Restriction map for
pFN21AE5615
(Circular) (Six-base) MAPSORT of: pFN21AE5615.seq Check: 2407 from: 1 to: 6418
pFN21AE5615 6418 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4228 278
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 3228 3761 1937
Size: 894 397 533 4594
Fragments arranged by size:
4594 894 533 397
Acc65I G'GTAC_C
Cuts at: 2211 3739 2211
Size: 1528 4890
AclI AA'CG_TT
Cuts at: 1563 4545 4918 1563
Size: 2982 373 3063
Fragments arranged by size:
3063 2982 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2883 4481 5865 6133 1632
Size: 1251 1598 1384 268 1917
Fragments arranged by size:
1917 1598 1384 1251 268
AflII C'TTAA_G
Cuts at: 820 1017 3437 820
Size: 197 2420 3801
Fragments arranged by size:
3801 2420 197
AflIII A'CryG_T
Cuts at: 1203 5317 6294 1203
Size: 4114 977 1327
Fragments arranged by size:
4114 1327 977
AgeI A'CCGG_T
Cuts at: 5249 5249
Size: 6418
AhdI GACnn_n'nnGTC
Cuts at: 5147 5147
Size: 6418
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3723 1184
Size: 2539 3879
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2895 2927 2895
Size: 32 6386
AlwNI CAG_nnn'CTG
Cuts at: 4161 5733 4161
Size: 1572 4846
ApaLI G'TGCA_C
Cuts at: 2612 4475 5631 2612
Size: 1863 1156 3399
Fragments arranged by size:
3399 1863 1156
ApoI r'AATT_y
Cuts at: 1493 2079 2201 2426 2891 3260 3278 3335 3727
Size: 586 122 225 465 369 18 57 392
Cuts at: 3727 3953 4105 6214 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 586 465 392 369 226 225
152 122 57 18
AseI AT'TA_AT
Cuts at: 160 1032 2694 4972 160
Size: 872 1662 2278 1606
Fragments arranged by size:
2278 1662 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
AvaI C'yCGr_G
Cuts at: 1870 1943 2304 3743 1870
Size: 73 361 1439 4545
Fragments arranged by size:
4545 1439 361 73
BamHI G'GATC_C
Cuts at: 3748 4115 3748
Size: 367 6051
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2211 3739 5194 618
Size: 325 217 627 424 1528 1455 1842
Fragments arranged by size:
1842 1528 1455 627 424 325 217
BanII G_rGCy'C
Cuts at: 1391 3737 1391
Size: 2346 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2595 3000 928
Size: 971 696 405 4346
Fragments arranged by size:
4346 971 696 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2915 2949 4609 4643 1903
Size: 34 978 34 1660 34 3678
Fragments arranged by size:
3678 1660 978 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4310 5520 4310
Size: 1210 5208
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6418
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5029 136
Size: 107 122 71 631 605 3357 1525
Fragments arranged by size:
3357 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6413 6413
Size: 6418
BlpI GC'TnA_GC
Cuts at: 3835 3835
Size: 6418
Bme1580I G_kGCm'C
Cuts at: 1790 2616 4479 5635 1790
Size: 826 1863 1156 2573
Fragments arranged by size:
2573 1863 1156 826
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6418
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4149 5107 442
Size: 416 53 83 399 24 2732 958 1753
Fragments arranged by size:
2732 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6418
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3590
Size: 6418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2046 2424 3168 5078 1239
Size: 807 378 744 1910 2579
Fragments arranged by size:
2579 1910 807 744 378
Bpu10I CC'TnA_GC
Cuts at: 3631 3631
Size: 6418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4543 5408 5706 4543
Size: 865 298 5255
Fragments arranged by size:
5255 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2307 3197 5081 882
Size: 617 808 890 1884 2219
Fragments arranged by size:
2219 1884 890 808 617
BsaAI yAC'GTr
Cuts at: 493 1204 4209 493
Size: 711 3005 2702
Fragments arranged by size:
3005 2702 711
BsaBI GATnn'nnATC
Cuts at: 2018 6412 2018
Size: 4394 2024
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4225 4607 5242 275
Size: 53 83 186 936 2692 382 635 1451
Fragments arranged by size:
2692 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4851 5249 5523 5670 6081 4851
Size: 398 274 147 411 5188
Fragments arranged by size:
5188 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3539 3569 1178
Size: 30 165 30 2136 30 4027
Fragments arranged by size:
4027 2136 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6418
BseYI C'CCAG_C
Cuts at: 5621 6117 5621
Size: 496 5922
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2521 3274 3678 1163
Size: 573 785 753 404 3903
Fragments arranged by size:
3903 785 753 573 404
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3868 4629 5657 1540
Size: 275 186 1867 761 1028 2301
Fragments arranged by size:
2301 1867 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2616 4479 4564 5635 2616
Size: 1863 85 1071 3399
Fragments arranged by size:
3399 1863 1071 85
BsiWI C'GTAC_G
Cuts at: 2844 2844
Size: 6418
BsmI GAATG_Cn'
Cuts at: 2808 3929 4022 2808
Size: 1121 93 5204
Fragments arranged by size:
5204 1121 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2616 3737 4479 4564 5635 1391
Size: 399 826 1121 742 85 1071 2174
Fragments arranged by size:
2174 1121 1071 826 742 399 85
BspEI T'CCGG_A
Cuts at: 6081 6081
Size: 6418
BspHI T'CATG_A
Cuts at: 2400 3186 4307 6037 2400
Size: 786 1121 1730 2781
Fragments arranged by size:
2781 1730 1121 786
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3773 844
Size: 2929 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4305 1394
Size: 429 2482 3507
Fragments arranged by size:
3507 2482 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2977 3707 4913 5087 65
Size: 1183 1729 730 1206 174 1396
Fragments arranged by size:
1729 1396 1206 1183 730 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5062 5249 1700
Size: 110 3252 187 2869
Fragments arranged by size:
3252 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6418
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6418
BssSI C'ACGA_G
Cuts at: 2220 4478 5490 2220
Size: 2258 1012 3148
Fragments arranged by size:
3148 2258 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6418
BstBI TT'CG_AA
Cuts at: 5222 5222
Size: 6418
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6418
BstXI CCAn_nnnn'nTGG
Cuts at: 3119 3119
Size: 6418
BstYI r'GATC_y
Cuts at: 1972 3748 4115 4500 4517 5969 6055 6067 6413
Size: 1776 367 385 17 1452 86 12 346
Cuts at: 6413 1972
Size: 1977
Fragments arranged by size:
1977 1776 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2498 513
Size: 552 939 494 4433
Fragments arranged by size:
4433 939 552 494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5679
Fragments arranged by size:
5679 683 56
BtsI GCAGTG_nn'
Cuts at: 2302 3934 4728 4748 2302
Size: 1632 794 20 3972
Fragments arranged by size:
3972 1632 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2938 2973 529
Size: 35 2374 35 3974
Fragments arranged by size:
3974 2374 35 35
DraI TTT'AAA
Cuts at: 3722 3722
Size: 6418
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5425 809
Size: 507 4109 1802
Fragments arranged by size:
4109 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2324 2946 3865
Size: 54 1425 50 275 18 494 622 919
Cuts at: 3865 4754 6383 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 919 889 622 494 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3865 1537
Size: 275 2053 4090
Fragments arranged by size:
4090 2053 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4348 1353
Size: 2995 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5005 5379 5525 387
Size: 1064 138 3416 374 146 1280
Fragments arranged by size:
3416 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2424 2883 3168 4481 5078 5865
Size: 393 414 378 459 285 1313 597 787
Cuts at: 5865 6133 1239
Size: 268 1524
Fragments arranged by size:
1524 1313 787 597 459 414 393 378
285 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3630 3630
Size: 6418
EcoO109I rG'GnC_Cy
Cuts at: 3582 3582
Size: 6418
EcoRI G'AATT_C
Cuts at: 2079 3260 3278 3727 4105 2079
Size: 1181 18 449 378 4392
Fragments arranged by size:
4392 1181 449 378 18
EcoRV GAT'ATC
Cuts at: 2362 2605 2362
Size: 243 6175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3544 3544
Size: 6418
FspI TGC'GCA
Cuts at: 4924 4924
Size: 6418
HaeII r_GCGC'y
Cuts at: 1678 5565 1678
Size: 3887 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3159 3191 5059 5091 5133
Size: 32 135 32 1587 32 1868 32 42
Cuts at: 5133 5165 1373
Size: 32 2626
Fragments arranged by size:
2626 1868 1587 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2755 2832 3505 3762 4008 669
Size: 988 281 817 77 673 257 246 3079
Fragments arranged by size:
3079 988 817 673 281 257 246 77
HindIII A'AGCT_T
Cuts at: 748 3514 748
Size: 2766 3652
HpaI GTT'AAC
Cuts at: 2755 4008 2755
Size: 1253 5165
KpnI G_GTAC'C
Cuts at: 2215 3743 2215
Size: 1528 4890
MfeI C'AATT_G
Cuts at: 4017 4017
Size: 6418
MluI A'CGCG_T
Cuts at: 6294 6294
Size: 6418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2337 5532 5716 1305
Size: 1032 3195 184 2007
Fragments arranged by size:
3195 2007 1032 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2326 2948 10
Size: 54 1425 343 494 622 3480
Fragments arranged by size:
3480 1425 622 494 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4376 4735 4894 518
Size: 648 3210 359 159 2042
Fragments arranged by size:
3210 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2804 3834 4511 5659 5904 1606
Size: 1198 1030 677 1148 245 2120
Fragments arranged by size:
2120 1198 1148 1030 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4927
Fragments arranged by size:
4927 939 552
NdeI CA'TA_TG
Cuts at: 387 3679 387
Size: 3292 3126
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6418
NotI GC'GGCC_GC
Cuts at: 3865 3865
Size: 6418
NspI r_CATG'y
Cuts at: 1132 1229 2495 2930 3776 5321 1132
Size: 97 1266 435 846 1545 2229
Fragments arranged by size:
2229 1545 1266 846 435 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6418
PciI A'CATG_T
Cuts at: 5317 5317
Size: 6418
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2107 3680 1108
Size: 999 1573 3846
Fragments arranged by size:
3846 1573 999
PfoI T'CCnGG_A
Cuts at: 2761 2761
Size: 6418
PmeI GTTT'AAAC
Cuts at: 3722 3722
Size: 6418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4487 4519 6000 6032 4487
Size: 32 1481 32 4873
Fragments arranged by size:
4873 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3582 3582
Size: 6418
PshAI GACnn'nnGTC
Cuts at: 1613 3162 1613
Size: 1549 4869
PsiI TTA'TAA
Cuts at: 3988 3988
Size: 6418
PstI C_TGCA'G
Cuts at: 830 2488 3770 830
Size: 1658 1282 3478
Fragments arranged by size:
3478 1658 1282
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6418
SalI G'TCGA_C
Cuts at: 1936 2830 3760 1936
Size: 894 930 4594
Fragments arranged by size:
4594 930 894
SbfI CC_TGCA'GG
Cuts at: 3770 3770
Size: 6418
ScaI AGT'ACT
Cuts at: 4666 6378 4666
Size: 1712 4706
SfcI C'TryA_G
Cuts at: 826 1045 2241 2484 3577 3766 4901 5582 5773
Size: 219 1196 243 1093 189 1135 681 191
Cuts at: 5773 826
Size: 1471
Fragments arranged by size:
1471 1196 1135 1093 681 243 219 191
189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6418
SmaI CCC'GGG
Cuts at: 1872 3745 1872
Size: 1873 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2304 3437 4522 5423 5685 820
Size: 197 926 361 1133 1085 901 262 1553
Fragments arranged by size:
1553 1133 1085 926 901 361 262 197
SnaBI TAC'GTA
Cuts at: 493 4209 493
Size: 3716 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6418
SphI G_CATG'C
Cuts at: 1132 3776 1132
Size: 2644 3774
SspI AAT'ATT
Cuts at: 5 52 3003 4342 5
Size: 47 2951 1339 2081
Fragments arranged by size:
2951 2081 1339 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6418
StyI C'CwwG_G
Cuts at: 513 1065 2004 2327 2704 3562 3857 513
Size: 552 939 323 377 858 295 3074
Fragments arranged by size:
3074 939 858 552 377 323 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 3414 4445 4462 4615 4800 5245
Size: 1255 1035 799 1031 17 153 185 445
Cuts at: 5245 325
Size: 1498
Fragments arranged by size:
1498 1255 1035 1031 799 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4664 6376 96
Size: 274 80 33 51 1443 2687 1712 138
Fragments arranged by size:
2687 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3539 3571 6378 6410 3539
Size: 32 2807 32 3547
Fragments arranged by size:
3547 2807 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6418
XbaI T'CTAG_A
Cuts at: 3754 3754
Size: 6418
XhoI C'TCGA_G
Cuts at: 1943 2304 1943
Size: 361 6057
XmaI C'CCGG_G
Cuts at: 1870 3743 1870
Size: 1873 4545
XmnI GAAnn'nnTTC
Cuts at: 2592 4547 2592
Size: 1955 4463
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4226 276
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI