Restriction map for
pFN21AE5553
(Circular) (Six-base) MAPSORT of: pFN21AE5553.seq Check: 6873 from: 1 to: 5983
pFN21AE5553 5983 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2880 3326 1937
Size: 943 446 4594
Fragments arranged by size:
4594 943 446
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 2692 2991 4046 5430 5698 1632
Size: 443 617 299 1055 1384 268 1917
Fragments arranged by size:
1917 1384 1055 617 443 299 268
AflII C'TTAA_G
Cuts at: 820 1017 3047 820
Size: 197 2030 3756
Fragments arranged by size:
3756 2030 197
AflIII A'CryG_T
Cuts at: 1203 2034 4882 5859 1203
Size: 831 2848 977 1327
Fragments arranged by size:
2848 1327 977 831
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5983
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2021 2053 2060 2092 2021
Size: 32 7 32 5912
Fragments arranged by size:
5912 32 32 7
AlwNI CAG_nnn'CTG
Cuts at: 3726 5298 3726
Size: 1572 4411
ApaLI G'TGCA_C
Cuts at: 4040 5196 4040
Size: 1156 4827
ApoI r'AATT_y
Cuts at: 1493 2617 3292 3518 3670 5779 1493
Size: 1124 675 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 675 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 3308 1870
Size: 73 1365 4545
Fragments arranged by size:
4545 1365 73
BamHI G'GATC_C
Cuts at: 2757 3313 3680 2757
Size: 556 367 5060
Fragments arranged by size:
5060 556 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3304 4759 618
Size: 325 217 627 1517 1455 1842
Fragments arranged by size:
1842 1517 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 2073 3201 3302 1391
Size: 673 9 1128 101 4072
Fragments arranged by size:
4072 1128 673 101 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2612 2915 928
Size: 971 713 303 3996
Fragments arranged by size:
3996 971 713 303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4174 4208 1903
Size: 34 2237 34 3678
Fragments arranged by size:
3678 2237 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 3120 1548
Size: 1572 4411
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4594 136
Size: 107 122 71 631 605 2922 1525
Fragments arranged by size:
2922 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2175 5978 2175
Size: 3803 2180
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2784 4044 5200 1790
Size: 994 1260 1156 2573
Fragments arranged by size:
2573 1260 1156 994
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3714 4672 442
Size: 416 53 83 399 24 2297 958 1753
Fragments arranged by size:
2297 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 3077 4643 1239
Size: 875 963 1566 2579
Fragments arranged by size:
2579 1566 963 875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4108 4973 5271 4108
Size: 865 298 4820
Fragments arranged by size:
4820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4646 882
Size: 617 3147 2219
Fragments arranged by size:
3147 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2035 2121 3774 493
Size: 711 831 86 1653 2702
Fragments arranged by size:
2702 1653 831 711 86
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2227 3790 4172 4807
Size: 53 83 186 936 694 1563 382 635
Cuts at: 4807 275
Size: 1451
Fragments arranged by size:
1563 1451 936 694 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4416 4814 5088 5235 5646 4416
Size: 398 274 147 411 4753
Fragments arranged by size:
4753 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2021 2051 2060 2090 1178
Size: 30 165 30 618 30 9 30 5071
Fragments arranged by size:
5071 618 165 30 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2391 2707 3188 1186
Size: 1205 316 481 3981
Fragments arranged by size:
3981 1205 481 316
BseYI C'CCAG_C
Cuts at: 2256 5186 5682 2256
Size: 2930 496 2557
Fragments arranged by size:
2930 2557 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2761 1163
Size: 573 1025 4385
Fragments arranged by size:
4385 1025 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 4194 5222 1540
Size: 275 186 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3201 4044 4129 5200 3201
Size: 843 85 1071 3984
Fragments arranged by size:
3984 1071 843 85
BsmI GAATG_Cn'
Cuts at: 3256 3494 3587 3256
Size: 238 93 5652
Fragments arranged by size:
5652 238 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2073 2784 3201 3302 4044 4129
Size: 399 274 9 711 417 101 742 85
Cuts at: 4129 5200 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 711 417 399 274 101
85 9
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 3872 5602 3872
Size: 1730 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2376 3338 844
Size: 1532 962 3489
Fragments arranged by size:
3489 1532 962
BsrBI CCG'CTC
Cuts at: 1394 1823 3870 1394
Size: 429 2047 3507
Fragments arranged by size:
3507 2047 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2124 2805 4478 4652 65
Size: 1183 876 681 1673 174 1396
Fragments arranged by size:
1673 1396 1183 876 681 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4627 4814 1700
Size: 110 2817 187 2869
Fragments arranged by size:
2869 2817 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 4043 5055 4043
Size: 1012 4971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2480 794
Size: 1686 4297
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 2175 2757 3313 3680 4065 4082 5534 5620
Size: 203 582 556 367 385 17 1452 86
Cuts at: 5620 5632 5978 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 582 556 385 367 346 203
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 2064 2811 2871 513
Size: 552 939 21 39 747 60 3625
Fragments arranged by size:
3625 939 747 552 60 39 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 1996 487
Size: 683 56 770 4474
Fragments arranged by size:
4474 770 683 56
BtsI GCAGTG_nn'
Cuts at: 2282 3079 3499 4293 4313 2282
Size: 797 420 794 20 3952
Fragments arranged by size:
3952 797 794 420 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2534 2569 529
Size: 35 1970 35 3943
Fragments arranged by size:
3943 1970 35 35
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2114 4990 809
Size: 507 798 2876 1802
Fragments arranged by size:
2876 1802 798 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2022 3430
Size: 54 1425 50 275 18 177 15 1408
Cuts at: 3430 4319 5948 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1408 889 275 177 54 50
43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3008 3913 1353
Size: 1655 905 3423
Fragments arranged by size:
3423 1655 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4570 4944 5090 387
Size: 1064 138 2981 374 146 1280
Fragments arranged by size:
2981 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3199 3199
Size: 5983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2114 2692 2991 3077 4046 4643
Size: 393 443 39 578 299 86 969 597
Cuts at: 4643 5430 5698 1239
Size: 787 268 1524
Fragments arranged by size:
1524 969 787 597 578 443 393 299
268 86 39
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 5130 1678
Size: 3452 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3017 3049 3061 3093 4624
Size: 32 135 32 1445 32 12 32 1531
Cuts at: 4624 4656 4698 4730 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1531 1445 135 42 32 32 32
32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 2586 3582 2586
Size: 996 4987
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2926 5097 5281 1305
Size: 1621 2171 184 2007
Fragments arranged by size:
2171 2007 1621 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2024 10
Size: 54 1425 343 177 15 3969
Fragments arranged by size:
3969 1425 343 177 54 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 2810 3040 3941 4300 4459 518
Size: 648 963 681 230 901 359 159 2042
Fragments arranged by size:
2042 963 901 681 648 359 230 159
MspA1I CmG'CkG
Cuts at: 1606 2377 2396 3399 4076 5224 5469 1606
Size: 771 19 1003 677 1148 245 2120
Fragments arranged by size:
2120 1148 1003 771 677 245 19
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4492
Fragments arranged by size:
4492 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 3341 4886 1132
Size: 97 2112 1545 2229
Fragments arranged by size:
2229 2112 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5983
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5983
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PmlI CAC'GTG
Cuts at: 2121 2121
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2021 2053 2060 2092 4052 4084 5565 5597 2021
Size: 32 7 32 1960 32 1481 32 2407
Fragments arranged by size:
2407 1960 1481 32 32 32 32 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PstI C_TGCA'G
Cuts at: 830 2289 3163 3335 830
Size: 1459 874 172 3478
Fragments arranged by size:
3478 1459 874 172
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
PvuII CAG'CTG
Cuts at: 2396 2396
Size: 5983
SacI G_AGCT'C
Cuts at: 3201 3201
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 2600 4231 5943 2600
Size: 1631 1712 2640
Fragments arranged by size:
2640 1712 1631
SfcI C'TryA_G
Cuts at: 826 1045 2285 3159 3331 4466 5147 5338 826
Size: 219 1240 874 172 1135 681 191 1471
Fragments arranged by size:
1471 1240 1135 874 681 219 191 172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 3310 1872
Size: 1438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3047 4087 4988 5250 820
Size: 197 926 1104 1040 901 262 1553
Fragments arranged by size:
1553 1104 1040 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 3341 1132
Size: 2209 3774
SspI AAT'ATT
Cuts at: 5 52 3064 3907 5
Size: 47 3012 843 2081
Fragments arranged by size:
3012 2081 843 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5983
StyI C'CwwG_G
Cuts at: 513 1065 2004 3112 3202 3422 513
Size: 552 939 1108 90 220 3074
Fragments arranged by size:
3074 1108 939 552 220 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4010 4027 4180 4365 4810 325
Size: 1255 2430 17 153 185 445 1498
Fragments arranged by size:
2430 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2598 2640 4229
Size: 274 80 33 51 1443 621 42 1589
Cuts at: 4229 5941 96
Size: 1712 138
Fragments arranged by size:
1712 1589 1443 621 274 138 80 51
42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5943 5975 5943
Size: 32 5951
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2017 2017
Size: 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 3308 1870
Size: 1438 4545
XmnI GAAnn'nnTTC
Cuts at: 4112 4112
Size: 5983
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI