 160
Size: 872 3733 1606
Fragments arranged by size:
3733 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
AvaI C'yCGr_G
Cuts at: 1870 1943 3536 1870
Size: 73 1593 4545
Fragments arranged by size:
4545 1593 73
BamHI G'GATC_C
Cuts at: 3470 3541 3908 3470
Size: 71 367 5773
Fragments arranged by size:
5773 367 71
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2298 3532 4987 618
Size: 325 217 627 511 1234 1455 1842
Fragments arranged by size:
1842 1455 1234 627 511 325 217
BanII G_rGCy'C
Cuts at: 1391 2503 2973 3530 1391
Size: 1112 470 557 4072
Fragments arranged by size:
4072 1112 557 470
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2479 2571 928
Size: 971 580 92 4568
Fragments arranged by size:
4568 971 580 92
BbvCI CC'TCA_GC
Cuts at: 2268 2268
Size: 6211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4402 4436 1903
Size: 34 2465 34 3678
Fragments arranged by size:
3678 2465 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3388 4103 5313 3388
Size: 715 1210 4286
Fragments arranged by size:
4286 1210 715
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6211
BfrBI ATG'CAT
Cuts at: 2214 2214
Size: 6211
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4822 136
Size: 107 122 71 631 605 3150 1525
Fragments arranged by size:
3150 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6206 6206
Size: 6211
BlpI GC'TnA_GC
Cuts at: 2028 3341 3628 2028
Size: 1313 287 4611
Fragments arranged by size:
4611 1313 287
Bme1580I G_kGCm'C
Cuts at: 1790 2503 2947 3008 4272 5428 1790
Size: 713 444 61 1264 1156 2573
Fragments arranged by size:
2573 1264 1156 713 444 61
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6211
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3942 4900 442
Size: 416 53 83 399 24 2525 958 1753
Fragments arranged by size:
2525 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6211
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2267 2267
Size: 6211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2115 2300 2398 2802 2820 2829 3223 3463
Size: 876 185 98 404 18 9 394 240
Cuts at: 3463 4871 1239
Size: 1408 2579
Fragments arranged by size:
2579 1408 876 404 394 240 185 98
18 9
Bpu10I CC'TnA_GC
Cuts at: 2079 2268 3216 2079
Size: 189 948 5074
Fragments arranged by size:
5074 948 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2477 2621 4336 5201 5499 2477
Size: 144 1715 865 298 3189
Fragments arranged by size:
3189 1715 865 298 144
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2385 4874 882
Size: 617 886 2489 2219
Fragments arranged by size:
2489 2219 886 617
BsaAI yAC'GTr
Cuts at: 493 1204 4002 493
Size: 711 2798 2702
Fragments arranged by size:
2798 2702 711
BsaBI GATnn'nnATC
Cuts at: 6205 6205
Size: 6211
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4018 4400 5035 275
Size: 53 83 186 936 2485 382 635 1451
Fragments arranged by size:
2485 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2816 3453 3490 4644 5042 5316 5463 5874 2816
Size: 637 37 1154 398 274 147 411 3153
Fragments arranged by size:
3153 1154 637 411 398 274 147 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3407 3437 1178
Size: 30 165 30 2004 30 3952
Fragments arranged by size:
3952 2004 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6211
BseYI C'CCAG_C
Cuts at: 5414 5910 5414
Size: 496 5715
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3164 3228 3404 1163
Size: 573 1428 64 176 3970
Fragments arranged by size:
3970 1428 573 176 64
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3661 4422 5450 1540
Size: 275 186 1660 761 1028 2301
Fragments arranged by size:
2301 1660 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3037 3449 4272 4357 5428 3037
Size: 412 823 85 1071 3820
Fragments arranged by size:
3820 1071 823 412 85
BsmI GAATG_Cn'
Cuts at: 3722 3815 3722
Size: 93 6118
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2503 2947 2973 3008 3037 3449 3530
Size: 399 713 444 26 35 29 412 81
Cuts at: 3530 4272 4357 5428 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 713 444 412 399 85
81 35 29 26
BspEI T'CCGG_A
Cuts at: 2816 3453 5874 2816
Size: 637 2421 3153
Fragments arranged by size:
3153 2421 637
BspHI T'CATG_A
Cuts at: 4100 5830 4100
Size: 1730 4481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3451 3566 844
Size: 2607 115 3489
Fragments arranged by size:
3489 2607 115
BsrBI CCG'CTC
Cuts at: 1394 1823 4098 1394
Size: 429 2275 3507
Fragments arranged by size:
3507 2275 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4706 4880 65
Size: 1183 3458 174 1396
Fragments arranged by size:
3458 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2164 4855 5042 1700
Size: 110 354 2691 187 2869
Fragments arranged by size:
2869 2691 354 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6211
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6211
BssSI C'ACGA_G
Cuts at: 4271 5283 4271
Size: 1012 5199
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6211
BstBI TT'CG_AA
Cuts at: 5015 5015
Size: 6211
BstEII G'GTnAC_C
Cuts at: 1184 2626 1184
Size: 1442 4769
BstYI r'GATC_y
Cuts at: 1972 3293 3470 3541 3908 4293 4310 5762 5848
Size: 1321 177 71 367 385 17 1452 86
Cuts at: 5848 5860 6206 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1321 385 367 346 177 86
71 17 12
Bsu36I CC'TnA_GG
Cuts at: 3377 3377
Size: 6211
BtgI C'CryG_G
Cuts at: 513 1065 2013 2872 513
Size: 552 948 859 3852
Fragments arranged by size:
3852 948 859 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5472
Fragments arranged by size:
5472 683 56
BtsI GCAGTG_nn'
Cuts at: 3142 3727 4521 4541 3142
Size: 585 794 20 4812
Fragments arranged by size:
4812 794 585 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6176
DraI TTT'AAA
Cuts at: 3515 3515
Size: 6211
DraIII CAC_nnn'GTG
Cuts at: 2429 2845 2429
Size: 416 5795
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5218 809
Size: 507 3902 1802
Fragments arranged by size:
3902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3365 3658 4547
Size: 54 1425 50 275 18 1535 293 889
Cuts at: 4547 6176 8
Size: 1629 43
Fragments arranged by size:
1629 1535 1425 889 293 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3658 1537
Size: 275 1846 4090
Fragments arranged by size:
4090 1846 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2961 3323 4141 1353
Size: 1608 362 818 3423
Fragments arranged by size:
3423 1608 818 362
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4798 5172 5318 387
Size: 1064 138 3209 374 146 1280
Fragments arranged by size:
3209 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2300 2398 2473 2802 2820 2829
Size: 393 483 185 98 75 329 18 9
Cuts at: 2829 3223 3269 3304 3463 4274 4871 5658 5926
Size: 394 46 35 159 811 597 787 268
Cuts at: 5926 1239
Size: 1524
Fragments arranged by size:
1524 811 787 597 483 394 393 329
268 185 159 98 75 46 35 18
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2336 2336
Size: 6211
EcoRI G'AATT_C
Cuts at: 3520 3898 3520
Size: 378 5833
FspI TGC'GCA
Cuts at: 4717 4717
Size: 6211
HaeII r_GCGC'y
Cuts at: 1678 5358 1678
Size: 3680 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 2379 2411 2910
Size: 32 135 32 541 32 234 32 499
Cuts at: 2910 2942 3285 3317 3407 3439 3462 3494 4852
Size: 32 343 32 90 32 23 32 1358
Cuts at: 4852 4884 4926 4958 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1358 541 499 343 234 135 90
42 32 32 32 32 32 32 32
32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 3173 3555 3801 669
Size: 988 281 1235 382 246 3079
Fragments arranged by size:
3079 1235 988 382 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6211
HpaI GTT'AAC
Cuts at: 3801 3801
Size: 6211
KpnI G_GTAC'C
Cuts at: 2302 3536 2302
Size: 1234 4977
MfeI C'AATT_G
Cuts at: 3273 3810 3273
Size: 537 5674
MluI A'CGCG_T
Cuts at: 6087 6087
Size: 6211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5325 5509 1305
Size: 4020 184 2007
Fragments arranged by size:
4020 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3367 10
Size: 54 1425 343 1535 2854
Fragments arranged by size:
2854 1535 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 2450 2700 4169 4528 4687 518
Size: 648 1236 48 250 1469 359 159 2042
Fragments arranged by size:
2042 1469 1236 648 359 250 159 48
MspA1I CmG'CkG
Cuts at: 1606 2015 2111 2135 2700 2987 3201 3627 4304
Size: 409 96 24 565 287 214 426 677
Cuts at: 4304 5452 5697 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 565 426 409 287 245
214 96 24
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5659
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6211
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6211
NotI GC'GGCC_GC
Cuts at: 3658 3658
Size: 6211
NsiI A_TGCA'T
Cuts at: 2216 2216
Size: 6211
NspI r_CATG'y
Cuts at: 1132 1229 3569 5114 1132
Size: 97 2340 1545 2229
Fragments arranged by size:
2340 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6211
PciI A'CATG_T
Cuts at: 5110 5110
Size: 6211
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2873 3201 1108
Size: 1765 328 4118
Fragments arranged by size:
4118 1765 328
PfoI T'CCnGG_A
Cuts at: 2542 3473 2542
Size: 931 5280
PmeI GTTT'AAAC
Cuts at: 3515 3515
Size: 6211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 5793 5825 4280
Size: 32 1481 32 4666
Fragments arranged by size:
4666 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6211
PsiI TTA'TAA
Cuts at: 3781 3781
Size: 6211
PspOMI G'GGCC_C
Cuts at: 2499 2499
Size: 6211
PstI C_TGCA'G
Cuts at: 830 2617 3108 3563 830
Size: 1787 491 455 3478
Fragments arranged by size:
3478 1787 491 455
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6211
PvuII CAG'CTG
Cuts at: 2135 2700 2987 3201 2135
Size: 565 287 214 5145
Fragments arranged by size:
5145 565 287 214
SacII CC_GC'GG
Cuts at: 2016 2016
Size: 6211
SalI G'TCGA_C
Cuts at: 1936 3171 3553 1936
Size: 1235 382 4594
Fragments arranged by size:
4594 1235 382
SapI GCTCTTCn'nnn_
Cuts at: 2961 2961
Size: 6211
SbfI CC_TGCA'GG
Cuts at: 3563 3563
Size: 6211
ScaI AGT'ACT
Cuts at: 4459 6171 4459
Size: 1712 4499
SexAI A'CCwGG_T
Cuts at: 2841 2841
Size: 6211
SfcI C'TryA_G
Cuts at: 826 1045 2613 2712 2899 3104 3559 4694 5375
Size: 219 1568 99 187 205 455 1135 681
Cuts at: 5375 5566 826
Size: 191 1471
Fragments arranged by size:
1568 1471 1135 681 455 219 205 191
187 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6211
SmaI CCC'GGG
Cuts at: 1872 3538 1872
Size: 1666 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2456 2600 4315 5216 5478 820
Size: 197 926 513 144 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 513 262 197 144
SnaBI TAC'GTA
Cuts at: 493 4002 493
Size: 3509 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6211
SphI G_CATG'C
Cuts at: 1132 3569 1132
Size: 2437 3774
SspI AAT'ATT
Cuts at: 5 52 4135 5
Size: 47 4083 2081
Fragments arranged by size:
4083 2081 47
StuI AGG'CCT
Cuts at: 1517 2144 1517
Size: 627 5584
StyI C'CwwG_G
Cuts at: 513 1065 2938 3650 513
Size: 552 1873 712 3074
Fragments arranged by size:
3074 1873 712 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4238 4255 4408 4593 5038 325
Size: 1255 2658 17 153 185 445 1498
Fragments arranged by size:
2658 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3408 4457 6169
Size: 274 80 33 51 1443 1431 1049 1712
Cuts at: 6169 96
Size: 138
Fragments arranged by size:
1712 1443 1431 1049 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2676 2708 6171 6203 2676
Size: 32 3463 32 2684
Fragments arranged by size:
3463 2684 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6211
XbaI T'CTAG_A
Cuts at: 3547 3547
Size: 6211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2091 2426 2530 2654 2091
Size: 335 104 124 5648
Fragments arranged by size:
5648 335 124 104
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6211
XmaI C'CCGG_G
Cuts at: 1870 3536 1870
Size: 1666 4545
XmnI GAAnn'nnTTC
Cuts at: 4340 4340
Size: 6211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4019 276
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SanDI SfiI SfoI SrfI
SwaI