Restriction map for
pFN21AE5579
(Circular) (Six-base) MAPSORT of: pFN21AE5579.seq Check: 1537 from: 1 to: 5770
pFN21AE5579 5770 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3018 3113 1937
Size: 1081 95 4594
Fragments arranged by size:
4594 1081 95
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2184 2814 3833 5217 5485 1632
Size: 552 630 1019 1384 268 1917
Fragments arranged by size:
1917 1384 1019 630 552 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 2219 4601 2219
Size: 2382 3388
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 3513 5085 3513
Size: 1572 4198
ApaI G_GGCC'C
Cuts at: 2701 2701
Size: 5770
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 2378 2404 2452 3046 3079 3305 3457 5566
Size: 885 26 48 594 33 226 152 2109
Cuts at: 5566 1493
Size: 1697
Fragments arranged by size:
2109 1697 885 594 226 152 48 33
26
AseI AT'TA_AT
Cuts at: 160 1032 2429 4324 160
Size: 872 1397 1895 1606
Fragments arranged by size:
1895 1606 1397 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 3095 1870
Size: 73 1152 4545
Fragments arranged by size:
4545 1152 73
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3091 4546 618
Size: 325 217 627 1304 1455 1842
Fragments arranged by size:
1842 1455 1304 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2701 3089 1391
Size: 1310 388 4072
Fragments arranged by size:
4072 1310 388
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 928
Size: 971 963 3836
Fragments arranged by size:
3836 971 963
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2557 3662 4872 2557
Size: 1105 1210 3455
Fragments arranged by size:
3455 1210 1105
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4381 136
Size: 107 122 71 631 605 2709 1525
Fragments arranged by size:
2709 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2333 2797 2908 5765 2333
Size: 464 111 2857 2338
Fragments arranged by size:
2857 2338 464 111
BlpI GC'TnA_GC
Cuts at: 2049 3187 2049
Size: 1138 4632
Bme1580I G_kGCm'C
Cuts at: 1790 2701 3831 4987 1790
Size: 911 1130 1156 2573
Fragments arranged by size:
2573 1156 1130 911
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 4459 442
Size: 416 53 83 399 24 2084 958 1753
Fragments arranged by size:
2084 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4430 1239
Size: 3191 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3895 4760 5058 3895
Size: 865 298 4607
Fragments arranged by size:
4607 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4433 882
Size: 617 2934 2219
Fragments arranged by size:
2934 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 2739 5764 2739
Size: 3025 2745
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2219 2780 4203 4601 4875 5022 5433 2219
Size: 561 1423 398 274 147 411 2556
Fragments arranged by size:
2556 1423 561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5545
Fragments arranged by size:
5545 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2172 2696 1186
Size: 986 524 4260
Fragments arranged by size:
4260 986 524
BseYI C'CCAG_C
Cuts at: 4973 5469 4973
Size: 496 5274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3220 3981 5009 1540
Size: 275 186 1219 761 1028 2301
Fragments arranged by size:
2301 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2050 3831 3916 4987 2050
Size: 1781 85 1071 2833
Fragments arranged by size:
2833 1781 1071 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2701 3089 3831 3916 4987 1391
Size: 399 260 651 388 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 399 388 260 85
BspEI T'CCGG_A
Cuts at: 2780 5433 2780
Size: 2653 3117
BspHI T'CATG_A
Cuts at: 2020 3659 5389 2020
Size: 1639 1730 2401
Fragments arranged by size:
2401 1730 1639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3125 844
Size: 2281 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3657 1394
Size: 429 1834 3507
Fragments arranged by size:
3507 1834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2254 4265 4439 65
Size: 1183 1006 2011 174 1396
Fragments arranged by size:
2011 1396 1183 1006 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2219 4414 4601 1700
Size: 110 409 2195 187 2869
Fragments arranged by size:
2869 2195 409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 2333 2797 2908 3100 3467 3852 3869 5321
Size: 361 464 111 192 367 385 17 1452
Cuts at: 5321 5407 5419 5765 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 464 385 367 361 346 192
111 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2775 487
Size: 683 56 1549 3482
Fragments arranged by size:
3482 1549 683 56
BtsI GCAGTG_nn'
Cuts at: 3286 4080 4100 3286
Size: 794 20 4956
Fragments arranged by size:
4956 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3217 4106 5735
Size: 54 1425 50 275 18 1387 889 1629
Cuts at: 5735 8
Size: 43
Fragments arranged by size:
1629 1425 1387 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2166 2550 3700 1353
Size: 774 39 384 1150 3423
Fragments arranged by size:
3423 1150 774 384 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2184 2814 3833 4430 5217 5485 1239
Size: 393 552 630 1019 597 787 268 1524
Fragments arranged by size:
1524 1019 787 630 597 552 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2697 2698 2697
Size: 1 5769
EcoRI G'AATT_C
Cuts at: 2404 3079 3457 2404
Size: 675 378 4717
Fragments arranged by size:
4717 675 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2195 2195
Size: 5770
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4411 4443 4485 4517 1373
Size: 32 135 32 2839 32 42 32 2626
Fragments arranged by size:
2839 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3019 3114 3360 669
Size: 988 281 1081 95 246 3079
Fragments arranged by size:
3079 1081 988 281 246 95
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 2109 2964 3369 2109
Size: 855 405 4510
Fragments arranged by size:
4510 855 405
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4884 5068 1305
Size: 3579 184 2007
Fragments arranged by size:
3579 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3948
Fragments arranged by size:
3948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3728 4087 4246 518
Size: 648 2562 359 159 2042
Fragments arranged by size:
2562 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3014 3186 3863 5011 5256 1606
Size: 1408 172 677 1148 245 2120
Fragments arranged by size:
2120 1408 1148 677 245 172
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2445 2770 3128 4673 1132
Size: 97 1216 325 358 1545 2229
Fragments arranged by size:
2229 1545 1216 358 325 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5770
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 2352 3340 2352
Size: 988 4782
PspOMI G'GGCC_C
Cuts at: 2697 2697
Size: 5770
PstI C_TGCA'G
Cuts at: 830 2541 3122 830
Size: 1711 581 3478
Fragments arranged by size:
3478 1711 581
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3017 3112 1936
Size: 1081 95 4594
Fragments arranged by size:
4594 1081 95
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 2752 4018 5730 2752
Size: 1266 1712 2792
Fragments arranged by size:
2792 1712 1266
SfcI C'TryA_G
Cuts at: 826 1045 2537 3118 4253 4934 5125 826
Size: 219 1492 581 1135 681 191 1471
Fragments arranged by size:
1492 1471 1135 681 581 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3874 4775 5037 820
Size: 197 926 1931 901 262 1553
Fragments arranged by size:
1931 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 2770 3128 1132
Size: 1638 358 3774
Fragments arranged by size:
3774 1638 358
SspI AAT'ATT
Cuts at: 5 52 2084 3694 5
Size: 47 2032 1610 2081
Fragments arranged by size:
2081 2032 1610 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2004 3209 513
Size: 552 939 1205 3074
Fragments arranged by size:
3074 1205 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3797 3814 3967 4152 4597 325
Size: 1255 2217 17 153 185 445 1498
Fragments arranged by size:
2217 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2750 3004 4016
Size: 274 80 33 51 1443 773 254 1012
Cuts at: 4016 5728 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1012 773 274 254 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI