Restriction map for
pFN21AE5529
(Circular) (Six-base) MAPSORT of: pFN21AE5529.seq Check: 547 from: 1 to: 5767
pFN21AE5529 5767 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3110 1937
Size: 1173 4594
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 3830 5214 5482 1632
Size: 1049 1149 1384 268 1917
Fragments arranged by size:
1917 1384 1149 1049 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 4666 5643 1203
Size: 3463 977 1327
Fragments arranged by size:
3463 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 2826 1166
Size: 1660 4107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AlwNI CAG_nnn'CTG
Cuts at: 3510 5082 3510
Size: 1572 4195
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2358 3092 1870
Size: 73 415 734 4545
Fragments arranged by size:
4545 734 415 73
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3088 4543 618
Size: 325 217 627 1301 1455 1842
Fragments arranged by size:
1842 1455 1301 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3086 1391
Size: 1695 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2066 2106 2981 928
Size: 971 167 40 875 3714
Fragments arranged by size:
3714 971 875 167 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 2643 2856 1548
Size: 1095 213 4459
Fragments arranged by size:
4459 1095 213
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4378 136
Size: 107 122 71 631 605 2706 1525
Fragments arranged by size:
2706 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5762 5762
Size: 5767
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 3828 4984 1790
Size: 2038 1156 2573
Fragments arranged by size:
2573 2038 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 4427 1239
Size: 1202 1986 2579
Fragments arranged by size:
2579 1986 1202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4430 882
Size: 617 2931 2219
Fragments arranged by size:
2931 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3574 3956 4591 275
Size: 53 83 186 936 2041 382 635 1451
Fragments arranged by size:
2041 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 1186
Size: 934 4833
BseYI C'CCAG_C
Cuts at: 4970 5466 4970
Size: 496 5271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2850 1163
Size: 573 1114 4080
Fragments arranged by size:
4080 1114 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3217 3978 5006 1540
Size: 275 186 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2161 2907 3828 3913 4984 2161
Size: 746 921 85 1071 2944
Fragments arranged by size:
2944 1071 921 746 85
BsmI GAATG_Cn'
Cuts at: 2138 3278 3371 2138
Size: 1140 93 4534
Fragments arranged by size:
4534 1140 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2161 2907 3086 3828 3913 4984 1391
Size: 399 371 746 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 746 742 399 371 179 85
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3122 844
Size: 2278 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 1394
Size: 429 1831 3507
Fragments arranged by size:
3507 1831 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2199 4262 4436 65
Size: 1183 951 2063 174 1396
Fragments arranged by size:
2063 1396 1183 951 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4411 4598 1700
Size: 110 2601 187 2869
Fragments arranged by size:
2869 2601 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 2606 3827 4839 2606
Size: 1221 1012 3534
Fragments arranged by size:
3534 1221 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5767
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2169 3097 3464 3849 3866 5318 5404 5416
Size: 197 928 367 385 17 1452 86 12
Cuts at: 5416 5762 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 928 385 367 346 197 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2085 2442 513
Size: 552 1020 357 3838
Fragments arranged by size:
3838 1020 552 357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 2133 3283 4077 4097 2133
Size: 1150 794 20 3803
Fragments arranged by size:
3803 1150 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2317 2352 529
Size: 35 1753 35 3944
Fragments arranged by size:
3944 1753 35 35
DraI TTT'AAA
Cuts at: 2374 3071 2374
Size: 697 5070
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2510 4774 809
Size: 507 1194 2264 1802
Fragments arranged by size:
2264 1802 1194 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2919 3214 4103
Size: 54 1425 50 275 18 1089 295 889
Cuts at: 4103 5732 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1089 889 295 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2888 3697 1353
Size: 1535 809 3423
Fragments arranged by size:
3423 1535 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2978 4354 4728 4874 387
Size: 1064 138 1389 1376 374 146 1280
Fragments arranged by size:
1389 1376 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 2681 3830 4427 5214 5482 1239
Size: 393 809 240 1149 597 787 268 1524
Fragments arranged by size:
1524 1149 809 787 597 393 268 240
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 4914 1678
Size: 3236 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4408 4440 4482 4514 1373
Size: 32 135 32 2836 32 42 32 2626
Fragments arranged by size:
2836 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2376 748
Size: 1628 4139
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4881 5065 1305
Size: 3576 184 2007
Fragments arranged by size:
3576 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2921 10
Size: 54 1425 343 1089 2856
Fragments arranged by size:
2856 1425 1089 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 2826 3725 4084 4243 518
Size: 648 1224 436 899 359 159 2042
Fragments arranged by size:
2042 1224 899 648 436 359 159
MspA1I CmG'CkG
Cuts at: 1606 2294 3007 3183 3860 5008 5253 1606
Size: 688 713 176 677 1148 245 2120
Fragments arranged by size:
2120 1148 713 688 677 245 176
NcoI C'CATG_G
Cuts at: 513 1065 2085 2442 513
Size: 552 1020 357 3838
Fragments arranged by size:
3838 1020 552 357
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 2408 3125 4670 1132
Size: 97 1179 717 1545 2229
Fragments arranged by size:
2229 1545 1179 717 97
PasI CC'CwG_GG
Cuts at: 1348 2144 1348
Size: 796 4971
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2667 1108
Size: 1559 4208
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PstI C_TGCA'G
Cuts at: 830 3119 830
Size: 2289 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2294 3007 2294
Size: 713 5054
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2908 3115 4250 4931 5122 826
Size: 219 1863 207 1135 681 191 1471
Fragments arranged by size:
1863 1471 1135 681 219 207 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 2360 3094 1872
Size: 488 734 4545
Fragments arranged by size:
4545 734 488
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3871 4772 5034 820
Size: 197 926 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 2408 3125 1132
Size: 1276 717 3774
Fragments arranged by size:
3774 1276 717
SspI AAT'ATT
Cuts at: 5 52 2094 2926 3691 5
Size: 47 2042 832 765 2081
Fragments arranged by size:
2081 2042 832 765 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 2085 2442 2666 2897 3018 3206 513
Size: 552 1020 357 224 231 121 188 3074
Fragments arranged by size:
3074 1020 552 357 231 224 188 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2649 3794 3811 3964 4149 4594 325
Size: 1255 1069 1145 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1145 1069 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4013 5725 96
Size: 274 80 33 51 1443 2036 1712 138
Fragments arranged by size:
2036 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2597 2629 5727 5759 2597
Size: 32 3098 32 2605
Fragments arranged by size:
3098 2605 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2206 2206
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 2358 3092 1870
Size: 488 734 4545
Fragments arranged by size:
4545 734 488
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI