Restriction map for
pFN21AE5540
(Circular) (Six-base) MAPSORT of: pFN21AE5540.seq Check: 9305 from: 1 to: 5788
pFN21AE5540 5788 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2903 3851 5235 5503 1632
Size: 1271 948 1384 268 1917
Fragments arranged by size:
1917 1384 1271 948 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 2044 2123 2237 2365 2722 2794 2989 3067 3531
Size: 79 114 128 357 72 195 78 464
Cuts at: 3531 5103 2044
Size: 1572 2729
Fragments arranged by size:
2729 1572 464 357 195 128 114 79
78 72
ApaI G_GGCC'C
Cuts at: 2133 2133
Size: 5788
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 2165 3097 3323 3475 5584 1493
Size: 672 932 226 152 2109 1697
Fragments arranged by size:
2109 1697 932 672 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2388 2591 3113 1870
Size: 73 445 203 522 4545
Fragments arranged by size:
4545 522 445 203 73
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3109 4564 618
Size: 325 217 627 1322 1455 1842
Fragments arranged by size:
1842 1455 1322 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2133 3037 3107 1391
Size: 742 904 70 4072
Fragments arranged by size:
4072 904 742 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2631 928
Size: 971 732 4085
Fragments arranged by size:
4085 971 732
BbvCI CC'TCA_GC
Cuts at: 2517 2517
Size: 5788
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2240 2513 2531
Size: 107 122 71 631 605 568 273 18
Cuts at: 2531 2593 2642 4399 136
Size: 62 49 1757 1525
Fragments arranged by size:
1757 1525 631 605 568 273 122 107
71 62 49 18
BglII A'GATC_T
Cuts at: 2055 5783 2055
Size: 3728 2060
BlpI GC'TnA_GC
Cuts at: 2193 2290 3205 2193
Size: 97 915 4776
Fragments arranged by size:
4776 915 97
Bme1580I G_kGCm'C
Cuts at: 1790 2133 3849 5005 1790
Size: 343 1716 1156 2573
Fragments arranged by size:
2573 1716 1156 343
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 4477 442
Size: 416 53 83 399 24 2102 958 1753
Fragments arranged by size:
2102 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2211 2744 2816 3089 4448 1239
Size: 972 533 72 273 1359 2579
Fragments arranged by size:
2579 1359 972 533 273 72
Bpu10I CC'TnA_GC
Cuts at: 2517 2517
Size: 5788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2094 3913 4778 5076 2094
Size: 1819 865 298 2806
Fragments arranged by size:
2806 1819 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4451 882
Size: 617 2952 2219
Fragments arranged by size:
2952 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 3595 3977 4612
Size: 53 83 186 936 495 1567 382 635
Cuts at: 4612 275
Size: 1451
Fragments arranged by size:
1567 1451 936 635 495 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2169 2463 4221 4619 4893 5040 5451 2169
Size: 294 1758 398 274 147 411 2506
Fragments arranged by size:
2506 1758 411 398 294 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5563
Fragments arranged by size:
5563 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2534 2813 1186
Size: 1348 279 4161
Fragments arranged by size:
4161 1348 279
BseYI C'CCAG_C
Cuts at: 4991 5487 4991
Size: 496 5292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2554 1163
Size: 573 818 4397
Fragments arranged by size:
4397 818 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2940 3238 3999 5027 1540
Size: 275 186 939 298 761 1028 2301
Fragments arranged by size:
2301 1028 939 761 298 275 186
BsiHKAI G_wGCw'C
Cuts at: 2199 3849 3934 5005 2199
Size: 1650 85 1071 2982
Fragments arranged by size:
2982 1650 1071 85
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2199 3037 3107 3849 3934 5005
Size: 399 343 66 838 70 742 85 1071
Cuts at: 5005 1391
Size: 2174
Fragments arranged by size:
2174 1071 838 742 399 343 85 70
66
BspEI T'CCGG_A
Cuts at: 2169 5451 2169
Size: 3282 2506
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2043 2997 3143 844
Size: 1199 954 146 3489
Fragments arranged by size:
3489 1199 954 146
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4283 4457 65
Size: 1183 3035 174 1396
Fragments arranged by size:
3035 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2632 2712 2748 2919 2939 2973 4432
Size: 110 822 80 36 171 20 34 1459
Cuts at: 4432 4619 1700
Size: 187 2869
Fragments arranged by size:
2869 1459 822 187 171 110 80 36
34 20
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 2087 3073 1925
Size: 162 986 4640
Fragments arranged by size:
4640 986 162
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2989 794
Size: 2195 3593
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2212 2212
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2055 2376 2847 3118 3485 3870 3887 5339
Size: 83 321 471 271 367 385 17 1452
Cuts at: 5339 5425 5437 5783 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 471 385 367 346 321 271
86 83 17 12
Bsu36I CC'TnA_GG
Cuts at: 2253 2253
Size: 5788
BtgI C'CryG_G
Cuts at: 513 1065 2004 2526 2579 513
Size: 552 939 522 53 3722
Fragments arranged by size:
3722 939 552 522 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 2407 3304 4098 4118 2407
Size: 897 794 20 4077
Fragments arranged by size:
4077 897 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2316 2634
Size: 54 1425 50 275 18 216 270 318
Cuts at: 2634 2767 2937 3235 4124 5753 8
Size: 133 170 298 889 1629 43
Fragments arranged by size:
1629 1425 889 318 298 275 270 216
170 133 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2937 3235 1537
Size: 275 1125 298 4090
Fragments arranged by size:
4090 1125 298 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2175 2465 2657 3718 1353
Size: 822 290 192 1061 3423
Fragments arranged by size:
3423 1061 822 290 192
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2458 2849 4375 4749 4895 387
Size: 1064 138 869 391 1526 374 146 1280
Fragments arranged by size:
1526 1280 1064 869 391 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2744 2816 2903 3089 3851 4448
Size: 393 579 533 72 87 186 762 597
Cuts at: 4448 5235 5503 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 762 597 579 533 393 268
186 87 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2828 2870 3059 2828
Size: 42 189 5557
Fragments arranged by size:
5557 189 42
EcoO109I rG'GnC_Cy
Cuts at: 2129 2130 2565 2878 3064 2129
Size: 1 435 313 186 4853
Fragments arranged by size:
4853 435 313 186 1
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FspI TGC'GCA
Cuts at: 2993 4294 2993
Size: 1301 4487
HaeII r_GCGC'y
Cuts at: 1678 2731 2920 2968 3022 4935 1678
Size: 1053 189 48 54 1913 2531
Fragments arranged by size:
2531 1913 1053 189 54 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 4429 4461 4503
Size: 32 135 32 743 32 2082 32 42
Cuts at: 4503 4535 1373
Size: 32 2626
Fragments arranged by size:
2626 2082 743 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4902 5086 1305
Size: 3597 184 2007
Fragments arranged by size:
3597 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 2318 10
Size: 54 1425 343 216 270 3480
Fragments arranged by size:
3480 1425 343 270 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3746 4105 4264 518
Size: 648 2580 359 159 2042
Fragments arranged by size:
2580 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2581 2695 2719 2911 3204 3881 5029 5274
Size: 975 114 24 192 293 677 1148 245
Cuts at: 5274 1606
Size: 2120
Fragments arranged by size:
2120 1148 975 677 293 245 192 114
24
NaeI GCC'GGC
Cuts at: 2634 2750 2941 2634
Size: 116 191 5481
Fragments arranged by size:
5481 191 116
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4297
Fragments arranged by size:
4297 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NgoMIV G'CCGG_C
Cuts at: 2632 2748 2939 2632
Size: 116 191 5481
Fragments arranged by size:
5481 191 116
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2542 1108
Size: 1434 4354
PfoI T'CCnGG_A
Cuts at: 2379 2379
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3064 3064
Size: 5788
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2129 2129
Size: 5788
PstI C_TGCA'G
Cuts at: 830 2040 2128 2308 2736 2874 3140 830
Size: 1210 88 180 428 138 266 3478
Fragments arranged by size:
3478 1210 428 266 180 138 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2719 2719
Size: 5788
SacII CC_GC'GG
Cuts at: 2582 2582
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 2736 3140 2736
Size: 404 5384
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 2036 2124 2304 2732 2870 3136 4271
Size: 219 991 88 180 428 138 266 1135
Cuts at: 4271 4952 5143 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 991 681 428 266 219 191
180 138 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 2531 2531
Size: 5788
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 2919 1700
Size: 1219 4569
SmaI CCC'GGG
Cuts at: 1872 2390 2593 3115 1872
Size: 518 203 522 4545
Fragments arranged by size:
4545 522 518 203
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2109 3892 4793 5055 820
Size: 197 926 166 1783 901 262 1553
Fragments arranged by size:
1783 1553 926 901 262 197 166
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SrfI GCCC'GGGC
Cuts at: 2593 2593
Size: 5788
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 2251 3081 1517
Size: 734 830 4224
Fragments arranged by size:
4224 830 734
StyI C'CwwG_G
Cuts at: 513 1065 2004 2022 2211 2406 2427 3227 513
Size: 552 939 18 189 195 21 800 3074
Fragments arranged by size:
3074 939 800 552 195 189 21 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3815 3832 3985 4170 4615 325
Size: 1255 2235 17 153 185 445 1498
Fragments arranged by size:
2235 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4034 5746 96
Size: 274 80 33 51 1443 2057 1712 138
Fragments arranged by size:
2057 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5748 5780 5748
Size: 32 5756
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 2388 2591 3113 1870
Size: 518 203 522 4545
Fragments arranged by size:
4545 522 518 203
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfoI
SwaI XcmI