Restriction map for
pFN21AE5549
(Circular) (Six-base) MAPSORT of: pFN21AE5549.seq Check: 2710 from: 1 to: 5905
pFN21AE5549 5905 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 2820 4032 4405 1563
Size: 1257 1212 373 3063
Fragments arranged by size:
3063 1257 1212 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 2639 3968 5352 5620 1632
Size: 531 476 1329 1384 268 1917
Fragments arranged by size:
1917 1384 1329 531 476 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 3648 5220 3648
Size: 1572 4333
ApaI G_GGCC'C
Cuts at: 2029 2029
Size: 5905
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 2168 2404 3214 3440 3592 5701 1493
Size: 675 236 810 226 152 2109 1697
Fragments arranged by size:
2109 1697 810 675 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2138 4459 160
Size: 872 1106 2321 1606
Fragments arranged by size:
2321 1606 1106 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 1870
Size: 73 1287 4545
Fragments arranged by size:
4545 1287 73
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3226 4681 618
Size: 325 217 627 1439 1455 1842
Fragments arranged by size:
1842 1455 1439 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2029 2056 2425 3224 1391
Size: 638 27 369 799 4072
Fragments arranged by size:
4072 799 638 369 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2657 928
Size: 971 758 4176
Fragments arranged by size:
4176 971 758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BfrBI ATG'CAT
Cuts at: 2573 2573
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 2109 3322 2109
Size: 1213 4692
Bme1580I G_kGCm'C
Cuts at: 1790 2029 3966 5122 1790
Size: 239 1937 1156 2573
Fragments arranged by size:
2573 1937 1156 239
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2905 3636 4594
Size: 416 53 83 399 24 1488 731 958
Cuts at: 4594 442
Size: 1753
Fragments arranged by size:
1753 1488 958 731 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2630 4565 1239
Size: 1391 1935 2579
Fragments arranged by size:
2579 1935 1391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 4568 882
Size: 617 816 2253 2219
Fragments arranged by size:
2253 2219 816 617
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3712 4094 4729 275
Size: 53 83 186 936 2179 382 635 1451
Fragments arranged by size:
2179 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2883 4338 4736 5010 5157 5568 2883
Size: 1455 398 274 147 411 3220
Fragments arranged by size:
3220 1455 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5680
Fragments arranged by size:
5680 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5905
BseYI C'CCAG_C
Cuts at: 5108 5604 5108
Size: 496 5409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2742 1163
Size: 573 1006 4326
Fragments arranged by size:
4326 1006 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2023 3355 4116 5144 1540
Size: 275 186 22 1332 761 1028 2301
Fragments arranged by size:
2301 1332 1028 761 275 186 22
BsiHKAI G_wGCw'C
Cuts at: 2056 2425 3966 4051 5122 2056
Size: 369 1541 85 1071 2839
Fragments arranged by size:
2839 1541 1071 369 85
BsmI GAATG_Cn'
Cuts at: 2164 2686 3416 3509 2164
Size: 522 730 93 4560
Fragments arranged by size:
4560 730 522 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2056 2425 3224 3966 4051 5122
Size: 399 239 27 369 799 742 85 1071
Cuts at: 5122 1391
Size: 2174
Fragments arranged by size:
2174 1071 799 742 399 369 239 85
27
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2109 4400 4574 65
Size: 1183 861 2291 174 1396
Fragments arranged by size:
2291 1396 1183 861 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4549 4736 1700
Size: 110 2739 187 2869
Fragments arranged by size:
2869 2739 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 3235 3602 3987 4004 5456 5542 5554 5900
Size: 1263 367 385 17 1452 86 12 346
Cuts at: 5900 1972
Size: 1977
Fragments arranged by size:
1977 1452 1263 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 3003 3421 4215 4235 3003
Size: 418 794 20 4673
Fragments arranged by size:
4673 794 418 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DraIII CAC_nnn'GTG
Cuts at: 2471 2471
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3352 4241 5870
Size: 54 1425 50 275 18 1522 889 1629
Cuts at: 5870 8
Size: 43
Fragments arranged by size:
1629 1522 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3835 1353
Size: 2482 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2471 4492 4866 5012 387
Size: 1064 138 882 2021 374 146 1280
Fragments arranged by size:
2021 1280 1064 882 374 146 138
EcoICRI GAG'CTC
Cuts at: 2054 2423 2054
Size: 369 5536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 2630 2639 3968 4565 5352 5620
Size: 393 531 467 9 1329 597 787 268
Cuts at: 5620 1239
Size: 1524
Fragments arranged by size:
1524 1329 787 597 531 467 393 268
9
EcoO109I rG'GnC_Cy
Cuts at: 2026 2043 2026
Size: 17 5888
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3062 3062
Size: 5905
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 5052 1678
Size: 3374 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373
Size: 32 135 32 2974 32 42 32 2626
Fragments arranged by size:
2974 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2742 3249 3495 669
Size: 988 281 804 507 246 3079
Fragments arranged by size:
3079 988 804 507 281 246
HindIII A'AGCT_T
Cuts at: 748 2870 3159 748
Size: 2122 289 3494
Fragments arranged by size:
3494 2122 289
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 2486 3504 2486
Size: 1018 4887
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2486 5019 5203 1305
Size: 684 497 2533 184 2007
Fragments arranged by size:
2533 2007 684 497 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4083
Fragments arranged by size:
4083 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3863 4222 4381 518
Size: 648 2697 359 159 2042
Fragments arranged by size:
2697 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3011 3321 3998 5146 5391 1606
Size: 1405 310 677 1148 245 2120
Fragments arranged by size:
2120 1405 1148 677 310 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5353
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NsiI A_TGCA'T
Cuts at: 2575 2575
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 3263 4808 1132
Size: 97 2034 1545 2229
Fragments arranged by size:
2229 2034 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2471 1108
Size: 1363 4542
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2025 2025
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2843 3010 3257 830
Size: 2013 167 247 3478
Fragments arranged by size:
3478 2013 247 167
PvuI CG_AT'CG
Cuts at: 2001 2023 2001
Size: 22 5883
PvuII CAG'CTG
Cuts at: 3011 3011
Size: 5905
SacI G_AGCT'C
Cuts at: 2056 2425 2056
Size: 369 5536
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2839 3006 3253 4388 5069 5260 826
Size: 219 1794 167 247 1135 681 191 1471
Fragments arranged by size:
1794 1471 1135 681 247 219 191 167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 3344 513
Size: 552 2279 3074
Fragments arranged by size:
3074 2279 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3932 3949 4102 4287 4732 325
Size: 1255 2352 17 153 185 445 1498
Fragments arranged by size:
2352 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2578 4151 5863
Size: 274 80 33 51 1443 601 1573 1712
Cuts at: 5863 96
Size: 138
Fragments arranged by size:
1712 1573 1443 601 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 2250 2802 4034 2250
Size: 552 1232 4121
Fragments arranged by size:
4121 1232 552
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI