Restriction map for
pFN21AE5611
(Circular) (Six-base) MAPSORT of: pFN21AE5611.seq Check: 3465 from: 1 to: 6181
pFN21AE5611 6181 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2747 2747
Size: 6181
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2619 3991 278
Size: 53 83 186 936 1083 1372 2468
Fragments arranged by size:
2468 1372 1083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2943 3524 1937
Size: 1006 581 4594
Fragments arranged by size:
4594 1006 581
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 4308 4681 1563
Size: 2745 373 3063
Fragments arranged by size:
3063 2745 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4244 5628 5896 1632
Size: 2612 1384 268 1917
Fragments arranged by size:
2612 1917 1384 268
AfeI AGC'GCT
Cuts at: 2105 2144 2171 2105
Size: 39 27 6115
Fragments arranged by size:
6115 39 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 2244 5080 6057 1203
Size: 1041 2836 977 1327
Fragments arranged by size:
2836 1327 1041 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 4910 4910
Size: 6181
AleI CACnn'nnGTG
Cuts at: 1166 2997 1166
Size: 1831 4350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6181
AlwNI CAG_nnn'CTG
Cuts at: 2122 2193 2407 2749 3042 3201 3924 5496 2122
Size: 71 214 342 293 159 723 1572 2807
Fragments arranged by size:
2807 1572 723 342 293 214 159 71
ApaI G_GGCC'C
Cuts at: 2026 2074 2083 2214 2458 3468 2026
Size: 48 9 131 244 1010 4739
Fragments arranged by size:
4739 1010 244 131 48 9
ApaLI G'TGCA_C
Cuts at: 4238 5394 4238
Size: 1156 5025
ApoI r'AATT_y
Cuts at: 1493 3490 3716 3868 5977 1493
Size: 1997 226 152 2109 1697
Fragments arranged by size:
2109 1997 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4735 160
Size: 872 3703 1606
Fragments arranged by size:
3703 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2266 2450 2953 3506 1870
Size: 73 102 221 184 503 553 4545
Fragments arranged by size:
4545 553 503 221 184 102 73
BamHI G'GATC_C
Cuts at: 3511 3878 3511
Size: 367 5814
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2735 3020 3502 4957 618
Size: 325 217 627 948 285 482 1455 1842
Fragments arranged by size:
1842 1455 948 627 482 325 285 217
BanII G_rGCy'C
Cuts at: 1391 2012 2026 2074 2083 2214 2305 2458 2960
Size: 621 14 48 9 131 91 153 502
Cuts at: 2960 3135 3468 3500 1391
Size: 175 333 32 4072
Fragments arranged by size:
4072 621 502 333 175 153 131 91
48 32 14 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2305 928
Size: 971 406 4804
Fragments arranged by size:
4804 971 406
BbvCI CC'TCA_GC
Cuts at: 2166 2166
Size: 6181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4372 4406 1903
Size: 34 2435 34 3678
Fragments arranged by size:
3678 2435 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2538 4073 5283 2538
Size: 1535 1210 3436
Fragments arranged by size:
3436 1535 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 3184 4792
Size: 107 122 71 631 605 406 1106 1608
Cuts at: 4792 136
Size: 1525
Fragments arranged by size:
1608 1525 1106 631 605 406 122 107
71
BglII A'GATC_T
Cuts at: 6176 6176
Size: 6181
BlpI GC'TnA_GC
Cuts at: 3598 3598
Size: 6181
Bme1580I G_kGCm'C
Cuts at: 1790 2026 2074 2083 2214 2379 2458 3468 4242
Size: 236 48 9 131 165 79 1010 774
Cuts at: 4242 5398 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1010 774 236 165 131 79
48 9
BmgBI CAC'GTC
Cuts at: 1318 2245 1318
Size: 927 5254
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2238 3912 4870
Size: 416 53 83 399 24 821 1674 958
Cuts at: 4870 442
Size: 1753
Fragments arranged by size:
1753 1674 958 821 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 3416 4841 1239
Size: 806 1371 1425 2579
Fragments arranged by size:
2579 1425 1371 806
Bpu10I CC'TnA_GC
Cuts at: 2166 2166
Size: 6181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4306 5171 5469 4306
Size: 865 298 5018
Fragments arranged by size:
5018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4844 882
Size: 617 3345 2219
Fragments arranged by size:
3345 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3972 493
Size: 711 2768 2702
Fragments arranged by size:
2768 2702 711
BsaBI GATnn'nnATC
Cuts at: 6175 6175
Size: 6181
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2616 3033 3988 4370
Size: 53 83 186 936 1083 417 955 382
Cuts at: 4370 5005 275
Size: 635 1451
Fragments arranged by size:
1451 1083 955 936 635 417 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4614 5012 5286 5433 5844 4614
Size: 398 274 147 411 4951
Fragments arranged by size:
4951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5956
Fragments arranged by size:
5956 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6181
BseYI C'CCAG_C
Cuts at: 2724 2772 5384 5880 2724
Size: 48 2612 496 3025
Fragments arranged by size:
3025 2612 496 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3043 1163
Size: 573 1307 4301
Fragments arranged by size:
4301 1307 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2292 3631 4392 5420 1540
Size: 275 186 291 1339 761 1028 2301
Fragments arranged by size:
2301 1339 1028 761 291 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2929 2967 3135 4242 4327 5398 2325
Size: 604 38 168 1107 85 1071 3108
Fragments arranged by size:
3108 1107 1071 604 168 85 38
BsmI GAATG_Cn'
Cuts at: 2800 3692 3785 2800
Size: 892 93 5196
Fragments arranged by size:
5196 892 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2026 2074 2083 2214 2305 2325
Size: 399 222 14 48 9 131 91 20
Cuts at: 2325 2379 2458 2929 2960 2967 3135 3468 3500
Size: 54 79 471 31 7 168 333 32
Cuts at: 3500 4242 4327 5398 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 471 399 333 222 168
131 91 85 79 54 48 32 31
20 14 9 7
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 4070 5800 4070
Size: 1730 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2747 3123 3536 844
Size: 1903 376 413 3489
Fragments arranged by size:
3489 1903 413 376
BsrBI CCG'CTC
Cuts at: 1394 1823 2015 2296 4068 1394
Size: 429 192 281 1772 3507
Fragments arranged by size:
3507 1772 429 281 192
BsrDI GCAATG_nn'
Cuts at: 65 1248 4676 4850 65
Size: 1183 3428 174 1396
Fragments arranged by size:
3428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2982 3023 4825 5012 1700
Size: 110 1172 41 1802 187 2869
Fragments arranged by size:
2869 1802 1172 187 110 41
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6181
BssHII G'CGCG_C
Cuts at: 1925 2433 2562 1925
Size: 508 129 5544
Fragments arranged by size:
5544 508 129
BssSI C'ACGA_G
Cuts at: 4241 5253 4241
Size: 1012 5169
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6181
BstBI TT'CG_AA
Cuts at: 4985 4985
Size: 6181
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6181
BstYI r'GATC_y
Cuts at: 1972 3364 3511 3878 4263 4280 5732 5818 5830
Size: 1392 147 367 385 17 1452 86 12
Cuts at: 5830 6176 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1392 385 367 346 147 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2516 2516
Size: 6181
BtgI C'CryG_G
Cuts at: 513 1065 2004 3375 513
Size: 552 939 1371 3319
Fragments arranged by size:
3319 1371 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2458 3289 487
Size: 683 56 1232 831 3379
Fragments arranged by size:
3379 1232 831 683 56
BtsI GCAGTG_nn'
Cuts at: 2965 3697 4491 4511 2965
Size: 732 794 20 4635
Fragments arranged by size:
4635 794 732 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6146
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DraIII CAC_nnn'GTG
Cuts at: 2539 2539
Size: 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5188 809
Size: 507 3872 1802
Fragments arranged by size:
3872 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2289 2409 2556
Size: 54 1425 50 275 18 459 120 147
Cuts at: 2556 2811 3628 4517 6146 8
Size: 255 817 889 1629 43
Fragments arranged by size:
1629 1425 889 817 459 275 255 147
120 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2289 3628 1537
Size: 275 477 1339 4090
Fragments arranged by size:
4090 1339 477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2783 4111 1353
Size: 1430 1328 3423
Fragments arranged by size:
3423 1430 1328
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2639 3125 4768 5142 5288 387
Size: 1064 138 1050 486 1643 374 146 1280
Fragments arranged by size:
1643 1280 1064 1050 486 374 146 138
EcoICRI GAG'CTC
Cuts at: 3133 3133
Size: 6181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 3416 4244 4841 5628 5896 1239
Size: 393 413 1371 828 597 787 268 1524
Fragments arranged by size:
1524 1371 828 787 597 413 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2515 2515
Size: 6181
EcoO109I rG'GnC_Cy
Cuts at: 2023 2070 2079 2080 2210 2404 2454 2764 2849
Size: 47 9 1 130 194 50 310 85
Cuts at: 2849 3039 3464 2023
Size: 190 425 4740
Fragments arranged by size:
4740 425 310 194 190 130 85 50
47 9 1
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
FspI TGC'GCA
Cuts at: 2358 4687 2358
Size: 2329 3852
HaeII r_GCGC'y
Cuts at: 1678 2045 2107 2146 2173 2552 5328 1678
Size: 367 62 39 27 379 2776 2531
Fragments arranged by size:
2776 2531 379 367 62 39 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2149 2181 2433 2465 2512
Size: 32 135 32 577 32 252 32 47
Cuts at: 2512 2544 3462 3494 4822 4854 4896 4928 1373
Size: 32 918 32 1328 32 42 32 2626
Fragments arranged by size:
2626 1328 918 577 252 135 47 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3525 3771 669
Size: 988 281 1587 246 3079
Fragments arranged by size:
3079 1587 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6181
HpaI GTT'AAC
Cuts at: 3771 3771
Size: 6181
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 2622 2701 3267 5295 5479 1305
Size: 858 459 79 566 2028 184 2007
Fragments arranged by size:
2028 2007 858 566 459 184 79
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2813 10
Size: 54 1425 343 981 3378
Fragments arranged by size:
3378 1425 981 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2997 4139 4498 4657 518
Size: 648 1831 1142 359 159 2042
Fragments arranged by size:
2042 1831 1142 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2724 2749 2997 3142 3597 4274 5422 5667
Size: 1118 25 248 145 455 677 1148 245
Cuts at: 5667 1606
Size: 2120
Fragments arranged by size:
2120 1148 1118 677 455 248 245 145
25
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4690
Fragments arranged by size:
4690 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6181
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6181
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 3539 5084 1132
Size: 97 2310 1545 2229
Fragments arranged by size:
2310 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2019 2656 3261 1348
Size: 671 637 605 4268
Fragments arranged by size:
4268 671 637 605
PciI A'CATG_T
Cuts at: 5080 5080
Size: 6181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2539 3042 3201 1108
Size: 1431 503 159 4088
Fragments arranged by size:
4088 1431 503 159
PfoI T'CCnGG_A
Cuts at: 2097 2610 2097
Size: 513 5668
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4250 4282 5763 5795 4250
Size: 32 1481 32 4636
Fragments arranged by size:
4636 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2764 2849 2764
Size: 85 6096
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PspOMI G'GGCC_C
Cuts at: 2022 2070 2079 2210 2454 3464 2022
Size: 48 9 131 244 1010 4739
Fragments arranged by size:
4739 1010 244 131 48 9
PstI C_TGCA'G
Cuts at: 830 3533 830
Size: 2703 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
PvuII CAG'CTG
Cuts at: 2997 2997
Size: 6181
RsrII CG'GwC_CG
Cuts at: 2719 2719
Size: 6181
SacI G_AGCT'C
Cuts at: 3135 3135
Size: 6181
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SanDI GG'GwC_CC
Cuts at: 2849 2849
Size: 6181
SapI GCTCTTCn'nnn_
Cuts at: 2783 2783
Size: 6181
SbfI CC_TGCA'GG
Cuts at: 3533 3533
Size: 6181
ScaI AGT'ACT
Cuts at: 4429 6141 4429
Size: 1712 4469
SexAI A'CCwGG_T
Cuts at: 2535 2535
Size: 6181
SfcI C'TryA_G
Cuts at: 826 1045 2699 3529 4664 5345 5536 826
Size: 219 1654 830 1135 681 191 1471
Fragments arranged by size:
1654 1471 1135 830 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2078 2078
Size: 6181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 3508 1872
Size: 1636 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4285 5186 5448 820
Size: 197 926 2342 901 262 1553
Fragments arranged by size:
2342 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3972 493
Size: 3479 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 3539 1132
Size: 2407 3774
SspI AAT'ATT
Cuts at: 5 52 4105 5
Size: 47 4053 2081
Fragments arranged by size:
4053 2081 47
StuI AGG'CCT
Cuts at: 1517 2586 3056 1517
Size: 1069 470 4642
Fragments arranged by size:
4642 1069 470
StyI C'CwwG_G
Cuts at: 513 1065 2004 3620 513
Size: 552 939 1616 3074
Fragments arranged by size:
3074 1616 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2669 3113 3372 4208 4225 4378 4563
Size: 1255 1089 444 259 836 17 153 185
Cuts at: 4563 5008 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1089 836 445 444 259 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4427 6139 96
Size: 274 80 33 51 1443 2450 1712 138
Fragments arranged by size:
2450 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2491 2523 3277 3309 6141 6173 2491
Size: 32 754 32 2832 32 2499
Fragments arranged by size:
2832 2499 754 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2717 1316
Size: 1401 4780
XbaI T'CTAG_A
Cuts at: 3517 3517
Size: 6181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2842 3250 2842
Size: 408 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6181
XmaI C'CCGG_G
Cuts at: 1870 3506 1870
Size: 1636 4545
XmnI GAAnn'nnTTC
Cuts at: 2798 4310 2798
Size: 1512 4669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2617 3989 276
Size: 53 83 186 936 1083 1372 2468
Fragments arranged by size:
2468 1372 1083 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI SacII SfoI SrfI SwaI