Restriction map for
pFN21AE5578
(Circular) (Six-base) MAPSORT of: pFN21AE5578.seq Check: 2808 from: 1 to: 6079
pFN21AE5578 6079 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2805 2805
Size: 6079
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3422 1937
Size: 1485 4594
Acc65I G'GTAC_C
Cuts at: 2958 3400 2958
Size: 442 5637
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2984 3350 4142 5526 5794 1632
Size: 1352 366 792 1384 268 1917
Fragments arranged by size:
1917 1384 1352 792 366 268
AfeI AGC'GCT
Cuts at: 2399 2903 2399
Size: 504 5575
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 2478 4978 5955 1203
Size: 1275 2500 977 1327
Fragments arranged by size:
2500 1327 1275 977
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 3077 1166
Size: 1911 4168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3234 1184
Size: 2050 4029
AlwNI CAG_nnn'CTG
Cuts at: 2264 2389 2512 2766 3822 5394 2264
Size: 125 123 254 1056 1572 2949
Fragments arranged by size:
2949 1572 1056 254 125 123
ApaI G_GGCC'C
Cuts at: 2050 2050
Size: 6079
ApaLI G'TGCA_C
Cuts at: 3255 3323 4136 5292 3255
Size: 68 813 1156 4042
Fragments arranged by size:
4042 1156 813 68
ApoI r'AATT_y
Cuts at: 1493 3388 3614 3766 5875 1493
Size: 1895 226 152 2109 1697
Fragments arranged by size:
2109 1895 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 2863 3189 3206 3404 1870
Size: 73 920 326 17 198 4545
Fragments arranged by size:
4545 920 326 198 73 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2336 2369 2336
Size: 33 6046
BamHI G'GATC_C
Cuts at: 2737 3409 3776 2737
Size: 672 367 5040
Fragments arranged by size:
5040 672 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 2636 2805 2958 3043
Size: 325 217 627 245 604 169 153 85
Cuts at: 3043 3135 3400 4855 618
Size: 92 265 1455 1842
Fragments arranged by size:
1842 1455 627 604 325 265 245 217
169 153 92 85
BanII G_rGCy'C
Cuts at: 1391 2050 3398 1391
Size: 659 1348 4072
Fragments arranged by size:
4072 1348 659
BbeI G_GCGC'C
Cuts at: 2640 3139 2640
Size: 499 5580
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3356 928
Size: 971 1457 3651
Fragments arranged by size:
3651 1457 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2288 2322 2383 2417 2700 2734 4270
Size: 34 351 34 61 34 283 34 1536
Cuts at: 4270 4304 1903
Size: 34 3678
Fragments arranged by size:
3678 1536 351 283 61 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 3003 1548
Size: 1455 4624
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 3133 4690
Size: 107 122 71 631 605 382 1079 1557
Cuts at: 4690 136
Size: 1525
Fragments arranged by size:
1557 1525 1079 631 605 382 122 107
71
BglII A'GATC_T
Cuts at: 2466 6074 2466
Size: 3608 2471
BlpI GC'TnA_GC
Cuts at: 3496 3496
Size: 6079
Bme1580I G_kGCm'C
Cuts at: 1790 2050 3237 3259 3327 4140 5296 1790
Size: 260 1187 22 68 813 1156 2573
Fragments arranged by size:
2573 1187 1156 813 260 68 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2339 3810 4768
Size: 416 53 83 399 24 922 1471 958
Cuts at: 4768 442
Size: 1753
Fragments arranged by size:
1753 1471 958 922 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2743 4739 1239
Size: 1504 1996 2579
Fragments arranged by size:
2579 1996 1504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4204 5069 5367 4204
Size: 865 298 4916
Fragments arranged by size:
4916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2693 3320 4742 882
Size: 617 1194 627 1422 2219
Fragments arranged by size:
2219 1422 1194 627 617
BsaAI yAC'GTr
Cuts at: 493 1204 3870 493
Size: 711 2666 2702
Fragments arranged by size:
2702 2666 711
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2637 2972 3136 3886
Size: 53 83 186 936 1104 335 164 750
Cuts at: 3886 4268 4903 275
Size: 382 635 1451
Fragments arranged by size:
1451 1104 936 750 635 382 335 186
164 83 53
BsaWI w'CCGG_w
Cuts at: 2486 4512 4910 5184 5331 5742 2486
Size: 2026 398 274 147 411 2823
Fragments arranged by size:
2823 2026 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2468 2498 1178
Size: 30 165 30 1065 30 4759
Fragments arranged by size:
4759 1065 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2834 3291 1186
Size: 838 810 457 3974
Fragments arranged by size:
3974 838 810 457
BseYI C'CCAG_C
Cuts at: 3236 5282 5778 3236
Size: 2046 496 3537
Fragments arranged by size:
3537 2046 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2158 2899 2918 3076 3240 1163
Size: 573 422 741 19 158 164 4002
Fragments arranged by size:
4002 741 573 422 164 158 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2410 3529 4290 5318 1540
Size: 275 186 409 1119 761 1028 2301
Fragments arranged by size:
2301 1119 1028 761 409 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 2439 3259 3327 4140 4225 5296 2016
Size: 423 820 68 813 85 1071 2799
Fragments arranged by size:
2799 1071 820 813 423 85 68
BsmI GAATG_Cn'
Cuts at: 2311 3012 3590 3683 2311
Size: 701 578 93 4707
Fragments arranged by size:
4707 701 578 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2866 2866
Size: 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2050 2439 3237 3259 3327 3398
Size: 399 226 34 389 798 22 68 71
Cuts at: 3398 4140 4225 5296 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 798 742 399 389 226 85
71 68 34 22
BspEI T'CCGG_A
Cuts at: 2486 5742 2486
Size: 3256 2823
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2397 2805 3434 844
Size: 1553 408 629 3489
Fragments arranged by size:
3489 1553 629 408
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2088 4574 4748 65
Size: 1183 840 2486 174 1396
Fragments arranged by size:
2486 1396 1183 840 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2200 4723 4910 1700
Size: 110 390 2523 187 2869
Fragments arranged by size:
2869 2523 390 187 110
BsrGI T'GTAC_A
Cuts at: 96 2451 2598 96
Size: 2355 147 3577
Fragments arranged by size:
3577 2355 147
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 2666 4139 5151 2666
Size: 1473 1012 3594
Fragments arranged by size:
3594 1473 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 2466 2737 2821 3409 3776 4161 4178 5630
Size: 494 271 84 588 367 385 17 1452
Cuts at: 5630 5716 5728 6074 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 588 494 385 367 346 271
86 84 17 12
Bsu36I CC'TnA_GG
Cuts at: 3048 3048
Size: 6079
BtgI C'CryG_G
Cuts at: 513 1065 2004 2745 2828 3104 513
Size: 552 939 741 83 276 3488
Fragments arranged by size:
3488 939 741 552 276 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2524 3079 487
Size: 683 56 1298 555 3487
Fragments arranged by size:
3487 1298 683 555 56
BtsI GCAGTG_nn'
Cuts at: 2242 3595 4389 4409 2242
Size: 1353 794 20 3912
Fragments arranged by size:
3912 1353 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DraIII CAC_nnn'GTG
Cuts at: 2248 2671 2812 2248
Size: 423 141 5515
Fragments arranged by size:
5515 423 141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2084 5086 809
Size: 507 768 3002 1802
Fragments arranged by size:
3002 1802 768 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2198 2239 2407
Size: 54 1425 50 275 18 368 41 168
Cuts at: 2407 2586 2910 3526 4415 6044 8
Size: 179 324 616 889 1629 43
Fragments arranged by size:
1629 1425 889 616 368 324 275 179
168 54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2407 3526 1537
Size: 275 595 1119 4090
Fragments arranged by size:
4090 1119 595 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 2318 2696 2912 4009 1353
Size: 869 96 378 216 1097 3423
Fragments arranged by size:
3423 1097 869 378 216 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2408 4666 5040 5186 387
Size: 1064 138 819 2258 374 146 1280
Fragments arranged by size:
2258 1280 1064 819 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2743 2984 3350 4142 4739 5526 5794
Size: 393 1111 241 366 792 597 787 268
Cuts at: 5794 1239
Size: 1524
Fragments arranged by size:
1524 1111 792 787 597 393 366 268
241
EcoO109I rG'GnC_Cy
Cuts at: 2046 2056 2208 3158 2046
Size: 10 152 950 4967
Fragments arranged by size:
4967 950 152 10
EcoRI G'AATT_C
Cuts at: 3388 3766 3388
Size: 378 5701
FspI TGC'GCA
Cuts at: 2393 4585 2393
Size: 2192 3887
HaeII r_GCGC'y
Cuts at: 1678 2271 2401 2640 2905 3139 5226 1678
Size: 593 130 239 265 234 2087 2531
Fragments arranged by size:
2531 2087 593 265 239 234 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2053 2085 2468 2500 2806
Size: 32 135 32 481 32 383 32 306
Cuts at: 2806 2838 2841 2873 4720 4752 4794 4826 1373
Size: 32 3 32 1847 32 42 32 2626
Fragments arranged by size:
2626 1847 481 383 306 135 42 32
32 32 32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3423 3669 669
Size: 988 281 1485 246 3079
Fragments arranged by size:
3079 1485 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6079
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KasI G'GCGC_C
Cuts at: 2636 3135 2636
Size: 499 5580
KpnI G_GTAC'C
Cuts at: 2962 3404 2962
Size: 442 5637
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3114 5193 5377 1305
Size: 1809 2079 184 2007
Fragments arranged by size:
2079 2007 1809 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2241 10
Size: 54 1425 343 409 3848
Fragments arranged by size:
3848 1425 409 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2375 2558 2648 2990 3077 4037 4396
Size: 648 1209 183 90 342 87 960 359
Cuts at: 4396 4555 518
Size: 159 2042
Fragments arranged by size:
2042 1209 960 648 359 342 183 159
90 87
MspA1I CmG'CkG
Cuts at: 1606 2363 3106 3214 3495 4172 5320 5565 1606
Size: 757 743 108 281 677 1148 245 2120
Fragments arranged by size:
2120 1148 757 743 677 281 245 108
NarI GG'CG_CC
Cuts at: 2637 3136 2637
Size: 499 5580
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 2407 3526 2407
Size: 1119 4960
NspI r_CATG'y
Cuts at: 1132 1229 2482 3437 4982 1132
Size: 97 1253 955 1545 2229
Fragments arranged by size:
2229 1545 1253 955 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 2478 4978 2478
Size: 2500 3579
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2248 2671 3359 1108
Size: 1140 423 688 3828
Fragments arranged by size:
3828 1140 688 423
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2621 1613
Size: 1008 5071
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PspOMI G'GGCC_C
Cuts at: 2046 2046
Size: 6079
PstI C_TGCA'G
Cuts at: 830 3431 830
Size: 2601 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 3214 3214
Size: 6079
SacII CC_GC'GG
Cuts at: 3107 3107
Size: 6079
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SapI GCTCTTCn'nnn_
Cuts at: 2318 2912 2318
Size: 594 5485
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SexAI A'CCwGG_T
Cuts at: 2886 2886
Size: 6079
SfcI C'TryA_G
Cuts at: 826 1045 3427 4562 5243 5434 826
Size: 219 2382 1135 681 191 1471
Fragments arranged by size:
2382 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2054 2054
Size: 6079
SfoI GGC'GCC
Cuts at: 2638 3137 2638
Size: 499 5580
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4183 5084 5346 820
Size: 197 926 2240 901 262 1553
Fragments arranged by size:
2240 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6079
StyI C'CwwG_G
Cuts at: 513 1065 2004 3518 513
Size: 552 939 1514 3074
Fragments arranged by size:
3074 1514 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4106 4123 4276 4461 4906 325
Size: 1255 2526 17 153 185 445 1498
Fragments arranged by size:
2526 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2451 2598 4325
Size: 274 80 33 51 1443 474 147 1727
Cuts at: 4325 6037 96
Size: 1712 138
Fragments arranged by size:
1727 1712 1443 474 274 147 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2813 2845 6039 6071 2813
Size: 32 3194 32 2821
Fragments arranged by size:
3194 2821 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2728 2888 1316
Size: 1412 160 4507
Fragments arranged by size:
4507 1412 160
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2470 2983 2470
Size: 513 5566
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 4208 4208
Size: 6079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BstXI
BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SrfI SwaI