Restriction map for
pFN21AE5530
(Circular) (Six-base) MAPSORT of: pFN21AE5530.seq Check: 7595 from: 1 to: 5995
pFN21AE5530 5995 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2270 2857 3338 1937
Size: 333 587 481 4594
Fragments arranged by size:
4594 587 481 333
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 4122 4495 1563
Size: 2559 373 3063
Fragments arranged by size:
3063 2559 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 2626 2997 4058 5442 5710 1632
Size: 467 527 371 1061 1384 268 1917
Fragments arranged by size:
1917 1384 1061 527 467 371 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 4894 5871 1203
Size: 3691 977 1327
Fragments arranged by size:
3691 1327 977
AgeI A'CCGG_T
Cuts at: 4826 4826
Size: 5995
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5995
AlwNI CAG_nnn'CTG
Cuts at: 2015 3738 5310 2015
Size: 1723 1572 2700
Fragments arranged by size:
2700 1723 1572
ApaLI G'TGCA_C
Cuts at: 4052 5208 4052
Size: 1156 4839
ApoI r'AATT_y
Cuts at: 1493 2228 2393 2481 2729 3159 3304 3530 3682
Size: 735 165 88 248 430 145 226 152
Cuts at: 3682 5791 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 735 430 248 226 165 152
145 88
AseI AT'TA_AT
Cuts at: 160 1032 2345 4549 160
Size: 872 1313 2204 1606
Fragments arranged by size:
2204 1606 1313 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 3320 1870
Size: 73 1377 4545
Fragments arranged by size:
4545 1377 73
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3316 4771 618
Size: 325 217 627 1529 1455 1842
Fragments arranged by size:
1842 1529 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3314 1391
Size: 1923 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2087 2345 2994 928
Size: 971 188 258 649 3929
Fragments arranged by size:
3929 971 649 258 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4186 4220 1903
Size: 34 2249 34 3678
Fragments arranged by size:
3678 2249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3887 5097 3887
Size: 1210 4785
BclI T'GATC_A
Cuts at: 1548 2362 1548
Size: 814 5181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4606 136
Size: 107 122 71 631 605 2934 1525
Fragments arranged by size:
2934 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3193 5990 3193
Size: 2797 3198
BlpI GC'TnA_GC
Cuts at: 3412 3412
Size: 5995
Bme1580I G_kGCm'C
Cuts at: 1790 4056 5212 1790
Size: 2266 1156 2573
Fragments arranged by size:
2573 2266 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3726 4684 442
Size: 416 53 83 399 24 2309 958 1753
Fragments arranged by size:
2309 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4655 1239
Size: 3416 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2451 4120 4985 5283 2451
Size: 1669 865 298 3163
Fragments arranged by size:
3163 1669 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4658 882
Size: 617 3159 2219
Fragments arranged by size:
3159 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3786 493
Size: 711 2582 2702
Fragments arranged by size:
2702 2582 711
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3802 4184 4819 275
Size: 53 83 186 936 2269 382 635 1451
Fragments arranged by size:
2269 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4428 4826 5100 5247 5658 4428
Size: 398 274 147 411 4765
Fragments arranged by size:
4765 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5770
Fragments arranged by size:
5770 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2838 1186
Size: 1652 4343
BseYI C'CCAG_C
Cuts at: 2249 5198 5694 2249
Size: 2949 496 2550
Fragments arranged by size:
2949 2550 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2868 1163
Size: 573 1132 4290
Fragments arranged by size:
4290 1132 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3445 4206 5234 1540
Size: 275 186 1444 761 1028 2301
Fragments arranged by size:
2301 1444 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4056 4141 5212 4056
Size: 85 1071 4839
Fragments arranged by size:
4839 1071 85
BsmI GAATG_Cn'
Cuts at: 2095 2710 2959 3506 3599 2095
Size: 615 249 547 93 4491
Fragments arranged by size:
4491 615 547 249 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3314 4056 4141 5212 1391
Size: 399 1524 742 85 1071 2174
Fragments arranged by size:
2174 1524 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 3884 5614 3884
Size: 1730 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3350 844
Size: 2506 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3882 1394
Size: 429 2059 3507
Fragments arranged by size:
3507 2059 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2209 4490 4664 65
Size: 1183 961 2281 174 1396
Fragments arranged by size:
2281 1396 1183 961 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4639 4826 1700
Size: 110 2829 187 2869
Fragments arranged by size:
2869 2829 187 110
BsrGI T'GTAC_A
Cuts at: 96 3085 3206 96
Size: 2989 121 2885
Fragments arranged by size:
2989 2885 121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5995
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5995
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5995
BstXI CCAn_nnnn'nTGG
Cuts at: 2674 2674
Size: 5995
BstYI r'GATC_y
Cuts at: 1972 2662 2875 3193 3325 3692 4077 4094 5546
Size: 690 213 318 132 367 385 17 1452
Cuts at: 5546 5632 5644 5990 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 690 385 367 346 318 213
132 86 17 12
BstZ17I GTA'TAC
Cuts at: 2271 2858 2271
Size: 587 5408
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4504
Fragments arranged by size:
4504 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5256
Fragments arranged by size:
5256 683 56
BtsI GCAGTG_nn'
Cuts at: 3511 4305 4325 3511
Size: 794 20 5181
Fragments arranged by size:
5181 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5960
DraI TTT'AAA
Cuts at: 2434 3299 2434
Size: 865 5130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5002 809
Size: 507 3686 1802
Fragments arranged by size:
3686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3442 4331 5960
Size: 54 1425 50 275 18 1612 889 1629
Cuts at: 5960 8
Size: 43
Fragments arranged by size:
1629 1612 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3442 1537
Size: 275 1630 4090
Fragments arranged by size:
4090 1630 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2375 2645 3042 3925 1353
Size: 1022 270 397 883 3423
Fragments arranged by size:
3423 1022 883 397 270
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4582 4956 5102 387
Size: 1064 138 2993 374 146 1280
Fragments arranged by size:
2993 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2626 2997 4058 4655 5442 5710
Size: 393 467 527 371 1061 597 787 268
Cuts at: 5710 1239
Size: 1524
Fragments arranged by size:
1524 1061 787 597 527 467 393 371
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3015 3015
Size: 5995
EcoO109I rG'GnC_Cy
Cuts at: 3020 3020
Size: 5995
EcoRI G'AATT_C
Cuts at: 2481 3159 3304 3682 2481
Size: 678 145 378 4794
Fragments arranged by size:
4794 678 378 145
EcoRV GAT'ATC
Cuts at: 2607 2607
Size: 5995
FspI TGC'GCA
Cuts at: 4501 4501
Size: 5995
HaeII r_GCGC'y
Cuts at: 1678 5142 1678
Size: 3464 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373
Size: 32 135 32 3064 32 42 32 2626
Fragments arranged by size:
3064 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3339 3585 669
Size: 988 281 1401 246 3079
Fragments arranged by size:
3079 1401 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2352 748
Size: 1604 4391
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2894 5109 5293 1305
Size: 1589 2215 184 2007
Fragments arranged by size:
2215 2007 1589 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4173
Fragments arranged by size:
4173 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2757 3953 4312 4471 518
Size: 648 1591 1196 359 159 2042
Fragments arranged by size:
2042 1591 1196 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3411 4088 5236 5481 1606
Size: 1805 677 1148 245 2120
Fragments arranged by size:
2120 1805 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4504
Fragments arranged by size:
4504 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 2895 3084 3353 4898 1132
Size: 97 1666 189 269 1545 2229
Fragments arranged by size:
2229 1666 1545 269 189 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5995
PciI A'CATG_T
Cuts at: 4894 4894
Size: 5995
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5995
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3020 3020
Size: 5995
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5995
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PstI C_TGCA'G
Cuts at: 830 2713 2981 3347 830
Size: 1883 268 366 3478
Fragments arranged by size:
3478 1883 366 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SfcI C'TryA_G
Cuts at: 826 1045 2709 2946 2977 3343 4478 5159 5350
Size: 219 1664 237 31 366 1135 681 191
Cuts at: 5350 826
Size: 1471
Fragments arranged by size:
1664 1471 1135 681 366 237 219 191
31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 3322 1872
Size: 1450 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2466 4099 5000 5262 820
Size: 197 926 523 1633 901 262 1553
Fragments arranged by size:
1633 1553 926 901 523 262 197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 3175 3919 5
Size: 47 3123 744 2081
Fragments arranged by size:
3123 2081 744 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5995
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 2667 3041 3434 513
Size: 552 939 111 552 374 393 3074
Fragments arranged by size:
3074 939 552 552 393 374 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2921 4022 4039 4192 4377 4822 325
Size: 1255 1341 1101 17 153 185 445 1498
Fragments arranged by size:
1498 1341 1255 1101 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3001 3085 3206
Size: 274 80 33 51 1443 1024 84 121
Cuts at: 3206 4241 5953 96
Size: 1035 1712 138
Fragments arranged by size:
1712 1443 1035 1024 274 138 121 84
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2706 2738 5955 5987 2706
Size: 32 3217 32 2714
Fragments arranged by size:
3217 2714 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 3145 3331 3145
Size: 186 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3245 3245
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 3320 1870
Size: 1450 4545
XmnI GAAnn'nnTTC
Cuts at: 4124 4124
Size: 5995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI