Restriction map for
pFN21AE5612
(Circular) (Six-base) MAPSORT of: pFN21AE5612.seq Check: 9857 from: 1 to: 6238
pFN21AE5612 6238 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2415 2972 3581 1937
Size: 478 557 609 4594
Fragments arranged by size:
4594 609 557 478
Acc65I G'GTAC_C
Cuts at: 3559 3559
Size: 6238
AclI AA'CG_TT
Cuts at: 1563 4365 4738 1563
Size: 2802 373 3063
Fragments arranged by size:
3063 2802 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4301 5685 5953 1632
Size: 2669 1384 268 1917
Fragments arranged by size:
2669 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 5137 6114 1203
Size: 3934 977 1327
Fragments arranged by size:
3934 1327 977
AgeI A'CCGG_T
Cuts at: 5069 5069
Size: 6238
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6238
AlwNI CAG_nnn'CTG
Cuts at: 3981 5553 3981
Size: 1572 4666
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 2018 2113 2177 2192 2576 2945 3181 3299
Size: 525 95 64 15 384 369 236 118
Cuts at: 3299 3441 3547 3773 3925 6034 1493
Size: 142 106 226 152 2109 1697
Fragments arranged by size:
2109 1697 525 384 369 236 226 152
142 118 106 95 64 15
AseI AT'TA_AT
Cuts at: 160 1032 4792 160
Size: 872 3760 1606
Fragments arranged by size:
3760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 3563 1870
Size: 73 1620 4545
Fragments arranged by size:
4545 1620 73
BamHI G'GATC_C
Cuts at: 3568 3935 3568
Size: 367 5871
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3559 5014 618
Size: 325 217 627 1772 1455 1842
Fragments arranged by size:
1842 1772 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3557 1391
Size: 2166 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3282 928
Size: 971 1383 3884
Fragments arranged by size:
3884 1383 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2972 3006 4429 4463 1903
Size: 34 1035 34 1423 34 3678
Fragments arranged by size:
3678 1423 1035 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2074 4130 5340 2074
Size: 2056 1210 2972
Fragments arranged by size:
2972 2056 1210
BclI T'GATC_A
Cuts at: 1548 2932 1548
Size: 1384 4854
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4849 136
Size: 107 122 71 631 605 3177 1525
Fragments arranged by size:
3177 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6233 6233
Size: 6238
BlpI GC'TnA_GC
Cuts at: 3655 3655
Size: 6238
Bme1580I G_kGCm'C
Cuts at: 1790 4299 5455 1790
Size: 2509 1156 2573
Fragments arranged by size:
2573 2509 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2452 3969 4927
Size: 416 53 83 399 24 1035 1517 958
Cuts at: 4927 442
Size: 1753
Fragments arranged by size:
1753 1517 1035 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3302 4898 1239
Size: 2063 1596 2579
Fragments arranged by size:
2579 2063 1596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2940 3251 3346 4363 5228 5526 2940
Size: 311 95 1017 865 298 3652
Fragments arranged by size:
3652 1017 865 311 298 95
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2449 4901 882
Size: 617 950 2452 2219
Fragments arranged by size:
2452 2219 950 617
BsaAI yAC'GTr
Cuts at: 493 1204 3257 4029 493
Size: 711 2053 772 2702
Fragments arranged by size:
2702 2053 772 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4045 4427 5062 275
Size: 53 83 186 936 2512 382 635 1451
Fragments arranged by size:
2512 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2668 2899 4671 5069 5343 5490 5901 2668
Size: 231 1772 398 274 147 411 3005
Fragments arranged by size:
3005 1772 411 398 274 231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2316 2346 1178
Size: 30 165 30 913 30 5070
Fragments arranged by size:
5070 913 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6238
BseYI C'CCAG_C
Cuts at: 5441 5937 5441
Size: 496 5742
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5665
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3688 4449 5477 1540
Size: 275 186 1687 761 1028 2301
Fragments arranged by size:
2301 1687 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2125 4299 4384 5455 2125
Size: 2174 85 1071 2908
Fragments arranged by size:
2908 2174 1071 85
BsmI GAATG_Cn'
Cuts at: 2170 3749 3842 2170
Size: 1579 93 4566
Fragments arranged by size:
4566 1579 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2125 3557 4299 4384 5455 1391
Size: 399 335 1432 742 85 1071 2174
Fragments arranged by size:
2174 1432 1071 742 399 335 85
BspEI T'CCGG_A
Cuts at: 2668 5901 2668
Size: 3233 3005
BspHI T'CATG_A
Cuts at: 4127 5857 4127
Size: 1730 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3593 844
Size: 2749 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4125 1394
Size: 429 2302 3507
Fragments arranged by size:
3507 2302 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4733 4907 65
Size: 1183 3485 174 1396
Fragments arranged by size:
3485 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4882 5069 1700
Size: 110 3072 187 2869
Fragments arranged by size:
3072 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2533 96
Size: 2437 3801
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 4298 5310 4298
Size: 1012 5226
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6238
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6238
BstYI r'GATC_y
Cuts at: 1972 3568 3935 4320 4337 5789 5875 5887 6233
Size: 1596 367 385 17 1452 86 12 346
Cuts at: 6233 1972
Size: 1977
Fragments arranged by size:
1977 1596 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2973 2973
Size: 6238
BtgI C'CryG_G
Cuts at: 513 1065 2004 3420 513
Size: 552 939 1416 3331
Fragments arranged by size:
3331 1416 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5499
Fragments arranged by size:
5499 683 56
BtsI GCAGTG_nn'
Cuts at: 2772 3754 4548 4568 2772
Size: 982 794 20 4442
Fragments arranged by size:
4442 982 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 3542 3542
Size: 6238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5245 809
Size: 507 3929 1802
Fragments arranged by size:
3929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2636 3685 4574
Size: 54 1425 50 275 18 806 1049 889
Cuts at: 4574 6203 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1049 889 806 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 4168 1353
Size: 872 1943 3423
Fragments arranged by size:
3423 1943 872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4825 5199 5345 387
Size: 1064 138 3236 374 146 1280
Fragments arranged by size:
3236 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3302 4301 4898 5685 5953 1239
Size: 393 1670 999 597 787 268 1524
Fragments arranged by size:
1670 1524 999 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3181 3547 3925 3181
Size: 366 378 5494
Fragments arranged by size:
5494 378 366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2377 2981 2047
Size: 330 604 5304
Fragments arranged by size:
5304 604 330
FspI TGC'GCA
Cuts at: 2840 4744 2840
Size: 1904 4334
HaeII r_GCGC'y
Cuts at: 1678 5385 1678
Size: 3707 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4879 4911 4953 4985 1373
Size: 32 135 32 3307 32 42 32 2626
Fragments arranged by size:
3307 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3582 3828 669
Size: 988 281 1644 246 3079
Fragments arranged by size:
3079 1644 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2041 748
Size: 1293 4945
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KpnI G_GTAC'C
Cuts at: 3563 3563
Size: 6238
MfeI C'AATT_G
Cuts at: 3837 3837
Size: 6238
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5352 5536 1305
Size: 4047 184 2007
Fragments arranged by size:
4047 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4416
Fragments arranged by size:
4416 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4196 4555 4714 518
Size: 648 843 2187 359 159 2042
Fragments arranged by size:
2187 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2754 3312 3654 4331 5479 5724 1606
Size: 1148 558 342 677 1148 245 2120
Fragments arranged by size:
2120 1148 1148 677 558 342 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3420 513
Size: 552 939 1416 3331
Fragments arranged by size:
3331 1416 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6238
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 3596 5141 1132
Size: 97 2367 1545 2229
Fragments arranged by size:
2367 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6238
PciI A'CATG_T
Cuts at: 5137 5137
Size: 6238
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6238
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6238
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PstI C_TGCA'G
Cuts at: 830 3590 830
Size: 2760 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
PvuII CAG'CTG
Cuts at: 2754 3312 2754
Size: 558 5680
SalI G'TCGA_C
Cuts at: 1936 3580 1936
Size: 1644 4594
SbfI CC_TGCA'GG
Cuts at: 3590 3590
Size: 6238
ScaI AGT'ACT
Cuts at: 4486 6198 4486
Size: 1712 4526
SfcI C'TryA_G
Cuts at: 826 1045 2817 3586 4721 5402 5593 826
Size: 219 1772 769 1135 681 191 1471
Fragments arranged by size:
1772 1471 1135 769 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6238
SmaI CCC'GGG
Cuts at: 1872 3565 1872
Size: 1693 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2955 3230 3325 4342 5243 5505
Size: 197 926 1012 275 95 1017 901 262
Cuts at: 5505 820
Size: 1553
Fragments arranged by size:
1553 1017 1012 926 901 275 262 197
95
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6238
SphI G_CATG'C
Cuts at: 1132 3596 1132
Size: 2464 3774
SspI AAT'ATT
Cuts at: 5 52 2274 4162 5
Size: 47 2222 1888 2081
Fragments arranged by size:
2222 2081 1888 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6238
StyI C'CwwG_G
Cuts at: 513 1065 2004 3420 3677 513
Size: 552 939 1416 257 3074
Fragments arranged by size:
3074 1416 939 552 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2987 4265 4282 4435 4620 5065 325
Size: 1255 1407 1278 17 153 185 445 1498
Fragments arranged by size:
1498 1407 1278 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2533 4484 6196
Size: 274 80 33 51 1443 556 1951 1712
Cuts at: 6196 96
Size: 138
Fragments arranged by size:
1951 1712 1443 556 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2316 2348 3172 3204 6198 6230 2316
Size: 32 824 32 2994 32 2324
Fragments arranged by size:
2994 2324 824 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6238
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2646 2646
Size: 6238
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6238
XmaI C'CCGG_G
Cuts at: 1870 3563 1870
Size: 1693 4545
XmnI GAAnn'nnTTC
Cuts at: 2517 2597 4367 2517
Size: 80 1770 4388
Fragments arranged by size:
4388 1770 80
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI