Restriction map for
pFN21AE5552
(Circular) (Six-base) MAPSORT of: pFN21AE5552.seq Check: 7164 from: 1 to: 5956
pFN21AE5552 5956 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2013 3766 278
Size: 53 83 186 936 477 1753 2468
Fragments arranged by size:
2468 1753 936 477 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2175 2403 2796 3108 4019 5403 5671 1632
Size: 543 228 393 312 911 1384 268 1917
Fragments arranged by size:
1917 1384 911 543 393 312 268 228
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2179 2211 2179
Size: 32 5924
AlwNI CAG_nnn'CTG
Cuts at: 2198 3699 5271 2198
Size: 1501 1572 2883
Fragments arranged by size:
2883 1572 1501
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 2363 2476 2611 2805 3265 3491 3643 5752
Size: 870 113 135 194 460 226 152 2109
Cuts at: 5752 1493
Size: 1697
Fragments arranged by size:
2109 1697 870 460 226 194 152 135
113
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
BamHI G'GATC_C
Cuts at: 3158 3286 3653 3158
Size: 128 367 5461
Fragments arranged by size:
5461 367 128
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3277 4732 618
Size: 325 217 627 1490 1455 1842
Fragments arranged by size:
1842 1490 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2684 3275 1391
Size: 1293 591 4072
Fragments arranged by size:
4072 1293 591
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4985
BbvCI CC'TCA_GC
Cuts at: 2724 2724
Size: 5956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2303 3848 5058 2303
Size: 1545 1210 3201
Fragments arranged by size:
3201 1545 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4567 136
Size: 107 122 71 631 605 2895 1525
Fragments arranged by size:
2895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 4017 5173 1790
Size: 2227 1156 2573
Fragments arranged by size:
2573 2227 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3687 4645 442
Size: 416 53 83 399 24 2270 958 1753
Fragments arranged by size:
2270 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2006 2130 2183 3198 4616 1239
Size: 767 124 53 1015 1418 2579
Fragments arranged by size:
2579 1418 1015 767 124 53
Bpu10I CC'TnA_GC
Cuts at: 2199 2724 2199
Size: 525 5431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3045 4081 4946 5244 3045
Size: 1036 865 298 3757
Fragments arranged by size:
3757 1036 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 3763 4145 4780
Size: 53 83 186 936 477 1753 382 635
Cuts at: 4780 275
Size: 1451
Fragments arranged by size:
1753 1451 936 635 477 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2179 2209 1178
Size: 30 165 30 776 30 4925
Fragments arranged by size:
4925 776 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2388 2642 1186
Size: 1202 254 4500
Fragments arranged by size:
4500 1202 254
BseYI C'CCAG_C
Cuts at: 5159 5655 5159
Size: 496 5460
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5383
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2594 4017 4102 5173 2594
Size: 1423 85 1071 3377
Fragments arranged by size:
3377 1423 1071 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2594 2684 3275 4017 4102 5173 1391
Size: 399 804 90 591 742 85 1071 2174
Fragments arranged by size:
2174 1071 804 742 591 399 90 85
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3311 844
Size: 2467 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 2891 96
Size: 2795 3161
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 2332 3158 3286 3653 4038 4055 5507 5593
Size: 360 826 128 367 385 17 1452 86
Cuts at: 5593 5605 5951 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 826 385 367 360 346 128
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3201 513
Size: 552 939 1197 3268
Fragments arranged by size:
3268 1197 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 3472 4266 4286 3472
Size: 794 20 5142
Fragments arranged by size:
5142 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 2140 2445 3260 2140
Size: 305 815 4836
Fragments arranged by size:
4836 815 305
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3403 4292 5921
Size: 54 1425 50 275 18 1573 889 1629
Cuts at: 5921 8
Size: 43
Fragments arranged by size:
1629 1573 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2653 2892 2904 2913 2925 3009 3886
Size: 689 611 239 12 9 12 84 877
Cuts at: 3886 1353
Size: 3423
Fragments arranged by size:
3423 877 689 611 239 84 12 12
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2006 2130 2175 2183 2403 2796 3108
Size: 393 374 124 45 8 220 393 312
Cuts at: 3108 3198 4019 4616 5403 5671 1239
Size: 90 821 597 787 268 1524
Fragments arranged by size:
1524 821 787 597 393 393 374 312
268 220 124 90 45 8
EcoO109I rG'GnC_Cy
Cuts at: 2210 3107 2210
Size: 897 5059
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 2033 5103 1678
Size: 355 3070 2531
Fragments arranged by size:
3070 2531 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2943 2975 4597 4629 4671
Size: 32 135 32 1371 32 1622 32 42
Cuts at: 4671 4703 1373
Size: 32 2626
Fragments arranged by size:
2626 1622 1371 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2328 3300 3546 669
Size: 988 281 390 972 246 3079
Fragments arranged by size:
3079 988 972 390 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 2328 3546 2328
Size: 1218 4738
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4134
Fragments arranged by size:
4134 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2703 3914 4273 4432 518
Size: 648 1537 1211 359 159 2042
Fragments arranged by size:
2042 1537 1211 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3372 4049 5197 5442 1606
Size: 1766 677 1148 245 2120
Fragments arranged by size:
2120 1766 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3201 513
Size: 552 939 1197 3268
Fragments arranged by size:
3268 1197 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3314 4859 1132
Size: 97 2085 1545 2229
Fragments arranged by size:
2229 2085 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5956
PfoI T'CCnGG_A
Cuts at: 2022 2076 2470 2022
Size: 54 394 5508
Fragments arranged by size:
5508 394 54
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2211 4025 4057 5538 5570 2179
Size: 32 1814 32 1481 32 2565
Fragments arranged by size:
2565 1814 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2210 2210
Size: 5956
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PstI C_TGCA'G
Cuts at: 830 3308 830
Size: 2478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 3304 4439 5120 5311 826
Size: 219 2259 1135 681 191 1471
Fragments arranged by size:
2259 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3060 4060 4961 5223 820
Size: 197 926 1117 1000 901 262 1553
Fragments arranged by size:
1553 1117 1000 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2004 2336 3201 3395 513
Size: 552 939 332 865 194 3074
Fragments arranged by size:
3074 939 865 552 332 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2539 3983 4000 4153 4338 4783 325
Size: 1255 959 1444 17 153 185 445 1498
Fragments arranged by size:
1498 1444 1255 959 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2891 4202 5914
Size: 274 80 33 51 1443 914 1311 1712
Cuts at: 5914 96
Size: 138
Fragments arranged by size:
1712 1443 1311 914 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 2235 4085 2235
Size: 1850 4106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2011 3764 276
Size: 53 83 186 936 477 1753 2468
Fragments arranged by size:
2468 1753 936 477 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI