Restriction map for
pFN21AE5566
(Circular) (Six-base) MAPSORT of: pFN21AE5566.seq Check: 284 from: 1 to: 6022
pFN21AE5566 6022 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3832 278
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2881 3365 1937
Size: 944 484 4594
Fragments arranged by size:
4594 944 484
Acc65I G'GTAC_C
Cuts at: 2229 3343 2229
Size: 1114 4908
AclI AA'CG_TT
Cuts at: 1563 4149 4522 1563
Size: 2586 373 3063
Fragments arranged by size:
3063 2586 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2487 2726 4085 5469 5737 1632
Size: 855 239 1359 1384 268 1917
Fragments arranged by size:
1917 1384 1359 855 268 239
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5825
AflIII A'CryG_T
Cuts at: 1203 4921 5898 1203
Size: 3718 977 1327
Fragments arranged by size:
3718 1327 977
AgeI A'CCGG_T
Cuts at: 4853 4853
Size: 6022
AhdI GACnn_n'nnGTC
Cuts at: 4751 4751
Size: 6022
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6022
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6022
AlwNI CAG_nnn'CTG
Cuts at: 2346 3765 5337 2346
Size: 1419 1572 3031
Fragments arranged by size:
3031 1572 1419
ApaLI G'TGCA_C
Cuts at: 4079 5235 4079
Size: 1156 4866
ApoI r'AATT_y
Cuts at: 1493 2938 3331 3557 3709 5818 1493
Size: 1445 393 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 393 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4576 160
Size: 872 3544 1606
Fragments arranged by size:
3544 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
AvaI C'yCGr_G
Cuts at: 1870 1943 3347 1870
Size: 73 1404 4545
Fragments arranged by size:
4545 1404 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2221 2254 2221
Size: 33 5989
BamHI G'GATC_C
Cuts at: 2536 3352 3719 2536
Size: 816 367 4839
Fragments arranged by size:
4839 816 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2229 3343 4798 618
Size: 325 217 627 442 1114 1455 1842
Fragments arranged by size:
1842 1455 1114 627 442 325 217
BanII G_rGCy'C
Cuts at: 1391 3341 1391
Size: 1950 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2193 928
Size: 971 294 4757
Fragments arranged by size:
4757 971 294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2057 2091 3237 3271 4213 4247 1903
Size: 34 120 34 1146 34 942 34 3678
Fragments arranged by size:
3678 1146 942 120 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3914 5124 3914
Size: 1210 4812
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6022
BfrBI ATG'CAT
Cuts at: 2914 2914
Size: 6022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4633 136
Size: 107 122 71 631 605 2961 1525
Fragments arranged by size:
2961 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6017 6017
Size: 6022
BlpI GC'TnA_GC
Cuts at: 3439 3439
Size: 6022
Bme1580I G_kGCm'C
Cuts at: 1790 4083 5239 1790
Size: 2293 1156 2573
Fragments arranged by size:
2573 2293 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6022
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3753 4711 442
Size: 416 53 83 399 24 2336 958 1753
Fragments arranged by size:
2336 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4682 1239
Size: 3443 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2291 4147 5012 5310 2291
Size: 1856 865 298 3003
Fragments arranged by size:
3003 1856 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4685 882
Size: 617 3186 2219
Fragments arranged by size:
3186 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3813 493
Size: 711 2609 2702
Fragments arranged by size:
2702 2609 711
BsaBI GATnn'nnATC
Cuts at: 6016 6016
Size: 6022
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3829 4211 4846
Size: 53 83 186 936 476 1820 382 635
Cuts at: 4846 275
Size: 1451
Fragments arranged by size:
1820 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2043 4455 4853 5127 5274 5685 2043
Size: 2412 398 274 147 411 2380
Fragments arranged by size:
2412 2380 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5797
Fragments arranged by size:
5797 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 1186
Size: 898 5124
BseYI C'CCAG_C
Cuts at: 2965 3299 5225 5721 2965
Size: 334 1926 496 3266
Fragments arranged by size:
3266 1926 496 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 2370 1163
Size: 573 315 319 4815
Fragments arranged by size:
4815 573 319 315
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2044 2067 3472 4233 5261 1540
Size: 275 186 43 23 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186 43 23
BsiHKAI G_wGCw'C
Cuts at: 4083 4168 5239 4083
Size: 85 1071 4866
Fragments arranged by size:
4866 1071 85
BsmI GAATG_Cn'
Cuts at: 2914 3533 3626 2914
Size: 619 93 5310
Fragments arranged by size:
5310 619 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3341 4083 4168 5239 1391
Size: 399 1551 742 85 1071 2174
Fragments arranged by size:
2174 1551 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5685 5685
Size: 6022
BspHI T'CATG_A
Cuts at: 3126 3911 5641 3126
Size: 785 1730 3507
Fragments arranged by size:
3507 1730 785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3377 844
Size: 2533 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3909 1394
Size: 429 2086 3507
Fragments arranged by size:
3507 2086 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4517 4691 65
Size: 1183 3269 174 1396
Fragments arranged by size:
3269 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4666 4853 1700
Size: 110 2856 187 2869
Fragments arranged by size:
2869 2856 187 110
BsrGI T'GTAC_A
Cuts at: 96 2759 96
Size: 2663 3359
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6022
BssSI C'ACGA_G
Cuts at: 4082 5094 4082
Size: 1012 5010
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2382 794
Size: 1588 4434
BstBI TT'CG_AA
Cuts at: 4826 4826
Size: 6022
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6022
BstXI CCAn_nnnn'nTGG
Cuts at: 2226 2226
Size: 6022
BstYI r'GATC_y
Cuts at: 1972 2245 2536 3352 3719 4104 4121 5573 5659
Size: 273 291 816 367 385 17 1452 86
Cuts at: 5659 5671 6017 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 816 385 367 346 291 273
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4531
Fragments arranged by size:
4531 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5283
Fragments arranged by size:
5283 683 56
BtsI GCAGTG_nn'
Cuts at: 3313 3538 4332 4352 3313
Size: 225 794 20 4983
Fragments arranged by size:
4983 794 225 20
ClaI AT'CG_AT
Cuts at: 1554 2413 1554
Size: 859 5163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5987
DraI TTT'AAA
Cuts at: 2314 3326 2314
Size: 1012 5010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5029 809
Size: 507 3713 1802
Fragments arranged by size:
3713 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 3469 4358
Size: 54 1425 50 275 18 234 1405 889
Cuts at: 4358 5987 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1405 889 275 234 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2064 3469 1537
Size: 275 252 1405 4090
Fragments arranged by size:
4090 1405 275 252
EarI CTCTTCn'nnn_
Cuts at: 1353 2526 2666 2745 3055 3952 1353
Size: 1173 140 79 310 897 3423
Fragments arranged by size:
3423 1173 897 310 140 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4609 4983 5129 387
Size: 1064 138 3020 374 146 1280
Fragments arranged by size:
3020 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2487 2726 4085 4682 5469 5737 1239
Size: 393 855 239 1359 597 787 268 1524
Fragments arranged by size:
1524 1359 855 787 597 393 268 239
EcoO109I rG'GnC_Cy
Cuts at: 2162 2483 2162
Size: 321 5701
EcoRI G'AATT_C
Cuts at: 3331 3709 3331
Size: 378 5644
FspI TGC'GCA
Cuts at: 4528 4528
Size: 6022
HaeII r_GCGC'y
Cuts at: 1678 5169 1678
Size: 3491 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2995 3027 3097 3129 4663
Size: 32 135 32 1423 32 70 32 1534
Cuts at: 4663 4695 4737 4769 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1534 1423 135 70 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2755 3366 3612 669
Size: 988 281 817 611 246 3079
Fragments arranged by size:
3079 988 817 611 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6022
HpaI GTT'AAC
Cuts at: 2755 3612 2755
Size: 857 5165
KpnI G_GTAC'C
Cuts at: 2233 3347 2233
Size: 1114 4908
MfeI C'AATT_G
Cuts at: 3621 3621
Size: 6022
MluI A'CGCG_T
Cuts at: 5898 5898
Size: 6022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1992 2384 5136 5320 1305
Size: 687 392 2752 184 2007
Fragments arranged by size:
2752 2007 687 392 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4200
Fragments arranged by size:
4200 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 3980 4339 4498 518
Size: 648 1132 1682 359 159 2042
Fragments arranged by size:
2042 1682 1132 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3438 4115 5263 5508 1606
Size: 1832 677 1148 245 2120
Fragments arranged by size:
2120 1832 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4531
Fragments arranged by size:
4531 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6022
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6022
NotI GC'GGCC_GC
Cuts at: 3469 3469
Size: 6022
NsiI A_TGCA'T
Cuts at: 2916 2916
Size: 6022
NspI r_CATG'y
Cuts at: 1132 1229 2194 2207 2695 3380 4925 1132
Size: 97 965 13 488 685 1545 2229
Fragments arranged by size:
2229 1545 965 685 488 97 13
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6022
PciI A'CATG_T
Cuts at: 4921 4921
Size: 6022
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3090 1108
Size: 1982 4040
PmeI GTTT'AAAC
Cuts at: 3326 3326
Size: 6022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4091 4123 5604 5636 4091
Size: 32 1481 32 4477
Fragments arranged by size:
4477 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2162 2483 2162
Size: 321 5701
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6022
PsiI TTA'TAA
Cuts at: 2580 3592 2580
Size: 1012 5010
PstI C_TGCA'G
Cuts at: 830 2159 3374 830
Size: 1329 1215 3478
Fragments arranged by size:
3478 1329 1215
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6022
SalI G'TCGA_C
Cuts at: 1936 3364 1936
Size: 1428 4594
SanDI GG'GwC_CC
Cuts at: 2162 2162
Size: 6022
SbfI CC_TGCA'GG
Cuts at: 3374 3374
Size: 6022
ScaI AGT'ACT
Cuts at: 4270 5982 4270
Size: 1712 4310
SfcI C'TryA_G
Cuts at: 826 1045 2155 2790 3370 4505 5186 5377 826
Size: 219 1110 635 580 1135 681 191 1471
Fragments arranged by size:
1471 1135 1110 681 635 580 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6022
SmaI CCC'GGG
Cuts at: 1872 3349 1872
Size: 1477 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2270 4126 5027 5289 820
Size: 197 926 327 1856 901 262 1553
Fragments arranged by size:
1856 1553 926 901 327 262 197
SnaBI TAC'GTA
Cuts at: 493 3813 493
Size: 3320 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6022
SphI G_CATG'C
Cuts at: 1132 2695 3380 1132
Size: 1563 685 3774
Fragments arranged by size:
3774 1563 685
SspI AAT'ATT
Cuts at: 5 52 2619 3946 5
Size: 47 2567 1327 2081
Fragments arranged by size:
2567 2081 1327 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6022
StyI C'CwwG_G
Cuts at: 513 1065 2004 3461 513
Size: 552 939 1457 3074
Fragments arranged by size:
3074 1457 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4049 4066 4219 4404 4849 325
Size: 1255 2469 17 153 185 445 1498
Fragments arranged by size:
2469 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2759 4268 5980
Size: 274 80 33 51 1443 782 1509 1712
Cuts at: 5980 96
Size: 138
Fragments arranged by size:
1712 1509 1443 782 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5982 6014 5982
Size: 32 5990
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6022
XbaI T'CTAG_A
Cuts at: 3358 3358
Size: 6022
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6022
XmaI C'CCGG_G
Cuts at: 1870 3347 1870
Size: 1477 4545
XmnI GAAnn'nnTTC
Cuts at: 4151 4151
Size: 6022
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3830 276
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI