Restriction map for
pFN21AE5607
(Circular) (Six-base) MAPSORT of: pFN21AE5607.seq Check: 7756 from: 1 to: 6463
pFN21AE5607 6463 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4273 278
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3806 1937
Size: 1869 4594
Acc65I G'GTAC_C
Cuts at: 3784 3784
Size: 6463
AclI AA'CG_TT
Cuts at: 1563 4590 4963 1563
Size: 3027 373 3063
Fragments arranged by size:
3063 3027 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2193 3042 3207 3711 4526 5910 6178 1632
Size: 561 849 165 504 815 1384 268 1917
Fragments arranged by size:
1917 1384 849 815 561 504 268 165
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6266
AflIII A'CryG_T
Cuts at: 1203 2464 5362 6339 1203
Size: 1261 2898 977 1327
Fragments arranged by size:
2898 1327 1261 977
AgeI A'CCGG_T
Cuts at: 5294 5294
Size: 6463
AhdI GACnn_n'nnGTC
Cuts at: 5192 5192
Size: 6463
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6463
AlwNI CAG_nnn'CTG
Cuts at: 4206 5778 4206
Size: 1572 4891
ApaLI G'TGCA_C
Cuts at: 4520 5676 4520
Size: 1156 5307
ApoI r'AATT_y
Cuts at: 1493 2329 2432 2680 2761 2833 2845 3772 3998
Size: 836 103 248 81 72 12 927 226
Cuts at: 3998 4150 6259 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 927 836 248 226 152 103
81 72 12
AseI AT'TA_AT
Cuts at: 160 1032 5017 160
Size: 872 3985 1606
Fragments arranged by size:
3985 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
AvaI C'yCGr_G
Cuts at: 1870 1943 3678 3788 1870
Size: 73 1735 110 4545
Fragments arranged by size:
4545 1735 110 73
AvrII C'CTAG_G
Cuts at: 2414 2414
Size: 6463
BamHI G'GATC_C
Cuts at: 3793 4160 3793
Size: 367 6096
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3784 5239 618
Size: 325 217 627 1997 1455 1842
Fragments arranged by size:
1997 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3782 1391
Size: 2391 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3195 928
Size: 971 1296 4196
Fragments arranged by size:
4196 1296 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2028 2062 4654 4688 1903
Size: 34 91 34 2592 34 3678
Fragments arranged by size:
3678 2592 91 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3323 4355 5565 3323
Size: 1032 1210 4221
Fragments arranged by size:
4221 1210 1032
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6463
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5074 136
Size: 107 122 71 631 605 3402 1525
Fragments arranged by size:
3402 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6458 6458
Size: 6463
BlpI GC'TnA_GC
Cuts at: 3880 3880
Size: 6463
Bme1580I G_kGCm'C
Cuts at: 1790 4524 5680 1790
Size: 2734 1156 2573
Fragments arranged by size:
2734 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6463
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3438 4194 5152
Size: 416 53 83 399 24 2021 756 958
Cuts at: 5152 442
Size: 1753
Fragments arranged by size:
2021 1753 958 756 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2122 2319 2439 2955 3144 3393 5123 1239
Size: 883 197 120 516 189 249 1730 2579
Fragments arranged by size:
2579 1730 883 516 249 197 189 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3190 4588 5453 5751 3190
Size: 1398 865 298 3902
Fragments arranged by size:
3902 1398 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2254 2404 3620 5126 882
Size: 617 755 150 1216 1506 2219
Fragments arranged by size:
2219 1506 1216 755 617 150
BsaAI yAC'GTr
Cuts at: 493 1204 4254 493
Size: 711 3050 2702
Fragments arranged by size:
3050 2702 711
BsaBI GATnn'nnATC
Cuts at: 6457 6457
Size: 6463
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4270 4652 5287 275
Size: 53 83 186 936 2737 382 635 1451
Fragments arranged by size:
2737 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2790 4896 5294 5568 5715 6126 2790
Size: 2106 398 274 147 411 3127
Fragments arranged by size:
3127 2106 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6238
Fragments arranged by size:
6238 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3278 3419 3614 1186
Size: 2092 141 195 4035
Fragments arranged by size:
4035 2092 195 141
BseYI C'CCAG_C
Cuts at: 3380 5666 6162 3380
Size: 2286 496 3681
Fragments arranged by size:
3681 2286 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2022 1163
Size: 573 286 5604
Fragments arranged by size:
5604 573 286
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3913 4674 5702 1540
Size: 275 186 1912 761 1028 2301
Fragments arranged by size:
2301 1912 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4524 4609 5680 4524
Size: 85 1071 5307
Fragments arranged by size:
5307 1071 85
BsmI GAATG_Cn'
Cuts at: 3974 4067 3974
Size: 93 6370
BsmBI CGTCTCn'nnnn_
Cuts at: 2770 2770
Size: 6463
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3782 4524 4609 5680 1391
Size: 399 1992 742 85 1071 2174
Fragments arranged by size:
2174 1992 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2790 6126 2790
Size: 3336 3127
BspHI T'CATG_A
Cuts at: 4352 6082 4352
Size: 1730 4733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3818 844
Size: 2974 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3238 4350 1394
Size: 429 1415 1112 3507
Fragments arranged by size:
3507 1415 1112 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2149 2883 4958 5132 65
Size: 1183 901 734 2075 174 1396
Fragments arranged by size:
2075 1396 1183 901 734 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5107 5294 1700
Size: 110 3297 187 2869
Fragments arranged by size:
3297 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6463
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6463
BssSI C'ACGA_G
Cuts at: 4523 5535 4523
Size: 1012 5451
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2033 794
Size: 1239 5224
BstBI TT'CG_AA
Cuts at: 5267 5267
Size: 6463
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6463
BstXI CCAn_nnnn'nTGG
Cuts at: 2974 2974
Size: 6463
BstYI r'GATC_y
Cuts at: 1972 2839 3017 3040 3276 3793 4160 4545 4562
Size: 867 178 23 236 517 367 385 17
Cuts at: 4562 6014 6100 6112 6458 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 867 517 385 367 346 236
178 86 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 2620 2620
Size: 6463
BtgI C'CryG_G
Cuts at: 513 1065 2216 3741 513
Size: 552 1151 1525 3235
Fragments arranged by size:
3235 1525 1151 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5724
Fragments arranged by size:
5724 683 56
BtsI GCAGTG_nn'
Cuts at: 3979 4773 4793 3979
Size: 794 20 5649
Fragments arranged by size:
5649 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6428
DraI TTT'AAA
Cuts at: 2250 3767 2250
Size: 1517 4946
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5470 809
Size: 507 4154 1802
Fragments arranged by size:
4154 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3136 3910 4799
Size: 54 1425 50 275 18 1306 774 889
Cuts at: 4799 6428 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1306 889 774 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3910 1537
Size: 275 2098 4090
Fragments arranged by size:
4090 2098 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2495 2547 2664 3687 4393 1353
Size: 1142 52 117 1023 706 3423
Fragments arranged by size:
3423 1142 1023 706 117 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5050 5424 5570 387
Size: 1064 138 3461 374 146 1280
Fragments arranged by size:
3461 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2193 2319 2439 2955 3042 3144
Size: 393 490 71 126 120 516 87 102
Cuts at: 3144 3207 3393 3711 4526 5123 5910 6178 1239
Size: 63 186 318 815 597 787 268 1524
Fragments arranged by size:
1524 815 787 597 516 490 393 318
268 186 126 120 102 87 71 63
EcoNI CCTnn'n_nnAGG
Cuts at: 3758 3758
Size: 6463
EcoRI G'AATT_C
Cuts at: 2329 3772 4150 2329
Size: 1443 378 4642
Fragments arranged by size:
4642 1443 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2262 2262
Size: 6463
FspI TGC'GCA
Cuts at: 4969 4969
Size: 6463
HaeII r_GCGC'y
Cuts at: 1678 5610 1678
Size: 3932 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2872 2904 3002 3034 3268
Size: 32 135 32 1300 32 98 32 234
Cuts at: 3268 3300 5104 5136 5178 5210 1373
Size: 32 1804 32 42 32 2626
Fragments arranged by size:
2626 1804 1300 234 135 98 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3397 3807 4053 669
Size: 988 281 1459 410 246 3079
Fragments arranged by size:
3079 1459 988 410 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6463
HpaI GTT'AAC
Cuts at: 3397 4053 3397
Size: 656 5807
KpnI G_GTAC'C
Cuts at: 3788 3788
Size: 6463
MfeI C'AATT_G
Cuts at: 4062 4062
Size: 6463
MluI A'CGCG_T
Cuts at: 6339 6339
Size: 6463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2404 5577 5761 1305
Size: 1099 3173 184 2007
Fragments arranged by size:
3173 2007 1099 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3138 10
Size: 54 1425 343 1306 3335
Fragments arranged by size:
3335 1425 1306 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2706 2972 3150 4421 4780 4939 518
Size: 648 1540 266 178 1271 359 159 2042
Fragments arranged by size:
2042 1540 1271 648 359 266 178 159
MspA1I CmG'CkG
Cuts at: 1606 3164 3879 4556 5704 5949 1606
Size: 1558 715 677 1148 245 2120
Fragments arranged by size:
2120 1558 1148 715 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2216 3741 513
Size: 552 1151 1525 3235
Fragments arranged by size:
3235 1525 1151 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6463
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6463
NotI GC'GGCC_GC
Cuts at: 3910 3910
Size: 6463
NspI r_CATG'y
Cuts at: 1132 1229 2364 2468 2752 3670 3821 5366 1132
Size: 97 1135 104 284 918 151 1545 2229
Fragments arranged by size:
2229 1545 1135 918 284 151 104 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6463
PciI A'CATG_T
Cuts at: 2464 5362 2464
Size: 2898 3565
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6463
PfoI T'CCnGG_A
Cuts at: 3120 3120
Size: 6463
PmeI GTTT'AAAC
Cuts at: 3767 3767
Size: 6463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4532 4564 6045 6077 4532
Size: 32 1481 32 4918
Fragments arranged by size:
4918 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6463
PsiI TTA'TAA
Cuts at: 3416 4033 3416
Size: 617 5846
PstI C_TGCA'G
Cuts at: 830 2155 3815 830
Size: 1325 1660 3478
Fragments arranged by size:
3478 1660 1325
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6463
PvuII CAG'CTG
Cuts at: 3164 3164
Size: 6463
SalI G'TCGA_C
Cuts at: 1936 3805 1936
Size: 1869 4594
SbfI CC_TGCA'GG
Cuts at: 3815 3815
Size: 6463
ScaI AGT'ACT
Cuts at: 3203 4711 6423 3203
Size: 1508 1712 3243
Fragments arranged by size:
3243 1712 1508
SfcI C'TryA_G
Cuts at: 826 1045 2151 3032 3811 4946 5627 5818 826
Size: 219 1106 881 779 1135 681 191 1471
Fragments arranged by size:
1471 1135 1106 881 779 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6463
SmaI CCC'GGG
Cuts at: 1872 3790 1872
Size: 1918 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3205 3678 4567 5468 5730 820
Size: 197 926 1262 473 889 901 262 1553
Fragments arranged by size:
1553 1262 926 901 889 473 262 197
SnaBI TAC'GTA
Cuts at: 493 4254 493
Size: 3761 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6463
SphI G_CATG'C
Cuts at: 1132 3821 1132
Size: 2689 3774
SspI AAT'ATT
Cuts at: 5 52 2825 4387 5
Size: 47 2773 1562 2081
Fragments arranged by size:
2773 2081 1562 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6463
StyI C'CwwG_G
Cuts at: 513 1065 2216 2414 3741 3754 3902 513
Size: 552 1151 198 1327 13 148 3074
Fragments arranged by size:
3074 1327 1151 552 198 148 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4490 4507 4660 4845 5290 325
Size: 1255 2910 17 153 185 445 1498
Fragments arranged by size:
2910 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3201 3368 4709
Size: 274 80 33 51 1443 1224 167 1341
Cuts at: 4709 6421 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1341 1224 274 167 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2616 2648 3009 3041 6423 6455 2616
Size: 32 361 32 3382 32 2624
Fragments arranged by size:
3382 2624 361 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6463
XbaI T'CTAG_A
Cuts at: 3799 3799
Size: 6463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2569 2658 2569
Size: 89 6374
XhoI C'TCGA_G
Cuts at: 1943 3678 1943
Size: 1735 4728
XmaI C'CCGG_G
Cuts at: 1870 3788 1870
Size: 1918 4545
XmnI GAAnn'nnTTC
Cuts at: 4592 4592
Size: 6463
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4271 276
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI