Restriction map for
pFN21AE5568
(Circular) (Six-base) MAPSORT of: pFN21AE5568.seq Check: 8258 from: 1 to: 5998
pFN21AE5568 5998 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2485 3122 2485
Size: 637 5361
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 2108 3319 2108
Size: 1211 4787
AclI AA'CG_TT
Cuts at: 1563 2389 4125 4498 1563
Size: 826 1736 373 3063
Fragments arranged by size:
3063 1736 826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2716 3186 4061 5445 5713 1632
Size: 1084 470 875 1384 268 1917
Fragments arranged by size:
1917 1384 1084 875 470 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 2516 4897 5874 1203
Size: 1313 2381 977 1327
Fragments arranged by size:
2381 1327 1313 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 4727 4727
Size: 5998
AleI CACnn'nnGTG
Cuts at: 1166 3037 1166
Size: 1871 4127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2696 1184
Size: 1512 4486
AlwNI CAG_nnn'CTG
Cuts at: 3020 3100 3227 3741 5313 3020
Size: 80 127 514 1572 3705
Fragments arranged by size:
3705 1572 514 127 80
ApaI G_GGCC'C
Cuts at: 2773 2773
Size: 5998
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 3307 3533 3685 5794 1493
Size: 1814 226 152 2109 1697
Fragments arranged by size:
2109 1814 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 2467 2823 3323 1870
Size: 73 524 356 500 4545
Fragments arranged by size:
4545 524 500 356 73
AvrII C'CTAG_G
Cuts at: 2068 2068
Size: 5998
BamHI G'GATC_C
Cuts at: 3328 3695 3328
Size: 367 5631
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 2509 3134 3319 4774
Size: 325 217 627 321 401 625 185 1455
Cuts at: 4774 618
Size: 1842
Fragments arranged by size:
1842 1455 627 625 401 325 321 217
185
BanII G_rGCy'C
Cuts at: 1391 2190 2349 2556 2773 2909 2972 3032 3317
Size: 799 159 207 217 136 63 60 285
Cuts at: 3317 1391
Size: 4072
Fragments arranged by size:
4072 799 285 217 207 159 136 63
60
BbeI G_GCGC'C
Cuts at: 2513 2513
Size: 5998
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3060 3094 4189 4223 1903
Size: 34 1123 34 1095 34 3678
Fragments arranged by size:
3678 1123 1095 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5998
BfrBI ATG'CAT
Cuts at: 2560 2560
Size: 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2856 4609 136
Size: 107 122 71 631 605 1184 1753 1525
Fragments arranged by size:
1753 1525 1184 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5993 5993
Size: 5998
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 2773 3139 4059 5215 1790
Size: 983 366 920 1156 2573
Fragments arranged by size:
2573 1156 983 920 366
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2769 2973 3729
Size: 416 53 83 399 24 1352 204 756
Cuts at: 3729 4687 442
Size: 958 1753
Fragments arranged by size:
1753 1352 958 756 416 399 204 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2928 2928
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 2314 2387 2507 3147 4658 1239
Size: 832 243 73 120 640 1511 2579
Fragments arranged by size:
2579 1511 832 640 243 120 73
Bpu10I CC'TnA_GC
Cuts at: 3150 3150
Size: 5998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4123 4988 5286 4123
Size: 865 298 4835
Fragments arranged by size:
4835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2027 2506 4661 882
Size: 617 528 479 2155 2219
Fragments arranged by size:
2219 2155 617 528 479
BsaAI yAC'GTr
Cuts at: 493 1204 2397 2517 3789 493
Size: 711 1193 120 1272 2702
Fragments arranged by size:
2702 1272 1193 711 120
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2510 2687 3805 4187
Size: 53 83 186 936 977 177 1118 382
Cuts at: 4187 4822 275
Size: 635 1451
Fragments arranged by size:
1451 1118 977 936 635 382 186 177
83 53
BsaWI w'CCGG_w
Cuts at: 4431 4829 5103 5250 5661 4431
Size: 398 274 147 411 4768
Fragments arranged by size:
4768 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2936 2966 1178
Size: 30 165 30 1533 30 4210
Fragments arranged by size:
4210 1533 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2692 1186
Size: 1506 4492
BseYI C'CCAG_C
Cuts at: 2038 2348 2566 3241 5201 5697 2038
Size: 310 218 675 1960 496 2339
Fragments arranged by size:
2339 1960 675 496 310 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3090 1163
Size: 573 1354 4071
Fragments arranged by size:
4071 1354 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2246 2599 3448 4209 5237 1540
Size: 275 186 245 353 849 761 1028 2301
Fragments arranged by size:
2301 1028 849 761 353 275 245 186
BsiHKAI G_wGCw'C
Cuts at: 2556 2586 4059 4144 5215 2556
Size: 30 1473 85 1071 3339
Fragments arranged by size:
3339 1473 1071 85 30
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2349 2556 2586 2773 2909 2972
Size: 399 400 159 207 30 187 136 63
Cuts at: 2972 3032 3139 3317 4059 4144 5215 1391
Size: 60 107 178 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 207 187 178
159 136 107 85 63 60 30
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 2548 3887 5617 2548
Size: 1339 1730 2929
Fragments arranged by size:
2929 1730 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2485 3122 3353 844
Size: 1641 637 231 3489
Fragments arranged by size:
3489 1641 637 231
BsrBI CCG'CTC
Cuts at: 1394 1823 2912 3885 1394
Size: 429 1089 973 3507
Fragments arranged by size:
3507 1089 973 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4493 4667 65
Size: 1183 3245 174 1396
Fragments arranged by size:
3245 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2736 4642 4829 1700
Size: 110 926 1906 187 2869
Fragments arranged by size:
2869 1906 926 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 2579 4058 5070 2579
Size: 1479 1012 3507
Fragments arranged by size:
3507 1479 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5998
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstXI CCAn_nnnn'nTGG
Cuts at: 2897 3039 2897
Size: 142 5856
BstYI r'GATC_y
Cuts at: 1972 2631 3328 3695 4080 4097 5549 5635 5647
Size: 659 697 367 385 17 1452 86 12
Cuts at: 5647 5993 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 697 659 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2136 2136
Size: 5998
BtgI C'CryG_G
Cuts at: 513 1065 2004 2289 2869 513
Size: 552 939 285 580 3642
Fragments arranged by size:
3642 939 580 552 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2809 487
Size: 683 56 1583 3676
Fragments arranged by size:
3676 1583 683 56
BtsI GCAGTG_nn'
Cuts at: 2488 3514 4308 4328 2488
Size: 1026 794 20 4158
Fragments arranged by size:
4158 1026 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2243 2788 3168
Size: 54 1425 50 275 18 413 545 380
Cuts at: 3168 3445 4334 5963 8
Size: 277 889 1629 43
Fragments arranged by size:
1629 1425 889 545 413 380 277 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2243 3445 1537
Size: 275 431 1202 4090
Fragments arranged by size:
4090 1202 431 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3928 1353
Size: 2575 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2972 4585 4959 5105 387
Size: 1064 138 1383 1613 374 146 1280
Fragments arranged by size:
1613 1383 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2554 2554
Size: 5998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2314 2387 2507 2716 3147 3186
Size: 393 439 243 73 120 209 431 39
Cuts at: 3186 4061 4658 5445 5713 1239
Size: 875 597 787 268 1524
Fragments arranged by size:
1524 875 787 597 439 431 393 268
243 209 120 73 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 3082 3129 2225
Size: 857 47 5094
Fragments arranged by size:
5094 857 47
EcoO109I rG'GnC_Cy
Cuts at: 2314 2770 2827 2865 2880 3017 2314
Size: 456 57 38 15 137 5295
Fragments arranged by size:
5295 456 137 57 38 15
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
FspI TGC'GCA
Cuts at: 2095 4504 2095
Size: 2409 3589
HaeII r_GCGC'y
Cuts at: 1678 2078 2513 5145 1678
Size: 400 435 2632 2531
Fragments arranged by size:
2632 2531 435 400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2687 2719 2936 2968 4639
Size: 32 135 32 1115 32 217 32 1671
Cuts at: 4639 4671 4713 4745 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1671 1115 217 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2620 3342 3588 669
Size: 988 281 682 722 246 3079
Fragments arranged by size:
3079 988 722 682 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KasI G'GCGC_C
Cuts at: 2509 2509
Size: 5998
KpnI G_GTAC'C
Cuts at: 2112 3323 2112
Size: 1211 4787
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2281 2857 5112 5296 1305
Size: 976 576 2255 184 2007
Fragments arranged by size:
2255 2007 976 576 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4176
Fragments arranged by size:
4176 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2450 2561 3037 3956 4315 4474 518
Size: 648 1284 111 476 919 359 159 2042
Fragments arranged by size:
2042 1284 919 648 476 359 159 111
MspA1I CmG'CkG
Cuts at: 1606 2091 2935 3414 4091 5239 5484 1606
Size: 485 844 479 677 1148 245 2120
Fragments arranged by size:
2120 1148 844 677 485 479 245
NaeI GCC'GGC
Cuts at: 2738 2738
Size: 5998
NarI GG'CG_CC
Cuts at: 2510 2510
Size: 5998
NcoI C'CATG_G
Cuts at: 513 1065 2004 2289 513
Size: 552 939 285 4222
Fragments arranged by size:
4222 939 552 285
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NgoMIV G'CCGG_C
Cuts at: 2736 2736
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NsiI A_TGCA'T
Cuts at: 2562 2562
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 2564 2683 3356 4901 1132
Size: 97 1335 119 673 1545 2229
Fragments arranged by size:
2229 1545 1335 673 119 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5998
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5998
PfoI T'CCnGG_A
Cuts at: 2193 2364 2963 2193
Size: 171 599 5228
Fragments arranged by size:
5228 599 171
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PmlI CAC'GTG
Cuts at: 2397 2517 2397
Size: 120 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2314 2827 2880 2314
Size: 513 53 5432
Fragments arranged by size:
5432 513 53
PshAI GACnn'nnGTC
Cuts at: 1613 2263 1613
Size: 650 5348
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PspOMI G'GGCC_C
Cuts at: 2769 2769
Size: 5998
PstI C_TGCA'G
Cuts at: 830 2849 2934 3016 3350 830
Size: 2019 85 82 334 3478
Fragments arranged by size:
3478 2019 334 85 82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 2091 2091
Size: 5998
SacI G_AGCT'C
Cuts at: 2556 2556
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SanDI GG'GwC_CC
Cuts at: 2880 2880
Size: 5998
SbfI CC_TGCA'GG
Cuts at: 3016 3350 3016
Size: 334 5664
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SfcI C'TryA_G
Cuts at: 826 1045 2845 2930 3012 3346 4481 5162 5353
Size: 219 1800 85 82 334 1135 681 191
Cuts at: 5353 826
Size: 1471
Fragments arranged by size:
1800 1471 1135 681 334 219 191 85
82
SfoI GGC'GCC
Cuts at: 2511 2511
Size: 5998
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 2825 3325 1872
Size: 953 500 4545
Fragments arranged by size:
4545 953 500
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2467 4102 5003 5265 820
Size: 197 926 524 1635 901 262 1553
Fragments arranged by size:
1635 1553 926 901 524 262 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 2150 3077 3124 1517
Size: 633 927 47 4391
Fragments arranged by size:
4391 927 633 47
StyI C'CwwG_G
Cuts at: 513 1065 2004 2030 2068 2182 2289 3437 513
Size: 552 939 26 38 114 107 1148 3074
Fragments arranged by size:
3074 1148 939 552 114 107 38 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 2282 2448 3027 4025 4042 4195
Size: 1255 443 259 166 579 998 17 153
Cuts at: 4195 4380 4825 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 998 579 445 443 259 185
166 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4244 5956 96
Size: 274 80 33 51 1443 2267 1712 138
Fragments arranged by size:
2267 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2791 2823 5958 5990 2791
Size: 32 3135 32 2799
Fragments arranged by size:
3135 2799 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 2467 1943
Size: 524 5474
XmaI C'CCGG_G
Cuts at: 1870 2823 3323 1870
Size: 953 500 4545
Fragments arranged by size:
4545 953 500
XmnI GAAnn'nnTTC
Cuts at: 4127 4127
Size: 5998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BsiWI BsmBI BstZ17I
DraIII EcoRV FalI FseI FspAI NruI PacI PspXI
PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI
XcmI