Restriction map for
pFN21AE5513
(Circular) (Six-base) MAPSORT of: pFN21AE5513.seq Check: 9586 from: 1 to: 5956
pFN21AE5513 5956 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 2097 4083 4456 1563
Size: 534 1986 373 3063
Fragments arranged by size:
3063 1986 534 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2495 4019 5403 5671 1632
Size: 863 1524 1384 268 1917
Fragments arranged by size:
1917 1524 1384 863 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AlwNI CAG_nnn'CTG
Cuts at: 2174 2840 3699 5271 2174
Size: 666 859 1572 2859
Fragments arranged by size:
2859 1572 859 666
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 2761 3265 3491 3643 5752 1493
Size: 1268 504 226 152 2109 1697
Fragments arranged by size:
2109 1697 1268 504 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3052 3281 1870
Size: 73 1109 229 4545
Fragments arranged by size:
4545 1109 229 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3032 3277 4732 618
Size: 325 217 627 1245 245 1455 1842
Fragments arranged by size:
1842 1455 1245 627 325 245 217
BanII G_rGCy'C
Cuts at: 1391 3089 3275 1391
Size: 1698 186 4072
Fragments arranged by size:
4072 1698 186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2419 2453 4147 4181 1903
Size: 34 482 34 1694 34 3678
Fragments arranged by size:
3678 1694 482 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4567 136
Size: 107 122 71 631 605 337 2558 1525
Fragments arranged by size:
2558 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 4017 5173 1790
Size: 2227 1156 2573
Fragments arranged by size:
2573 2227 1156
BmgBI CAC'GTC
Cuts at: 1318 2600 1318
Size: 1282 4674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3106 3687 4645
Size: 416 53 83 399 24 1689 581 958
Cuts at: 4645 442
Size: 1753
Fragments arranged by size:
1753 1689 958 581 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2727 2727
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4616 1239
Size: 3377 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2843 3197 4619 882
Size: 617 1344 354 1422 2219
Fragments arranged by size:
2219 1422 1344 617 354
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 2109 5950 2109
Size: 3841 2115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3763 4145 4780 275
Size: 53 83 186 936 2230 382 635 1451
Fragments arranged by size:
2230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 2906 2936 1178
Size: 30 165 30 897 30 576 30 4198
Fragments arranged by size:
4198 897 576 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3215 1186
Size: 2029 3927
BseYI C'CCAG_C
Cuts at: 3080 5159 5655 3080
Size: 2079 496 3381
Fragments arranged by size:
3381 2079 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3011 1163
Size: 573 1275 4108
Fragments arranged by size:
4108 1275 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 2658 4017 4102 5173 2067
Size: 591 1359 85 1071 2850
Fragments arranged by size:
2850 1359 1071 591 85
BsmI GAATG_Cn'
Cuts at: 2555 2992 3467 3560 2555
Size: 437 475 93 4951
Fragments arranged by size:
4951 475 437 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2658 3089 3275 4017 4102 5173
Size: 399 277 591 431 186 742 85 1071
Cuts at: 5173 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 591 431 399 277 186
85
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2836 3203 3311 844
Size: 1992 367 108 3489
Fragments arranged by size:
3489 1992 367 108
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2860 2515
Size: 345 5611
BstYI r'GATC_y
Cuts at: 1972 2604 2729 3183 3286 3653 4038 4055 5507
Size: 632 125 454 103 367 385 17 1452
Cuts at: 5507 5593 5605 5951 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 632 454 385 367 346 125
103 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5404
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 2437 3023 3472 4266 4286 2437
Size: 586 449 794 20 4107
Fragments arranged by size:
4107 794 586 449 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2642 4963 809
Size: 507 1326 2321 1802
Fragments arranged by size:
2321 1802 1326 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 3403 4292
Size: 54 1425 50 275 18 306 1267 889
Cuts at: 4292 5921 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1267 889 306 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2893 3886 1353
Size: 1540 993 3423
Fragments arranged by size:
3423 1540 993
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2495 4019 4616 5403 5671 1239
Size: 393 863 1524 597 787 268 1524
Fragments arranged by size:
1524 1524 863 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2474 2474
Size: 5956
EcoO109I rG'GnC_Cy
Cuts at: 2857 2857
Size: 5956
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 5956
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 5103 1678
Size: 3425 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2589 2621 4597 4629 4671
Size: 32 135 32 1017 32 1976 32 42
Cuts at: 4671 4703 1373
Size: 32 2626
Fragments arranged by size:
2626 1976 1017 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2095 2206 3300 3546 669
Size: 988 281 157 111 1094 246 3079
Fragments arranged by size:
3079 1094 988 281 246 157 111
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 2095 3546 2095
Size: 1451 4505
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2138 10
Size: 54 1425 343 306 3828
Fragments arranged by size:
3828 1425 343 306 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2055 2058 2349 2513 3914 4273 4432
Size: 648 889 3 291 164 1401 359 159
Cuts at: 4432 518
Size: 2042
Fragments arranged by size:
2042 1401 889 648 359 291 164 159
3
MspA1I CmG'CkG
Cuts at: 1606 3372 4049 5197 5442 1606
Size: 1766 677 1148 245 2120
Fragments arranged by size:
2120 1766 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5404
NdeI CA'TA_TG
Cuts at: 387 2950 3046 387
Size: 2563 96 3297
Fragments arranged by size:
3297 2563 96
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3314 4859 1132
Size: 97 2085 1545 2229
Fragments arranged by size:
2229 2085 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2541 1108
Size: 1433 4523
PfoI T'CCnGG_A
Cuts at: 3240 3240
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2069 2101 2568 2600 4025 4057 5538 5570 2069
Size: 32 467 32 1425 32 1481 32 2455
Fragments arranged by size:
2455 1481 1425 467 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PstI C_TGCA'G
Cuts at: 830 3308 830
Size: 2478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 3304 4439 5120 5311 826
Size: 219 2259 1135 681 191 1471
Fragments arranged by size:
2259 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3054 3283 1872
Size: 1182 229 4545
Fragments arranged by size:
4545 1182 229
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4060 4961 5223 820
Size: 197 926 2117 901 262 1553
Fragments arranged by size:
2117 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 2662 3880 5
Size: 47 2610 1218 2081
Fragments arranged by size:
2610 2081 1218 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2853 3109 3395 513
Size: 552 1788 256 286 3074
Fragments arranged by size:
3074 1788 552 286 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2735 3983 4000 4153 4338 4783 325
Size: 1255 1155 1248 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1248 1155 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2198 2358 3039
Size: 274 80 33 51 1443 221 160 681
Cuts at: 3039 4202 5914 96
Size: 1163 1712 138
Fragments arranged by size:
1712 1443 1163 681 274 221 160 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2395 2427 2906 2938 5916 5948 2395
Size: 32 479 32 2978 32 2403
Fragments arranged by size:
2978 2403 479 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3238 3238
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3052 3281 1870
Size: 1182 229 4545
Fragments arranged by size:
4545 1182 229
XmnI GAAnn'nnTTC
Cuts at: 2084 4085 2084
Size: 2001 3955
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI