Restriction map for
pFN21AE5583
(Circular) (Six-base) MAPSORT of: pFN21AE5583.seq Check: 8876 from: 1 to: 5791
pFN21AE5583 5791 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3601 278
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2727 3134 1937
Size: 790 407 4594
Fragments arranged by size:
4594 790 407
Acc65I G'GTAC_C
Cuts at: 3112 3112
Size: 5791
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2038 2366 3854 5238 5506 1632
Size: 406 328 1488 1384 268 1917
Fragments arranged by size:
1917 1488 1384 406 328 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 4690 5667 1203
Size: 3487 977 1327
Fragments arranged by size:
3487 1327 977
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 2387 2996 1166
Size: 1221 609 3961
Fragments arranged by size:
3961 1221 609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5791
AlwNI CAG_nnn'CTG
Cuts at: 2233 2401 2981 3534 5106 2233
Size: 168 580 553 1572 2918
Fragments arranged by size:
2918 1572 580 553 168
ApaLI G'TGCA_C
Cuts at: 3848 5004 3848
Size: 1156 4635
ApoI r'AATT_y
Cuts at: 1493 2657 3100 3326 3478 5587 1493
Size: 1164 443 226 152 2109 1697
Fragments arranged by size:
2109 1697 1164 443 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 3116 1870
Size: 73 1173 4545
Fragments arranged by size:
4545 1173 73
BamHI G'GATC_C
Cuts at: 3121 3488 3121
Size: 367 5424
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 3112 4567 618
Size: 325 217 627 335 990 1455 1842
Fragments arranged by size:
1842 1455 990 627 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2096 2199 2600 2846 3110 1391
Size: 705 103 401 246 264 4072
Fragments arranged by size:
4072 705 401 264 246 103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3982 4016 1903
Size: 34 2045 34 3678
Fragments arranged by size:
3678 2045 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3683 4893 3683
Size: 1210 4581
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4402 136
Size: 107 122 71 631 605 2730 1525
Fragments arranged by size:
2730 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5786 5786
Size: 5791
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 2394 3852 5008 1790
Size: 604 1458 1156 2573
Fragments arranged by size:
2573 1458 1156 604
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5791
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2079 2999 3522
Size: 416 53 83 399 24 662 920 523
Cuts at: 3522 4480 442
Size: 958 1753
Fragments arranged by size:
1753 958 920 662 523 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2170 2210 4451 1239
Size: 931 40 2241 2579
Fragments arranged by size:
2579 2241 931 40
Bpu10I CC'TnA_GC
Cuts at: 2174 2174
Size: 5791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3916 4781 5079 3916
Size: 865 298 4628
Fragments arranged by size:
4628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4454 882
Size: 617 2955 2219
Fragments arranged by size:
2955 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3582 493
Size: 711 2378 2702
Fragments arranged by size:
2702 2378 711
BsaBI GATnn'nnATC
Cuts at: 5785 5785
Size: 5791
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3598 3980 4615 275
Size: 53 83 186 936 2065 382 635 1451
Fragments arranged by size:
2065 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4224 4622 4896 5043 5454 4224
Size: 398 274 147 411 4561
Fragments arranged by size:
4561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5566
Fragments arranged by size:
5566 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2368 1186
Size: 1182 4609
BseYI C'CCAG_C
Cuts at: 2220 2275 4994 5490 2220
Size: 55 2719 496 2521
Fragments arranged by size:
2719 2521 496 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 2628 2770 1163
Size: 573 570 322 142 4184
Fragments arranged by size:
4184 573 570 322 142
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4002 5030 1540
Size: 275 186 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2199 2671 2846 3852 3937 5008 2148
Size: 51 472 175 1006 85 1071 2931
Fragments arranged by size:
2931 1071 1006 472 175 85 51
BsmI GAATG_Cn'
Cuts at: 3302 3395 3302
Size: 93 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2148 2199 2394 2600 2671 2846
Size: 399 306 52 51 195 206 71 175
Cuts at: 2846 3110 3852 3937 5008 1391
Size: 264 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 264 206 195
175 85 71 52 51
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 3680 5410 3680
Size: 1730 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2847 2943 3146 844
Size: 2003 96 203 3489
Fragments arranged by size:
3489 2003 203 96
BsrBI CCG'CTC
Cuts at: 1394 1823 3678 1394
Size: 429 1855 3507
Fragments arranged by size:
3507 1855 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4286 4460 65
Size: 1183 3038 174 1396
Fragments arranged by size:
3038 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4435 4622 1700
Size: 110 2625 187 2869
Fragments arranged by size:
2869 2625 187 110
BsrGI T'GTAC_A
Cuts at: 96 2259 96
Size: 2163 3628
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5791
BssSI C'ACGA_G
Cuts at: 3851 4863 3851
Size: 1012 4779
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5791
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2275 2275
Size: 5791
BstYI r'GATC_y
Cuts at: 1972 2745 3121 3488 3873 3890 5342 5428 5440
Size: 773 376 367 385 17 1452 86 12
Cuts at: 5440 5786 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 773 385 376 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2068 2571 2068
Size: 503 5288
BtgI C'CryG_G
Cuts at: 513 1065 2382 2508 513
Size: 552 1317 126 3796
Fragments arranged by size:
3796 1317 552 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2122 2485 487
Size: 683 56 896 363 3793
Fragments arranged by size:
3793 896 683 363 56
BtsI GCAGTG_nn'
Cuts at: 2892 3307 4101 4121 2892
Size: 415 794 20 4562
Fragments arranged by size:
4562 794 415 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DraIII CAC_nnn'GTG
Cuts at: 2741 2741
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4798 809
Size: 507 3482 1802
Fragments arranged by size:
3482 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2415 3238 4127
Size: 54 1425 50 275 18 585 823 889
Cuts at: 4127 5756 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 823 585 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2243 2342 2759 2912 3721 1353
Size: 890 99 417 153 809 3423
Fragments arranged by size:
3423 890 809 417 153 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4378 4752 4898 387
Size: 1064 138 2789 374 146 1280
Fragments arranged by size:
2789 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2197 2844 2197
Size: 647 5144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2170 2210 2366 3854 4451 5238
Size: 393 406 132 40 156 1488 597 787
Cuts at: 5238 5506 1239
Size: 268 1524
Fragments arranged by size:
1524 1488 787 597 406 393 268 156
132 40
EcoRI G'AATT_C
Cuts at: 3100 3478 3100
Size: 378 5413
FspI TGC'GCA
Cuts at: 4297 4297
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 4938 1678
Size: 3260 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4432 4464 4506 4538 1373
Size: 32 135 32 2860 32 42 32 2626
Fragments arranged by size:
2860 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3135 3381 669
Size: 988 281 1197 246 3079
Fragments arranged by size:
3079 1197 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3054 748
Size: 2306 3485
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KpnI G_GTAC'C
Cuts at: 3116 3116
Size: 5791
MfeI C'AATT_G
Cuts at: 3390 3390
Size: 5791
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4905 5089 1305
Size: 3600 184 2007
Fragments arranged by size:
3600 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2417 10
Size: 54 1425 343 585 3384
Fragments arranged by size:
3384 1425 585 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2310 2330 2387 2996 3749 4108 4267
Size: 648 1144 20 57 609 753 359 159
Cuts at: 4267 518
Size: 2042
Fragments arranged by size:
2042 1144 753 648 609 359 159 57
20
MspA1I CmG'CkG
Cuts at: 1606 3207 3884 5032 5277 1606
Size: 1601 677 1148 245 2120
Fragments arranged by size:
2120 1601 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2508 513
Size: 552 1443 3796
Fragments arranged by size:
3796 1443 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 2260 3149 4694 1132
Size: 97 1031 889 1545 2229
Fragments arranged by size:
2229 1545 1031 889 97
PasI CC'CwG_GG
Cuts at: 1348 2851 1348
Size: 1503 4288
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 2401 1108
Size: 1077 216 4498
Fragments arranged by size:
4498 1077 216
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PstI C_TGCA'G
Cuts at: 830 3143 830
Size: 2313 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
SacI G_AGCT'C
Cuts at: 2199 2846 2199
Size: 647 5144
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SapI GCTCTTCn'nnn_
Cuts at: 2243 2342 2759 2243
Size: 99 417 5275
Fragments arranged by size:
5275 417 99
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SexAI A'CCwGG_T
Cuts at: 2283 2283
Size: 5791
SfcI C'TryA_G
Cuts at: 826 1045 2602 3139 4274 4955 5146 826
Size: 219 1557 537 1135 681 191 1471
Fragments arranged by size:
1557 1471 1135 681 537 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 3118 1872
Size: 1246 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3895 4796 5058 820
Size: 197 926 1952 901 262 1553
Fragments arranged by size:
1952 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 3149 1132
Size: 2017 3774
SspI AAT'ATT
Cuts at: 5 52 3715 5
Size: 47 3663 2081
Fragments arranged by size:
3663 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5791
StyI C'CwwG_G
Cuts at: 513 1065 2179 2508 3230 513
Size: 552 1114 329 722 3074
Fragments arranged by size:
3074 1114 722 552 329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2344 2719 3818 3835 3988 4173 4618
Size: 1255 764 375 1099 17 153 185 445
Cuts at: 4618 325
Size: 1498
Fragments arranged by size:
1498 1255 1099 764 445 375 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 2334 3024
Size: 274 80 33 51 1443 282 75 690
Cuts at: 3024 4037 5749 96
Size: 1013 1712 138
Fragments arranged by size:
1712 1443 1013 690 282 274 138 80
75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5751 5783 5751
Size: 32 5759
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5791
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 3116 1870
Size: 1246 4545
XmnI GAAnn'nnTTC
Cuts at: 3920 3920
Size: 5791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3599 276
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI
SwaI XcmI