Restriction map for
pFN21AE5582
(Circular) (Six-base) MAPSORT of: pFN21AE5582.seq Check: 9010 from: 1 to: 5782
pFN21AE5582 5782 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2541 3125 1937
Size: 604 584 4594
Fragments arranged by size:
4594 604 584
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 2183 2819 2835 3845 5229 5497 1632
Size: 531 20 636 16 1010 1384 268 1917
Fragments arranged by size:
1917 1384 1010 636 531 268 20 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 2840 4681 5658 1203
Size: 1637 1841 977 1327
Fragments arranged by size:
1841 1637 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 2960 1166
Size: 1794 3988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 3525 5097 3525
Size: 1572 4210
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 2245 3091 3317 3469 5578 1493
Size: 752 846 226 152 2109 1697
Fragments arranged by size:
2109 1697 846 752 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 3107 1870
Size: 73 1164 4545
Fragments arranged by size:
4545 1164 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3103 4558 618
Size: 325 217 627 1316 1455 1842
Fragments arranged by size:
1842 1455 1316 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2735 2851 3101 1391
Size: 1344 116 250 4072
Fragments arranged by size:
4072 1344 250 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2313 2710 2824 928
Size: 971 414 397 114 3886
Fragments arranged by size:
3886 971 414 397 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BfrBI ATG'CAT
Cuts at: 2098 2098
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4393 136
Size: 107 122 71 631 605 2721 1525
Fragments arranged by size:
2721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 3843 4999 1790
Size: 2053 1156 2573
Fragments arranged by size:
2573 2053 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2880 4442 1239
Size: 1641 1562 2579
Fragments arranged by size:
2579 1641 1562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2065 3907 4772 5070 2065
Size: 1842 865 298 2777
Fragments arranged by size:
2777 1842 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2896 4445 882
Size: 617 1397 1549 2219
Fragments arranged by size:
2219 1549 1397 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5557
Fragments arranged by size:
5557 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2658 2836 1186
Size: 1472 178 4132
Fragments arranged by size:
4132 1472 178
BseYI C'CCAG_C
Cuts at: 2280 4985 5481 2280
Size: 2705 496 2581
Fragments arranged by size:
2705 2581 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 3843 3928 4999 2030
Size: 1813 85 1071 2813
Fragments arranged by size:
2813 1813 1071 85
BsmI GAATG_Cn'
Cuts at: 2459 3293 3386 2459
Size: 834 93 4855
Fragments arranged by size:
4855 834 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2735 2851 3101 3843 3928 4999
Size: 399 240 705 116 250 742 85 1071
Cuts at: 4999 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 705 399 250 240 116
85
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3137 844
Size: 2293 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2478 4277 4451 65
Size: 1183 1230 1799 174 1396
Fragments arranged by size:
1799 1396 1230 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2858 2858
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777
Size: 1140 367 385 17 1452 86 12 346
Cuts at: 5777 1972
Size: 1977
Fragments arranged by size:
1977 1452 1140 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 3298 4092 4112 3298
Size: 794 20 4968
Fragments arranged by size:
4968 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2790 3229 4118
Size: 54 1425 50 275 18 960 439 889
Cuts at: 4118 5747 8
Size: 1629 43
Fragments arranged by size:
1629 1425 960 889 439 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2234 2894 3712 1353
Size: 881 660 818 3423
Fragments arranged by size:
3423 881 818 660
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 2183 2819 2835 2880 3845 4442
Size: 393 531 20 636 16 45 965 597
Cuts at: 4442 5229 5497 1239
Size: 787 268 1524
Fragments arranged by size:
1524 965 787 636 597 531 393 268
45 20 16
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
EcoRV GAT'ATC
Cuts at: 2662 2662
Size: 5782
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 4929 1678
Size: 3251 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373
Size: 32 135 32 2851 32 42 32 2626
Fragments arranged by size:
2851 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2035 3126 3372 669
Size: 988 281 97 1091 246 3079
Fragments arranged by size:
3079 1091 988 281 246 97
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 2035 3372 2035
Size: 1337 4445
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4896 5080 1305
Size: 3591 184 2007
Fragments arranged by size:
3591 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2792 10
Size: 54 1425 343 960 3000
Fragments arranged by size:
3000 1425 960 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2897 2939 2960 3740 4099 4258
Size: 648 1071 660 42 21 780 359 159
Cuts at: 4258 518
Size: 2042
Fragments arranged by size:
2042 1071 780 660 648 359 159 42
21
MspA1I CmG'CkG
Cuts at: 1606 3198 3875 5023 5268 1606
Size: 1592 677 1148 245 2120
Fragments arranged by size:
2120 1592 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5230
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NsiI A_TGCA'T
Cuts at: 2100 2100
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2530 2548 2588 2844 3140 4685 1132
Size: 97 1301 18 40 256 296 1545 2229
Fragments arranged by size:
2229 1545 1301 296 256 97 40 18
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 2840 4681 2840
Size: 1841 3941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2893 2925 3851 3883 5364 5396 2893
Size: 32 926 32 1481 32 3279
Fragments arranged by size:
3279 1481 926 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2193 3134 830
Size: 1363 941 3478
Fragments arranged by size:
3478 1363 941
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2189 2989 3130 4265 4946 5137 826
Size: 219 1144 800 141 1135 681 191 1471
Fragments arranged by size:
1471 1144 1135 800 681 219 191 141
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2044 3886 4787 5049 820
Size: 197 926 101 1842 901 262 1553
Fragments arranged by size:
1842 1553 926 901 262 197 101
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 2949 3706 5
Size: 47 2897 757 2081
Fragments arranged by size:
2897 2081 757 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2404 3221 513
Size: 552 1339 817 3074
Fragments arranged by size:
3074 1339 817 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2548 3809 3826 3979 4164 4609 325
Size: 1255 968 1261 17 153 185 445 1498
Fragments arranged by size:
1498 1261 1255 968 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2158 2298 4028
Size: 274 80 33 51 1443 181 140 1730
Cuts at: 4028 5740 96
Size: 1712 138
Fragments arranged by size:
1730 1712 1443 274 181 140 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 2116 3118 2116
Size: 1002 4780
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 2168 2457 3911 2168
Size: 289 1454 4039
Fragments arranged by size:
4039 1454 289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI