Restriction map for
pFN21AE5584
(Circular) (Six-base) MAPSORT of: pFN21AE5584.seq Check: 5360 from: 1 to: 5800
pFN21AE5584 5800 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2905 3110 3863 5247 5515 1632
Size: 1273 205 753 1384 268 1917
Fragments arranged by size:
1917 1384 1273 753 268 205
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 2888 4699 5676 1203
Size: 1685 1811 977 1327
Fragments arranged by size:
1811 1685 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 2437 4529 2437
Size: 2092 3708
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2948 1184
Size: 1764 4036
AlwNI CAG_nnn'CTG
Cuts at: 2198 2344 2673 3543 5115 2198
Size: 146 329 870 1572 2883
Fragments arranged by size:
2883 1572 870 329 146
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 2201 2970 3109 3335 3487 5596 1493
Size: 708 769 139 226 152 2109 1697
Fragments arranged by size:
2109 1697 769 708 226 152 139
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 3125 1870
Size: 73 1182 4545
Fragments arranged by size:
4545 1182 73
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3121 4576 618
Size: 325 217 627 1334 1455 1842
Fragments arranged by size:
1842 1455 1334 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2795 3119 1391
Size: 1404 324 4072
Fragments arranged by size:
4072 1404 324
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4829
BbvCI CC'TCA_GC
Cuts at: 2261 2261
Size: 5800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 2145 2307 1548
Size: 597 162 5041
Fragments arranged by size:
5041 597 162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4411 136
Size: 107 122 71 631 605 2739 1525
Fragments arranged by size:
2739 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 2357 3217 2357
Size: 860 4940
Bme1580I G_kGCm'C
Cuts at: 1790 3861 5017 1790
Size: 2071 1156 2573
Fragments arranged by size:
2573 2071 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2140 2186 3531
Size: 416 53 83 399 24 723 46 1345
Cuts at: 3531 4489 442
Size: 958 1753
Fragments arranged by size:
1753 1345 958 723 416 399 83 53
46 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2991 2991
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2122 2342 2450 2961 4460 1239
Size: 883 220 108 511 1499 2579
Fragments arranged by size:
2579 1499 883 511 220 108
Bpu10I CC'TnA_GC
Cuts at: 2261 2846 2261
Size: 585 5215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2902 3925 4790 5088 2902
Size: 1023 865 298 3614
Fragments arranged by size:
3614 1023 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2140 4463 882
Size: 617 641 2323 2219
Fragments arranged by size:
2323 2219 641 617
BsaAI yAC'GTr
Cuts at: 493 1204 2889 3591 493
Size: 711 1685 702 2702
Fragments arranged by size:
2702 1685 711 702
BsaBI GATnn'nnATC
Cuts at: 2312 2474 5794 2312
Size: 162 3320 2318
Fragments arranged by size:
3320 2318 162
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3607 3989 4624 275
Size: 53 83 186 936 2074 382 635 1451
Fragments arranged by size:
2074 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 1178
Size: 30 165 30 717 30 4828
Fragments arranged by size:
4828 717 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2354 1186
Size: 1012 156 4632
Fragments arranged by size:
4632 1012 156
BseYI C'CCAG_C
Cuts at: 2171 2452 3022 5003 5499 2171
Size: 281 570 1981 496 2472
Fragments arranged by size:
2472 1981 570 496 281
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2491 2697 2990 1163
Size: 573 582 173 206 293 3973
Fragments arranged by size:
3973 582 573 293 206 173
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 4011 5039 1540
Size: 275 186 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2388 2888 3861 3946 5017 2388
Size: 500 973 85 1071 3171
Fragments arranged by size:
3171 1071 973 500 85
BsmI GAATG_Cn'
Cuts at: 2629 3311 3404 2629
Size: 682 93 5025
Fragments arranged by size:
5025 682 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2388 2795 2888 3119 3861 3946 5017
Size: 399 598 407 93 231 742 85 1071
Cuts at: 5017 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 598 407 399 231 93
85
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 2478 3689 5419 2478
Size: 1211 1730 2859
Fragments arranged by size:
2859 1730 1211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2361 3093 3155 844
Size: 1517 732 62 3489
Fragments arranged by size:
3489 1517 732 62
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2182 4295 4469 65
Size: 1183 934 2113 174 1396
Fragments arranged by size:
2113 1396 1183 934 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 2320 2413 2320
Size: 93 5707
BstYI r'GATC_y
Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795
Size: 1158 367 385 17 1452 86 12 346
Cuts at: 5795 1972
Size: 1977
Fragments arranged by size:
1977 1452 1158 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2435 2435
Size: 5800
BtgI C'CryG_G
Cuts at: 513 1065 2283 2496 2601 513
Size: 552 1218 213 105 3712
Fragments arranged by size:
3712 1218 552 213 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 2184 3316 4110 4130 2184
Size: 1132 794 20 3854
Fragments arranged by size:
3854 1132 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 2022 3104 2022
Size: 1082 4718
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2500 4807 809
Size: 507 1184 2307 1802
Fragments arranged by size:
2307 1802 1184 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2415 2541
Size: 54 1425 50 275 18 450 135 126
Cuts at: 2541 2871 2958 3247 4136 5765 8
Size: 330 87 289 889 1629 43
Fragments arranged by size:
1629 1425 889 450 330 289 275 135
126 87 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 2643 2921 2960 3086 3730 1353
Size: 923 367 278 39 126 644 3423
Fragments arranged by size:
3423 923 644 367 278 126 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2342 2450 2905 2961 3110 3863
Size: 393 490 220 108 455 56 149 753
Cuts at: 3863 4460 5247 5515 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 753 597 490 455 393 268
220 149 108 56
EcoO109I rG'GnC_Cy
Cuts at: 2047 2410 2047
Size: 363 5437
EcoRI G'AATT_C
Cuts at: 2201 2970 3109 3487 2201
Size: 769 139 378 4514
Fragments arranged by size:
4514 769 378 139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2861 2861
Size: 5800
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 4947 1678
Size: 3269 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2423 2455 4441 4473 4515
Size: 32 135 32 851 32 1986 32 42
Cuts at: 4515 4547 1373
Size: 32 2626
Fragments arranged by size:
2626 1986 851 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4914 5098 1305
Size: 3609 184 2007
Fragments arranged by size:
3609 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2282 2417 2543 2873 2960
Size: 54 1425 343 450 135 126 330 87
Cuts at: 2960 10
Size: 2850
Fragments arranged by size:
2850 1425 450 343 330 135 126 87
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 2519 3758 4117 4276 518
Size: 648 1347 6 1239 359 159 2042
Fragments arranged by size:
2042 1347 1239 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2320 3216 3893 5041 5286 1606
Size: 714 896 677 1148 245 2120
Fragments arranged by size:
2120 1148 896 714 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2283 2496 513
Size: 552 1218 213 3817
Fragments arranged by size:
3817 1218 552 213
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2424 2590 2770 1108
Size: 1316 166 180 4138
Fragments arranged by size:
4138 1316 180 166
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PmlI CAC'GTG
Cuts at: 2889 2889
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2410 2410
Size: 5800
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PstI C_TGCA'G
Cuts at: 830 2708 3152 830
Size: 1878 444 3478
Fragments arranged by size:
3478 1878 444
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
PvuII CAG'CTG
Cuts at: 2320 2320
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 2704 3148 4283 4964 5155 826
Size: 219 1659 444 1135 681 191 1471
Fragments arranged by size:
1659 1471 1135 681 444 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2881 3904 4805 5067 820
Size: 197 926 938 1023 901 262 1553
Fragments arranged by size:
1553 1023 938 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2327 2879 1517
Size: 810 552 4438
Fragments arranged by size:
4438 810 552
StyI C'CwwG_G
Cuts at: 513 1065 2283 2406 2496 2874 3057 3239 513
Size: 552 1218 123 90 378 183 182 3074
Fragments arranged by size:
3074 1218 552 378 183 182 123 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3827 3844 3997 4182 4627 325
Size: 1255 2247 17 153 185 445 1498
Fragments arranged by size:
2247 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2085 2102 2346
Size: 274 80 33 51 1443 108 17 244
Cuts at: 2346 4046 5758 96
Size: 1700 1712 138
Fragments arranged by size:
1712 1700 1443 274 244 138 108 80
51 33 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2120 2152 5760 5792 2120
Size: 32 3608 32 2128
Fragments arranged by size:
3608 2128 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 2078 3136 2078
Size: 1058 4742
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI