Restriction map for
pFN21AE5502
(Circular) (Six-base) MAPSORT of: pFN21AE5502.seq Check: 3902 from: 1 to: 5887
pFN21AE5502 5887 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3230 1937
Size: 1293 4594
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2823 3950 5334 5602 1632
Size: 1191 1127 1384 268 1917
Fragments arranged by size:
1917 1384 1191 1127 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 4786 5763 1203
Size: 3583 977 1327
Fragments arranged by size:
3583 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AlwNI CAG_nnn'CTG
Cuts at: 3630 5202 3630
Size: 1572 4315
ApaLI G'TGCA_C
Cuts at: 2157 3944 5100 2157
Size: 1787 1156 2944
Fragments arranged by size:
2944 1787 1156
ApoI r'AATT_y
Cuts at: 1493 2703 3028 3196 3422 3574 5683 1493
Size: 1210 325 168 226 152 2109 1697
Fragments arranged by size:
2109 1697 1210 325 226 168 152
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 2745 3212 1870
Size: 73 802 467 4545
Fragments arranged by size:
4545 802 467 73
BamHI G'GATC_C
Cuts at: 3217 3584 3217
Size: 367 5520
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3208 4663 618
Size: 325 217 627 1421 1455 1842
Fragments arranged by size:
1842 1455 1421 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3206 1391
Size: 1815 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4916
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4078 4112 1903
Size: 34 2141 34 3678
Fragments arranged by size:
3678 2141 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 3160 1548
Size: 1612 4275
BfrBI ATG'CAT
Cuts at: 2542 2542
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2512 5882 2512
Size: 3370 2517
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 2161 2429 3948 5104 1790
Size: 371 268 1519 1156 2573
Fragments arranged by size:
2573 1519 1156 371 268
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5887
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3618 4576 442
Size: 416 53 83 399 24 2201 958 1753
Fragments arranged by size:
2201 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2352 4547 1239
Size: 1113 2195 2579
Fragments arranged by size:
2579 2195 1113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2481 2667 4012 4877 5175 2481
Size: 186 1345 865 298 3193
Fragments arranged by size:
3193 1345 865 298 186
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2447 2828 4550 882
Size: 617 948 381 1722 2219
Fragments arranged by size:
2219 1722 948 617 381
BsaAI yAC'GTr
Cuts at: 493 1204 3678 493
Size: 711 2474 2702
Fragments arranged by size:
2702 2474 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3694 4076 4711 275
Size: 53 83 186 936 2161 382 635 1451
Fragments arranged by size:
2161 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4320 4718 4992 5139 5550 4320
Size: 398 274 147 411 4657
Fragments arranged by size:
4657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5662
Fragments arranged by size:
5662 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2265 2992 1186
Size: 1079 727 4081
Fragments arranged by size:
4081 1079 727
BseYI C'CCAG_C
Cuts at: 2075 5090 5586 2075
Size: 3015 496 2376
Fragments arranged by size:
3015 2376 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5314
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2070 3337 4098 5126 1540
Size: 275 186 69 1267 761 1028 2301
Fragments arranged by size:
2301 1267 1028 761 275 186 69
BsiHKAI G_wGCw'C
Cuts at: 2161 3948 4033 5104 2161
Size: 1787 85 1071 2944
Fragments arranged by size:
2944 1787 1071 85
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2161 2429 3206 3948 4033 5104 1391
Size: 399 371 268 777 742 85 1071 2174
Fragments arranged by size:
2174 1071 777 742 399 371 268 85
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 3169 3776 5506 3169
Size: 607 1730 3550
Fragments arranged by size:
3550 1730 607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3242 844
Size: 2398 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4382 4556 65
Size: 1183 3134 174 1396
Fragments arranged by size:
3134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 3054 3947 4959 3054
Size: 893 1012 3982
Fragments arranged by size:
3982 1012 893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5887
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5887
BstYI r'GATC_y
Cuts at: 1972 2512 3217 3584 3969 3986 5438 5524 5536
Size: 540 705 367 385 17 1452 86 12
Cuts at: 5536 5882 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 705 540 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 2397 3403 4197 4217 2397
Size: 1006 794 20 4067
Fragments arranged by size:
4067 1006 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2386 2421 529
Size: 35 1822 35 3995
Fragments arranged by size:
3995 1822 35 35
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DraIII CAC_nnn'GTG
Cuts at: 2861 2861
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2066 4894 809
Size: 507 750 2828 1802
Fragments arranged by size:
2828 1802 750 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2302 2394 3334
Size: 54 1425 50 275 18 472 92 940
Cuts at: 3334 4223 5852 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 940 889 472 275 92 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2565 2969 3817 1353
Size: 1212 404 848 3423
Fragments arranged by size:
3423 1212 848 404
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4474 4848 4994 387
Size: 1064 138 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 2823 3950 4547 5334 5602 1239
Size: 393 720 471 1127 597 787 268 1524
Fragments arranged by size:
1524 1127 787 720 597 471 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2130 2130
Size: 5887
EcoRI G'AATT_C
Cuts at: 3196 3574 3196
Size: 378 5509
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 5034 1678
Size: 3356 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2649 2681 3035 3067 4528
Size: 32 135 32 1077 32 354 32 1461
Cuts at: 4528 4560 4602 4634 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1461 1077 354 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 3477 669
Size: 988 281 1293 246 3079
Fragments arranged by size:
3079 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5887
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 2093 2778 3486 2093
Size: 685 708 4494
Fragments arranged by size:
4494 708 685
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1992 2070 2082 3114 5001 5185 1305
Size: 687 78 12 1032 1887 184 2007
Fragments arranged by size:
2007 1887 1032 687 184 78 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2304 2396 10
Size: 54 1425 343 472 92 3501
Fragments arranged by size:
3501 1425 472 343 92 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3168 3845 4204 4363 518
Size: 648 2002 677 359 159 2042
Fragments arranged by size:
2042 2002 677 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2672 2801 3303 3980 5128 5373 1606
Size: 483 583 129 502 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 583 502 483 245 129
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
NdeI CA'TA_TG
Cuts at: 387 2354 387
Size: 1967 3920
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NsiI A_TGCA'T
Cuts at: 2544 2544
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 2157 3122 3245 4790 1132
Size: 97 928 965 123 1545 2229
Fragments arranged by size:
2229 1545 965 928 123 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5887
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 2831 3457 2831
Size: 626 5261
PstI C_TGCA'G
Cuts at: 830 2373 3239 830
Size: 1543 866 3478
Fragments arranged by size:
3478 1543 866
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SexAI A'CCwGG_T
Cuts at: 2857 2857
Size: 5887
SfcI C'TryA_G
Cuts at: 826 1045 2369 3235 4370 5051 5242 826
Size: 219 1324 866 1135 681 191 1471
Fragments arranged by size:
1471 1324 1135 866 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2496 2682 3991 4892 5154 820
Size: 197 926 553 186 1309 901 262 1553
Fragments arranged by size:
1553 1309 926 901 553 262 197 186
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 3086 1517
Size: 1569 4318
StyI C'CwwG_G
Cuts at: 513 1065 2004 3087 3326 513
Size: 552 939 1083 239 3074
Fragments arranged by size:
3074 1083 939 552 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2089 3914 3931 4084 4269 4714 325
Size: 1255 509 1825 17 153 185 445 1498
Fragments arranged by size:
1825 1498 1255 509 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4133 5845 96
Size: 274 80 33 51 1443 2156 1712 138
Fragments arranged by size:
2156 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5887
XbaI T'CTAG_A
Cuts at: 2515 3223 2515
Size: 708 5179
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 4016 4016
Size: 5887
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI