Restriction map for
pFN21AE5532
(Circular) (Six-base) MAPSORT of: pFN21AE5532.seq Check: 4869 from: 1 to: 5884
pFN21AE5532 5884 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3085 3085
Size: 5884
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 2999 3104 3947 5331 5599 1632
Size: 731 636 105 843 1384 268 1917
Fragments arranged by size:
1917 1384 843 731 636 268 105
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 4783 5760 1203
Size: 3580 977 1327
Fragments arranged by size:
3580 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 2866 4613 2866
Size: 1747 4137
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5884
AlwNI CAG_nnn'CTG
Cuts at: 2205 2857 2977 3160 3627 5199 2205
Size: 652 120 183 467 1572 2890
Fragments arranged by size:
2890 1572 652 467 183 120
ApaLI G'TGCA_C
Cuts at: 3941 5097 3941
Size: 1156 4728
ApoI r'AATT_y
Cuts at: 1493 3193 3419 3571 5680 1493
Size: 1700 226 152 2109 1697
Fragments arranged by size:
2109 1700 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 2220 2702 3114 3209 1870
Size: 73 277 482 412 95 4545
Fragments arranged by size:
4545 482 412 277 95 73
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3205 4660 618
Size: 325 217 627 1418 1455 1842
Fragments arranged by size:
1842 1455 1418 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2901 3203 1391
Size: 1510 302 4072
Fragments arranged by size:
4072 1510 302
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3061 928
Size: 971 1162 3751
Fragments arranged by size:
3751 1162 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4075 4109 1903
Size: 34 50 34 2054 34 3678
Fragments arranged by size:
3678 2054 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3776 4986 3776
Size: 1210 4674
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4495 136
Size: 107 122 71 631 605 2823 1525
Fragments arranged by size:
2823 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2319 5879 2319
Size: 3560 2324
BlpI GC'TnA_GC
Cuts at: 2522 2597 3301 2522
Size: 75 704 5105
Fragments arranged by size:
5105 704 75
Bme1580I G_kGCm'C
Cuts at: 1790 3945 5101 1790
Size: 2155 1156 2573
Fragments arranged by size:
2573 2155 1156
BmgBI CAC'GTC
Cuts at: 1318 2351 2389 3064 1318
Size: 1033 38 675 4138
Fragments arranged by size:
4138 1033 675 38
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2258 3615 4573
Size: 416 53 83 399 24 841 1357 958
Cuts at: 4573 442
Size: 1753
Fragments arranged by size:
1753 1357 958 841 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 3125 4544 1239
Size: 890 996 1419 2579
Fragments arranged by size:
2579 1419 996 890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 4009 4874 5172 2210
Size: 1799 865 298 2922
Fragments arranged by size:
2922 1799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3139 4547 882
Size: 617 1640 1408 2219
Fragments arranged by size:
2219 1640 1408 617
BsaAI yAC'GTr
Cuts at: 493 1204 3675 493
Size: 711 2471 2702
Fragments arranged by size:
2702 2471 711
BsaBI GATnn'nnATC
Cuts at: 5878 5878
Size: 5884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3691 4073 4708 275
Size: 53 83 186 936 2158 382 635 1451
Fragments arranged by size:
2158 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2484 4317 4715 4989 5136 5547 2484
Size: 1833 398 274 147 411 2821
Fragments arranged by size:
2821 1833 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2912 2942 1178
Size: 30 165 30 1509 30 4120
Fragments arranged by size:
4120 1509 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 2531 1186
Size: 829 516 4539
Fragments arranged by size:
4539 829 516
BseYI C'CCAG_C
Cuts at: 2237 2716 3121 3148 5087 5583 2237
Size: 479 405 27 1939 496 2538
Fragments arranged by size:
2538 1939 496 479 405 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 2636 1163
Size: 573 684 216 4411
Fragments arranged by size:
4411 684 573 216
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3334 4095 5123 1540
Size: 275 186 1333 761 1028 2301
Fragments arranged by size:
2301 1333 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3027 3945 4030 5101 3027
Size: 918 85 1071 3810
Fragments arranged by size:
3810 1071 918 85
BsmI GAATG_Cn'
Cuts at: 3395 3488 3395
Size: 93 5791
BsmBI CGTCTCn'nnnn_
Cuts at: 2341 2341
Size: 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2901 3027 3203 3945 4030 5101 1391
Size: 399 1111 126 176 742 85 1071 2174
Fragments arranged by size:
2174 1111 1071 742 399 176 126 85
BspEI T'CCGG_A
Cuts at: 2484 5547 2484
Size: 3063 2821
BspHI T'CATG_A
Cuts at: 2778 3773 5503 2778
Size: 995 1730 3159
Fragments arranged by size:
3159 1730 995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3085 3186 3239 844
Size: 2241 101 53 3489
Fragments arranged by size:
3489 2241 101 53
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4379 4553 65
Size: 1183 3131 174 1396
Fragments arranged by size:
3131 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2134 4528 4715 1700
Size: 110 324 2394 187 2869
Fragments arranged by size:
2869 2394 324 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 3944 4956 3944
Size: 1012 4872
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2205 2443 794
Size: 1411 238 4235
Fragments arranged by size:
4235 1411 238
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5884
BstYI r'GATC_y
Cuts at: 1972 2319 3214 3581 3966 3983 5435 5521 5533
Size: 347 895 367 385 17 1452 86 12
Cuts at: 5533 5879 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 895 385 367 347 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2264 2275 2532 513
Size: 552 1199 11 257 3865
Fragments arranged by size:
3865 1199 552 257 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2180 487
Size: 683 56 954 4191
Fragments arranged by size:
4191 954 683 56
BtsI GCAGTG_nn'
Cuts at: 3058 3400 4194 4214 3058
Size: 342 794 20 4728
Fragments arranged by size:
4728 794 342 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DraIII CAC_nnn'GTG
Cuts at: 2389 2443 2389
Size: 54 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4891 809
Size: 507 3575 1802
Fragments arranged by size:
3575 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2353 2640 3331
Size: 54 1425 50 275 18 523 287 691
Cuts at: 3331 4220 5849 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 691 523 287 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3331 1537
Size: 275 1519 4090
Fragments arranged by size:
4090 1519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2108 2339 3038 3814 1353
Size: 668 87 231 699 776 3423
Fragments arranged by size:
3423 776 699 668 231 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4471 4845 4991 387
Size: 1064 138 2882 374 146 1280
Fragments arranged by size:
2882 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2363 2999 3104 3125 3947 4544
Size: 393 497 234 636 105 21 822 597
Cuts at: 4544 5331 5599 1239
Size: 787 268 1524
Fragments arranged by size:
1524 822 787 636 597 497 393 268
234 105 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2459 2459
Size: 5884
EcoO109I rG'GnC_Cy
Cuts at: 2431 2854 2991 3157 2431
Size: 423 137 166 5158
Fragments arranged by size:
5158 423 166 137
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FspI TGC'GCA
Cuts at: 4390 4390
Size: 5884
HaeII r_GCGC'y
Cuts at: 1678 5031 1678
Size: 3353 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2832 2852 2864 2884 4525
Size: 32 135 32 1260 20 12 20 1641
Cuts at: 4525 4557 4599 4631 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1641 1260 135 42 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3228 3474 669
Size: 988 281 1290 246 3079
Fragments arranged by size:
3079 1290 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5884
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4998 5182 1305
Size: 3693 184 2007
Fragments arranged by size:
3693 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4062
Fragments arranged by size:
4062 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2562 2624 3842 4201 4360 518
Size: 648 1396 62 1218 359 159 2042
Fragments arranged by size:
2042 1396 1218 648 359 159 62
MspA1I CmG'CkG
Cuts at: 1606 2341 2593 2716 2749 3300 3977 5125 5370
Size: 735 252 123 33 551 677 1148 245
Cuts at: 5370 1606
Size: 2120
Fragments arranged by size:
2120 1148 735 677 551 252 245 123
33
NcoI C'CATG_G
Cuts at: 513 1065 2264 2275 513
Size: 552 1199 11 4122
Fragments arranged by size:
4122 1199 552 11
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 2426 2629 2725 3242 4787 1132
Size: 97 1197 203 96 517 1545 2229
Fragments arranged by size:
2229 1545 1197 517 203 97 96
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5884
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2389 2857 3160 1108
Size: 1281 468 303 3832
Fragments arranged by size:
3832 1281 468 303
PfoI T'CCnGG_A
Cuts at: 2856 2856
Size: 5884
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2854 2854
Size: 5884
PshAI GACnn'nnGTC
Cuts at: 1613 2534 1613
Size: 921 4963
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PstI C_TGCA'G
Cuts at: 830 2048 2340 3096 3236 830
Size: 1218 292 756 140 3478
Fragments arranged by size:
3478 1218 756 292 140
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
PvuII CAG'CTG
Cuts at: 2341 2593 2716 2749 2341
Size: 252 123 33 5476
Fragments arranged by size:
5476 252 123 33
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SapI GCTCTTCn'nnn_
Cuts at: 3038 3038
Size: 5884
SbfI CC_TGCA'GG
Cuts at: 3096 3236 3096
Size: 140 5744
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SfcI C'TryA_G
Cuts at: 826 1045 2044 2336 2796 3092 3232 4367 5048
Size: 219 999 292 460 296 140 1135 681
Cuts at: 5048 5239 826
Size: 191 1471
Fragments arranged by size:
1471 1135 999 681 460 296 292 219
191 140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 2222 2704 3116 3211 1872
Size: 350 482 412 95 4545
Fragments arranged by size:
4545 482 412 350 95
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 3988 4889 5151 820
Size: 197 926 246 1799 901 262 1553
Fragments arranged by size:
1799 1553 926 901 262 246 197
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 2426 3242 1132
Size: 1294 816 3774
Fragments arranged by size:
3774 1294 816
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 3017 1517
Size: 1500 4384
StyI C'CwwG_G
Cuts at: 513 1065 2264 2275 2292 2395 2427 2931 3323
Size: 552 1199 11 17 103 32 504 392
Cuts at: 3323 513
Size: 3074
Fragments arranged by size:
3074 1199 552 504 392 103 32 17
11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2648 2723 3911 3928 4081 4266 4711
Size: 1255 1068 75 1188 17 153 185 445
Cuts at: 4711 325
Size: 1498
Fragments arranged by size:
1498 1255 1188 1068 445 185 153 75
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2245 3036 4130
Size: 274 80 33 51 1443 268 791 1094
Cuts at: 4130 5842 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1094 791 274 268 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2384 2416 5844 5876 2384
Size: 32 3428 32 2392
Fragments arranged by size:
3428 2392 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2561 2561
Size: 5884
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 2220 2702 3114 3209 1870
Size: 350 482 412 95 4545
Fragments arranged by size:
4545 482 412 350 95
XmnI GAAnn'nnTTC
Cuts at: 4013 4013
Size: 5884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI