Restriction map for
pFN21AE5547
(Circular) (Six-base) MAPSORT of: pFN21AE5547.seq Check: 9897 from: 1 to: 5902
pFN21AE5547 5902 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3193 3193
Size: 5902
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3000 3245 1937
Size: 1063 245 4594
Fragments arranged by size:
4594 1063 245
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 2525 3965 5349 5617 1632
Size: 747 146 1440 1384 268 1917
Fragments arranged by size:
1917 1440 1384 747 268 146
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 2836 4801 5778 1203
Size: 1633 1965 977 1327
Fragments arranged by size:
1965 1633 1327 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 2839 1166
Size: 1673 4229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 2098 3645 5217 2098
Size: 1547 1572 2783
Fragments arranged by size:
2783 1572 1547
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 2451 3211 3437 3589 5698 1493
Size: 958 760 226 152 2109 1697
Fragments arranged by size:
2109 1697 958 760 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 3227 1870
Size: 73 118 1166 4545
Fragments arranged by size:
4545 1166 118 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3181 3223 4678 618
Size: 325 217 627 1394 42 1455 1842
Fragments arranged by size:
1842 1455 1394 627 325 217 42
BanII G_rGCy'C
Cuts at: 1391 3221 1391
Size: 1830 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BfrBI ATG'CAT
Cuts at: 2695 2695
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 3963 5119 1790
Size: 2173 1156 2573
Fragments arranged by size:
2573 2173 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2275 2772 3633
Size: 416 53 83 399 24 858 497 861
Cuts at: 3633 4591 442
Size: 958 1753
Fragments arranged by size:
1753 958 861 858 497 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3167 4562 1239
Size: 1928 1395 2579
Fragments arranged by size:
2579 1928 1395
Bpu10I CC'TnA_GC
Cuts at: 3158 3158
Size: 5902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2305 4027 4892 5190 2305
Size: 1722 865 298 3017
Fragments arranged by size:
3017 1722 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2837 3067 3693 493
Size: 711 1633 230 626 2702
Fragments arranged by size:
2702 1633 711 626 230
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3709 4091 4726 275
Size: 53 83 186 936 2176 382 635 1451
Fragments arranged by size:
2176 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4335 4733 5007 5154 5565 4335
Size: 398 274 147 411 4672
Fragments arranged by size:
4672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 1178
Size: 30 165 30 789 30 4858
Fragments arranged by size:
4858 789 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 1186
Size: 1132 4770
BseYI C'CCAG_C
Cuts at: 2867 3086 5105 5601 2867
Size: 219 2019 496 3168
Fragments arranged by size:
3168 2019 496 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2089 1163
Size: 573 353 4976
Fragments arranged by size:
4976 573 353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3963 4048 5119 3963
Size: 85 1071 4746
Fragments arranged by size:
4746 1071 85
BsmI GAATG_Cn'
Cuts at: 2695 2768 3017 3413 3506 2695
Size: 73 249 396 93 5091
Fragments arranged by size:
5091 396 249 93 73
BsmBI CGTCTCn'nnnn_
Cuts at: 3185 3185
Size: 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3221 3963 4048 5119 1391
Size: 399 1431 742 85 1071 2174
Fragments arranged by size:
2174 1431 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3193 3257 844
Size: 2349 64 3489
Fragments arranged by size:
3489 2349 64
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2055 3120 4397 4571 65
Size: 1183 807 1065 1277 174 1396
Fragments arranged by size:
1396 1277 1183 1065 807 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2753 4546 4733 1700
Size: 110 943 1793 187 2869
Fragments arranged by size:
2869 1793 943 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 2625 3962 4974 2625
Size: 1337 1012 3553
Fragments arranged by size:
3553 1337 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2629 3131 3232 3599 3984 4001 5453 5539
Size: 657 502 101 367 385 17 1452 86
Cuts at: 5539 5551 5897 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 657 502 385 367 346 101
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2642 2642
Size: 5902
BtgI C'CryG_G
Cuts at: 513 1065 2982 513
Size: 552 1917 3433
Fragments arranged by size:
3433 1917 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 2737 3418 4212 4232 2737
Size: 681 794 20 4407
Fragments arranged by size:
4407 794 681 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3349 4238 5867
Size: 54 1425 50 275 18 1519 889 1629
Cuts at: 5867 8
Size: 43
Fragments arranged by size:
1629 1519 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 3832 1353
Size: 740 1739 3423
Fragments arranged by size:
3423 1739 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2525 3167 3965 4562 5349 5617
Size: 393 747 146 642 798 597 787 268
Cuts at: 5617 1239
Size: 1524
Fragments arranged by size:
1524 798 787 747 642 597 393 268
146
EcoO109I rG'GnC_Cy
Cuts at: 2668 2668
Size: 5902
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
EcoRV GAT'ATC
Cuts at: 2014 2014
Size: 5902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 5902
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 5049 1678
Size: 3371 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 4543 4575 4617
Size: 32 135 32 923 32 2016 32 42
Cuts at: 4617 4649 1373
Size: 32 2626
Fragments arranged by size:
2626 2016 923 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 2911 3246 3492 669
Size: 988 281 208 765 335 246 3079
Fragments arranged by size:
3079 988 765 335 281 246 208
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2538 5016 5200 1305
Size: 1233 2478 184 2007
Fragments arranged by size:
2478 2007 1233 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4080
Fragments arranged by size:
4080 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2385 2839 2944 3860 4219 4378 518
Size: 648 1219 454 105 916 359 159 2042
Fragments arranged by size:
2042 1219 916 648 454 359 159 105
MspA1I CmG'CkG
Cuts at: 1606 2095 2867 3318 3995 5143 5388 1606
Size: 489 772 451 677 1148 245 2120
Fragments arranged by size:
2120 1148 772 677 489 451 245
NcoI C'CATG_G
Cuts at: 513 1065 2982 513
Size: 552 1917 3433
Fragments arranged by size:
3433 1917 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NsiI A_TGCA'T
Cuts at: 2697 2697
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 2695 3260 4805 1132
Size: 97 1466 565 1545 2229
Fragments arranged by size:
2229 1545 1466 565 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3178 1108
Size: 2070 3832
PfoI T'CCnGG_A
Cuts at: 2019 3134 2019
Size: 1115 4787
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PmlI CAC'GTG
Cuts at: 2837 3067 2837
Size: 230 5672
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2278 2310 3971 4003 5484 5516 2278
Size: 32 1661 32 1481 32 2664
Fragments arranged by size:
2664 1661 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 2186 2880 3472 2186
Size: 694 592 4616
Fragments arranged by size:
4616 694 592
PstI C_TGCA'G
Cuts at: 830 3190 3254 830
Size: 2360 64 3478
Fragments arranged by size:
3478 2360 64
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
PvuII CAG'CTG
Cuts at: 2095 2867 2095
Size: 772 5130
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2702 3186 3250 4385 5066 5257 826
Size: 219 1657 484 64 1135 681 191 1471
Fragments arranged by size:
1657 1471 1135 681 484 219 191 64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 2063 3229 1872
Size: 191 1166 4545
Fragments arranged by size:
4545 1166 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2284 4006 4907 5169 820
Size: 197 926 341 1722 901 262 1553
Fragments arranged by size:
1722 1553 926 901 341 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SrfI GCCC'GGGC
Cuts at: 2063 2063
Size: 5902
SspI AAT'ATT
Cuts at: 5 52 2230 3826 5
Size: 47 2178 1596 2081
Fragments arranged by size:
2178 2081 1596 47
StuI AGG'CCT
Cuts at: 1517 2904 1517
Size: 1387 4515
StyI C'CwwG_G
Cuts at: 513 1065 2899 2982 3341 513
Size: 552 1834 83 359 3074
Fragments arranged by size:
3074 1834 552 359 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2446 2975 3929 3946 4099 4284 4729
Size: 1255 866 529 954 17 153 185 445
Cuts at: 4729 325
Size: 1498
Fragments arranged by size:
1498 1255 954 866 529 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2167 2798 4148
Size: 274 80 33 51 1443 190 631 1350
Cuts at: 4148 5860 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1350 631 274 190 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2442 2474 5862 5894 2442
Size: 32 3388 32 2450
Fragments arranged by size:
3388 2450 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 2717 3238 2717
Size: 521 5381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3142 3142
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 2061 3227 1870
Size: 191 1166 4545
Fragments arranged by size:
4545 1166 191
XmnI GAAnn'nnTTC
Cuts at: 4031 4031
Size: 5902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SwaI