Restriction map for
pFN21AE5609
(Circular) (Six-base) MAPSORT of: pFN21AE5609.seq Check: 6612 from: 1 to: 6304
pFN21AE5609 6304 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2119 2119
Size: 6304
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4114 278
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2793 3647 1937
Size: 856 854 4594
Fragments arranged by size:
4594 856 854
Acc65I G'GTAC_C
Cuts at: 3625 3625
Size: 6304
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4367 5751 6019 1632
Size: 2735 1384 268 1917
Fragments arranged by size:
2735 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 5203 6180 1203
Size: 4000 977 1327
Fragments arranged by size:
4000 1327 977
AgeI A'CCGG_T
Cuts at: 5135 5135
Size: 6304
AhdI GACnn_n'nnGTC
Cuts at: 5033 5033
Size: 6304
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6304
AlwNI CAG_nnn'CTG
Cuts at: 4047 5619 4047
Size: 1572 4732
ApaLI G'TGCA_C
Cuts at: 4361 5517 4361
Size: 1156 5148
ApoI r'AATT_y
Cuts at: 1493 2084 2562 3092 3525 3613 3839 3991 6100
Size: 591 478 530 433 88 226 152 2109
Cuts at: 6100 1493
Size: 1697
Fragments arranged by size:
2109 1697 591 530 478 433 226 152
88
AseI AT'TA_AT
Cuts at: 160 1032 3338 4858 160
Size: 872 2306 1520 1606
Fragments arranged by size:
2306 1606 1520 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 3629 1870
Size: 73 1686 4545
Fragments arranged by size:
4545 1686 73
BamHI G'GATC_C
Cuts at: 3596 3634 4001 3596
Size: 38 367 5899
Fragments arranged by size:
5899 367 38
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2107 3625 5080 618
Size: 325 217 627 320 1518 1455 1842
Fragments arranged by size:
1842 1518 1455 627 325 320 217
BanII G_rGCy'C
Cuts at: 1391 2055 3623 1391
Size: 664 1568 4072
Fragments arranged by size:
4072 1568 664
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5333
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4495 4529 1903
Size: 34 2558 34 3678
Fragments arranged by size:
3678 2558 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3180 4196 5406 3180
Size: 1016 1210 4078
Fragments arranged by size:
4078 1210 1016
BclI T'GATC_A
Cuts at: 1548 2866 1548
Size: 1318 4986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4915 136
Size: 107 122 71 631 605 3243 1525
Fragments arranged by size:
3243 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2935 6299 2935
Size: 3364 2940
BlpI GC'TnA_GC
Cuts at: 3721 3721
Size: 6304
Bme1580I G_kGCm'C
Cuts at: 1790 4365 5521 1790
Size: 2575 1156 2573
Fragments arranged by size:
2575 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4035 4993 442
Size: 416 53 83 399 24 2618 958 1753
Fragments arranged by size:
2618 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4964 1239
Size: 3725 2579
Bpu10I CC'TnA_GC
Cuts at: 2501 2826 2501
Size: 325 5979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2916 4429 5294 5592 2916
Size: 1513 865 298 3628
Fragments arranged by size:
3628 1513 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4967 882
Size: 617 3468 2219
Fragments arranged by size:
3468 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3236 4095 493
Size: 711 2032 859 2702
Fragments arranged by size:
2702 2032 859 711
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4111 4493 5128 275
Size: 53 83 186 936 2578 382 635 1451
Fragments arranged by size:
2578 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4737 5135 5409 5556 5967 4737
Size: 398 274 147 411 5074
Fragments arranged by size:
5074 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6079
Fragments arranged by size:
6079 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 5373
BseYI C'CCAG_C
Cuts at: 5507 6003 5507
Size: 496 5808
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3404 1163
Size: 573 1668 4063
Fragments arranged by size:
4063 1668 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3754 4515 5543 1540
Size: 275 186 1753 761 1028 2301
Fragments arranged by size:
2301 1753 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2055 4365 4450 5521 2055
Size: 2310 85 1071 2838
Fragments arranged by size:
2838 2310 1071 85
BsmI GAATG_Cn'
Cuts at: 2206 3815 3908 2206
Size: 1609 93 4602
Fragments arranged by size:
4602 1609 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 3623 4365 4450 5521 1391
Size: 399 265 1568 742 85 1071 2174
Fragments arranged by size:
2174 1568 1071 742 399 265 85
BspEI T'CCGG_A
Cuts at: 5967 5967
Size: 6304
BspHI T'CATG_A
Cuts at: 2856 4193 5923 2856
Size: 1337 1730 3237
Fragments arranged by size:
3237 1730 1337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 3659 844
Size: 1275 1540 3489
Fragments arranged by size:
3489 1540 1275
BsrBI CCG'CTC
Cuts at: 1394 1823 4191 1394
Size: 429 2368 3507
Fragments arranged by size:
3507 2368 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4799 4973 65
Size: 1183 3551 174 1396
Fragments arranged by size:
3551 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4948 5135 1700
Size: 110 3138 187 2869
Fragments arranged by size:
3138 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 4364 5376 4364
Size: 1012 5292
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3140 794
Size: 2346 3958
BstBI TT'CG_AA
Cuts at: 3398 5108 3398
Size: 1710 4594
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6304
BstYI r'GATC_y
Cuts at: 1972 2935 3596 3634 4001 4386 4403 5855 5941
Size: 963 661 38 367 385 17 1452 86
Cuts at: 5941 5953 6299 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 963 661 385 367 346 86
38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3546 513
Size: 552 2481 3271
Fragments arranged by size:
3271 2481 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5565
Fragments arranged by size:
5565 683 56
BtsI GCAGTG_nn'
Cuts at: 2812 3820 4614 4634 2812
Size: 1008 794 20 4482
Fragments arranged by size:
4482 1008 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 2089 3608 2089
Size: 1519 4785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5311 809
Size: 507 3995 1802
Fragments arranged by size:
3995 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3751 4640 6269
Size: 54 1425 50 275 18 1921 889 1629
Cuts at: 6269 8
Size: 43
Fragments arranged by size:
1921 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3751 1537
Size: 275 1939 4090
Fragments arranged by size:
4090 1939 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3116 4234 1353
Size: 1763 1118 3423
Fragments arranged by size:
3423 1763 1118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4891 5265 5411 387
Size: 1064 138 3302 374 146 1280
Fragments arranged by size:
3302 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2053 2053
Size: 6304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4367 4964 5751 6019 1239
Size: 393 2735 597 787 268 1524
Fragments arranged by size:
2735 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3613 3991 3613
Size: 378 5926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2739 3292 2739
Size: 553 5751
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 5451 1678
Size: 3773 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2851 2883 3174 3206 3410
Size: 32 135 32 1279 32 291 32 204
Cuts at: 3410 3442 3514 3546 4945 4977 5019 5051 1373
Size: 32 72 32 1399 32 42 32 2626
Fragments arranged by size:
2626 1399 1279 291 204 135 72 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3648 3894 669
Size: 988 281 1710 246 3079
Fragments arranged by size:
3079 1710 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6304
HpaI GTT'AAC
Cuts at: 3894 3894
Size: 6304
KpnI G_GTAC'C
Cuts at: 3629 3629
Size: 6304
MfeI C'AATT_G
Cuts at: 2211 3421 3903 2211
Size: 1210 482 4612
Fragments arranged by size:
4612 1210 482
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2048 5418 5602 1305
Size: 743 3370 184 2007
Fragments arranged by size:
3370 2007 743 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4482
Fragments arranged by size:
4482 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2447 4262 4621 4780 518
Size: 648 1281 1815 359 159 2042
Fragments arranged by size:
2042 1815 1281 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3720 4397 5545 5790 1606
Size: 2114 677 1148 245 2120
Fragments arranged by size:
2120 2114 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5752
NdeI CA'TA_TG
Cuts at: 387 2179 387
Size: 1792 4512
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6304
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 3662 5207 1132
Size: 97 2433 1545 2229
Fragments arranged by size:
2433 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6304
PciI A'CATG_T
Cuts at: 5203 5203
Size: 6304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2711 1108
Size: 1603 4701
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4373 4405 5886 5918 4373
Size: 32 1481 32 4759
Fragments arranged by size:
4759 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3197 3874 3197
Size: 677 5627
PstI C_TGCA'G
Cuts at: 830 3656 830
Size: 2826 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
SacI G_AGCT'C
Cuts at: 2055 2055
Size: 6304
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 4552 6264 4552
Size: 1712 4592
SfcI C'TryA_G
Cuts at: 826 1045 2728 3652 4787 5468 5659 826
Size: 219 1683 924 1135 681 191 1471
Fragments arranged by size:
1683 1471 1135 924 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 3631 1872
Size: 1759 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2931 4408 5309 5571 820
Size: 197 926 988 1477 901 262 1553
Fragments arranged by size:
1553 1477 988 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4095 493
Size: 3602 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 3662 1132
Size: 2530 3774
SspI AAT'ATT
Cuts at: 5 52 2509 3336 4228 5
Size: 47 2457 827 892 2081
Fragments arranged by size:
2457 2081 892 827 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6304
StyI C'CwwG_G
Cuts at: 513 1065 2907 3743 513
Size: 552 1842 836 3074
Fragments arranged by size:
3074 1842 836 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4331 4348 4501 4686 5131 325
Size: 1255 2751 17 153 185 445 1498
Fragments arranged by size:
2751 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4550 6262 96
Size: 274 80 33 51 1443 2573 1712 138
Fragments arranged by size:
2573 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6264 6296 6264
Size: 32 6272
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6304
XbaI T'CTAG_A
Cuts at: 2761 3640 2761
Size: 879 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6304
XmaI C'CCGG_G
Cuts at: 1870 3629 1870
Size: 1759 4545
XmnI GAAnn'nnTTC
Cuts at: 3093 3301 4433 3093
Size: 208 1132 4964
Fragments arranged by size:
4964 1132 208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4112 276
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI