Restriction map for
pFN21AE5608
(Circular) (Six-base) MAPSORT of: pFN21AE5608.seq Check: 1104 from: 1 to: 6130
pFN21AE5608 6130 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3473 1937
Size: 1536 4594
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2360 4193 5577 5845 1632
Size: 728 1833 1384 268 1917
Fragments arranged by size:
1917 1833 1384 728 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 2453 2926 5029 6006 1203
Size: 1250 473 2103 977 1327
Fragments arranged by size:
2103 1327 1250 977 473
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6130
AlwNI CAG_nnn'CTG
Cuts at: 2021 3198 3422 3873 5445 2021
Size: 1177 224 451 1572 2706
Fragments arranged by size:
2706 1572 1177 451 224
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 3373 3439 3665 3817 5926 1493
Size: 1880 66 226 152 2109 1697
Fragments arranged by size:
2109 1880 1697 226 152 66
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2559 2942 3455 1870
Size: 73 616 383 513 4545
Fragments arranged by size:
4545 616 513 383 73
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2334 2436 2573 2759 3451
Size: 325 217 627 547 102 137 186 692
Cuts at: 3451 4906 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 692 627 547 325 217 186
137 102
BanII G_rGCy'C
Cuts at: 1391 2981 3449 1391
Size: 1590 468 4072
Fragments arranged by size:
4072 1590 468
BbeI G_GCGC'C
Cuts at: 2338 2338
Size: 6130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2953 928
Size: 971 1054 4105
Fragments arranged by size:
4105 1054 971
BbvCI CC'TCA_GC
Cuts at: 2298 2410 2298
Size: 112 6018
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2051 2976
Size: 107 122 71 631 605 337 42 925
Cuts at: 2976 4741 136
Size: 1765 1525
Fragments arranged by size:
1765 1525 925 631 605 337 122 107
71 42
BglII A'GATC_T
Cuts at: 2148 6125 2148
Size: 3977 2153
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 2439 4191 5347 1790
Size: 649 1752 1156 2573
Fragments arranged by size:
2573 1752 1156 649
BmgBI CAC'GTC
Cuts at: 1318 2608 1318
Size: 1290 4840
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3861 4819 442
Size: 416 53 83 399 24 2444 958 1753
Fragments arranged by size:
2444 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2167 2196 2167
Size: 29 6101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2137 2237 3143 3277 4790 1239
Size: 898 100 906 134 1513 2579
Fragments arranged by size:
2579 1513 906 898 134 100
Bpu10I CC'TnA_GC
Cuts at: 2298 2410 2298
Size: 112 6018
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2375 2899 3269 4255 5120 5418 2375
Size: 524 370 986 865 298 3087
Fragments arranged by size:
3087 986 865 524 370 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3062 4793 882
Size: 617 1563 1731 2219
Fragments arranged by size:
2219 1731 1563 617
BsaAI yAC'GTr
Cuts at: 493 1204 2830 2927 3921 493
Size: 711 1626 97 994 2702
Fragments arranged by size:
2702 1626 994 711 97
BsaBI GATnn'nnATC
Cuts at: 6124 6124
Size: 6130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2335 3937 4319 4954
Size: 53 83 186 936 802 1602 382 635
Cuts at: 4954 275
Size: 1451
Fragments arranged by size:
1602 1451 936 802 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4563 4961 5235 5382 5793 4563
Size: 398 274 147 411 4900
Fragments arranged by size:
4900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2777 2807 2963 2993 1178
Size: 30 165 30 1374 30 156 30 4315
Fragments arranged by size:
4315 1374 165 156 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2210 2213 2216 2219 2222 2225 2300
Size: 973 51 3 3 3 3 3 75
Cuts at: 2300 2303 2330 2617 2960 3023 3149 1186
Size: 3 27 287 343 63 126 4167
Fragments arranged by size:
4167 973 343 287 126 75 63 51
27 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2404 2903 2952 3317 3415 5333 5829 2404
Size: 499 49 365 98 1918 496 2705
Fragments arranged by size:
2705 1918 499 496 365 98 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2211 2850 1163
Size: 573 475 639 4443
Fragments arranged by size:
4443 639 573 475
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2791 3580 4341 5369 1540
Size: 275 186 790 789 761 1028 2301
Fragments arranged by size:
2301 1028 790 789 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3015 3336 4191 4276 5347 3015
Size: 321 855 85 1071 3798
Fragments arranged by size:
3798 1071 855 321 85
BsmI GAATG_Cn'
Cuts at: 2747 3641 3734 2747
Size: 894 93 5143
Fragments arranged by size:
5143 894 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2828 2828
Size: 6130
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2439 2981 3015 3336 3449 4191 4276
Size: 399 649 542 34 321 113 742 85
Cuts at: 4276 5347 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 649 542 399 321 113
85 34
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 2072 4774 4961 1700
Size: 110 231 31 2702 187 2869
Fragments arranged by size:
2869 2702 231 187 110 31
BsrGI T'GTAC_A
Cuts at: 96 2706 96
Size: 2610 3520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2873 794
Size: 2079 4051
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 2654 1184
Size: 1470 4660
BstYI r'GATC_y
Cuts at: 1972 2148 2394 2937 3460 3827 4212 4229 5681
Size: 176 246 543 523 367 385 17 1452
Cuts at: 5681 5767 5779 6125 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 543 523 385 367 346 246
176 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2193 2193
Size: 6130
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2739 3221 3646 4440 4460 2739
Size: 482 425 794 20 4409
Fragments arranged by size:
4409 794 482 425 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DraIII CAC_nnn'GTG
Cuts at: 3019 3019
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2593 2619
Size: 54 1425 50 275 18 420 343 26
Cuts at: 2619 2968 3177 3343 3577 4466 6095 8
Size: 349 209 166 234 889 1629 43
Fragments arranged by size:
1629 1425 889 420 349 343 275 234
209 166 54 50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2846 4060 1353
Size: 1493 1214 3423
Fragments arranged by size:
3423 1493 1214
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2137 2237 2360 3143 3277 4193 4790
Size: 393 505 100 123 783 134 916 597
Cuts at: 4790 5577 5845 1239
Size: 787 268 1524
Fragments arranged by size:
1524 916 787 783 597 505 393 268
134 123 100
EcoNI CCTnn'n_nnAGG
Cuts at: 2342 2342
Size: 6130
EcoO109I rG'GnC_Cy
Cuts at: 2401 3403 2401
Size: 1002 5128
EcoRI G'AATT_C
Cuts at: 3373 3439 3817 3373
Size: 66 378 5686
Fragments arranged by size:
5686 378 66
EcoRV GAT'ATC
Cuts at: 3141 3141
Size: 6130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 6130
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 2338 5277 1678
Size: 660 2939 2531
Fragments arranged by size:
2939 2531 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2720 2752 3023 3055 4771
Size: 32 135 32 1148 32 271 32 1716
Cuts at: 4771 4803 4845 4877 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1716 1148 271 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3474 3720 669
Size: 988 281 1536 246 3079
Fragments arranged by size:
3079 1536 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6130
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KasI G'GCGC_C
Cuts at: 2334 2334
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 5244 5428 1305
Size: 1350 2589 184 2007
Fragments arranged by size:
2589 2007 1350 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2252 2595 2621 2970 3179
Size: 54 1425 343 420 343 26 349 209
Cuts at: 3179 10
Size: 2961
Fragments arranged by size:
2961 1425 420 349 343 343 209 54
26
MslI CAynn'nnrTG
Cuts at: 518 1166 2727 4088 4447 4606 518
Size: 648 1561 1361 359 159 2042
Fragments arranged by size:
2042 1561 1361 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2414 3419 3546 4223 5371 5616 1606
Size: 412 396 1005 127 677 1148 245 2120
Fragments arranged by size:
2120 1148 1005 677 412 396 245 127
NaeI GCC'GGC
Cuts at: 2043 2074 2043
Size: 31 6099
NarI GG'CG_CC
Cuts at: 2335 2335
Size: 6130
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5578
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NgoMIV G'CCGG_C
Cuts at: 2041 2072 2041
Size: 31 6099
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2457 2552 3488 5033 1132
Size: 97 1228 95 936 1545 2229
Fragments arranged by size:
2229 1545 1228 936 97 95
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 2453 5029 2453
Size: 2576 3554
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3422 1108
Size: 2314 3816
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3403 3403
Size: 6130
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PspXI vC'TCGA_Gb
Cuts at: 2559 2559
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2376 2510 3087 3482 830
Size: 1546 134 577 395 3478
Fragments arranged by size:
3478 1546 577 395 134
PvuI CG_AT'CG
Cuts at: 2001 2791 2001
Size: 790 5340
PvuII CAG'CTG
Cuts at: 2414 3419 2414
Size: 1005 5125
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SanDI GG'GwC_CC
Cuts at: 3403 3403
Size: 6130
SapI GCTCTTCn'nnn_
Cuts at: 2846 2846
Size: 6130
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SexAI A'CCwGG_T
Cuts at: 2322 2322
Size: 6130
SfcI C'TryA_G
Cuts at: 826 1045 2372 2506 2773 3083 3478 4613 5294
Size: 219 1327 134 267 310 395 1135 681
Cuts at: 5294 5485 826
Size: 191 1471
Fragments arranged by size:
1471 1327 1135 681 395 310 267 219
191 134
SfiI GGCCn_nnn'nGGCC
Cuts at: 2051 2051
Size: 6130
SfoI GGC'GCC
Cuts at: 2336 2336
Size: 6130
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2354 2559 2878 3284 4234 5135
Size: 197 926 411 205 319 406 950 901
Cuts at: 5135 5397 820
Size: 262 1553
Fragments arranged by size:
1553 950 926 901 411 406 319 262
205 197
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 3120 1517
Size: 1603 4527
StyI C'CwwG_G
Cuts at: 513 1065 2121 2183 3569 513
Size: 552 1056 62 1386 3074
Fragments arranged by size:
3074 1386 1056 552 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2777 4157 4174 4327 4512 4957 325
Size: 1255 1197 1380 17 153 185 445 1498
Fragments arranged by size:
1498 1380 1255 1197 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 4376 6088
Size: 274 80 33 51 1443 729 1670 1712
Cuts at: 6088 96
Size: 138
Fragments arranged by size:
1712 1670 1443 729 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2777 2809 2911 2943 2963 2995 3005 3037 6090
Size: 32 102 32 20 32 10 32 3053
Cuts at: 6090 6122 2777
Size: 32 2785
Fragments arranged by size:
3053 2785 102 32 32 32 32 32
20 10
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 2559 1943
Size: 616 5514
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstXI BstZ17I EcoICRI FseI FspAI NruI NsiI
PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII
SrfI SwaI XcmI