Restriction map for
pFN21AE5601
(Circular) (Six-base) MAPSORT of: pFN21AE5601.seq Check: 250 from: 1 to: 6157
pFN21AE5601 6157 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3967 278
Size: 53 83 186 936 2431 2468
Fragments arranged by size:
2468 2431 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3129 3500 1937
Size: 1192 371 4594
Fragments arranged by size:
4594 1192 371
Acc65I G'GTAC_C
Cuts at: 3478 3478
Size: 6157
AclI AA'CG_TT
Cuts at: 1563 4284 4657 1563
Size: 2721 373 3063
Fragments arranged by size:
3063 2721 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4220 5604 5872 1632
Size: 2588 1384 268 1917
Fragments arranged by size:
2588 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5960
AflIII A'CryG_T
Cuts at: 1203 2353 5056 6033 1203
Size: 1150 2703 977 1327
Fragments arranged by size:
2703 1327 1150 977
AgeI A'CCGG_T
Cuts at: 4988 4988
Size: 6157
AhdI GACnn_n'nnGTC
Cuts at: 4886 4886
Size: 6157
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6157
AlwNI CAG_nnn'CTG
Cuts at: 2026 2405 2674 3172 3900 5472 2026
Size: 379 269 498 728 1572 2711
Fragments arranged by size:
2711 1572 728 498 379 269
ApaLI G'TGCA_C
Cuts at: 4214 5370 4214
Size: 1156 5001
ApoI r'AATT_y
Cuts at: 1493 3043 3466 3692 3844 5953 1493
Size: 1550 423 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 423 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4711 160
Size: 872 3679 1606
Fragments arranged by size:
3679 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2076 3482 1870
Size: 73 104 29 1406 4545
Fragments arranged by size:
4545 1406 104 73 29
BamHI G'GATC_C
Cuts at: 2521 3487 3854 2521
Size: 966 367 4824
Fragments arranged by size:
4824 966 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2125 2622 3478 4933
Size: 325 217 627 221 117 497 856 1455
Cuts at: 4933 618
Size: 1842
Fragments arranged by size:
1842 1455 856 627 497 325 221 217
117
BanII G_rGCy'C
Cuts at: 1391 2346 2415 2438 2973 3476 1391
Size: 955 69 23 535 503 4072
Fragments arranged by size:
4072 955 535 503 69 23
BbeI G_GCGC'C
Cuts at: 2012 2129 2626 2012
Size: 117 497 5543
Fragments arranged by size:
5543 497 117
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2356 928
Size: 971 457 4729
Fragments arranged by size:
4729 971 457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4348 4382 1903
Size: 34 2411 34 3678
Fragments arranged by size:
3678 2411 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4049 5259 4049
Size: 1210 4947
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6157
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2220 3032 3185
Size: 107 122 71 631 605 548 812 153
Cuts at: 3185 3443 4768 136
Size: 258 1325 1525
Fragments arranged by size:
1525 1325 812 631 605 548 258 153
122 107 71
BglII A'GATC_T
Cuts at: 6152 6152
Size: 6157
BlpI GC'TnA_GC
Cuts at: 2437 3152 3574 2437
Size: 715 422 5020
Fragments arranged by size:
5020 715 422
Bme1580I G_kGCm'C
Cuts at: 1790 3368 4218 5374 1790
Size: 1578 850 1156 2573
Fragments arranged by size:
2573 1578 1156 850
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6157
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2375 3382 3888
Size: 416 53 83 399 24 958 1007 506
Cuts at: 3888 4846 442
Size: 958 1753
Fragments arranged by size:
1753 1007 958 958 506 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2264 2276 2854 3077 4817 1239
Size: 926 99 12 578 223 1740 2579
Fragments arranged by size:
2579 1740 926 578 223 99 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4282 5147 5445 4282
Size: 865 298 4994
Fragments arranged by size:
4994 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4820 882
Size: 617 3321 2219
Fragments arranged by size:
3321 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2356 3948 493
Size: 711 1152 1592 2702
Fragments arranged by size:
2702 1592 1152 711
BsaBI GATnn'nnATC
Cuts at: 6151 6151
Size: 6157
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2126 2623 3964
Size: 53 83 186 936 476 117 497 1341
Cuts at: 3964 4346 4981 275
Size: 382 635 1451
Fragments arranged by size:
1451 1341 936 635 497 476 382 186
117 83 53
BsaWI w'CCGG_w
Cuts at: 2589 4590 4988 5262 5409 5820 2589
Size: 2001 398 274 147 411 2926
Fragments arranged by size:
2926 2001 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2402 2432 2909 2939 3236
Size: 30 165 30 999 30 477 30 297
Cuts at: 3236 3266 1178
Size: 30 4069
Fragments arranged by size:
4069 999 477 297 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2273 2741 2744 2851 1186
Size: 1087 468 3 107 4492
Fragments arranged by size:
4492 1087 468 107 3
BseYI C'CCAG_C
Cuts at: 2035 2070 2188 2405 2889 3360 5360 5856 2035
Size: 35 118 217 484 471 2000 496 2336
Fragments arranged by size:
2336 2000 496 484 471 217 118 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5584
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2164 2206 3607 4368 5396 1540
Size: 275 186 163 42 1401 761 1028 2301
Fragments arranged by size:
2301 1401 1028 761 275 186 163 42
BsiHKAI G_wGCw'C
Cuts at: 2346 2659 2973 4218 4303 5374 2346
Size: 313 314 1245 85 1071 3129
Fragments arranged by size:
3129 1245 1071 314 313 85
BsmI GAATG_Cn'
Cuts at: 3668 3761 3668
Size: 93 6064
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2346 2415 2438 2659 2973 3368 3476
Size: 399 556 69 23 221 314 395 108
Cuts at: 3476 4218 4303 5374 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 556 399 395 314 221
108 85 69 23
BspEI T'CCGG_A
Cuts at: 5820 5820
Size: 6157
BspHI T'CATG_A
Cuts at: 4046 5776 4046
Size: 1730 4427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3512 844
Size: 2668 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3327 4044 1394
Size: 429 1504 717 3507
Fragments arranged by size:
3507 1504 717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2917 4652 4826 65
Size: 1183 1669 1735 174 1396
Fragments arranged by size:
1735 1669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2289 4801 4988 1700
Size: 110 479 2512 187 2869
Fragments arranged by size:
2869 2512 479 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6157
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6157
BssSI C'ACGA_G
Cuts at: 4217 5229 4217
Size: 1012 5145
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6157
BstBI TT'CG_AA
Cuts at: 4961 4961
Size: 6157
BstEII G'GTnAC_C
Cuts at: 1184 2807 1184
Size: 1623 4534
BstXI CCAn_nnnn'nTGG
Cuts at: 2803 2826 2803
Size: 23 6134
BstYI r'GATC_y
Cuts at: 1972 2521 2664 3249 3487 3854 4239 4256 5708
Size: 549 143 585 238 367 385 17 1452
Cuts at: 5708 5794 5806 6152 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 585 549 385 367 346 238
143 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2158 2280 2680 3144 3207 3244
Size: 552 939 154 122 400 464 63 37
Cuts at: 3244 3432 513
Size: 188 3238
Fragments arranged by size:
3238 939 552 464 400 188 154 122
63 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3386 487
Size: 683 56 2160 3258
Fragments arranged by size:
3258 2160 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 3673 4467 4487 2061
Size: 1612 794 20 3731
Fragments arranged by size:
3731 1612 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6157
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3189 3224 529
Size: 35 2625 35 3462
Fragments arranged by size:
3462 2625 35 35
DraI TTT'AAA
Cuts at: 3461 3461
Size: 6157
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2599 5164 809
Size: 507 1283 2565 1802
Fragments arranged by size:
2565 1802 1283 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2161 2203
Size: 54 1425 50 275 18 228 103 42
Cuts at: 2203 3033 3186 3210 3435 3604 4493 6122 8
Size: 830 153 24 225 169 889 1629 43
Fragments arranged by size:
1629 1425 889 830 275 228 225 169
153 103 54 50 43 42 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2161 2203 3604 1537
Size: 275 349 42 1401 4090
Fragments arranged by size:
4090 1401 349 275 42
EarI CTCTTCn'nnn_
Cuts at: 1353 4087 1353
Size: 2734 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2201 4744 5118 5264 387
Size: 1064 138 612 2543 374 146 1280
Fragments arranged by size:
2543 1280 1064 612 374 146 138
EcoICRI GAG'CTC
Cuts at: 2344 2971 2344
Size: 627 5530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2264 2276 2854 3077 4220 4817
Size: 393 533 99 12 578 223 1143 597
Cuts at: 4817 5604 5872 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1143 787 597 578 533 393 268
223 99 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2531 2531
Size: 6157
EcoO109I rG'GnC_Cy
Cuts at: 2671 2943 3414 2671
Size: 272 471 5414
Fragments arranged by size:
5414 471 272
EcoRI G'AATT_C
Cuts at: 3466 3844 3466
Size: 378 5779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 6157
FspI TGC'GCA
Cuts at: 4663 4663
Size: 6157
HaeII r_GCGC'y
Cuts at: 1678 2012 2129 2626 5304 1678
Size: 334 117 497 2678 2531
Fragments arranged by size:
2678 2531 497 334 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2402 2434 2437 2469 2513
Size: 32 135 32 830 32 3 32 44
Cuts at: 2513 2545 3186 3218 4798 4830 4872 4904 1373
Size: 32 641 32 1580 32 42 32 2626
Fragments arranged by size:
2626 1580 830 641 135 44 42 32
32 32 32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3501 3747 669
Size: 988 281 1563 246 3079
Fragments arranged by size:
3079 1563 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6157
HpaI GTT'AAC
Cuts at: 3747 3747
Size: 6157
KasI G'GCGC_C
Cuts at: 2008 2125 2622 2008
Size: 117 497 5543
Fragments arranged by size:
5543 497 117
KpnI G_GTAC'C
Cuts at: 3482 3482
Size: 6157
MfeI C'AATT_G
Cuts at: 3756 3756
Size: 6157
MluI A'CGCG_T
Cuts at: 6033 6033
Size: 6157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5271 5455 1305
Size: 3966 184 2007
Fragments arranged by size:
3966 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3188 3212 3437 10
Size: 54 1425 343 1356 24 225 2730
Fragments arranged by size:
2730 1425 1356 343 225 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2824 4115 4474 4633 518
Size: 648 1658 1291 359 159 2042
Fragments arranged by size:
2042 1658 1291 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2160 3360 3573 4250 5398 5643 1606
Size: 554 1200 213 677 1148 245 2120
Fragments arranged by size:
2120 1200 1148 677 554 245 213
NaeI GCC'GGC
Cuts at: 2291 2291
Size: 6157
NarI GG'CG_CC
Cuts at: 2009 2126 2623 2009
Size: 117 497 5543
Fragments arranged by size:
5543 497 117
NcoI C'CATG_G
Cuts at: 513 1065 2004 2280 3432 513
Size: 552 939 276 1152 3238
Fragments arranged by size:
3238 1152 939 552 276
NdeI CA'TA_TG
Cuts at: 387 2596 387
Size: 2209 3948
NgoMIV G'CCGG_C
Cuts at: 2289 2289
Size: 6157
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6157
NotI GC'GGCC_GC
Cuts at: 2203 3604 2203
Size: 1401 4756
NspI r_CATG'y
Cuts at: 1132 1229 2878 3094 3232 3515 5060 1132
Size: 97 1649 216 138 283 1545 2229
Fragments arranged by size:
2229 1649 1545 283 216 138 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6157
PciI A'CATG_T
Cuts at: 5056 5056
Size: 6157
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2335 2405 2674 1108
Size: 1227 70 269 4591
Fragments arranged by size:
4591 1227 269 70
PfoI T'CCnGG_A
Cuts at: 2334 2334
Size: 6157
PmeI GTTT'AAAC
Cuts at: 3461 3461
Size: 6157
PmlI CAC'GTG
Cuts at: 2356 2356
Size: 6157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2717 2749 2955 2987 4226 4258 5739 5771 2717
Size: 32 206 32 1239 32 1481 32 3103
Fragments arranged by size:
3103 1481 1239 206 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2943 2943
Size: 6157
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6157
PsiI TTA'TAA
Cuts at: 3727 3727
Size: 6157
PstI C_TGCA'G
Cuts at: 830 2022 2324 3278 3509 830
Size: 1192 302 954 231 3478
Fragments arranged by size:
3478 1192 954 302 231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6157
PvuII CAG'CTG
Cuts at: 3360 3360
Size: 6157
SacI G_AGCT'C
Cuts at: 2346 2973 2346
Size: 627 5530
SacII CC_GC'GG
Cuts at: 2161 2161
Size: 6157
SalI G'TCGA_C
Cuts at: 1936 3499 1936
Size: 1563 4594
SanDI GG'GwC_CC
Cuts at: 2943 2943
Size: 6157
SbfI CC_TGCA'GG
Cuts at: 3509 3509
Size: 6157
ScaI AGT'ACT
Cuts at: 4405 6117 4405
Size: 1712 4445
SfcI C'TryA_G
Cuts at: 826 1045 2018 2320 3073 3274 3505 4640 5321
Size: 219 973 302 753 201 231 1135 681
Cuts at: 5321 5512 826
Size: 191 1471
Fragments arranged by size:
1471 1135 973 753 681 302 231 219
201 191
SfoI GGC'GCC
Cuts at: 2010 2127 2624 2010
Size: 117 497 5543
Fragments arranged by size:
5543 497 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6157
SmaI CCC'GGG
Cuts at: 1872 2049 3484 1872
Size: 177 1435 4545
Fragments arranged by size:
4545 1435 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4261 5162 5424 820
Size: 197 926 2318 901 262 1553
Fragments arranged by size:
2318 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3948 493
Size: 3455 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6157
SphI G_CATG'C
Cuts at: 1132 3232 3515 1132
Size: 2100 283 3774
Fragments arranged by size:
3774 2100 283
SspI AAT'ATT
Cuts at: 5 52 4081 5
Size: 47 4029 2081
Fragments arranged by size:
4029 2081 47
StuI AGG'CCT
Cuts at: 1517 2096 2999 1517
Size: 579 903 4675
Fragments arranged by size:
4675 903 579
StyI C'CwwG_G
Cuts at: 513 1065 2004 2280 2391 3123 3281 3432 3596
Size: 552 939 276 111 732 158 151 164
Cuts at: 3596 513
Size: 3074
Fragments arranged by size:
3074 939 732 552 276 164 158 151
111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 4184 4201 4354 4539 4984 325
Size: 1255 1215 1389 17 153 185 445 1498
Fragments arranged by size:
1498 1389 1255 1215 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 4403 6115
Size: 274 80 33 51 1443 258 2168 1712
Cuts at: 6115 96
Size: 138
Fragments arranged by size:
2168 1712 1443 274 258 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2340 2359 2372 2380 2391 2412 2649 2681 2909
Size: 19 13 8 11 21 237 32 228
Cuts at: 2909 2941 3080 3112 3236 3268 6117 6149 2340
Size: 32 139 32 124 32 2849 32 2348
Fragments arranged by size:
2849 2348 237 228 139 124 32 32
32 32 32 21 19 13 11 8
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6157
XbaI T'CTAG_A
Cuts at: 3493 3493
Size: 6157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2876 3043 3445 2876
Size: 167 402 5588
Fragments arranged by size:
5588 402 167
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6157
XmaI C'CCGG_G
Cuts at: 1870 2047 3482 1870
Size: 177 1435 4545
Fragments arranged by size:
4545 1435 177
XmnI GAAnn'nnTTC
Cuts at: 4286 4286
Size: 6157
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3965 276
Size: 53 83 186 936 2431 2468
Fragments arranged by size:
2468 2431 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI
PsrI RsrII SapI SexAI SfiI SrfI SwaI