Restriction map for
pFN21AE5604
(Circular) (Six-base) MAPSORT of: pFN21AE5604.seq Check: 5053 from: 1 to: 6136
pFN21AE5604 6136 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 1937
Size: 1542 4594
Acc65I G'GTAC_C
Cuts at: 2051 2532 2952 3457 2051
Size: 481 420 505 4730
Fragments arranged by size:
4730 505 481 420
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2978 3413 4199 5583 5851 1632
Size: 1346 435 786 1384 268 1917
Fragments arranged by size:
1917 1384 1346 786 435 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5939
AflIII A'CryG_T
Cuts at: 1203 5035 6012 1203
Size: 3832 977 1327
Fragments arranged by size:
3832 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 4865 4865
Size: 6136
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6136
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2799 2831 2799
Size: 32 6104
AlwNI CAG_nnn'CTG
Cuts at: 2548 3879 5451 2548
Size: 1331 1572 3233
Fragments arranged by size:
3233 1572 1331
ApaLI G'TGCA_C
Cuts at: 4193 5349 4193
Size: 1156 4980
ApoI r'AATT_y
Cuts at: 1493 3203 3406 3445 3671 3823 5932 1493
Size: 1710 203 39 226 152 2109 1697
Fragments arranged by size:
2109 1710 1697 226 203 152 39
AscI GG'CGCG_CC
Cuts at: 2354 2354
Size: 6136
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 2101 2136 2368 2636 3273 3461 1870
Size: 73 158 35 232 268 637 188 4545
Fragments arranged by size:
4545 637 268 232 188 158 73 35
AvrII C'CTAG_G
Cuts at: 2440 2440
Size: 6136
BamHI G'GATC_C
Cuts at: 3466 3833 3466
Size: 367 5769
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2051 2345 2532 2952
Size: 325 217 627 224 40 294 187 420
Cuts at: 2952 3457 4912 618
Size: 505 1455 1842
Fragments arranged by size:
1842 1455 627 505 420 325 294 224
217 187 40
BanII G_rGCy'C
Cuts at: 1391 2187 2196 2434 2636 3455 1391
Size: 796 9 238 202 819 4072
Fragments arranged by size:
4072 819 796 238 202 9
BbeI G_GCGC'C
Cuts at: 2015 2349 2015
Size: 334 5802
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2117 2151 4327 4361 1903
Size: 34 180 34 2176 34 3678
Fragments arranged by size:
3678 2176 180 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 2740 2992 3160 1548
Size: 1192 252 168 4524
Fragments arranged by size:
4524 1192 252 168
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2083 2459
Size: 107 122 71 631 605 337 74 376
Cuts at: 2459 2640 3028 4747 136
Size: 181 388 1719 1525
Fragments arranged by size:
1719 1525 631 605 388 376 337 181
122 107 74 71
BglII A'GATC_T
Cuts at: 6131 6131
Size: 6136
BlpI GC'TnA_GC
Cuts at: 2600 3553 2600
Size: 953 5183
Bme1580I G_kGCm'C
Cuts at: 1790 4197 5353 1790
Size: 2407 1156 2573
Fragments arranged by size:
2573 2407 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6136
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3867 4825 442
Size: 416 53 83 399 24 2450 958 1753
Fragments arranged by size:
2450 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2660 2811 2859 3167 4796 1239
Size: 1181 240 151 48 308 1629 2579
Fragments arranged by size:
2579 1629 1181 308 240 151 48
Bpu10I CC'TnA_GC
Cuts at: 2109 2109
Size: 6136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2612 3353 4261 5126 5424 2468
Size: 144 741 908 865 298 3180
Fragments arranged by size:
3180 908 865 741 298 144
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2358 4799 882
Size: 617 859 2441 2219
Fragments arranged by size:
2441 2219 859 617
BsaAI yAC'GTr
Cuts at: 493 1204 3927 493
Size: 711 2723 2702
Fragments arranged by size:
2723 2702 711
BsaBI GATnn'nnATC
Cuts at: 6130 6130
Size: 6136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2346 3943 4325
Size: 53 83 186 936 479 334 1597 382
Cuts at: 4325 4960 275
Size: 635 1451
Fragments arranged by size:
1597 1451 936 635 479 382 334 186
83 53
BsaWI w'CCGG_w
Cuts at: 2762 2932 4569 4967 5241 5388 5799 2762
Size: 170 1637 398 274 147 411 3099
Fragments arranged by size:
3099 1637 411 398 274 170 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2784 2814 3140 3170 1178
Size: 30 165 30 1381 30 326 30 4144
Fragments arranged by size:
4144 1381 326 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6136
BseYI C'CCAG_C
Cuts at: 2290 2728 2848 5339 5835 2290
Size: 438 120 2491 496 2591
Fragments arranged by size:
2591 2491 496 438 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2636 1163
Size: 573 900 4663
Fragments arranged by size:
4663 900 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2203 2377 3586 4347 5375 1540
Size: 275 186 202 174 1209 761 1028 2301
Fragments arranged by size:
2301 1209 1028 761 275 202 186 174
BsiHKAI G_wGCw'C
Cuts at: 2187 2196 2434 2676 4197 4282 5353 2187
Size: 9 238 242 1521 85 1071 2970
Fragments arranged by size:
2970 1521 1071 242 238 85 9
BsmI GAATG_Cn'
Cuts at: 3647 3740 3647
Size: 93 6043
BsmBI CGTCTCn'nnnn_
Cuts at: 2159 2159
Size: 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2196 2434 2636 2676 3455 4197
Size: 399 397 9 238 202 40 779 742
Cuts at: 4197 4282 5353 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 779 742 399 397 238 202
85 40 9
BspEI T'CCGG_A
Cuts at: 2762 5799 2762
Size: 3037 3099
BspHI T'CATG_A
Cuts at: 4025 5755 4025
Size: 1730 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3491 844
Size: 2647 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2315 2373 4023 1394
Size: 429 492 58 1650 3507
Fragments arranged by size:
3507 1650 492 429 58
BsrDI GCAATG_nn'
Cuts at: 65 1248 4631 4805 65
Size: 1183 3383 174 1396
Fragments arranged by size:
3383 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 2604 4780 4967 1700
Size: 110 230 564 2176 187 2869
Fragments arranged by size:
2869 2176 564 230 187 110
BsrGI T'GTAC_A
Cuts at: 96 3110 96
Size: 3014 3122
BssHII G'CGCG_C
Cuts at: 1925 2354 1925
Size: 429 5707
BssSI C'ACGA_G
Cuts at: 4196 5208 4196
Size: 1012 5124
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2614 794
Size: 1820 4316
BstBI TT'CG_AA
Cuts at: 4940 4940
Size: 6136
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6136
BstXI CCAn_nnnn'nTGG
Cuts at: 2711 2711
Size: 6136
BstYI r'GATC_y
Cuts at: 1972 2735 3466 3833 4218 4235 5687 5773 5785
Size: 763 731 367 385 17 1452 86 12
Cuts at: 5785 6131 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 763 731 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2203 2250 2349 513
Size: 552 939 199 47 99 4300
Fragments arranged by size:
4300 939 552 199 99 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5397
Fragments arranged by size:
5397 683 56
BtsI GCAGTG_nn'
Cuts at: 2574 3652 4446 4466 2574
Size: 1078 794 20 4244
Fragments arranged by size:
4244 1078 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3184 3440 3184
Size: 256 5880
DraIII CAC_nnn'GTG
Cuts at: 2326 2551 2326
Size: 225 5911
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5143 809
Size: 507 3827 1802
Fragments arranged by size:
3827 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2374 2652 3583
Size: 54 1425 50 275 18 544 278 931
Cuts at: 3583 4472 6101 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 931 889 544 278 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2374 3583 1537
Size: 275 562 1209 4090
Fragments arranged by size:
4090 1209 562 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2705 4066 1353
Size: 848 504 1361 3423
Fragments arranged by size:
3423 1361 848 504
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2748 3315 4723 5097 5243 387
Size: 1064 138 1159 567 1408 374 146 1280
Fragments arranged by size:
1408 1280 1159 1064 567 374 146 138
EcoICRI GAG'CTC
Cuts at: 2185 2194 2432 2185
Size: 9 238 5889
Fragments arranged by size:
5889 238 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2660 2811 2859 2978 3167 3413
Size: 393 788 240 151 48 119 189 246
Cuts at: 3413 4199 4796 5583 5851 1239
Size: 786 597 787 268 1524
Fragments arranged by size:
1524 788 787 786 597 393 268 246
240 189 151 119 48
EcoO109I rG'GnC_Cy
Cuts at: 2452 3035 2452
Size: 583 5553
EcoRI G'AATT_C
Cuts at: 3445 3823 3445
Size: 378 5758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 6136
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 2015 2109 2349 2399 2494 5283 1678
Size: 337 94 240 50 95 2789 2531
Fragments arranged by size:
2789 2531 337 240 95 94 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 2706 2738 2879
Size: 32 135 32 905 32 197 32 141
Cuts at: 2879 2911 3140 3172 4777 4809 4851 4883 1373
Size: 32 229 32 1605 32 42 32 2626
Fragments arranged by size:
2626 1605 905 229 197 141 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2787 3480 3726 669
Size: 988 281 849 693 246 3079
Fragments arranged by size:
3079 988 849 693 281 246
HindIII A'AGCT_T
Cuts at: 748 3068 748
Size: 2320 3816
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KasI G'GCGC_C
Cuts at: 2011 2345 2011
Size: 334 5802
KpnI G_GTAC'C
Cuts at: 2055 2536 2956 3461 2055
Size: 481 420 505 4730
Fragments arranged by size:
4730 505 481 420
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3334 5250 5434 1305
Size: 2029 1916 184 2007
Fragments arranged by size:
2029 2007 1916 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4314
Fragments arranged by size:
4314 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4094 4453 4612 518
Size: 648 2928 359 159 2042
Fragments arranged by size:
2928 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2620 2728 3007 3552 4229 5377 5622 1606
Size: 1014 108 279 545 677 1148 245 2120
Fragments arranged by size:
2120 1148 1014 677 545 279 245 108
NaeI GCC'GGC
Cuts at: 2606 2606
Size: 6136
NarI GG'CG_CC
Cuts at: 2012 2346 2012
Size: 334 5802
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4645
Fragments arranged by size:
4645 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NgoMIV G'CCGG_C
Cuts at: 2604 2604
Size: 6136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 3014 3494 5039 1132
Size: 97 1785 480 1545 2229
Fragments arranged by size:
2229 1785 1545 480 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6136
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6136
PfoI T'CCnGG_A
Cuts at: 2160 3217 2160
Size: 1057 5079
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3035 3035
Size: 6136
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6136
PsiI TTA'TAA
Cuts at: 3419 3706 3419
Size: 287 5849
PspXI vC'TCGA_Gb
Cuts at: 2136 2136
Size: 6136
PstI C_TGCA'G
Cuts at: 830 3488 830
Size: 2658 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
PvuII CAG'CTG
Cuts at: 2620 2728 3007 2620
Size: 108 279 5749
Fragments arranged by size:
5749 279 108
SacI G_AGCT'C
Cuts at: 2187 2196 2434 2187
Size: 9 238 5889
Fragments arranged by size:
5889 238 9
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SapI GCTCTTCn'nnn_
Cuts at: 2201 2201
Size: 6136
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 4384 6096 4384
Size: 1712 4424
SexAI A'CCwGG_T
Cuts at: 2547 2667 2547
Size: 120 6016
SfcI C'TryA_G
Cuts at: 826 1045 3484 4619 5300 5491 826
Size: 219 2439 1135 681 191 1471
Fragments arranged by size:
2439 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2013 2347 2013
Size: 334 5802
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 3463 1872
Size: 1591 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2136 2447 2627 3368 4240 5141
Size: 197 926 193 311 180 741 872 901
Cuts at: 5141 5403 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 872 741 311 262 197
193 180
SnaBI TAC'GTA
Cuts at: 493 3927 493
Size: 3434 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 2681 1517
Size: 1164 4972
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 2382 2440 3575 513
Size: 552 939 75 303 58 1135 3074
Fragments arranged by size:
3074 1135 939 552 303 75 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4163 4180 4333 4518 4963 325
Size: 1255 2583 17 153 185 445 1498
Fragments arranged by size:
2583 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2831 3110 4382
Size: 274 80 33 51 1443 854 279 1272
Cuts at: 4382 6094 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1272 854 279 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6096 6128 6096
Size: 32 6104
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6136
XbaI T'CTAG_A
Cuts at: 3472 3472
Size: 6136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2356
Size: 6136
XhoI C'TCGA_G
Cuts at: 1943 2136 1943
Size: 193 5943
XmaI C'CCGG_G
Cuts at: 1870 3461 1870
Size: 1591 4545
XmnI GAAnn'nnTTC
Cuts at: 2963 3207 4265 2963
Size: 244 1058 4834
Fragments arranged by size:
4834 1058 244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI BaeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI
PacI PmlI PspOMI PsrI RsrII SacII SanDI SfiI
SrfI SwaI