Restriction map for
pFN21AE5533
(Circular) (Six-base) MAPSORT of: pFN21AE5533.seq Check: 2336 from: 1 to: 6106
pFN21AE5533 6106 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4169 5553 5821 1632
Size: 2537 1384 268 1917
Fragments arranged by size:
2537 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 5005 5982 1203
Size: 3802 977 1327
Fragments arranged by size:
3802 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 2902 4835 2902
Size: 1933 4173
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6106
AlwNI CAG_nnn'CTG
Cuts at: 3849 5421 3849
Size: 1572 4534
ApaI G_GGCC'C
Cuts at: 2475 3021 2475
Size: 546 5560
ApaLI G'TGCA_C
Cuts at: 4163 5319 4163
Size: 1156 4950
ApoI r'AATT_y
Cuts at: 1493 2267 2333 3415 3641 3793 5902 1493
Size: 774 66 1082 226 152 2109 1697
Fragments arranged by size:
2109 1697 1082 774 226 152 66
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 3431 1870
Size: 73 1488 4545
Fragments arranged by size:
4545 1488 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2243 2276 2243
Size: 33 6073
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2378 3032 3427 4882 618
Size: 325 217 627 591 654 395 1455 1842
Fragments arranged by size:
1842 1455 654 627 591 395 325 217
BanII G_rGCy'C
Cuts at: 1391 2022 2475 2643 3021 3385 3425 1391
Size: 631 453 168 378 364 40 4072
Fragments arranged by size:
4072 631 453 378 364 168 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4297 4331 1903
Size: 34 50 34 2276 34 3678
Fragments arranged by size:
3678 2276 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4717 136
Size: 107 122 71 631 605 3045 1525
Fragments arranged by size:
3045 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6101 6101
Size: 6106
BlpI GC'TnA_GC
Cuts at: 3523 3523
Size: 6106
Bme1580I G_kGCm'C
Cuts at: 1790 2084 2205 2475 2771 3021 4167 5323 1790
Size: 294 121 270 296 250 1146 1156 2573
Fragments arranged by size:
2573 1156 1146 296 294 270 250 121
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2194 2753 3287
Size: 416 53 83 399 24 777 559 534
Cuts at: 3287 3837 4795 442
Size: 550 958 1753
Fragments arranged by size:
1753 958 777 559 550 534 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4766 1239
Size: 3527 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2519 2600 4231 5096 5394 2519
Size: 81 1631 865 298 3231
Fragments arranged by size:
3231 1631 865 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4769 882
Size: 617 3270 2219
Fragments arranged by size:
3270 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 493
Size: 711 2693 2702
Fragments arranged by size:
2702 2693 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2130 3913 4295 4930
Size: 53 83 186 936 597 1783 382 635
Cuts at: 4930 275
Size: 1451
Fragments arranged by size:
1783 1451 936 635 597 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2704 4539 4937 5211 5358 5769 2704
Size: 1835 398 274 147 411 3041
Fragments arranged by size:
3041 1835 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5881
Fragments arranged by size:
5881 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2712 3068 1186
Size: 1526 356 4224
Fragments arranged by size:
4224 1526 356
BseYI C'CCAG_C
Cuts at: 2204 3378 5309 5805 2204
Size: 1174 1931 496 2505
Fragments arranged by size:
2505 1931 1174 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5533
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3556 4317 5345 1540
Size: 275 186 1555 761 1028 2301
Fragments arranged by size:
2301 1555 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2643 2704 4167 4252 5323 2022
Size: 621 61 1463 85 1071 2805
Fragments arranged by size:
2805 1463 1071 621 85 61
BsmI GAATG_Cn'
Cuts at: 2461 3617 3710 2461
Size: 1156 93 4857
Fragments arranged by size:
4857 1156 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2084 2205 2475 2643 2704 2771
Size: 399 232 62 121 270 168 61 67
Cuts at: 2771 3021 3385 3425 4167 4252 5323 1391
Size: 250 364 40 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 364 270 250 232
168 121 85 67 62 61 40
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 2410 3995 5725 2410
Size: 1585 1730 2791
Fragments arranged by size:
2791 1730 1585
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2535 2840 3461 844
Size: 1691 305 621 3489
Fragments arranged by size:
3489 1691 621 305
BsrBI CCG'CTC
Cuts at: 1394 1823 2572 3993 1394
Size: 429 749 1421 3507
Fragments arranged by size:
3507 1421 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2630 4601 4775 65
Size: 1183 1382 1971 174 1396
Fragments arranged by size:
1971 1396 1382 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4750 4937 1700
Size: 110 2940 187 2869
Fragments arranged by size:
2940 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 4166 5178 4166
Size: 1012 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6106
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstXI CCAn_nnnn'nTGG
Cuts at: 2874 3156 3300 2874
Size: 282 144 5680
Fragments arranged by size:
5680 282 144
BstYI r'GATC_y
Cuts at: 1972 3436 3803 4188 4205 5657 5743 5755 6101
Size: 1464 367 385 17 1452 86 12 346
Cuts at: 6101 1972
Size: 1977
Fragments arranged by size:
1977 1464 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2670 2670
Size: 6106
BtgI C'CryG_G
Cuts at: 513 1065 3283 513
Size: 552 2218 3336
Fragments arranged by size:
3336 2218 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5367
Fragments arranged by size:
5367 683 56
BtsI GCAGTG_nn'
Cuts at: 2842 3622 4416 4436 2842
Size: 780 794 20 4512
Fragments arranged by size:
4512 794 780 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3235 3553 4442
Size: 54 1425 50 275 18 1405 318 889
Cuts at: 4442 6071 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1405 889 318 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2579 4036 1353
Size: 1226 1457 3423
Fragments arranged by size:
3423 1457 1226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4693 5067 5213 387
Size: 1064 138 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2020 2641 2020
Size: 621 5485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4169 4766 5553 5821 1239
Size: 393 2537 597 787 268 1524
Fragments arranged by size:
2537 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2471 3075 2471
Size: 604 5502
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2097 5253 1678
Size: 419 3156 2531
Fragments arranged by size:
3156 2531 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2573 2605 2888 2920 4747
Size: 32 135 32 1001 32 283 32 1827
Cuts at: 4747 4779 4821 4853 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1827 1001 283 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3450 3696 669
Size: 988 281 1512 246 3079
Fragments arranged by size:
3079 1512 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 2949 3705 2949
Size: 756 5350
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2664 5220 5404 1305
Size: 1359 2556 184 2007
Fragments arranged by size:
2556 2007 1359 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3237 10
Size: 54 1425 343 1405 2879
Fragments arranged by size:
2879 1425 1405 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2872 4064 4423 4582 518
Size: 648 1706 1192 359 159 2042
Fragments arranged by size:
2042 1706 1192 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2140 3226 3522 4199 5347 5592 1606
Size: 534 1086 296 677 1148 245 2120
Fragments arranged by size:
2120 1148 1086 677 534 296 245
NcoI C'CATG_G
Cuts at: 513 1065 3283 513
Size: 552 2218 3336
Fragments arranged by size:
3336 2218 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 3464 5009 1132
Size: 97 2235 1545 2229
Fragments arranged by size:
2235 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6106
PciI A'CATG_T
Cuts at: 5005 5005
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2352 1108
Size: 1244 4862
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3075 3075
Size: 6106
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6106
PsiI TTA'TAA
Cuts at: 2847 3676 2847
Size: 829 5277
PspOMI G'GGCC_C
Cuts at: 2471 3017 2471
Size: 546 5560
PstI C_TGCA'G
Cuts at: 830 2837 3458 830
Size: 2007 621 3478
Fragments arranged by size:
3478 2007 621
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
PvuII CAG'CTG
Cuts at: 2140 3226 2140
Size: 1086 5020
SacI G_AGCT'C
Cuts at: 2022 2643 2022
Size: 621 5485
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SapI GCTCTTCn'nnn_
Cuts at: 2579 2579
Size: 6106
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SfcI C'TryA_G
Cuts at: 826 1045 2833 2914 3042 3454 4589 5270 5461
Size: 219 1788 81 128 412 1135 681 191
Cuts at: 5461 826
Size: 1471
Fragments arranged by size:
1788 1471 1135 681 412 219 191 128
81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3433 1872
Size: 1561 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2498 2579 4210 5111 5373 820
Size: 197 926 555 81 1631 901 262 1553
Fragments arranged by size:
1631 1553 926 901 555 262 197 81
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 3464 1132
Size: 2332 3774
SspI AAT'ATT
Cuts at: 5 52 2441 3013 4030 5
Size: 47 2389 572 1017 2081
Fragments arranged by size:
2389 2081 1017 572 47
StuI AGG'CCT
Cuts at: 1517 2372 1517
Size: 855 5251
StyI C'CwwG_G
Cuts at: 513 1065 3232 3283 3545 513
Size: 552 2167 51 262 3074
Fragments arranged by size:
3074 2167 552 262 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4133 4150 4303 4488 4933 325
Size: 1255 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2541 4352 6064
Size: 274 80 33 51 1443 564 1811 1712
Cuts at: 6064 96
Size: 138
Fragments arranged by size:
1811 1712 1443 564 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6066 6098 6066
Size: 32 6074
Tth111I GACn'n_nGTC
Cuts at: 1316 2486 3140 1316
Size: 1170 654 4282
Fragments arranged by size:
4282 1170 654
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3250 3250
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 3431 1870
Size: 1561 4545
XmnI GAAnn'nnTTC
Cuts at: 2562 4235 2562
Size: 1673 4433
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI