Restriction map for
pFN21AE5570
(Circular) (Six-base) MAPSORT of: pFN21AE5570.seq Check: 169 from: 1 to: 6070
pFN21AE5570 6070 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3880 278
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3413 1937
Size: 1476 4594
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 2418 4197 4570 1563
Size: 855 1779 373 3063
Fragments arranged by size:
3063 1779 855 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4133 5517 5785 1632
Size: 2501 1384 268 1917
Fragments arranged by size:
2501 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 2493 4969 5946 1203
Size: 1290 2476 977 1327
Fragments arranged by size:
2476 1327 1290 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 2407 4799 2407
Size: 2392 3678
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6070
AlwNI CAG_nnn'CTG
Cuts at: 3185 3813 5385 3185
Size: 628 1572 3870
Fragments arranged by size:
3870 1572 628
ApaLI G'TGCA_C
Cuts at: 4127 5283 4127
Size: 1156 4914
ApoI r'AATT_y
Cuts at: 1493 2039 3229 3379 3605 3757 5866 1493
Size: 546 1190 150 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 546 226 152 150
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 3395 1870
Size: 73 1452 4545
Fragments arranged by size:
4545 1452 73
BamHI G'GATC_C
Cuts at: 3400 3767 3400
Size: 367 5703
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 2462 3391 4846 618
Size: 325 217 627 231 444 929 1455 1842
Fragments arranged by size:
1842 1455 929 627 444 325 231 217
BanII G_rGCy'C
Cuts at: 1391 3389 1391
Size: 1998 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5099
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4261 4295 1903
Size: 34 2324 34 3678
Fragments arranged by size:
3678 2324 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3962 5172 3962
Size: 1210 4860
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6070
BfrBI ATG'CAT
Cuts at: 2359 3193 2359
Size: 834 5236
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4681 136
Size: 107 122 71 631 605 3009 1525
Fragments arranged by size:
3009 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2448 6065 2448
Size: 3617 2453
BlpI GC'TnA_GC
Cuts at: 3487 3487
Size: 6070
Bme1580I G_kGCm'C
Cuts at: 1790 4131 5287 1790
Size: 2341 1156 2573
Fragments arranged by size:
2573 2341 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3801 4759 442
Size: 416 53 83 399 24 2384 958 1753
Fragments arranged by size:
2384 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2511 2804 4730 1239
Size: 1272 293 1926 2579
Fragments arranged by size:
2579 1926 1272 293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2390 2897 4195 5060 5358 2390
Size: 507 1298 865 298 3102
Fragments arranged by size:
3102 1298 865 507 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2394 4733 882
Size: 617 895 2339 2219
Fragments arranged by size:
2339 2219 895 617
BsaAI yAC'GTr
Cuts at: 493 1204 3861 493
Size: 711 2657 2702
Fragments arranged by size:
2702 2657 711
BsaBI GATnn'nnATC
Cuts at: 2316 6064 2316
Size: 3748 2322
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3877 4259 4894 275
Size: 53 83 186 936 2344 382 635 1451
Fragments arranged by size:
2344 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4503 4901 5175 5322 5733 4503
Size: 398 274 147 411 4840
Fragments arranged by size:
4840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2777 2807 2840 2870 1178
Size: 30 165 30 1374 30 33 30 4378
Fragments arranged by size:
4378 1374 165 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2318 3038 3065 3353 3356 1186
Size: 949 183 720 27 288 3 3900
Fragments arranged by size:
3900 949 720 288 183 27 3
BseYI C'CCAG_C
Cuts at: 5273 5769 5273
Size: 496 5574
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3084 1163
Size: 573 1348 4149
Fragments arranged by size:
4149 1348 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3520 4281 5309 1540
Size: 275 186 1519 761 1028 2301
Fragments arranged by size:
2301 1519 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4131 4216 5287 4131
Size: 85 1071 4914
Fragments arranged by size:
4914 1071 85
BsmI GAATG_Cn'
Cuts at: 2361 3193 3581 3674 2361
Size: 832 388 93 4757
Fragments arranged by size:
4757 832 388 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2016 2016
Size: 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3389 4131 4216 5287 1391
Size: 399 1599 742 85 1071 2174
Fragments arranged by size:
2174 1599 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 3959 5689 3959
Size: 1730 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3425 844
Size: 2581 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3957 1394
Size: 429 2134 3507
Fragments arranged by size:
3507 2134 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4565 4739 65
Size: 1183 3317 174 1396
Fragments arranged by size:
3317 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4714 4901 1700
Size: 110 2904 187 2869
Fragments arranged by size:
2904 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6070
BssSI C'ACGA_G
Cuts at: 2803 4130 5142 2803
Size: 1327 1012 3731
Fragments arranged by size:
3731 1327 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6070
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6070
BstXI CCAn_nnnn'nTGG
Cuts at: 2210 2210
Size: 6070
BstYI r'GATC_y
Cuts at: 1972 2448 3400 3767 4152 4169 5621 5707 5719
Size: 476 952 367 385 17 1452 86 12
Cuts at: 5719 6065 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 952 476 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2516 3109 513
Size: 552 1451 593 3474
Fragments arranged by size:
3474 1451 593 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5331
Fragments arranged by size:
5331 683 56
BtsI GCAGTG_nn'
Cuts at: 3586 4380 4400 3586
Size: 794 20 5256
Fragments arranged by size:
5256 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 2623 2949 3374 2623
Size: 326 425 5319
Fragments arranged by size:
5319 425 326
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2977 5077 809
Size: 507 1661 2100 1802
Fragments arranged by size:
2100 1802 1661 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2753 3517 4406
Size: 54 1425 50 275 18 923 764 889
Cuts at: 4406 6035 8
Size: 1629 43
Fragments arranged by size:
1629 1425 923 889 764 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3517 1537
Size: 275 1705 4090
Fragments arranged by size:
4090 1705 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4000 1353
Size: 2647 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2861 4657 5031 5177 387
Size: 1064 138 1272 1796 374 146 1280
Fragments arranged by size:
1796 1280 1272 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2511 2804 4133 4730 5517 5785 1239
Size: 393 879 293 1329 597 787 268 1524
Fragments arranged by size:
1524 1329 879 787 597 393 293 268
EcoO109I rG'GnC_Cy
Cuts at: 2614 2614
Size: 6070
EcoRI G'AATT_C
Cuts at: 3229 3379 3757 3229
Size: 150 378 5542
Fragments arranged by size:
5542 378 150
EcoRV GAT'ATC
Cuts at: 2112 3160 2112
Size: 1048 5022
FspI TGC'GCA
Cuts at: 4576 4576
Size: 6070
HaeII r_GCGC'y
Cuts at: 1678 5217 1678
Size: 3539 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2080 2112 2393 2425 2777
Size: 32 135 32 508 32 281 32 352
Cuts at: 2777 2809 3061 3093 3149 3181 4711 4743 4785
Size: 32 252 32 56 32 1530 32 42
Cuts at: 4785 4817 1373
Size: 32 2626
Fragments arranged by size:
2626 1530 508 352 281 252 135 56
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3271 3414 3660 669
Size: 988 281 1333 143 246 3079
Fragments arranged by size:
3079 1333 988 281 246 143
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6070
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2485 3006 5184 5368 1305
Size: 1180 521 2178 184 2007
Fragments arranged by size:
2178 2007 1180 521 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4248
Fragments arranged by size:
4248 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4028 4387 4546 518
Size: 648 2862 359 159 2042
Fragments arranged by size:
2862 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3486 4163 5311 5556 1606
Size: 1880 677 1148 245 2120
Fragments arranged by size:
2120 1880 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2516 3109 513
Size: 552 1451 593 3474
Fragments arranged by size:
3474 1451 593 552
NdeI CA'TA_TG
Cuts at: 387 2370 387
Size: 1983 4087
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NsiI A_TGCA'T
Cuts at: 2361 3195 2361
Size: 834 5236
NspI r_CATG'y
Cuts at: 1132 1229 2497 3217 3428 4973 1132
Size: 97 1268 720 211 1545 2229
Fragments arranged by size:
2229 1545 1268 720 211 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6070
PciI A'CATG_T
Cuts at: 2493 4969 2493
Size: 2476 3594
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6070
PfoI T'CCnGG_A
Cuts at: 2781 2781
Size: 6070
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2614 2614
Size: 6070
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6070
PsiI TTA'TAA
Cuts at: 3640 3640
Size: 6070
PstI C_TGCA'G
Cuts at: 830 3132 3422 830
Size: 2302 290 3478
Fragments arranged by size:
3478 2302 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SbfI CC_TGCA'GG
Cuts at: 3422 3422
Size: 6070
ScaI AGT'ACT
Cuts at: 4318 6030 4318
Size: 1712 4358
SfcI C'TryA_G
Cuts at: 826 1045 3128 3418 4553 5234 5425 826
Size: 219 2083 290 1135 681 191 1471
Fragments arranged by size:
2083 1471 1135 681 290 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 3397 1872
Size: 1525 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2405 2876 4174 5075 5337 820
Size: 197 926 462 471 1298 901 262 1553
Fragments arranged by size:
1553 1298 926 901 471 462 262 197
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3428 1132
Size: 2296 3774
SspI AAT'ATT
Cuts at: 5 52 3994 5
Size: 47 3942 2081
Fragments arranged by size:
3942 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6070
StyI C'CwwG_G
Cuts at: 513 1065 2499 2516 3109 3509 513
Size: 552 1434 17 593 400 3074
Fragments arranged by size:
3074 1434 593 552 400 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4097 4114 4267 4452 4897 325
Size: 1255 2517 17 153 185 445 1498
Fragments arranged by size:
2517 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4316 6028 96
Size: 274 80 33 51 1443 2339 1712 138
Fragments arranged by size:
2339 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2794 2826 2840 2872 6030 6062 2794
Size: 32 14 32 3158 32 2802
Fragments arranged by size:
3158 2802 32 32 32 14
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3142 3142
Size: 6070
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 3395 1870
Size: 1525 4545
XmnI GAAnn'nnTTC
Cuts at: 4199 4199
Size: 6070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3878 276
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI